請用此 Handle URI 來引用此文件：
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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 蔡志偉 | zh_TW |
| dc.contributor.advisor | Chi-Wei Tsai | en |
| dc.contributor.author | 羅薩菈 | zh_TW |
| dc.contributor.author | Sara Rebeca Puzul Trejo | en |
| dc.date.accessioned | 2023-08-08T16:37:13Z | - |
| dc.date.available | 2023-11-09 | - |
| dc.date.copyright | 2023-08-08 | - |
| dc.date.issued | 2023 | - |
| dc.date.submitted | 2023-07-13 | - |
| dc.identifier.citation | Ando H. 1910. On rice dwarf disease. J Japan Agri Soc 347: 1-3.
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Mol Plant Pathol 12: 938–954. Takami N. 1901. On dwarf disease of rice plant and “tsumaguro-yokabai”. J Jpn Agric Soc 241: 22-30. Weng SH, Tsai WS, Kenyon L, Tsai CW. 2015. Different transmission efficiencies may drive displacement of tomato begomovirus in the fields in Taiwan. Ann Appl Biol 166: 321-330. Wei J, Zhao JJ, Zhang T, Li FF, Ghanim M, Zhou XP, Ye GY, Liu SS, Wang XW. 2014. Specific cells in the primary salivary glands of the whitefly Bemisia tabaci control retention and transmission of begomoviruses. J Virol 88: 13460–13468. Yule S, Chiemsombat P, Srinivasan R. 2019. Detection of Tomato yellow leaf curl Thailand virus transmitted by Bemisia tabaci Asia I in tomato and pepper. Phytoparasitica 47: 143-153. | - |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/88171 | - |
| dc.description.abstract | none | zh_TW |
| dc.description.abstract | Bemisia tabaci, one of the most important whiteflies in terms of virus transmission, is commonly found in tropical and sub-tropical regions. The direct harm caused by. B. tabaci is linked to their feeding on the phloem sap of plants, resulting in a decrease in plant vigor. It also transmits over 300 species of plant viruses, including members of the genus Begomovirus. Begomoviruses pose significant risks to the cultivation of numerous vegetable, root, and fiber crops across tropical, subtropical, and temperate regions worldwide, emerging as serious threats to their production. Begomoviruses are transmitted exclusively by whiteflies. The mode of vector transmission of begomoviruses is generally believed to be persistent-circulative. For successful inoculation to healthy plants upon virus acquisition, begomoviruses must overcome the midgut and primary salivary gland barriers in B. tabaci. The objectives of this study were to examine: 1) the effect of acquisition time on virus infection in whitefly; 2) the effect of inoculation time on virus ejection by whitefly; and 3) the effect of the titer of source plant on virus infection in whitefly, ejection, and transmission by whitefly. The effect of acquisition time on the tomato yellow leaf curl Thailand virus (TYLCTHV) infection in the midgut and primary salivary glands (PSGs) of B. tabaci was evaluated by qPCR. The results showed the virus titer in the midgut increased from 1-h acquisition access period (AAP) to 24-h AAP, and the virus titer in the PSGs increased from 1-h AAP to 48-h AAP. The effect of inoculation time on the ejection of TYLCTHV with whitefly saliva was evaluated by the virus titer in the Chinese kale leaf disc that was fed by viruliferous whiteflies. The virus titer in the leaf disc was determined by qPCR. The virus titer in the leaf disc increased from 1-h AAP to 24-h AAP and then decreased to 48-h AAP. The effect of source plant titer on the TYLCTHV infection in the midgut and PSGs of B. tabaci was also evaluated by qPCR. The results showed the virus titers in the midguts of whiteflies that fed on source plants with the relative titers 14-16 and 9-11 were higher than that of whiteflies that fed on source plants with the relative titers 4-6. For PSG, the virus titers in the PSGs of whiteflies that fed on source plants with the relative titers 4-6 and 9-11 were higher than that of whiteflies that fed on source plants with the relative titers 14-16. The effect of source plant titer on the ejection of TYLCTHV with whitefly saliva was evaluated by the virus titer in the Chinese kale leaf disc that was fed by viruliferous whiteflies. The virus titer in the leaf disc was determined by qPCR. The results showed the virus titer in the leaf disc of the treatment source plant titers 14-16 was higher than that of the treatment source plant titers 4-6 and 9-11. The effect of source plant titer on the transmission rate of TYLCTHV by whitefly was evaluated by PCR. The results showed the transmission rates of three treatments were not significantly different among treatments. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2023-08-08T16:37:13Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2023-08-08T16:37:13Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | ABSTRACT i
CONTENTS iii LIST OF TABLE iv LIST OF FIGURES iv 1. INTRODUCTION 1 2. MATERIALS AND METHODS 4 2.1 Insects, plants, and virus 4 2.2 Crude extraction of DNA from tomato plants 5 2.3 Polymerase chain reaction 5 2.4 Effect of acquisition time on virus infection in whitefly 6 2.5 Effect of inoculation time on virus ejection by whitefly 6 2.6 Effect of source plant titer on virus infection in whitefly 7 2.7 Effect of source plant titer on virus ejection by whitefly 7 2.8 Effect of source plant titer on virus transmission by whitefly 8 2.9 DNA extraction from whiteflies 8 2.10 DNA extraction from plant tissues 9 2.11 Relative quantification of virus 9 2.12 Absolute quantification of virus 10 3. RESULTS 11 3.1 Effect of acquisition time on virus infection in whitefly 11 3.2 Effect of inoculation time on virus ejection by whitefly 11 3.3 Effect of source plant titer on virus infection in whitefly 12 3.4 Effect of source plant titer on virus ejection by whitefly 12 3.5 Effect of source plant titer on virus transmission by whitefly 13 4. DISCUSSION 13 5. REFERENCES 17 | - |
| dc.language.iso | en | - |
| dc.subject | 病毒接種 | zh_TW |
| dc.subject | 病媒傳染 | zh_TW |
| dc.subject | 豆類金黃嵌紋病毒屬 | zh_TW |
| dc.subject | 煙草粉蝨 | zh_TW |
| dc.subject | 病毒獲取 | zh_TW |
| dc.subject | vector transmission | en |
| dc.subject | Bemisia tabaci | en |
| dc.subject | virus acquisition | en |
| dc.subject | virus inoculation | en |
| dc.subject | Begomovirus | en |
| dc.title | 影響粉蝨傳播番茄黃捲葉泰國病毒的病毒獲取與接種因子 | zh_TW |
| dc.title | Factors affecting virus acquisition and inoculation of whitefly transmission of tomato yellow leaf curl Thailand virus | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 111-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 呂曉玲;林柏安 | zh_TW |
| dc.contributor.oralexamcommittee | Hsiao-Ling Lu;Po-An Lin | en |
| dc.subject.keyword | 豆類金黃嵌紋病毒屬,病媒傳染,煙草粉蝨,病毒獲取,病毒接種, | zh_TW |
| dc.subject.keyword | Begomovirus,vector transmission,Bemisia tabaci,virus acquisition,virus inoculation, | en |
| dc.relation.page | 30 | - |
| dc.identifier.doi | 10.6342/NTU202301490 | - |
| dc.rights.note | 同意授權(限校園內公開) | - |
| dc.date.accepted | 2023-07-14 | - |
| dc.contributor.author-college | 生物資源暨農學院 | - |
| dc.contributor.author-dept | 昆蟲學系 | - |
| 顯示於系所單位： | 昆蟲學系 | |
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