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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 王兆麟 | zh_TW |
dc.contributor.advisor | Jaw-Lin Wang | en |
dc.contributor.author | 林威廷 | zh_TW |
dc.contributor.author | Wei-Ting Lin | en |
dc.date.accessioned | 2023-08-15T16:12:15Z | - |
dc.date.available | 2023-11-09 | - |
dc.date.copyright | 2023-08-15 | - |
dc.date.issued | 2023 | - |
dc.date.submitted | 2023-07-27 | - |
dc.identifier.citation | Susan C Kandarian and Robert W Jackman. Intracellular signaling during skeletal muscle atrophy. Muscle & Nerve: Official Journal of the American Association of Electrodiagnostic Medicine, 33(2):155–165, 2006.
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ZiMian Wang, Zhiliang Ying, Anja Bosy-Westphal, Junyi Zhang, Britta Schautz, Wiebke Later, Steven B Heymsfield, and Manfred J Müller. Specific metabolic rates of major organs and tissues across adulthood: evaluation by mechanistic model of resting energy expenditure. The American journal of clinical nutrition, 92(6):1369– 1377, 2010. Antonio Musarò. The basis of muscle regeneration. Advances in Biology, 2014, 2014. Jennifer Glaser and Masatoshi Suzuki. Skeletal muscle fiber types in neuromuscular diseases. Muscle Cell and Tissue-Current Status of Research Field, 2018. Anton Fomenko, Clemens Neudorfer, Robert F Dallapiazza, Suneil K Kalia, and Andres M Lozano. Low-intensity ultrasound neuromodulation: an overview of mecha- nisms and emerging human applications. Brain stimulation, 11(6):1209–1217, 2018. Thomas R Nelson, J Brian Fowlkes, Jacques S Abramowicz, and Charles C Church. Ultrasound biosafety considerations for the practicing sonographer and sonologist. 2009. Renee L Bunde, Eric J Jarvi, and Jeffrey J Rosentreter. Piezoelectric quartz crystal biosensors. Talanta, 46(6):1223–1236, 1998. Mark R Deakin and Daniel A Buttry. Electrochemical applications of the quartz crystal microbalance. Analytical Chemistry, 61(20):1147A–1154A, 1989. Susie Maestre. What is piezoelectric effect? https://www.circuitbread.com/ee-faq/ what-is-piezoelectric-effect, 2022. Lotte B Pedersen, Iben R Veland, Jacob M Schrøder, and Søren T Christensen. As- sembly of primary cilia. Developmental dynamics: an official publication of the American Association of Anatomists, 237(8):1993–2006, 2008. Kousuke Kasahara and Masaki Inagaki. Primary ciliary signaling: Links with the cell cycle. Trends in Cell Biology, 31(12):954–964, 2021. Westley Heydeck, Lorraine Fievet, Erica E Davis, and Nicholas Katsanis. The com- plexity of the cilium: spatiotemporal diversity of an ancient organelle. Current opin- ion in cell biology, 55:139–149, 2018. 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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/88417 | - |
dc.description.abstract | 低能量超音波已被證實能夠促進C2C12的增生與分化。壓電效應則被證實能透過初級纖毛來重新排列軟骨細胞,且初級纖毛在骨骼肌肉分化中扮演重要的角色。然而,超音波與壓電對於治療肌肉萎縮的應用仍未明朗,故本研究旨在探討超音波與壓電刺激在治療肌肉萎縮方面的可行性。
我們選擇C2C12作為體外模型,進行了多次超音波刺激。結果顯示,超音波明顯促進了C2C12的融合程度。與未刺激組相比,經超音波刺激後的C2C12在分化第二天時的MyHC3表現量有增加的趨勢,這表明超音波刺激可能增強了C2C12的分化能力。 為了研究壓電對C2C12的影響,我們給與石英玻片超音波以產生電場。培養有C2C12的玻片放置於石英玻片上以進行壓電刺激。結果顯示,壓電導致C2C12細胞核的形態變化,細胞核變小且細長,同時初級纖毛的組裝也得到提升,特別是在刺激後12小時更明顯。我們推測壓電可能通過在早期分化階段促進初級纖毛的組裝,加速C2C12的分化。 在動物實驗中,我們切斷右腿脛神經來建立小鼠肌肉萎縮模型。自第二週開始,我們對小鼠進行了為期三週,每週三次、每次五分鐘的聚焦超音波和壓電治療。研究結果顯示,聚焦超音波和壓電治療明顯抑制了小鼠肌纖維橫截面積的下降,尤其聚焦超音波治療進一步顯著抑制了腓腸肌的重量下降。本研究證實超音波治療改善肌肉萎縮的可能性,並顯示超音波在治療肌肉退化方面具有潛力。 | zh_TW |
dc.description.abstract | Low-intensity ultrasound has been proven to enhance the proliferation and differentiation of C2C12 cells. The piezoelectric effect has also been demonstrated to reorganize chondrocytes through primary cilia, which play a crucial role in skeletal muscle differentiation. However, the application of ultrasound and piezoelectric stimulation for treating muscle atrophy remains uncertain. Therefore, this study aims to investigate the feasibility of utilizing ultrasound and piezoelectric stimulation for the treatment of muscle atrophy.
We selected C2C12 as an in vitro model and conducted multiple rounds of ultrasound stimulation. The results clearly indicate that ultrasound significantly promotes the fusion of C2C12 cells. Compared to the unstimulated group, the C2C12 cells stimulated with ultrasound showed a noticeable increase in MyHC3 expression on the second day of differentiation, suggesting that ultrasound stimulation might enhance the differentiation of C2C12 cells. To explore the impact of piezoelectric stimulation on C2C12 cells, we applied ultrasound to quartz slides to generate an electric field. Quartz slides containing C2C12 cells were placed on the quartz slides to undergo piezoelectric stimulation. The outcomes revealed that piezoelectric stimulation induced morphological alterations in the nuclei of C2C12 cells, resulting in smaller and elongated nuclei. Furthermore, there was an enhanced assembly of primary cilia, particularly evident at 12 hours after stimulation. It is hypothesized that piezoelectric stimulation may promote the assembly of primary cilia during the early stages of differentiation, thereby accelerating the differentiation process of C2C12 cells. In animal study, we established a mouse model of muscle atrophy by transecting the tibial nerve. Starting from the second week, the mice underwent three weeks of focused ultrasound and piezoelectric treatment, administered three times a week for five minutes each session. The findings demonstrated that focused ultrasound and piezoelectric treatment significantly suppressed the reduction in the cross-section area of mouse muscle fibers, with focused ultrasound treatment particularly demonstrating a remarkable inhibition of weight loss in the gastrocnemius muscle. This study confirms the potential of ultrasound treatment in ameliorating muscle atrophy and highlights the potential of ultrasound as a therapeutic modality for muscle degeneration. | en |
dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2023-08-15T16:12:15Z No. of bitstreams: 0 | en |
dc.description.provenance | Made available in DSpace on 2023-08-15T16:12:15Z (GMT). No. of bitstreams: 0 | en |
dc.description.tableofcontents | 口試委員會審定書 i
誌謝 ii 中文摘要 iv 英文摘要 v 發表著作 vii 目錄 viii 圖目錄 xii 表目錄 xv 第一章 緒論 1 1.1 研究背景 1 1.2 肌肉簡介 2 1.2.1 骨骼肌分化 2 1.2.2 C2C12 肌母細胞 3 1.3 超音波簡介 4 1.3.1 超音波參數 4 1.3.2 超音波強度計算 5 1.3.3 超音波與壓電效應 7 1.4 初級纖毛 8 1.5 前導實驗-C2C12 鈣離子調控實驗 9 1.6 實驗目的 11 第二章 材料與方法 12 2.1 C2C12 細胞培養 12 2.1.1 細胞繼代與種植 12 2.1.2 肌管分化 13 2.2 微圖案 (Micropattern) 14 2.3 小鼠肌肉萎縮模型建立 16 2.4 溫度量測 17 2.5 聲強度量測 17 2.6 超音波刺激裝置 18 2.6.1 訊號產生器 (Function generator) 18 2.6.2 功率放大器 (Power Amplifier) 19 2.6.3 超音波刺激裝置架設 19 2.6.4 聲強度量測結果 19 2.6.5 溫度量測結果 25 2.7 壓電刺激裝置 26 2.7.1 CelleX 訊號產生器 26 2.7.2 mini-LIC 26 2.7.3 壓電薄膜 (Piezoelectric film) 27 2.7.4 壓電刺激裝置架設 28 2.7.5 聲強度量測結果 28 2.7.6 電場模擬 31 2.7.7 溫度量測結果 31 2.7.8 mini-LIC 刺激裝置散熱設計 34 2.7.8.1 散熱溫度量測結果 34 2.8 生物檢測法 35 2.8.1 免疫螢光染色 (Immunofluorescence) 35 2.8.2 西方墨點法 (Western Blot) 37 2.9 肌管融合實驗 (US) 39 2.9.1 實驗設計 39 2.9.2 免疫螢光染色 40 2.10 分化程度實驗 (US) 41 2.10.1 實驗設計 41 2.10.2 西方墨點法 42 2.11 細胞排列與分佈實驗 (PE) 43 2.11.1 實驗設計 43 2.11.2 免疫螢光染色 43 2.12 小鼠肌肉萎縮治療實驗 (US&PE) 45 2.12.1 實驗設計 45 第三章 實驗結果與討論 47 3.1 肌管融合實驗 (US) 47 3.1.1 肌管融合比例分析 47 3.2 分化程度實驗 (US) 49 3.2.1 MyHC3 西方墨點法結果 49 3.3 細胞排列與分佈實驗 (PE) 50 3.3.1 細胞核分析 50 3.3.2 初級纖毛分析 52 3.4 小鼠肌肉萎縮治療實驗 (US&PE) 54 3.4.1 腓腸肌重量分析 54 3.4.2 肌纖維橫截面積分析 55 3.5 討論 56 3.5.1 肌管融合實驗與分化程度實驗 (US) 56 3.5.2 細胞排列與分佈實驗 (PE) 57 3.5.3 小鼠肌肉萎縮治療實驗 (US&PE) 58 第四章 結論與未來展望 59 4.1 結論 59 4.2 未來展望 59 參考文獻 60 | - |
dc.language.iso | zh_TW | - |
dc.title | 超音波對肌肉再生之探討 | zh_TW |
dc.title | Investigation of Ultrasound Stimulation on Muscle Regeneration | en |
dc.type | Thesis | - |
dc.date.schoolyear | 111-2 | - |
dc.description.degree | 碩士 | - |
dc.contributor.oralexamcommittee | 林錫賢;陳文翔;朱業修 | zh_TW |
dc.contributor.oralexamcommittee | Hsi-Hsien Lin ;Wen-Shiang Chen;Yeh-Shiu Chu | en |
dc.subject.keyword | 低能量超音波,壓電刺激,肌肉萎縮,C2C12肌母細胞,初級纖毛,分化, | zh_TW |
dc.subject.keyword | Low-intensity ultrasound,Piezoelectric stimulation,Muscle atrophy,C2C12 Myoblast,Primary cilia,Differentiation, | en |
dc.relation.page | 64 | - |
dc.identifier.doi | 10.6342/NTU202301034 | - |
dc.rights.note | 同意授權(全球公開) | - |
dc.date.accepted | 2023-07-31 | - |
dc.contributor.author-college | 工學院 | - |
dc.contributor.author-dept | 醫學工程學系 | - |
顯示於系所單位: | 醫學工程學研究所 |
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