請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/89416完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 萬灼華 | zh_TW |
| dc.contributor.advisor | Cho-Hua Wan | en |
| dc.contributor.author | 鄭穎謙 | zh_TW |
| dc.contributor.author | Ying-Chien Cheng | en |
| dc.date.accessioned | 2023-09-07T16:55:09Z | - |
| dc.date.available | 2025-07-17 | - |
| dc.date.copyright | 2023-09-11 | - |
| dc.date.issued | 2023 | - |
| dc.date.submitted | 2023-07-17 | - |
| dc.identifier.citation | 1. Council of Agriculture. 2021. Animal Protection Act. ROC. https://law.moj.gov.tw/ENG/LawClass/LawAll.aspx?pcode=M0060027.
2. Baker DG. 1998. Natural pathogens of laboratory mice, rats, and rabbits and their effects on research. Clin Microbiol Rev. 11:231-66. doi: 10.1128/CMR.11.2.231. PMID: 9564563. PMCID: PMC106832. 3. Baker DG. 2007. Parasites of rats and mice, p.359–368. In: Baker, DG, editor. Flynn's Parasites of Laboratory Animals, 2nd ed. Ames (IA): Blackwell Publishing. 4. Ball-Goodrich LJ, Johnson E. 1994. Molecular characterization of a newly recognized mouse parvovirus. J Virol. 68:6476-6486. doi: 10.1128/JVI.68.10.6476-6486.1994. PMID: 8083985. PMCID: PMC237068. 5. Bernier CR, Petrov AS, Waterbury CC, Jett J, Li F, Freil LE, Xiong X, Wang L, Migliozzi BLR, Hershkovits E, Xue Y, Hsiao C, Bowman JC, Harvey SC, Grover MA, Wartelle ZJ, Williams LD. 2014. RiboVision suite for visualization and analysis of ribosomes. Faraday Discuss. 169:195-207. DOI: 10.1039/c3fd00126a. 6. Bochkov AV. 2009. Mites of the family Myobiidae (Acari: Prostigmata) parasitizing rodents of the former USSR. Acarina. 17:109-169. 7. Bochkov AV. 2010. A review of mammal-associated Psoroptidia (Acariformes: Astigmata). Acarina. 18:99-260. 8. Chen CH, Wang MH, Wan CH. 2020. Multiplex polymerase chain reaction assay for the detection of five common endoparasite infestations in laboratory rodents. Taiwan Vet J. 46:123-132. doi: 10.1142/S1682648520500110. 9. Chou CL, Cheng YC, Wang MH, Wan CH. 2020. Novel PCR assays for three fur mites in naturally infected mice without animal sacrifice. Taiwan Vet J. 46:133-142. https://doi.org/10.1142/S1682648520500122. 10. Council of Agriculture. 2018. Guideline for the care and use of laboratory animals, Chinese-Taipei society of laboratory animal sciences, Taipei, ROC. 11. Docampo R. 2017. ZooBank registration requirement. J Eukaryot Microbiol. 64:728. doi: 10.1111/jeu.12477. PMID: 29139237. 12. Feldman SH, Ntenda AM. 2011. Phylogenetic analysis of Myobia musculi (Schranck, 1781) by using the 18S small ribosomal subunit sequence. Comp Med. 61:484-491. PMID: 22330574. PMCID: PMC3236689. 13. Gerwin PM, Arbona RJR, Riedel ER, Henderson KS, Lipman NS. 2017. PCR testing of IVC filter tops as a method for detecting murine pinworms and fur mites. J Am Assoc Lab Anim Sci. 56:752-761. PMID: 29256370. PMCID: PMC5710154. 14. Grove KA, Smith PC, Booth CJ, Compton SR. 2012. Age-associated variability in susceptibility of Swiss Webster mice to MPV and other excluded murine pathogens. J Am Assoc Lab Anim Sci. 51:789-796. PMID: 23294885; PMCID: PMC3508183. 15. Hanson WH, Taylor K, Taylor DK. 2021. PCR testing of media placed in soiled bedding as a method for mouse colony health surveillance. J Am Assoc Lab Anim Sci. 60:306-310. doi: 10.30802/AALAS-JAALAS-20-000096. PMID: 33952384. PMCID: PMC8145126. 16. Johnston NA, Trammell RA, Ball-Kell S, Verhulst S, Toth LA. 2009. Assessment of immune activation in mice before and after eradication of mite infestation. J Am Assoc Lab Anim Sci. 48:371-377. PMID: 19653944. PMCID: PMC2715926. 17. Karlsson EM, Pearson LM, Kuzma KM, Burkholder TH. 2014. Combined evaluation of commonly used techniques, including PCR, for diagnosis of mouse fur mites. J Am Assoc Lab Anim. Sci. 53:69-73. PMID: 24411782. PMCID: PMC3894650. 18. Klimov PB, OConnor BM, Chetverikov PE, Bolton SJ, Pepato AR, Mortazavi AL, Tolstikov AV, Bauchan GR, Ochoa R. 2018. Comprehensive phylogeny of acariform mites (Acariformes) provides insights on the origin of the four-legged mites (Eriophyoidea), a long branch. Mol Phylogenet Evol. 119:105-117. doi: 10.1016/j.ympev.2017.10.017. PMID: 29074461. 19. Körner C, Miller M, Brielmeier M. 2019. Detection of murine astrovirus and Myocoptes musculinus in individually ventilated caging systems: Investigations to expose suitable detection methods for routine hygienic monitoring. PLoS One. 14:e0221118. doi: 10.1371/journal.pone.0221118. PMID: 31408494. PMCID: PMC6692027. 20. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol. 35:1547–1549. doi:10.1093/molbev/msy096. 21. Lee MA, Shen Z, Holcombe HR, Ge Z, Franklin EG, Arbona RJR, Lipman NS, Fox JG, Sheh A. 2019. Detection of Myocoptes musculinus in fur swab and fecal samples by using PCR analysis. J Am Assoc Lab Anim Sci. 58:796-801. doi: 10.30802/AALAS-JAALAS-19-000046. PMID: 31662161; PMCID: PMC6926404. 22. Liao JR, Ho CC, Ko CC. 2021. Predatory mites (Acari: Mesostigmata: Phytoseiidae) intercepted from samples imported to Taiwan, with description of a new species. Zootaxa. 4927:301-330. doi: 10.11646/zootaxa.4927.3.1. PMID: 33756698. 23. Lindstrom KE, Carbone LG, Kellar DE, Mayorga MS, Wilkerson JD. 2011. Soiled bedding sentinels for the detection of fur mites in mice. J Am Assoc Lab Anim Sci. 50:54-60. PMID: 21333164. PMCID: PMC3035404. 24. Matsuda T, Fukumoto C, Hinomoto N, Gotoh T. 2013. DNA-based identification of spider mites: Molecular evidence for cryptic species of the genus Tetranychus (Acari: Tetranychidae). J Econ Entomol. 106:463–472. doi: 10.1603/EC12328. 25. Morita E, Kaneko S, Hiragun T, Shindo H, Tanaka T, Furukawa T, Nobukiyo A, Yamamoto S. 1999. Fur mites induce dermatitis associated with IgE hyperproduction in an inbred strain of mice, NC/Kuj. J Dermatol Sci. 19:37-43. doi:10.1016/s0923-1811(98)00047-4. 26. Morita T, Ohmi A, Kiwaki A, Ike K, Nagata K. 2018. A new stubby species of demodectic mite (Acari: Demodicidae) from the domestic dog (Canidae). J Med Entomol. 55:323-328. doi: 10.1093/jme/tjx226. PMID: 29309708. 27. Mullen GR, OConnor BM. 2019. Mites (Acari), p.533-602. In: Mullen GR, Durden LA, editors. Medical and Veterinary Entomology, 3rd ed. Academic Press. ISBN 9780128140437. doi: 10.1016/B978-0-12-814043-7.00026-1. 28. National Research Council (US) Committee on Infectious Diseases of Mice and Rats. 1991. Infectious diseases of mice and rats. National Academies Press (US). doi:10.17226/1429. 29. Petrov AS, Bernier CR, Gulen B, Waterbury CC, Hershkovits E, Hsiao C, Harvey SC, Hud NV, Fox GE, Wartell RM, Williams LD. 2014a. Secondary structures of rRNAs from all three domains of life. PLoS One. 9:e88222. doi: 10.1371/journal.pone.0088222. PMID: 24505437. PMCID: PMC3914948. 30. Petrov AS, Bernier CR, Hsiao C, Norris AM, Kovacs NA, Waterbury CC, Stepanov, VG, Harvey SC, Fox GE, Wartell RM, Hud NV, Williams LD. 2014b. Evolution of the ribosome at atomic resolution. Proc Natl Acad Sci USA. 111:10251-10256. doi: 10.1073/pnas.1407205111. PMID: 24982194. PMCID: PMC4104869. 31. Petrov AS, Gulen B, Norris AM, Kovacs NA, Bernier CR, Lanier KA, Fox GE, Harvey SC, Wartell RM, Hud NV, Williams LD. 2015. History of the ribosome and the origin of translation. Proc Natl Acad Sci USA. 112:15396-15401. doi:10.1073/pnas.1509761112. 32. Pochanke V, Hatak S, Hengartner H, Zinkernagel RM, McCoy KD. 2006. Induction of IgE and allergic-type responses in fur mite-infested mice. Eur J Immunol. 36:2434-2445. doi: 10.1002/eji.200635949. PMID: 16909433. 33. Poppe SA. 1896. Beitrag zur Kenntnis der Gattung Myobia v. Heyden. Zool Anz. 19:327–333. 34. Ramesh M, Woolford Jr JL. 2016. Eukaryote-specific rRNA expansion segments function in ribosome biogenesis. RNA. 22:1153-1162. doi: 10.1261/rna.056705.116. PMID: 27317789. PMCID: PMC4931108. 35. Roediger B, Lee Q, Tikoo S, Cobbin JCA, Henderson JM, Jormakka M, O’Rourke MB, Padula MP, Pinello N, Henry M, Wynne M, Santagostino SF, Brayton CF, Rasmussen L, Lisowski L, Tay SS, Harris DC, Bertram JF, Dowling JP, Bertolino P, Lai JH, Wu W, Bachovchin WW, Wong, JJL., Gorrell MD, Shaban B, Holmes EC, Jolly CJ, Monette S, Weninger W. 2018. An atypical parvovirus drives chronic tubulointerstitial nephropathy and kidney fibrosis. Cell. 175:530-543.e24. doi: 10.1016/j.cell.2018.08.013. PMID: 30220458. PMCID: PMC6800251. 36. Sastre N, Calvete O, Martínez-Vargas J, Medarde N, Casellas J, Altet L, Sánchez A, Francino O, Ventura J. 2018. Skin mites in mice (Mus musculus): high prevalence of Myobia sp. (Acari, Arachnida) in Robertsonian mice. Parasitol Res. 117:2139-2148. PUBMED:29728826. doi: 10.1007/s00436-018-5901-z. 37. Schneider CA, Rasband WS, Eliceiri KW. 2012. NIH Image to ImageJ: 25 years of image analysis. Nat Methods. 9:671-675. doi: 10.1038/nmeth.2089. PMID: 22930834. PMCID: PMC5554542. 38. Schrank PP. 1781. Pediculus muris musculi. Enumeratio Insectorum Austriae Indigent. 1058:513. 39. Thigpen JE, Lebetkin EH, Dawes ML, Amyx HL, Caviness GF, Sawyer BA, Blackmore DE. 1989. The use of dirty bedding for detection of murine pathogens in sentinel mice. Lab Anim Sci. 39:324-327. PMID: 2548034. 40. Trifinopoulos J, Nguyen LT, Haeseler A, Minh BQ. 2016. W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Res. 44:W232-W235. doi: 10.1093/nar/gkw256. PMID: 27084950. PMCID: PMC4987875. 41. Wan CH, Söderlund-Venermo M, Pintel DJ, Riley LK. 2002. Molecular characterization of three newly recognized rat parvoviruses. J Gen Virol. 83:2075-2083. doi: 10.1099/0022-1317-83-8-2075. PMID: 12124471. 42. Weiss EE, Evans KD, Griffey SM. 2012. Comparison of a fur mite PCR assay and the tape test for initial and posttreatment diagnosis during a natural infection. J Am Assoc Lab Anim Sci. 51:574-578. PMID: 23312085. PMCID: PMC3447445. 43. Wobus CE, Karst SM, Thackray LB, Chang KO, Sosnovtsev SV, Belliot G, Krug A, Mackenzie JM, Green KY, Virgin IV HW. 2004. Replication of Norovirus in cell culture reveals a tropism for dendritic cells and macrophages. PLoS Biol. 2:e432. doi: 10.1371/journal.pbio.0020432. PMID: 15562321; PMCID: PMC532393. 44. Zhao YE, Xu JR, Hu L, Wu LP, Wang ZH. 2012. Complete sequence analysis of 18S rDNA based on genomic DNA extraction from individual Demodex mites (Acari: Demodicidae). Exp Parasitol. 131:45-51. doi: 10.1016/j.exppara.2012.02.025. PMID: 22414329. 45. Zhao YE, Wang ZH, Xu Y, Wu LP, Hu L. 2013. Secondary structure prediction for complete rDNA sequences (18S, 5.8S, and 28S rDNA) of Demodex folliculorum, and comparison of divergent domains structures across Acari. Exp Parasitol. 135:370-381. doi: 10.1016/j.exppara.2013.07.025. PMID: 23954189. 46. Zhao Y, Zhang WY, Wang RL, Niu DL. 2020. Divergent domains of 28S ribosomal RNA gene: DNA barcodes for molecular classification and identification of mites. Parasit Vectors. 13:251. doi: 10.1186/s13071-020-04124-z. PMID: 32404192. PMCID: PMC7222323. | - |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/89416 | - |
| dc.description.abstract | Myobia murismusculi (又名Myobia musculi, MOB)、 Radfordia affinis (RDA)、Myocoptes musculinus (COP)是當代最常見的三種實驗小鼠外寄生蟲。臺灣大學獸醫專業學院實驗動物醫學診斷研究室例行性健康監測中，發現一個疑似新興毛蟎感染病例。在這個研究，我們根據外觀資料(結構與特徵)與分子生物數據(核醣體核醣核酸ribosomal RNA [rRNA]與二級結構)以分析此新興毛蟎特徵與鑑定其物種分類，並將此小鼠毛蟎暫命名為Species A (SPA)。我們將三種小鼠毛蟎SPA、RDA和COP的18S與28S rRNA的基因序列與其他過去已經發佈的蟎蟲基因做數據比較分析，18S及28S rRNA基因的親緣分析顯示SPA與MOB及RDA應屬於同一個科(Myobiidae family)，而COP與SPA的親緣關係較遠。此外，28S rRNA的二級結構比較顯示結構雖然相似，但部分區域的結構多樣性建議未來可應用於類似SPA新興毛蟎物種的分辨。在SPA與其他小鼠蟎蟲的外觀特徵比較顯示SPA與MOB在外觀上難以辨別，與RDA的體型大小相似，但微細結構特徵仍不同，而與COP外表形狀有明顯差異，因此根據外觀分析建議SPA是Myobia屬的新成員。這篇研究的結論支持SPA與MOB相近，且SPA是Myobiidae科Myobia屬的新發現小鼠毛蟎。 | zh_TW |
| dc.description.abstract | Myobia murismusculi (aka Myobia musculi, MOB), Radfordia affinis (RDA) and Myocoptes musculinus (COP) are the three most prevalent ectoparasite species in contemporary laboratory mice. In this study, based on the morphological and molecular evidences and the predicted secondary structures of ribosomal RNA (rRNA), we have identified and characterized a novel fur mite from naturally infested laboratory mice and designated as Species A (SPA). The nucleotide sequences of the 18S and/or 28S rRNA genes of SPA, RDA, and COP were determined and compared with other previously characterized mites. Phylogenetic analysis results of the 18S and/or 28S rRNA gene sequences revealed that SPA belongs to the same family as MOBs and RDAs, and COP is least related to SPA. Genetic distance results indicated that SPA is most closely related to MOBs and RDAs, but distinctly different from COP. The comparison of the predicted secondary structures of 28S rRNA revealed that structural variations could be possibly applied in differentiating SPA-like organisms from other fur mites in the future. Comparison of morphological characters of SPA and other mouse fur mites showed that SPA is externally indistinguishable from MOB, with similar shape/size but different external structures to RDA, and highly distinct to COP in shape and externals. SPA was a novel member of Myobia genus. In conclusion, SPA was a novel member of Myobia genus in the family Myobiidae with MOB. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2023-09-07T16:55:09Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2023-09-07T16:55:09Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 口試委員會審定書 #
致謝 i Abstract in Chinese iii Abstract in English iv Contents v List of Figures vii List of Tables viii Introduction 1 Methods and Materials 6 Animal and fur mite samples 6 DNA extraction 6 Oligonucleotide primers 6 DNA amplification 7 DNA sequencing analysis and secondary structure prediction of 18S rRNA/28S rRNA gene 8 Morphological identification of fur mites 9 Results 11 Identification of non-MOB/RDA/COP fur mites in mice 11 DNA sequence analysis 12 Ribosomal RNA secondary structure prediction 13 The morphology of SPA, RDA-NTU, and COP-NTU 14 SPA 14 RDA‒NTU 15 COP‒NTU 15 Discussion 17 References 21 Appendix 1 External characters of SPA 45 Appendix 2 External characters of RDA-NTU 49 Appendix 3 External characters of COP-NTU 52 | - |
| dc.language.iso | en | - |
| dc.subject | 小鼠毛蟎 | zh_TW |
| dc.subject | 特徵化 | zh_TW |
| dc.subject | 外觀 | zh_TW |
| dc.subject | 分子生物 | zh_TW |
| dc.subject | 新興小鼠毛蟎 | zh_TW |
| dc.subject | novel mouse fur mite | en |
| dc.subject | characterization | en |
| dc.subject | morphology | en |
| dc.subject | mouse fur mite | en |
| dc.subject | molecular biology | en |
| dc.title | 新興小鼠毛蟎的外觀形態與分子特徵 | zh_TW |
| dc.title | Morphological and Molecular Characterization of a Novel Mouse Fur Mite | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 111-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 廖治榮;蕭超隆;謝淑貞;廖欽峯 | zh_TW |
| dc.contributor.oralexamcommittee | Jhih-Rong Liao;Chiaolong Hsiao;Shu-Chen Hsieh;Ching-Fong Liao | en |
| dc.subject.keyword | 小鼠毛蟎,新興小鼠毛蟎,特徵化,外觀,分子生物, | zh_TW |
| dc.subject.keyword | mouse fur mite,novel mouse fur mite,characterization,morphology,molecular biology, | en |
| dc.relation.page | 54 | - |
| dc.identifier.doi | 10.6342/NTU202301649 | - |
| dc.rights.note | 同意授權(全球公開) | - |
| dc.date.accepted | 2023-07-18 | - |
| dc.contributor.author-college | 生物資源暨農學院 | - |
| dc.contributor.author-dept | 分子暨比較病理生物學研究所 | - |
| dc.date.embargo-lift | 2025-07-17 | - |
| 顯示於系所單位： | 分子暨比較病理生物學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-111-2.pdf | 2.56 MB | Adobe PDF | 檢視/開啟 |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。