請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/8892
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 許瑞祥(Ruey-Shyang Hseu) | |
dc.contributor.author | Chiau-Ling Gung | en |
dc.contributor.author | 龔巧鈴 | zh_TW |
dc.date.accessioned | 2021-05-20T20:03:30Z | - |
dc.date.available | 2013-08-22 | |
dc.date.available | 2021-05-20T20:03:30Z | - |
dc.date.copyright | 2011-08-22 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-08-15 | |
dc.identifier.citation | 1. Awadh, A., R. Mothana, A. Lesnau, H. Pilgrim, and U. Lindequist. 2003. Antiviral activity of extracts and compounds from Inonotus hispidus. Fitotherapia 74: 483-5.
2. Ballario, P., P. Vittorioso, A. Magrelli, C. Talora, A. Cabibbo, and G. Macino. 1996. White collar-1, a central regulator of blue light responses in Neurospora, is a zinc finger protein. The EMBO Journal 15: 1650-1657. 3. Bender, S., C.N. Dumitrache-Anghel, J. Backhaus, G. Christie, R.F. Cross, G.T. Lonergan, and W.L. Baker. 2003. A Case for Caution in Assessing the Antibiotic Activity of Extracts of Culinary-Medicinal Shiitake Mushroom [ Lentinus edodes (Berk.) Singer] (Agaricomycetideae). Int. J. Med. Mushrooms 5: 31-5. 4. Bieszke, J.A., L. Li, and K.A. Borkovich. 2007. The fungal opsin gene nop-1 is negatively-regulated by a component of the blue light sensing pathway and influences conidiation-specific gene expression in Neurospora crassa. Curr. Genet. 52: 149-57. 5. Bisen, P., R. Baghel, B. Sanodiya, G. Thakur, and G. Prasad. 2010. Lentinus edodes: a macrofungus with pharmacological activities. Curr. Med. Chem. 17: 2419-2430. 6. Bobek, P. and Galbavy. 1999. Hypocholesterolemic and antiatherogenic effect of oyster mushroom (Pleurotus ostreatus) in rabbits. Nahrung 43: 339-342. 7. Bobek, P. and S. Galbavy. 2001. Effect of pleuran (beta-glucan from Pleurotus ostreatus) on the antioxidant status of the organism and on dimethylhydrazine-induced precancerous lesions in rat colon. Br. J. Biomed. Sci. 58: 164-8. 8. Brandt, C.R. and F. Piraino. 2000. Mushroom antivirals. Recent Res. Dev. Antimicrob. Agents Chemother. 4: 11-26. 9. Chen, C.H., B.S. Demay, A.S. Gladfelter, J.C. Dunlap, and J.J. Loros. 2010. Physical interaction between VIVID and white collar complex regulates photoadaptation in Neurospora. Proc. Natl. Acad. Sci. U. S. A.16715-16720. 10. Cheng, P., Y. Yang, K.H. Gardner, and Y. Liu. 2002. PAS domain-mediated WC-1/WC-2 interaction is essential for maintaining the steady-state level of WC-1 and the function of both proteins in circadian clock and light responses of Neurospora. Mol. Cell. Biol. 22: 517-24. 11. Chihara, G., Y. Maeda, J. Hamuro, T. Sasaki, and F. Fukuoka. 1969. Inhibition of mouse sarcoma 180 by polysaccharides from Lentinus edodes (Berk.) sing. Nature 222: 687-8. 12. Combs, G.F. and W.P. Gray. 1998. Chemopreventive agents selenium. Pharmacol. Ther. 79: 179-192. 13. Corrochano, L.M. 2007. Fungal photoreceptors: sensory molecules for fungal development and behaviour. Photochem. Photobiol. Sci. 6: 725-736. 14. Crosson, S., S. Rajagopal, and K. Moffat. 2003. The LOV domain family: photoresponsive signaling modules coupled to diverse output domains. Biochemistry (Mosc). 42: 2-10. 15. Darwin, C., ed. The power of movement in plants. 1881. 16. Ding, Y., S.V. Seow, C.H. Huang, L.M. Liew, Y.C. Lim, I.C. Kuo, and K.Y. Chua. 2009. Coadministration of the fungal immunomodulatory protein FIP-Fve and a tumour-associated antigen enhanced antitumour immunity. Immunology 128: e881-e894. 17. Durand, R. and R. Jacques. 1982. Action spectra for fruiting of the mushroom Coprinus congregatus. Arch. Microbiol. 132: 131-134. 18. El-Mekkawy, S., M.R. Meselhy, N. Nakamura, Y. Tezuka, M. Hattori, N. Kakiuchi, K. Shimotohno, T. Kawahata, and T. Otake. 1998. Anti-HIV-1 and anti-HIV-1-protease substances from Ganoderma lucidum. Phytochemistry 49: 1651-7. 19. Froehlich, A.C., B. Noh, R.D. Vierstra, J. Loros, and J.C. Dunlap. 2005. Genetic and molecular analysis of phytochromes from the filamentous fungus Neurospora crassa. Eukaryot. Cell 4: 2140-52. 20. Gao, Y., J. Lan, X. Dai, J. Ye, and S. Zhou. 2004. A Phase I/II Study of Ling Zhi Mushroom Ganoderma lucidum (W. Curt.: Fr.) Lloyd (Aphyllophoromycetideae) Extract in Patients with Type II Diabetes Mellitus. Int. J. Med. Mushrooms 6: 207-14. 21. Hawksworth, D.L. 2001. Mushrooms: the extent of the unexplored potential. Int. J. Med. Mushrooms 3: 333-7. 22. Hobbs, C., ed. Medicinal Mushrooms. . 1995, Santa Cruz: Botanica Press. 23. Hunt, S.M., S. Thompson, M. Elvin, and C. Heintzen. 2010. VIVID interacts with the WHITE COLLAR complex and FREQUENCY-interacting RNA helicase to alter light and clock responses in Neurospora. Proc. Natl. Acad. Sci. U. S. A. 107: 16709-16714. 24. Jose, N., T. Ajith, and K.K. Janardhanan. 2002. Antioxidant, Anti-inflammatory, and Antitumor Activities of Culinary-Medicinal Mushroom Pleurotus pufmonanus (Fr.) Quel. (Agaricomycetideae). Int. J. Med. Mushrooms 4: 329-35. 25. Kamada, T. 2002. Molecular genetics of sexual development in the mushroom Coprinus cinereus. Bioessays 24: 449-459. 26. Kamada, T., R. Kurita, and T. Takemaru. 1978. Effects of light on basidiocarp maturation in Coprinus macrorhizus. Plant Cell Physiol. 19: 263-275. 27. Kamada, T., H. Sano, T. Nakazawa, and K. Nakahori. 2010. Regulation of fruiting body photomorphogenesis in Coprinopsis cinerea. Fungal Genet. Biol. 47: 917-921. 28. Kohda, H., W. Tokumoto, K. Sakamoto, M. Fujii, Y. Hirai, K. Yamasaki, Y. Komoda, H. Nakamura, S. Ishihara, and M. Uchida. 1985. The biologically active constituents of Ganoderma lucidum (Fr.) Karst. Histamine release-inhibitory triterpenes. Chem. Pharm. Bull. 33: 1367-1374. 29. Komoda, Y., M. Shimizu, Y. Sonoda, and Y. Sato. 1989. Ganoderic acid and its derivatives as cholesterol synthesis inhibitors. Chem. Pharm. Bull. 37: 531-3. 30. Kues, U. 2000. Life history and developmental processes in the basidiomycete Coprinus cinereus. Microbiol. Mol. Biol. Rev. 64: 316-53. 31. Kuratani, M., K. Tanaka, K. Terashima, H. Muraguchi, T. Nakazawa, K. Nakahori, and T. Kamada. 2010. The dst2 gene essential for photomorphogenesis of Coprinopsis cinerea encodes a protein with a putative FAD-binding-4 domain. Fungal Genet. Biol. 47: 152-8. 32. Lavi, I., D. Friesem, S. Geresh, Y. Hadar, and B. Schwartz. 2006. An aqueous polysaccharide extract from the edible mushroom Pleurotus ostreatus induces anti-proliferative and pro-apoptotic effects on HT-29 colon cancer cells. Cancer Lett. 244: 61-70. 33. Lindequist, U., T.H.J. Niedermeyer, and W.D. Julich. 2005. The pharmacological potential of mushrooms. Evid. Based Complement. Alternat. Med. 2: 285-300. 34. Liu, F., V. Ooi, and S. Chang. 1997. Free radical scavenging activities of mushroom polysaccharide extracts. Life Sci. 60: 763-771. 35. Lu, Y.K., K.H. Sun, and W.C. Shen. 2005. Blue light negatively regulates the sexual filamentation via the Cwc1 and Cwc2 proteins in Cryptococcus neoformans. Mol. Microbiol. 56: 480-91. 36. Manzi, P., L. Gambelli, S. Marconi, V. Vivanti, and L. Pizzoferrato. 1999. Nutrients in edible mushrooms: an inter-species comparative study. Food Chem. 65: 477-482. 37. Martinez, D., J. Challacombe, I. Morgenstern, D. Hibbett, M. Schmoll, C.P. Kubicek, P. Ferreira, F.J. Ruiz-Duenas, A.T. Martinez, P. Kersten, K.E. Hammel, A. Vanden Wymelenberg, J. Gaskell, E. Lindquist, G. Sabat, S.S. Bondurant, L.F. Larrondo, P. Canessa, R. Vicuna, J. Yadav, H. Doddapaneni, V. Subramanian, A.G. Pisabarro, J.L. Lavin, J.A. Oguiza, E. Master, B. Henrissat, P.M. Coutinho, P. Harris, J.K. Magnuson, S.E. Baker, K. Bruno, W. Kenealy, P.J. Hoegger, U. Kues, P. Ramaiya, S. Lucas, A. Salamov, H. Shapiro, H. Tu, C.L. Chee, M. Misra, G. Xie, S. Teter, D. Yaver, T. James, M. Mokrejs, M. Pospisek, I.V. Grigoriev, T. Brettin, D. Rokhsar, R. Berka, and D. Cullen. 2009. Genome, transcriptome, and secretome analysis of wood decay fungus Postia placenta supports unique mechanisms of lignocellulose conversion. Proc. Natl. Acad. Sci. U. S. A. 106: 1954-9. 38. Mentel, R., D. Meinsen, H. Pilgrim, B. Herrmann, and U. Lindequist. 1994. In vitro antiviral effect of extracts of Kuehneromyces mutabilis on influenza virus. Pharmazie 49: 859-860. 39. Mizuno, T. 1999. The extraction and development of antitumor-active polysaccharides from medicinal mushrooms in Japan. Int. J. Med. Mushrooms 1: 9-29. 40. Molitoris, H.P. 1994. Mushrooms in medicine. Folia Microbiol. (Praha). 39: 91-98. 41. Mooney, J. and L. Yager. 1990. Light is required for conidiation in Aspergillus nidulans. Genes Dev. 4: 1473-1482. 42. Mothana, R.A.A., R. Jansen, W.D. Julich, and U. Lindequist. 2000. Ganomycins A and B, new antimicrobial farnesyl hydroquinones from the basidiomycete Ganoderma pfeifferi. J. Nat. Prod. 63: 416-418. 43. Purschwitz, J., S. Muller, C. Kastner, and R. Fischer. 2006. Seeing the rainbow: light sensing in fungi. Curr. Opin. Microbiol. 9: 566-71. 44. Rao, P. and D. Niederpruem. 1969. Carbohydrate metabolism during morphogenesis of Coprinus lagopus (sensu Buller). J. Bacteriol. 100: 1222-1228. 45. Reshetnikov, S.V. and K.K. Tan. 2001. Higher Basidiomycota as a Source of Antitumor and Immunostimulating Polysaccharides (Review). Int. J. Med. Mushrooms 3: 361-94. 46. Sabo, A., N. Stilinovic, S. Vukmirovic, Z. Bukumiric, I. Capo, and V. Jakovljevic. 2010. Pharmacodynamic action of a commercial preparation of the mushroom Coprinus comatus in rats. Abdom. Imaging 24: 1532-7. 47. Sano, H., S. Kaneko, Y. Sakamoto, T. Sato, and K. Shishido. 2009. The basidiomycetous mushroom Lentinula edodes white collar-2 homolog PHRB, a partner of putative blue-light photoreceptor PHRA, binds to a specific site in the promoter region of the L. edodes tyrosinase gene. Fungal Genet. Biol. 46: 333-41. 48. Sano, H., T. Narikiyo, S. Kaneko, T. Yamazaki, and K. Shishido. 2007. Sequence analysis and expression of a blue-light photoreceptor gene, Le.phrA from the basidiomycetous mushroom Lentinula edodes. Biosci. Biotechnol. Biochem. 71: 2206-13. 49. Smania, A., F. Monache, E. Smania, and R. Cuneo. 1999. Antibacterial activity of steroidal compounds isolated from Ganoderma applanatum (Pers.) Pat.(Aphyllophoromycetideae) fruit body. Int. J. Med. Mushrooms 1: 325-330. 50. Smania, E., F. Delle Monache, and A. Smania. 2003. Antifungal activity of sterols and triterpenes isolated from Ganoderma annulare. Fitoterapia 74: 375-377. 51. Starostzik, C. and W. Marwan. 1995. A photoreceptor with characteristics of phytochrome triggers sporulation in the true slime mould Physarum polycephalum. FEBS Lett. 370: 146-8. 52. Steinbrenner, H., E. Bilgic, L. Alili, H. Sies, and P. Brenneisen. 2006. Selenoprotein P protects endothelial cells from oxidative damage by stimulation of glutathione peroxidase expression and activity. Free Radic. Res. 40: 936-43. 53. Tasaka, K., M. Mio, K. Izushi, M. Akagi, and T. Makino. 1988. Anti-allergic constituents in the culture medium of Ganoderma lucidum.(II) The inhibitory effect of cyclooctasulfur on histamine release. Inflamm. Res. 23: 157-160. 54. Taylor, B. and I. Zhulin. 1999. PAS domains: internal sensors of oxygen, redox potential, and light. Microbiol. Mol. Biol. Rev. 63: 479-506. 55. Terashima, K., K. Yuki, H. Muraguchi, M. Akiyama, and T. Kamada. 2005. The dst1 gene involved in mushroom photomorphogenesis of Coprinus cinereus encodes a putative photoreceptor for blue light. Genetics 171: 101-8. 56. Wang, H., J. Gao, and T.B. Ng. 2000. A new lectin with highly potent antihepatoma and antisarcoma activities from the oyster mushroom Pleurotus ostreatus. Biochem. Biophys. Res. Commun. 275: 810-6. 57. Wasser, S.P., E. Nevo, D. Sokolov, S. Reshetnikov, and M. Timor-Tismenetsky. 2000. Dietary supplements from medicinal mushrooms: diversity of types and variety of regulations. Int. J. Med. Mushrooms 2: 1-19. 58. Wasser, S.P. and A.L. Weis. 1999. Medicinal properties of substances occurring in higher Basidiomycetes mushrooms: current perspectives. Int. J. Med. Mushrooms 1: 31-62. 59. Zjawiony, J.K. 2004. Biologically Active Compounds from Aphyllophorales (Polypore) Fungi. J. Nat. Prod. 67: 300-310. 60. 范嫻蘄. 2007. 以甘油醛-3-磷酸脫氫酶基因啟動子研究美白菇轉形系統. 國立台灣大學微生物與生化學研究所碩士論文. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/8892 | - |
dc.description.abstract | 在許多食藥用擔子菌的栽培過程中,要使其形成子實體必須控制多項環境因子,其中光線是影響其子實體發育是否完全的一個重要因素,顯示擔子菌中應有接受光線刺激的受器存在。目前研究顯示灰蓋鬼傘(Corprinus cinereus)、隱球菌(Cryptococcus neoformans)、香菇(Lentinula edodes)與褐腐菌(Postia placenta)等四種擔子菌具有與子囊菌紅麵包黴(Neurospora crassa)藍光受器White Collar-1 (WC-1)同源的蛋白質。本研究從常見食用真菌香菇、金針菇、鮑魚菇、美白菇與雞腿菇中選殖其WC-1同源藍光受器。目前使用專一性引子於香菇的互補DNA (complementary DNA, cDNA)中選殖到兩條與已發表之香菇藍光受器基因LephrA (accession no. AB279630)和LephrB (accession no. AB446463)相似之基因,分別命名為photoreceptor A與photoreceptor B。photoreceptor A全長為2,831 bp,其中包含了長56 bp的intron,與LephrA核酸與胺基酸相同度分別為99.5%和99.2%。photoreceptor B全長為942 bp,與LephrB核酸與胺基酸相同度為99.5%和99.6%。在基因組中,photoreceptor B被四個大小在52~87 bp之間intron分開。使用pET-21a(+)載體於大腸桿菌BL 21 (DE3)異源表現的Photoreceptor B-His x6為水溶性且大小約為37.7 kDa。同時以特定之退化性引子於金針菇cDNA中擴增出一段長420 bp的核酸序列,轉譯的140個胺基酸序列分別與香菇、裂褶菌(Schizophyllum commune)、布拉克鬍鬚黴(Phycomyces blakesleeanus)與灰蓋鬼傘中已發表的WC-1同源藍光受器分別具28%、28%、24%與31%相同度。 | zh_TW |
dc.description.abstract | For the fruiting-body formation, there are different environmental factors that must be controlled on the edible and medical mushroom cultivation. Light is one of the most important elements influencing the fruiting-body development, which indicates that there exists photoreceptor for receiving the light stimulus in the bacidiomycetes. The bacidiomycete Corprinus cinereus, Cryptococcus neoformans, Lentinula edodes and Postia placenta have the blue-light receptors that are homologs of White Collar-1 (WC-1) in ascomycete Neurospora crassa. In this study, we attempted to clone the WC-1 homologs from the edible mushrooms: L. edodes , Flammulina velutipes, Pleurotus ostreatus, Hypsizygus marmoreus variant and Coprinus comatus. We cloned two blue-light receptor genes, photoreceptor A and photoreceptor B, from L. edodes by using specific primers and the complementary DNA template of L. edodes for PCR. The genes photoreceptor A and photoreceptor B are similar to the published blue-light receptor genes LephrA (accession no. AB279630) and LephrB (accession no. AB279630) in L. edodes. The sequence of photoreceptor A is 2,831 bp and contains intron in 56 bp. The identity of nucleotides and amino acids between photoreceptor A and LephrA are 99.5% and 99.6%. The sequence of photoreceptor B is 942 bp and the identity of nucleotides and amino acids compared to LephrB are 99.5% and 99.6%. The photoreceptor B gene is divided by four introns in the genome and the size of introns is between 52 bp and 87 bp. The photoreceptor B gene were subcloned to pET-21a(+) and expressed in Escherichia coli BL 21 (DE3). The protein product Photoreceptor B-His x6 is soluble and the molecular weight is about 37.7 kDa. In the same time, we cloned a 420 bp nucleotide from the complementary DNA of F. velutipes by using the degenerate primers. The deduced 140 amino acids of the cloned nucleotide has 28%, 28%, 24% and 31% identity to the WC-1 homologs of L. edodes, Schizophyllum commune, Phycomyces blakesleeanus and C. cinereus. | en |
dc.description.provenance | Made available in DSpace on 2021-05-20T20:03:30Z (GMT). No. of bitstreams: 1 ntu-100-R98b47413-1.pdf: 6407916 bytes, checksum: 3926446170e3947a75bfd6d653fe731c (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 中文摘要 I
Abstract II 目錄 III 圖目錄 V 表目錄 VIII 第一章、前言 1 一、 食用菇類簡介 1 1. 菇類概述 1 2. 食用菇類人工栽培簡介 3 3. 常見食用菇類介紹 5 二、 真菌的光感知系統 7 1. 藍光感知系統 7 2. 紅光感知系統 8 3. 視紫質感知系統 8 三、 真菌藍光系統之研究情形 9 1. 紅麵包黴的藍光受器蛋白質 9 2. 保守性區域PAS 與LOV domain 10 3. 擔子菌門中藍光受器蛋白質之研究 10 四、 研究動機與目的 14 第二章 材料與方法 16 一、 實驗材料 16 1. 菌株及培養條件 16 2. 基因保存與表現系統 17 3. 使用質體 17 4. 實驗引子 18 二、 實驗方法 18 1. 菇類藍光受器基因釣取 18 2. 使用大腸桿菌生產photoreceptor B-His x6 32 第三章 實驗結果 37 一、 菇類藍光受器基因選殖 37 1. 嘗試由菇類基因體中以專一性引子擴增出藍光受器部分片段 37 2. 嘗試由菇類基因體中以退化性引子擴增出藍光受器部分片段 51 3. 嘗試由菇類cDNA中以退化性引子擴增出藍光受器部分片段 55 第四章、討論 67 一、 基因組中藍光受器的選殖 67 1. 專一性引子 67 2. 退化性引子 67 二、 香菇藍光受器基因的選殖 68 1. 本研究之LephrA :photorecetpr A的選殖 68 2. Photoreceptor-B-His x6處理菌絲結果 69 3. 金針菇中部分WC-1基因選殖 69 第五章、結論 72 第六章、未來工作 73 第七章、參考文獻 74 附錄 78 | |
dc.language.iso | zh-TW | |
dc.title | 擔子菌菇類藍光受器基因之選殖、序列分析與表現 | zh_TW |
dc.title | Cloning, sequence analysis and expression of the blue-light receptor gene from the basidiomycetous mushrooms | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 楊啟伸(Chii-Shen Yang),黃慶璨(Ching-Tsan Huang) | |
dc.subject.keyword | 擔子菌菇類,感光系統,藍光受器,香菇,金針菇, | zh_TW |
dc.subject.keyword | basidiomycetous mushroom,light-sensing system,blue-light receptor,Lentinula edodes,Flammuliana velutipes, | en |
dc.relation.page | 87 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2011-08-16 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科技學系 | zh_TW |
顯示於系所單位: | 生化科技學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-100-1.pdf | 6.26 MB | Adobe PDF | 檢視/開啟 |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。