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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 高燕玉 | |
| dc.contributor.author | Jung Liu | en |
| dc.contributor.author | 劉蓉 | zh_TW |
| dc.date.accessioned | 2021-05-20T20:04:58Z | - |
| dc.date.available | 2009-08-18 | |
| dc.date.available | 2021-05-20T20:04:58Z | - |
| dc.date.copyright | 2009-08-18 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-08-14 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/8954 | - |
| dc.description.abstract | 微衛星體序列是以 1-6 bp 為組成單位的簡單重複性序列，普遍存在於真核生物的基因組中。本實驗利用 (GA)11為引子進行單引子聚合酶連鎖反應，從蝴蝶蘭 Phalaenopsis stuartiana 基因組分離出 15 個富含 (GA)n 微衛星體序列的選殖體，其中 14 個為複合型，序列長度最長為 726 bp，最短為 92 bp;而 GA 重複次數最高達 28 次，TC重複次數最高達 32 次，序列間的相似度介於 35.98-78.57%，由 TIGR 資料庫比對結果，與許多植物之重複性序列相類似。挑選其中 8 個選殖體，以及朵麗蘭的 DpGA2 選殖體進行螢光原位雜交，結果訊號皆集中於 P. stuartiana 所有染色體的中節，且強度大致相同，與選殖體 DpGA2 只位於朵麗蘭一對染色體的中節不相吻合。選殖體 PstGA3 在 P. violacea 及朵麗蘭染色體上皆為散佈型，與前人研究 (GA)11 位於朵麗蘭中節的結果不符，而 PstGA16 在 P. amboinensis 為散佈型，而在 P. mannii 則聚集於中節，則與過去研究結果相符。(GA)n 微衛星體序列是否為 P. stuartiana 具功能的中節序列，將來可以免疫染色法或免疫沉澱法加以證實。 | zh_TW |
| dc.description.abstract | Microsatellites are tandemly repeated tracts of DNA composed of 1–6 base pair long units and ubiquitous in eukaryotic genomes. In this study, fifteen clones riched in (GA)n microsatellites were isolated from Phalaenopsis stuartiana by single-primer PCR using (GA)11 as a primer. All clones were compound except one imperfect repeat sequences. The insert size varied from 28 bp to 726 bp, and the maximum repeat numbers of GA and TC were 28 and 32 in these clones. The sequence identity ranged from 35.98 to 78.57% among them. According to the TIGR database, these sequences showed similarities with repetitive sequences in many plants. Nine clones, eight clones from P. stuartiana and DpGA2 from P. pulcherrima, were selected for physical mapping by fluorescence in situ hybridization (FISH). The signals were clustered at all centromeres of P. stuartiana chromosomes. It was found a quite exceptional distribution of DpGA2 which was clustered at the centromeric regions of only one pair of P. pulcherrima chromosomes. In situ hybridization to P. violacea and P. pulcherrima chromosomes with clone PstGA3 revealed dispersed signals. The result of the former but the latter was consistent with the previous study using synthetic oligonucleotides (GA)11 as a probe. The signals of the clone PstGA16 were scattered along the chromosomes of P. amboienesis, but clustered at the centromeres of P. mannii. It was well correlated with the previous study. Whether (GA)n microsatellite is the functional centromeric element of P. stuartiana remained to resolve by immunostaining or chromatin immunoprecipitation in the future. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-20T20:04:58Z (GMT). No. of bitstreams: 1 ntu-98-R95b43019-1.pdf: 1928605 bytes, checksum: b403f967f9670e11dd886893554f6740 (MD5) Previous issue date: 2009 | en |
| dc.description.tableofcontents | 中文摘要---------------------------------------------i
英文摘要---------------------------------------------ii 壹、 前言--------------------------------------------1 貳、 材料方法 一、 植物材料-------------------------------------8 二、 DNA 的抽取----------------------------------8 三、 含 (GA)n 微衛星體序列選殖體的選殖------------10 四、 南方氏雜交----------------------------------13 五、 (GA)n 微衛星體序列的定序與分析---------------16 六、 螢光原位雜交--------------------------------16 參、 結果 一、 含 (GA)n 微衛星體序列的選殖------------------21 二、 含 (GA)n 微衛星體序列選殖體之定序分析--------24 三、 螢光原位雜交---------------------------------29 肆、 討論---------------------------------------------37 伍、 參考文獻-----------------------------------------42 陸、 附錄-----------------------------------------------48 | |
| dc.language.iso | zh-TW | |
| dc.subject | 蝴蝶蘭 | zh_TW |
| dc.subject | Phalaenopsis stuartiana | zh_TW |
| dc.subject | (GA)n 微衛星體序列 | zh_TW |
| dc.title | 蝴蝶蘭 Phalaenopsis stuartiana 之 (GA)n 微衛星體序列的選殖與定位 | zh_TW |
| dc.title | Cloning and physical mapping of (GA)n microsatellites in Phalaenopsis stuartiana | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 鍾美珠,吳文鑾 | |
| dc.subject.keyword | 蝴蝶蘭,Phalaenopsis stuartiana,(GA)n 微衛星體序列, | zh_TW |
| dc.subject.keyword | Phalaenopsis stuartiana,(GA)n microsatellites, | en |
| dc.relation.page | 51 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2009-08-14 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 分子與細胞生物學研究所 | zh_TW |
| 顯示於系所單位： | 分子與細胞生物學研究所 | |
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