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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳佩貞 | |
| dc.contributor.author | Chun-Hung Lin | en |
| dc.contributor.author | 林均鴻 | zh_TW |
| dc.date.accessioned | 2021-05-20T20:02:04Z | - |
| dc.date.available | 2014-08-20 | |
| dc.date.available | 2021-05-20T20:02:04Z | - |
| dc.date.copyright | 2011-08-20 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-08-16 | |
| dc.identifier.citation | Allen, J.W., D.C. Wolf, M.H. George, S.D. Hester, G. Sun, S.F. Thai, D.A. Delker, T. Moore, C. Jones, G. Nelson, B.C. Roop, S. Leavitt, E. Winkfield, W.O. Ward, and S. Nesnow. 2006. Toxicity profiles in mice treated with hepatotumorigenic and non-hepatotumorigenic triazole conazole fungicides: Propiconazole, triadimefon, and myclobutanil. Toxicol Pathol 34:853-62.
Beijer, K., A. Abrahamson, B. Brunstrom, and I. Brandt. 2010. CYP1A inhibition in fish gill filaments: a novel assay applied on pharmaceuticals and other chemicals. Aquat Toxicol 96:145-50. Berard, J., F. Laboune, M. Mukuna, S. Masse, R. Kothary, and W.E. Bradley. 1996. Lung tumors in mice expressing an antisense RARbeta2 transgene. FASEB J 10:1091-7. Berenzen, N., A. Lentzen-Godding, M. Probst, H. Schulz, R. Schulz, and M. Liess. 2005. A comparison of predicted and measured levels of runoff-related pesticide concentrations in small lowland streams on a landscape level. Chemosphere 58:683-91. Blomhoff, R., and H.K. Blomhoff. 2006. Overview of retinoid metabolism and function. J Neurobiol 66:606-30. Boelsterli, U.A., A. Lanzotti, C. Goldlin, and M. Oertle. 1992. Identification of cytochrome P-450IIB1 as a cocaine-bioactivating isoform in rat hepatic microsomes and in cultured rat hepatocytes. Drug Metab Dispos 20:96-101. Canestro, C., J.M. Catchen, A. Rodriguez-Mari, H. Yokoi, and J.H. Postlethwait. 2009. Consequences of lineage-specific gene loss on functional evolution of surviving paralogs: ALDH1A and retinoic acid signaling in vertebrate genomes. PLoS Genet 5:e1000496. Castillo, L., G. Milano, J. Santini, F. Demard, and V. Pierrefite. 1997. Analysis of retinoic acid receptor beta expression in normal and malignant laryngeal mucosa by a sensitive and routine applicable reverse transcription-polymerase chain reaction enzyme-linked immunosorbent assay method. Clin Cancer Res 3:2137-42. Chambon, P. 1996. A decade of molecular biology of retinoic acid receptors. FASEB J 10:940-54. Chen, H., W.N. Howald, and M.R. Juchau. 2000. Biosynthesis of all-trans-retinoic acid from all-trans-retinol: catalysis of all-trans-retinol oxidation by human P-450 cytochromes. Drug Metab Dispos 28:315-22. Chen, P.J., W.T. Padgett, T. Moore, W. Winnik, G.R. Lambert, S.F. Thai, S.D. Hester, and S. Nesnow. 2009. Three conazoles increase hepatic microsomal retinoic acid metabolism and decrease mouse hepatic retinoic acid levels in vivo. Toxicol Appl Pharmacol 234:143-55. Choi, J., and L.A. Donehower. 1999. p53 in embryonic development: maintaining a fine balance. Cell Mol Life Sci 55:38-47. Collins, M.D., and G.E. Mao. 1999. Teratology of retinoids. Annu Rev Pharmacol Toxicol 39:399-430. EPA. 1996. Triadimefon; Pesticide Tolerances for Emergency Exemptions. Fed Regist 61:63721–6. EPA. 2000. Myclobutanil; pesticide tolerances. Fed Regist 65:29963-73. EPA. 2006. Re-registration eligibility decision for triadimefon and triadimenol and tolerance reassessment for triadimenol. EPA. 2009. Risks of Myclobutanil Use to Federally Threatened California Red-legged Frog (Rana aurora draytonii) and Designated Critical Habitat. Gabelova, A., B. Binkova, Z. Valovicova, and R.J. Sram. 2004. DNA adduct formation by 7H-dibenzo[c,g]carbazole and its tissue- and organ-specific derivatives in Chinese hamster V79 cell lines stably expressing cytochrome P450 enzymes. Environ Mol Mutagen 44:448-58. Georgopapadakou, N.H., and T.J. Walsh. 1996. Antifungal agents: chemotherapeutic targets and immunologic strategies. Antimicrob Agents Chemother 40:279-91. Ghannoum, M.A., and L.B. Rice. 1999. Antifungal agents: mode of action, mechanisms of resistance, and correlation of these mechanisms with bacterial resistance. Clin Microbiol Rev 12:501-17. Goetz, A.K., and D.J. Dix. 2009. Mode of action for reproductive and hepatic toxicity inferred from a genomic study of triazole antifungals. Toxicol Sci 110:449-62. Goetz, A.K., W. Bao, H. Ren, J.E. Schmid, D.B. Tully, C. Wood, J.C. Rockett, M.G. Narotsky, G. Sun, G.R. Lambert, S.F. Thai, D.C. Wolf, S. Nesnow, and D.J. Dix. 2006. Gene expression profiling in the liver of CD-1 mice to characterize the hepatotoxicity of triazole fungicides. Toxicol Appl Pharmacol 215:274-84. Greenblatt, D.J., Y. Zhao, K. Venkatakrishnan, S.X. Duan, J.S. Harmatz, S.J. Parent, M.H. Court, and L.L. von Moltke. 2011. Mechanism of cytochrome P450-3A inhibition by ketoconazole. J Pharm Pharmacol 63:214-21. Guengerich, F.P. 2003. Cytochromes P450, drugs, and diseases. Mol Interv 3:194-204. Hasselberg, L., S. Westerberg, B. Wassmur, and M.C. Celander. 2008. Ketoconazole, an antifungal imidazole, increases the sensitivity of rainbow trout to 17alpha-ethynylestradiol exposure. Aquat Toxicol 86:256-64. Hester, S.D., D.C. Wolf, S. Nesnow, and S.F. Thai. 2006. Transcriptional profiles in liver from rats treated with tumorigenic and non-tumorigenic triazole conazole fungicides: Propiconazole, triadimefon, and myclobutanil. Toxicol Pathol 34:879-94. Ho, Y.S., P.W. Tsai, C.F. Yu, H.L. Liu, R.J. Chen, and J.K. Lin. 1998. Ketoconazole-induced apoptosis through P53-dependent pathway in human colorectal and hepatocellular carcinoma cell lines. Toxicol Appl Pharmacol 153:39-47. Hong, H.N., H.N. Kim, K.S. Park, S.K. Lee, and M.B. Gu. 2007. Analysis of the effects diclofenac has on Japanese medaka (Oryzias latipes) using real-time PCR. Chemosphere 67:2115-21. Honkakoski, P., and M. Negishi. 2000. Regulation of cytochrome P450 (CYP) genes by nuclear receptors. Biochem J 347:321-37. Houle, B., C. Rochette-Egly, and W.E. Bradley. 1993. Tumor-suppressive effect of the retinoic acid receptor beta in human epidermoid lung cancer cells. Proc Natl Acad Sci U S A 90:985-9. INCHEM. 1981. Monograph 566. Triadimefon Pesticide residues in food: 1981 evaluations. INCHEM. 1992. Monograph 847. Myclobutanil Pesticide residues in food: 1992 evaluations Part II Toxicology. Jackson, J.G., S.M. Post, and G. Lozano. 2011. Regulation of tissue- and stimulus-specific cell fate decisions by p53 in vivo. J Pathol 223:127-36. Kahle, M., I.J. Buerge, A. Hauser, M.D. Muller, and T. Poiger. 2008. Azole fungicides: occurrence and fate in wastewater and surface waters. Environ Sci Technol 42:7193-200. Kark, J.D., A.H. Smith, B.R. Switzer, and C.G. Hames. 1981. Serum vitamin A (retinol) and cancer incidence in Evans County, Georgia. J Natl Cancer Inst 66:7-16. Kashiwada, S., M. Kameshiro, H. Tatsuta, Y. Sugaya, S.W. Kullman, D.E. Hinton, and K. Goka. 2007. Estrogenic modulation of CYP3A38, CYP3A40, and CYP19 in mature male medaka (Oryzias latipes). Comp Biochem Physiol C Toxicol Pharmacol 145:370-8. Kassahun, K., P.G. Pearson, W. Tang, I. McIntosh, K. Leung, C. Elmore, D. Dean, R. Wang, G. Doss, and T.A. Baillie. 2001. Studies on the metabolism of troglitazone to reactive intermediates in vitro and in vivo. Evidence for novel biotransformation pathways involving quinone methide formation and thiazolidinedione ring scission. Chem Res Toxicol 14:62-70. Leguen, I., N. Odjo, Y. Le Bras, B. Luthringer, D. Baron, G. Monod, and P. Prunet. 2010. Effect of seawater transfer on CYP1A gene expression in rainbow trout gills. Comp Biochem Physiol A Mol Integr Physiol 156:211-7. Lewis, D.F. 2003. Human cytochromes P450 associated with the phase 1 metabolism of drugs and other xenobiotics: a compilation of substrates and inhibitors of the CYP1, CYP2 and CYP3 families. Curr Med Chem 10:1955-72. Lewis, D.F. 2004. 57 varieties: the human cytochromes P450. Pharmacogenomics 5:305-18. Lotan, R., X.C. Xu, S.M. Lippman, J.Y. Ro, J.S. Lee, J.J. Lee, and W.K. Hong. 1995. Suppression of retinoic acid receptor-beta in premalignant oral lesions and its up-regulation by isotretinoin. N Engl J Med 332:1405-10. Lotan, Y., X.C. Xu, M. Shalev, R. Lotan, R. Williams, T.M. Wheeler, T.C. Thompson, and D. Kadmon. 2000. Differential expression of nuclear retinoid receptors in normal and malignant prostates. J Clin Oncol 18:116-21. Ma, Q., and A.Y. Lu. 2007. CYP1A induction and human risk assessment: an evolving tale of in vitro and in vivo studies. Drug Metab Dispos 35:1009-16. Ma, T., Z. Wang, and S. Gong. 2007. Comparative sensitivity in Chinese rare minnow (Gobiocypris rarus) and Japanese Medaka (Oryzias latipes) exposed to ethinylestradiol. J Environ Sci Health A Tox Hazard Subst Environ Eng 42:889-94. Ma, T., X. Wan, Q. Huang, Z. Wang, and J. Liu. 2005. Biomarker responses and reproductive toxicity of the effluent from a Chinese large sewage treatment plant in Japanese medaka (Oryzias latipes). Chemosphere 59:281-8. Monostory, K., K. Jemnitz, and L. Vereczkey. 1996. Xenobiotic metabolizing enzymes in fish: diversity, regulation and biomarkers for pollutant exposure. Acta Physiol Hung 84:369-81. Morriss-Kay, G.M., and S.J. Ward. 1999. Retinoids and mammalian development. Int Rev Cytol 188:73-131. Nannelli, A., V. Chirulli, V. Longo, and P.G. Gervasi. 2008. Expression and induction by rifampicin of CAR- and PXR-regulated CYP2B and CYP3A in liver, kidney and airways of pig. Toxicology 252:105-12. Nebert, D.W., and D.W. Russell. 2002. Clinical importance of the cytochromes P450. Lancet 360:1155-62. Nesnow, S., W. Ward, T. Moore, H. Ren, and S.D. Hester. 2009. Discrimination of tumorigenic triazole conazoles from phenobarbital by transcriptional analyses of mouse liver gene expression. Toxicol Sci 110:68-83. Niles, R.M. 2000. Recent advances in the use of vitamin A (retinoids) in the prevention and treatment of cancer. Nutrition 16:1084-9. Picard, E., C. Seguin, N. Monhoven, C. Rochette-Egly, J. Siat, J. Borrelly, Y. Martinet, N. Martinet, and J.M. Vignaud. 1999. Expression of retinoid receptor genes and proteins in non-small-cell lung cancer. J Natl Cancer Inst 91:1059-66. Qiu, H., W. Zhang, A.K. El-Naggar, S.M. Lippman, P. Lin, R. Lotan, and X.C. Xu. 1999. Loss of retinoic acid receptor-beta expression is an early event during esophageal carcinogenesis. Am J Pathol 155:1519-23. Robertson, I.G., E. Zeiger, and J.A. Goldstein. 1983. Specificity of rat liver cytochrome P-450 isozymes in the mutagenic activation of benzo[a]pyrene, aromatic amines and aflatoxin B1. Carcinogenesis 4:93-6. Sakaki, T., N. Kagawa, K. Yamamoto, and K. Inouye. 2005. Metabolism of vitamin D3 by cytochromes P450. Front Biosci 10:119-34. Selkirk, J.K. 1977. Benzo[a]pyrene carcinogenesis: a biochemical selection mechanism. J Toxicol Environ Health 2:1245-58. Sheehan, D.J., C.A. Hitchcock, and C.M. Sibley. 1999. Current and emerging azole antifungal agents. Clin Microbiol Rev 12:40-79. Singh, N. 2005. Factors affecting triadimefon degradation in soils. J Agric Food Chem 53:70-5. Smolowitz, R.M., M.E. Schultz, and J.J. Stegeman. 1992. Cytochrome P4501A induction in tissues, including olfactory epithelium, of topminnows (Poeciliopsis spp.) by waterborne benzo[a]pyrene. Carcinogenesis 13:2395-402. Sommer, A. 2008. Vitamin a deficiency and clinical disease: an historical overview. J Nutr 138:1835-9. Sun, G., R.D. Grindstaff, S.F. Thai, G.R. Lambert, D.B. Tully, D.J. Dix, and S. Nesnow. 2007. Induction of cytochrome P450 enzymes in rat liver by two conazoles, myclobutanil and triadimefon. Xenobiotica 37:180-93. Sun, S.Y., and R. Lotan. 2002. Retinoids and their receptors in cancer development and chemoprevention. Crit Rev Oncol Hematol 41:41-55. Tatarazako, N., H. Takigami, M. Koshio, K. Kawabe, Y. Hayakawa, K. Arizono, and M. Morita. 2002. New Measurement Method of P450s Activities in the Liver Microsome with Individual Japanese Medaka(Oryzias latipes). Environ Sci 9:451-462. Thatcher, J.E., and N. Isoherranen. 2009. The role of CYP26 enzymes in retinoic acid clearance. Expert Opin Drug Metab Toxicol 5:875-86. Theodosiou, M., V. Laudet, and M. Schubert. 2010. From carrot to clinic: an overview of the retinoic acid signaling pathway. Cell Mol Life Sci 67:1423-45. Tompkins, L.M., and A.D. Wallace. 2007. Mechanisms of cytochrome P450 induction. J Biochem Mol Toxicol 21:176-81. Tully, D.B., W. Bao, A.K. Goetz, C.R. Blystone, H. Ren, J.E. Schmid, L.F. Strader, C.R. Wood, D.S. Best, M.G. Narotsky, D.C. Wolf, J.C. Rockett, and D.J. Dix. 2006. Gene expression profiling in liver and testis of rats to characterize the toxicity of triazole fungicides. Toxicol Appl Pharmacol 215:260-73. Ward, W.O., D.A. Delker, S.D. Hester, S.F. Thai, D.C. Wolf, J.W. Allen, and S. Nesnow. 2006. Transcriptional profiles in liver from mice treated with hepatotumorigenic and nonhepatotumorigenic triazole conazole fungicides: Propiconazole, triadimefon, and myclobutanil. Toxicol Pathol 34:863-78. Widschwendter, M., J. Berger, G. Daxenbichler, E. Muller-Holzner, A. Widschwendter, A. Mayr, C. Marth, and A.G. Zeimet. 1997. Loss of retinoic acid receptor beta expression in breast cancer and morphologically normal adjacent tissue but not in the normal breast tissue distant from the cancer. Cancer Res 57:4158-61. Williams, D.E., J.J. Lech, and D.R. Buhler. 1998. Xenobiotics and xenoestrogens in fish: modulation of cytochrome P450 and carcinogenesis. Mutat Res 399:179-92. Williams, J.A., R. Hyland, B.C. Jones, D.A. Smith, S. Hurst, T.C. Goosen, V. Peterkin, J.R. Koup, and S.E. Ball. 2004. Drug-drug interactions for UDP-glucuronosyltransferase substrates: a pharmacokinetic explanation for typically observed low exposure (AUCi/AUC) ratios. Drug Metab Dispos 32:1201-8. Wolbach, S.B., and P.R. Howe. 1925. Tissue Changes Following Deprivation of Fat-Soluble a Vitamin. J Exp Med 42:753-77. Xu, X.C., J.Y. Ro, J.S. Lee, D.M. Shin, W.K. Hong, and R. Lotan. 1994. Differential expression of nuclear retinoid receptors in normal, premalignant, and malignant head and neck tissues. Cancer Res 54:3580-7. Xu, X.C., G. Sozzi, J.S. Lee, J.J. Lee, U. Pastorino, S. Pilotti, J.M. Kurie, W.K. Hong, and R. Lotan. 1997a. Suppression of retinoic acid receptor beta in non-small-cell lung cancer in vivo: implications for lung cancer development. J Natl Cancer Inst 89:624-9. Xu, X.C., N. Sneige, X. Liu, R. Nandagiri, J.J. Lee, F. Lukmanji, G. Hortobagyi, S.M. Lippman, K. Dhingra, and R. Lotan. 1997b. Progressive decrease in nuclear retinoic acid receptor beta messenger RNA level during breast carcinogenesis. Cancer Res 57:4992-6. Yamauchi, R., H. Ishibashi, M. Hirano, T. Mori, J.W. Kim, and K. Arizono. 2008. Effects of synthetic polycyclic musks on estrogen receptor, vitellogenin, pregnane X receptor, and cytochrome P450 3A gene expression in the livers of male medaka (Oryzias latipes). Aquat Toxicol 90:261-8. Zarn, J.A., B.J. Bruschweiler, and J.R. Schlatter. 2003. Azole fungicides affect mammalian steroidogenesis by inhibiting sterol 14 alpha-demethylase and aromatase. Environ Health Perspect 111:255-61. Zhang, X., M. Hecker, A.R. Tompsett, J.W. Park, P.D. Jones, J. Newsted, D. Au, R. Kong, R.S. Wu, and J.P. Giesy. 2008a. Responses of the medaka HPG axis PCR array and reproduction to prochloraz and ketoconazole. Environ Sci Technol 42:6762-9. Zhang, Z., and J. Hu. 2008. Effects of p,p'-DDE exposure on gonadal development and gene expression in Japanese medaka (Oryzias latipes). J Environ Sci (China) 20:347-52. Zhang, Z., J. Hu, H. Zhen, X. Wu, and C. Huang. 2008b. Reproductive inhibition and transgenerational toxicity of triphenyltin on medaka (Oiyzias latipes) at environmentally relevant levels. Environ Sci Technol 42:8133-9. 陳冠雯. 2011. 康唑類殺菌劑和人造雌激素混合暴露干擾青鱂魚內分泌作用相關基因表達之研究, 臺灣大學. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/8825 | - |
| dc.description.abstract | 三泰芬 (Triadimefon) 及邁克尼 (Myclobutanil) 為農業上廣為使用的康唑類殺真菌劑 (Conazoles)。研究顯示三泰芬 (具致腫瘤性) 及邁克尼 (不具致腫瘤性) 會干擾小鼠肝中重要代謝酵素細胞色素P450 (Cytochrome P450, CYP) 基因表現及酵素活性，進而加劇維生素A活性形式全反式視黃酸 (All-trans retinoic acid, atRA) 之代謝。但三泰芬及邁克尼對水生生物之影響的相關研究仍有限，因此本研究探討三泰芬及邁克尼對青鱂魚 (Oryzias latipes, medaka) 肝中CYPs基因表現量、酵素活性及atRA相關基因表現之干擾作用。結果顯示，暴露三泰芬及邁克尼 (2 mg/L) 3天後青鱂魚肝中CYP3A38及CYP3A40之mRNA表現有提升趨勢。暴露三泰芬溶液 (2.0-3.5 μM) 7天後會提升CYP1A和CYP3A活性，而暴露邁克尼溶液 (2.0-3.5 μM) 則僅提升CYP3A活性。此外，合成atRA之酵素ALDH1A2之mRNA表現量不受實驗處理影響，而代謝內生atRA之酵素CYP26B1之mRNA表現量則受三泰芬 (2.0-3.5 μM) 誘導提升，顯示三泰芬造成水生生物中atRA過量代謝的可能性較邁克尼高。在atRA相關受體中，RARγ1受三泰芬 (2.0 μM) 誘導，顯示三泰芬可能經由RARγ1途徑誘導CYP26B1基因表現；而三泰芬及邁克尼 (2.0 μM) 暴露後RXRα1及RXRβ1之mRNA表現量皆下降，顯示三泰芬及邁克尼皆可能會影響細胞對atRA之感知能力，進而影響atRA參與調控之生理機制，但無法以此做為康唑類殺真菌劑是否具有致癌性之生物指標。綜合以上結果，暴露於三泰芬和邁克尼會提高青鱂魚肝臟中特定CYPs基因表現及活性，並干擾atRA相關核受體之mRNA表現，可能會進而影響維生素A之正常代謝及其參與之生理調控機制。 | zh_TW |
| dc.description.abstract | Triadimefon and myclobutanil are triazole-containing conazole fungicides widely used in agriculture. Previous studies show that some conazoles (e.g. triadimefon) at sublethal dose induced gene expression and enzymatic activity of cytochrome P450s (CYPs) in the liver of mouse, leading to a decrease in hepatic level of all-trans retinoic acid (atRA), an active form of vitamin A with anticancer properties. This study demonstrates the effects of carcinogenic triadimefon and non-carcinogenic myclobutanil on the activity of CYPs and mRNA expression of atRA-related genes in the liver of an aquatic organism, medaka (Oryzias latipes). Enzymatic activity analyses show triadimefon (2.0-3.5 μM) induced the activities of CYP1A and CYP3A, while myclobutanil (2.0-3.5 μM) only induced the activity of CYP3A. Quantitative real-time PCR analyses revealed that only triadimefon (2.0-5.0 μM) induced mRNA expression of endogenous atRA metabolism enzyme CYP26B1. Results from both enzymatic and genetic analyses above indicating that triadimefon may enhance hepatic atRA metabolism more severely than myclubutanil could do. Gene expression of atRA-related nuclear receptors retinoid X receptors (rxrα1 and rxrβ1) was depressed by both triadimefon and myclobutanil, indicating that both conazoles may interrupt the ability of cell to sense atRA and leading to disruption of atRA-regulated mechanisms. Overall, both triadimefon and myclobutanil interfere with the activity of cytochrome P450s and mRNA expression of all-trans retinoic acid-related genes in the liver of medaka. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-20T20:02:04Z (GMT). No. of bitstreams: 1 ntu-100-R98623019-1.pdf: 2577235 bytes, checksum: bebd9c7d97d5da8d374e346ce8463d9a (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 摘要 I
Abstract II 目錄 III 圖目錄 VI 表目錄 VII 1. 緒言 1 1.1. 康唑類殺真菌劑 1 1.1.1. 三泰芬 3 1.1.2. 邁克尼 5 1.2. 三泰芬及邁克尼於環境中的宿命 7 1.3. 三泰芬及邁克尼之相關毒理研究 8 1.4. 細胞色素P450 10 1.5. 全反式視黃酸 13 1.6. 模式生物的選擇 15 1.7. 研究目的 15 2. 材料與方法 16 2.1. 試驗設計 16 2.2. 實驗動物 17 2.3. 藥品與試劑 17 2.4. 儀器 19 2.5. 暴露實驗設計與樣品採集 19 2.5.1. mRNA表現量分析預實驗 19 2.5.2. mRNA表現量分析實驗 20 2.5.3. CYP酵素活性分析實驗 20 2.6. 分析方法 21 2.6.1. mRNA表現量分析 21 2.6.1.1. RNA萃取 21 2.6.1.2. TURBO去DNA處理 21 2.6.1.3. 反轉錄 21 2.6.1.4. 引子設計與品質檢測 22 2.6.1.5. 即時定量聚合酶連鎖反應 23 2.6.2. CYP酵素活性分析 26 2.6.2.1. 微粒體萃取 26 2.6.2.2. EROD法 26 2.6.2.3. DBF試驗 26 2.7. 統計分析 27 3. 結果與討論 28 3.1. 引子品質及酵素分析條件測試 28 3.1.1. 引子品質測試 28 3.1.2. 酵素分析條件測試 28 3.2. mRNA表現量分析預實驗 35 3.3. 肝體指數 37 3.4. CYP1A基因表現及酵素活性 39 3.5. CYP3A基因表現及酵素活性 43 3.6. atRA恆定相關酵素之mRNA表現 48 3.7. atRA相關核受體之mRNA表現 50 3.8. p53之mRNA表現 53 4. 結論 55 參考文獻 56 | |
| dc.language.iso | zh-TW | |
| dc.title | 三泰芬及邁克尼干擾青鱂魚肝中細胞色素P450活性及全反式視黃酸相關基因表現量之研究 | zh_TW |
| dc.title | Triadimefon and Myclubutanil Interfere the Activity of Cytochrome P450s and mRNA Expression of All-trans Retinoic Acid-Related Genes in the Liver of Medaka (Oryzias latipes) | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 顏瑞泓,陳建德,廖秀娟,周佩欣 | |
| dc.subject.keyword | 三泰芬,邁克尼,細胞色素P450,全反式視黃酸,青鱂,魚, | zh_TW |
| dc.subject.keyword | Triadimefon,Myclobutanil,Cytochrome P450,All-trans-retinoic acid,medaka (Oryzias latipes), | en |
| dc.relation.page | 66 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2011-08-17 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 農業化學研究所 | zh_TW |
| 顯示於系所單位： | 農業化學系 | |
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| ntu-100-1.pdf | 2.52 MB | Adobe PDF | 檢視/開啟 |
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