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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 蔡沛學 | zh_TW |
dc.contributor.advisor | Pei-shiue Tsai | en |
dc.contributor.author | 張祐佳 | zh_TW |
dc.contributor.author | Yu-Chia Chang | en |
dc.date.accessioned | 2023-07-19T16:13:58Z | - |
dc.date.available | 2023-11-09 | - |
dc.date.copyright | 2023-07-19 | - |
dc.date.issued | 2023 | - |
dc.date.submitted | 2023-05-03 | - |
dc.identifier.citation | 1. Galdikas, B. M. (1985). Subadult male orangutan sociality and reproductive behavior at Tanjung Puting. American Journal of Primatology, 8(2), 87-99.
2. Vandevoort, C. A., Neville, L. E., Tollner, T. L., & Field, L. P. (1993). Noninvasive semen collection from an adult orangutan. Zoo Biology, 12(3), 257-265. Rodrigues, 3. A.S.L., Pilgrim, J.D., Lamoreaux, J.L., Hoffmann, M. & Brooks, T.M. 2006. The value of the IUCN Red List for conservation. Trends in Ecology & Evolution 21(2): 71-76. 4. Wallace, P. Y., Asa, C. S., Agnew, M., & Cheyne, S. M. (2016). A review of population control methods in captive-housed primates. Animal Welfare, 25(1), 7-20. 5. Kusunoki, H., Daimaru, H., Minami, S., Nishimoto, S., Yamane, K. I., & Fukumoto, Y. (2001). Birth of a chimpanzee (Pan troglodytes) after artificial insemination with cryopreserved epididymal spermatozoa collected postmortem. Zoo biology, 20(3), 135-143. 6. Short, R. V. (1981). Sexual selection in man and the great apes. Reproductive biology of the great apes: Comparative and biomedical perspectives, 319-341. 7. Ghiglieri, M. P. (1987). Sociobiology of the great apes and the hominid ancestor. journal of Human Evolution, 16(4), 319-357. 8. Graham, C. (Ed.). (2012). Reproductive biology of the great apes: comparative and biomedical perspectives. Elsevier. 9. Gould, K. G., & Johnson‐Ward, J. (2000). Use of intrauterine devices (IUDs) for contraception in the common chimpanzee (Pan troglodytes). Journal of medical primatology, 29(2), 63-69. 10. Nadler, R. D. (1995). Proximate and ultimate influences on the regulation of mating in the great apes. American journal of primatology, 37(2), 93-102. 11. Maggioncalda, A. N., Czekala, N. M., & Sapolsky, R. M. (2000). Growth hormone and thyroid stimulating hormone concentrations in captive male orangutans: implications for understanding developmental arrest. American Journal of Primatology, 50(1), 67-76. 12. Morrell, J. M., & Hodges, J. K. (1998). Cryopreservation of non-human primate sperm: priorities for future research. Animal reproduction science, 53(1-4), 43-63. 13. Jang, T. H., Park, S. C., Yang, J. H., Kim, J. Y., Seok, J. H., Park, U. S., Choi, C. W., Lee, S. R., & Han, J. (2017). Cryopreservation and its clinical applications. Integrative medicine research, 6(1), 12-18. 14. Lima, J. S., Leão, D. L., Oliveira, K. G., Brito, A. B., Sampaio, W. V., Santos, R. R., ... & Domingues, S. F. (2017). Seminal coagulation and sperm quality in different social contexts in captive tufted capuchin monkeys (Sapajus apella). American journal of primatology, 79(6), e22643. 15. Prieto, M. T., Sanchez-Calabuig, M. J., Hildebrandt, T. B., Santiago-Moreno, J., & Saragusty, J. (2014). Sperm cryopreservation in wild animals. European journal of wildlife research, 60(6), 851-864. 16. Cocchia, N., Ciani, F., El-Rass, R., Russo, M., Borzacchiello, G., Esposito, V., ... & Lorizio, R. (2010). Cryopreservation of feline epididymal spermatozoa from dead and alive animals and its use in assisted reproduction. Zygote, 18(1), 1. 17. Silva, A. R., Lima, G. L., Peixoto, G. C. X., & Souza, A. L. P. (2015). Cryopreservation in mammalian conservation biology: current applications and potential utility. Research and Reports in Biodiversity Studies, 4, 1-8. 18. Vandevoort, C. A., Neville, L. E., Tollner, T. L., & Field, L. P. (1993). Noninvasive semen collection from an adult orangutan. Zoo Biology, 12(3), 257-265. 19. Bravo, P. W., Ccallo, M., & Garnica, J. (2000). The effect of enzymes on semen viscosity in llamas and alpacas. Small Ruminant Research, 38(1), 91-95. 20. Dixson, A. F., & Anderson, M. J. (2002). Sexual selection, seminal coagulation and copulatory plug formation in primates. Folia Primatologica, 73(2-3), 63-69. 21. Martan, J., & Shepherd, B. A. (1976). The role of the copulatory plug in reproduction of the guinea pig. Journal of Experimental Zoology, 196(1), 79-83. 22. Hodgkin, J., & Doniach, T. (1997). Natural variation and copulatory plug formation in Caenorhabditis elegans. Genetics, 146(1), 149-164. 23. Santiani, A., Huanca, W., Sapana, R., Huanca, T., Sepúlveda, N., & Sánchez, R. (2005). Effects on the quality of frozen‐thawed alpaca (Lama pacos) semen using two different cryoprotectants and extenders. Asian journal of Andrology, 7(3), 303-309. 24. Benirschke, K. (1984). The frozen zoo concept. Zoo Biology, 3(4), 325-328. 25. Ballou, J. D. (1992). Potential contribution of cryopreserved germ plasm to the preservation of genetic diversity and conservation of endangered species in captivity. Cryobiology, 29(1), 19-25. 26. Di Santo, M., Tarozzi, N., Nadalini, M., & Borini, A. (2011). Human sperm cryopreservation: update on techniques, effect on DNA integrity, and implications for ART. Advances in urology, 2012. 27. Gosálvez, J., Johnston, S., López-Fernández, C., Gosálbez, A., Arroyo, F., Fernández, J. L., & GÁlvarez, J. (2014). Sperm fractions obtained following density gradient centrifugation in human ejaculates show differences in sperm DNA longevity. Asian Pacific Journal of Reproduction, 3(2), 116-120. 28. Katkov, I. I., Isachenko, V., Isachenko, E., Kim, M. S., Lulat, A. G. I., Mackay, A. M., & Levine, F. (2006). Low-and high-temperature vitrification as a new approach to biostabilization of reproductive and progenitor cells. International Journal of Refrigeration, 29(3), 346-357. 29. Dupesh, S., Rasappan, P., Shila, S., & Gunasekaran, K. (2018). Human Sperm Freezing: Mini Update. Advances in Reproductive Sciences, 6(03), 59. 30. Morris, G. J. (2006). Rapidly cooled human sperm: no evidence of intracellular ice formation. Human Reproduction, 21(8), 2075-2083. 31. Dong, Q., Hill, D., & VandeVoort, C. A. (2009). Interactions among pre-cooling, cryoprotectant, cooling, and thawing for sperm cryopreservation in rhesus monkeys. Cryobiology, 59(3), 268-274. 32. Dong, Q., Correa, L. M., & VandeVoort, C. A. (2009). Rhesus monkey sperm cryopreservation with TEST-yolk extender in the absence of permeable cryoprotectant. Cryobiology, 58(1), 20-27. 33. Oldenhof, H., Friedel, K., Akhoondi, M., Gojowsky, M., Wolkers, W. F., & Sieme, H. (2012). Membrane phase behavior during cooling of stallion sperm and its correlation with freezability. Molecular membrane biology, 29(3-4), 95-106. 34. Gómez-Fernández, J., Gómez-Izquierdo, E., Tomás, C., Mocé, E., & de Mercado, E. (2012). Effect of different monosaccharides and disaccharides on boar sperm quality after cryopreservation. Animal reproduction science, 133(1-2), 109-116. 35. Golshahi, K., Aramli, M. S., Nazari, R. M., & Habibi, E. (2018). Disaccharide supplementation of extenders is an effective means of improving the cryopreservation of semen in sturgeon. Aquaculture, 486, 261-265. 36. Garde, J. J., Del Olmo, A., Soler, A. J., Espeso, G., Gomendio, M., & Roldan, E. R. S. (2008). Effect of egg yolk, cryoprotectant, and various sugars on semen cryopreservation in endangered Cuvier's gazelle (Gazella cuvieri). Animal reproduction science, 108(3-4), 384-401. 37. Isachenko, E., Isachenko, V., Katkov, I. I., Rahimi, G., Schondorf, T., Mallmann, P., ... & Nawroth, F. (2004). DNA integrity and motility of human spermatozoa after standard slow freezing versus cryoprotectant‐free vitrification. Human Reproduction, 19(4), 932-939. 38. Riva, N. S., Ruhlmann, C., Iaizzo, R. S., López, C. A. M., & Martínez, A. G. (2018). Comparative analysis between slow freezing and ultra-rapid freezing for human sperm cryopreservation. JBRA assisted reproduction, 22(4), 331. 39. Karthikeyan, M., Arakkal, D., Mangalaraj, A. M., & Kamath, M. S. (2019). Comparison of conventional slow freeze versus permeable cryoprotectant-free vitrification of abnormal semen sample: a randomized controlled trial. Journal of human reproductive sciences, 12(2), 150. 40. Mohamed, M. S. A. (2015). Slow cryopreservation is not superior to vitrification in human spermatozoa; an experimental controlled study. Iranian journal of reproductive medicine, 13(10), 633. 41. Li, Y. X., Zhou, L., Lv, M. Q., Ge, P., Liu, Y. C., & Zhou, D. X. (2019). Vitrification and conventional freezing methods in sperm cryopreservation: A systematic review and meta-analysis. European Journal of Obstetrics & Gynecology and Reproductive Biology, 233, 84-92. 42. Isachenko, V., Maettner, R., Petrunkina, A. M., Sterzik, K., Mallmann, P., Rahimi, G., ... & Isachenko, E. (2012). Vitrification of human ICSI/IVF spermatozoa without cryoprotectants: new capillary technology. Journal of andrology, 33(3), 462-468. 43. Kuznyetsov, V., Moskovtsev, S. I., Crowe, M., Lulat, A. G. M., & Librach, C. L. (2015). Vitrification of a small number of spermatozoa in normozoospermic and severely oligozoospermic samples. Systems biology in reproductive medicine, 61(1), 13-17. 44. Rall, W. F. (1993). Cryobiology of gametes and embryos from nonhuman primates. In In Vitro Fertilization and Embryo Transfer in Primates (pp. 223-245). Springer, New York, NY. 45. Isachenko, E., Isachenko, V., Katkov, I. I., Dessole, S., & Nawroth, F. (2003). Vitrification of mammalian spermatozoa in the absence of cryoprotectants: from past practical difficulties to present success. Reproductive biomedicine online, 6(2), 191-200. 46. Dong, Q., Correa, L. M., & VandeVoort, C. A. (2009). Rhesus monkey sperm cryopreservation with TEST-yolk extender in the absence of permeable cryoprotectant. Cryobiology, 58(1), 20-27. 47. Barbas, J. P., & Mascarenhas, R. D. (2009). Cryopreservation of domestic animal sperm cells. Cell and tissue banking, 10(1), 49-62. 48. Chaudhari, D. V., Dhami, A. J., Hadiya, K. K., & Patel, J. A. (2015). Relative efficacy of egg yolk and soya milk-based extenders for cryopreservation (− 196 C) of buffalo semen. Veterinary world, 8(2), 239. 49. Royere, D., Barthelemy, C., Hamamah, S., & Lansac, J. (1996). Cryopreservation of spermatozoa: a 1996 review. Human Reproduction Update, 2(6), 553-559. 50. Yu, J. F., Lai, Y. H., Wang, T. E., Wei, Y. S., Chang, Y. J., Li, S. H., ... & Tsai, P. S. (2018). The effects of type I collagenase on the degelification of chimpanzee (Pan troglodytes) semen plug and sperm quality. BMC veterinary research, 14(1), 1-12. 51. Çok, T., Aytaç, P. Ç., Şimşek, E., Haydardedeoğlu, B., Kalaycı, H., Özdemir, H., & Kılıçdağ, E. B. (2015). The effect of preserving prepared sperm samples at room temperature or at 37 C before intrauterine insemination (IUI) on clinical pregnancy rate. Turkish journal of obstetrics and gynecology, 12(1), 6. 52. Sukcharoen, N., Ngeamjirawat, J., Chanprasit, Y. E. N. C. H. I. T., & Aribarg, A. (1994). A comparison of Makler counting chamber and improved Neubauer hemocytometer in sperm concentration measurement. J Med Assoc Thai, 77(9), 471-476. 53. Kruger, T. F., Ackerman, S. B., Simmons, K. F., Swanson, R. J., Brugo, S. S., & Acosta, A. A. (1987). A quick, reliable staining technique for human sperm morphology. Archives of andrology, 18(3), 275-277. 54. Henkel, R., Schreiber, G., Sturmhoefel, A., Hipler, U. C., Zermann, D. H., & Menkveld, R. (2008). Comparison of three staining methods for the morphological evaluation of human spermatozoa. Fertility and sterility, 89(2), 449-455. 55. Pope, C. E., Zhang, Y. Z., & Dresser, B. L. (1991). A simple staining method for evaluating acrosomal status of cat spermatozoa. Journal of Zoo and Wildlife Medicine, 87-95. 56. Storey, B. T., Noiles, E. E., & Thompson, K. A. (1998). Comparison of glycerol, other polyols, trehalose, and raffinose to provide a defined cryoprotectant medium for mouse sperm cryopreservation. Cryobiology, 37(1), 46-58. 57. Anand, M., Baghel, G., & Yadav, S. (2017). Effect of egg yolk concentration and washing on sperm quality following cryopreservation in Barbari buck semen. Journal of Applied Animal Research, 45(1), 560-565. 58. Andrabi SMH. (2007) Fundamental principles of cryopreservation of Bos taurus and Bos indicus bull spermatozoa. Int J Agric and Biol 9, 367–369. 59. Pope, C. E., Zhang, Y. Z., & Dresser, B. L. (1991). A simple staining method for evaluating acrosomal status of cat spermatozoa. Journal of Zoo and Wildlife Medicine, 87-95. 60. Broekhuijse, M. L. W. J. (2012). Prediction of porcine male fertility. Utrecht University. 61. Laflamme, M. A., Gold, J., Xu, C., Hassanipour, M., Rosler, E., Police, S., ... & Murry, C. E. (2005). Formation of human myocardium in the rat heart from human embryonic stem cells. The American journal of pathology, 167(3), 663-671. 62. Cheng, F. P., Fazeli, A., Voorhout, W. F., arks, A., Bevers, M. M., & Colenbrander, B. E. N. (1996). Use of peanut agglutinin to assess the acrosomal status and the zona pellucida‐induced acrosome reaction in stallion spermatozoa. Journal of Andrology, 17(6), 674-682. 63. Al-Howiriny, T. A., Al-Sohaibani, M. O., El-Tahir, K. H., & Rafatullah, S. (2003). Preliminary evaluation of the anti-inflammatory and anti-hepatotoxic activities of'parsley'petroselinum crispum in rats. Journal of natural remedies, 3(1), 54-62. 64. Fen, C. T. C., Lee, S. N., Lim, M. N., & Yu, S. L. (2013). Relationship between Sperm Hyaluronan-binding Assay (HBA) Scores on Embryo Development, Fertilisation, and Pregnancy Rate in Patients Undergoing Intra-cytoplasmic Sperm Injection (ICSI). Proceedings of Singapore Healthcare, 22(2), 120-124. 65. World Health Organization. (2010). WHO laboratory manual for the examination and processing of human semen. 66. Broekhuijse, M. L. W. J., Šoštarić, E., Feitsma, H., & Gadella, B. M. (2012). Application of computer-assisted semen analysis to explain variations in pig fertility. Journal of animal science, 90(3), 779-789. 67. Schexnider, J. M., Baker, D. G., & Hasselschwert, D. L. (2007). Semen evaluation for verification of azoospermia after vasectomy in chimpanzees (Pan troglodytes). Journal of the American Association for Laboratory Animal Science, 46(5), 46-49. 68. Brewis, I. A., Boerke, A., Tsai, P. S., & Gadella, B. M. (2004). Sperm head membrane reorganisation during capacitation. International Journal of Developmental Biology, 52(5-6), 473-480. 69. Okabe, M. (2013). The cell biology of mammalian fertilization. Development, 140(22), 4471-4479. 70. Mortimer, D., Curtis, E. F., & Miller, R. G. (1987). Specific labelling by peanut agglutinin of the outer acrosomal membrane of the human spermatozoon. Reproduction, 81(1), 127-135. 71. Nakata, H., Wakayama, T., Asano, T., Nishiuchi, T., & Iseki, S. (2017). Identification of sperm equatorial segment protein 1 in the acrosome as the primary binding target of peanut agglutinin (PNA) in the mouse testis. Histochemistry and cell biology, 147(1), 27-38. 72. Dong, Q., Rodenburg, S. E., Huang, C., & Vandevoort, C. A. (2008). Cryopreservation of rhesus monkey (Macaca mulatta) epididymal spermatozoa before and after refrigerated storage. Journal of andrology, 29(3), 283-292. 73. Wildt, D. E., & Bush, M. (1984). Reproductive physiology studies in zoological species: concerns and strategies. Zoo biology, 3(4), 363-372. 74. Adams, G. P., Ratto, M. H., Collins, C. W., & Bergfelt, D. R. (2009). Artificial insemination in South American camelids and wild equids. Theriogenology, 71(1), 166-175. 75. Keel, B. A., & Webster, B. W. (1989). Semen analysis data from fresh and cryopreserved donor ejaculates: comparison of cryoprotectants and pregnancy rates. Fertility and sterility, 52(1), 100-105. 76. Subak, L. L., Adamson, G. D., & Boltz, N. L. (1992). Therapeutic donor insemination: a prospective randomized trial of fresh versus frozen sperm. American journal of obstetrics and gynecology, 166(6), 1597-1606. 77. Piomboni, P., Focarelli, R., Stendardi, A., Ferramosca, A., & Zara, V. (2012). The role of mitochondria in energy production for human sperm motility. International journal of andrology, 35(2), 109-124. Pilch, B., & Mann, M. (2006). Large-scale and high-confidence proteomic analysis of human seminal plasma. Genome biology, 7(5), 1-10. 78. Yokoo, M., & Sato, E. (2011). Physiological function of hyaluronan in mammalian oocyte maturation. Reproductive medicine and biology, 10(4), 221-229. 79. Opiela, J., Romanek, J., Lipiński, D., & Smorąg, Z. (2014). Effect of hyaluronan on developmental competence and quality of oocytes and obtained blastocysts from in vitro maturation of bovine oocytes. BioMed research international, 2014. 80. Erberelli, R. F., Salgado, R. M., Pereira, D. H. M., & Wolff, P. (2017). Hyaluronan-binding system for sperm selection enhances pregnancy rates in ICSI cycles associated with male factor infertility. JBRA assisted reproduction, 21(1), 2. 81. Best, B. P. (2015). Cryoprotectant toxicity: facts, issues, and questions. Rejuvenation research, 18(5), 422-436. 82. Bhattacharya, S. (2018). Cryoprotectants and their usage in cryopreservation process. Cryopreserv. Biotechnol. Biomed Biol. Sci, 7. 83. Aboagla, E. M. E., & Terada, T. (2004). Effects of egg yolk during the freezing step of cryopreservation on the viability of goat spermatozoa. Theriogenology, 62(6), 1160-1172. 84. Yan, Y., Ao, L., Wang, H., Duan, Y., Chang, S., Chen, B., ... & Si, W. (2016). Cryopreservation of Cynomolgus Macaque (Macaca fascicularis) Sperm by Using a Commercial Egg-Yolk–Free Freezing Medium. Journal of the American Association for Laboratory Animal Science, 55(6), 744-748. 85. Fabozzi, G., Starita, M. F., Rega, E., Alteri, A., Colicchia, A., Piscitelli, C., & Giannini, P. (2016). Evaluation of the efficiency of two different freezing media and two different protocols to preserve human spermatozoa from cryoinjury. International journal of reproductive medicine, 2016. 86. Anzar, M., Rajapaksha, K., & Boswall, L. (2019). Egg yolk-free cryopreservation of bull semen. PloS one, 14(10), e0223977. 87. Ptáček, M., Stádníková, M., Savvulidi, F., & Stádník, L. (2019). Ram Semen Cryopreservation Using Egg Yolk or Egg Yolk-free Extenders: Preliminary Results. Scientia agriculturae bohemica, 50(2), 96-103. 88. Gualtieri, R., Kalthur, G., Barbato, V., Di Nardo, M., Adiga, S. K., & Talevi, R. (2021). Mitochondrial Dysfunction and Oxidative Stress Caused by Cryopreservation in Reproductive Cells. Antioxidants, 10(3), 337. 89. Isachenko, E., Isachenko, V., Weiss, J. M., Kreienberg, R., Katkov, I. I., Schulz, M., ... & Sánchez, R. (2008). Acrosomal status and mitochondrial activity of human spermatozoa vitrified with sucrose. Reproduction, 136(2), 167-174. 90. Martinez, G., & Garcia, C. (2020). Sexual selection and sperm diversity in primates. Molecular and Cellular Endocrinology, 110974. 91. Warner, H., Martin, D. E., & Keeling, M. E. (1974). Electroejaculation of the great apes. Annals of biomedical engineering, 2(4), 419-432. | - |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/87742 | - |
dc.description.abstract | 黑猩猩與紅毛猩猩是世界上與人類親緣最接近的非人靈長類,然而,野外族群數量下降與原始棲地破壞等原因,都使這兩種物種都被國際自然保護聯盟瀕危物種紅色名錄列為瀕危物種。在域外保育部分,基於這兩種物種都有優勢個體才具較高交配權的原因,增加圈養族群數量成為域外保育中亟需解決之事。精液冷凍保存提供了一個高效率且能確保基因多樣性的解決方法。
然而,猩猩的精液在射精後即會形成凝膠,對精液冷凍保存造成了巨大影響。本篇論文旨在溶解精液形成的凝膠與隨之而來的精液冷凍保存方法之發展。根據我們實驗結果,在黑猩猩實驗中,使用0.1%第一型膠原蛋白酶,可以成功從凝膠化精液中更多溶解2.7倍之精子數,且並不影響活力、型態與受精能力相關參數。接下來,我們發展了一個使用卵黃當基礎冷凍液,並添加2.5%或7.8%甘油的慢速冷凍方式。結果顯示,在冷凍解凍後兩種方法皆可成功保存具良好品質之精液,2.5%甘油之方法,具有較高的頂體保護力;7.8%甘油之方法有較佳之活力與快速活動力。而之後,我們又改進了一個花費時間更短的快速冷凍方式且使用無添加卵黃的冷凍劑的冷凍方式。此方法與慢速冷凍法添加卵黃冷凍劑方法加相比,其具由相似的精子活力與保存更加的快速活動力、粒腺體功能性與受精能力相關參數。然而,在紅毛猩猩的實驗中,無論使用添加卵黃的慢速冷凍法搭配2.5%甘油、添加7.8%甘油,抑或是快速冷凍搭配無添加卵黃的方法,在冷凍解凍後皆不能成功保存良好的精子活力。 綜上所述,我們的研究顯示了第一型膠原蛋白酶可成功溶解黑猩猩凝膠化精液。慢速冷凍方法搭配添加卵黃冷凍劑,可成功保存黑猩猩精液。快速冷凍方法搭配無卵黃冷凍劑之方法可用於取代慢速冷凍方法搭配添加卵黃冷凍劑,此方法更容易操作,且保存粒線體保護能力更佳。 | zh_TW |
dc.description.abstract | Chimpanzees (Pan troglodytes) and Orangutans (Pongo pygmaeus), have the most similar gene profile in non-human primates (NHP) to human. However, both species are endangered species on IUCN red list due to decreasing total populations and increasing destroy of their natural habitats. In both species, due to the dominant hierarchy male has the priority and exclusive mating right, which greatly affects the number of individuals within the population and subsequently decreases genes diversity of captive groups live in the extraterritorial conservation, alternative solution other than natural mating is urgently needed. To overcome these obstacles, sperm cryopreservation provides an effective way to preserve valuable genetic resources and to develop subsequent assisted reproductive technologies.
However, semen ejaculates forming solid gel-like plug after ejaculation causes the difficulty for sperm cryopreservation. This thesis focuses on liquefaction of colloid semen plug and subsequently improve cryopreservation protocols for both chimpanzees and orangutans. According to our result, we showed 0.1% type I collagenase can successfully degelificated semen plug and released 2.7-fold more sperm cells without affected sperm motility, morphology, and fertilization relative parameter in chimpanzees. Subsequently, we demonstrated that the long process protocol with egg yolk -based cryoprotectant added with 2.5% and 7.8% glycerol can be used in chimpanzee and are favorable for different aspects of sperm physiology. The 2.5% protects sperm acrosome integrity with superior result, and 7.8% results in better sperm motility, progressive motility. Moreover, we also showed that improved shorter protocol with egg yolk-free cryoprotectant displayed similar motility and better progressive motility, mitochondrial functionality and fertilization relative parameter when compared with the traditional long and time-consuming protocol. However, no matter egg yolk containing, or egg yolk-free medium used in long process or short-term protocol cannot be applied to freeze orangutan semen as low sperm motility was observed after the cryopreservation procedure. In conclusions, our study demonstrated the type I collagenase could be used in semen liquefication in chimpanzees. The egg yolk medium with long process protocol can be used to preserved chimpanzee semen. The egg yolk-free medium with improve protocol can also be used to replaced egg yolk medium with beneficial effects in terms of shorter sperm handling time and better preservation of sperm mitochondria. | en |
dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2023-07-19T16:13:58Z No. of bitstreams: 0 | en |
dc.description.provenance | Made available in DSpace on 2023-07-19T16:13:58Z (GMT). No. of bitstreams: 0 | en |
dc.description.tableofcontents | 論文口試委員審定書 i
致謝 ii Abstract iii 中文摘要 vi Contents vii List of figures ix Chapter 1 Introduction 1 1 Natural social structure and the importance of great apps conservation 1 2 Semen collection 3 3 Semen plug 7 3.1 Introduction of semen plug 7 3.2 The positive aspects and the biological meaning of semen plug 7 3.3 The negative aspects of semen plug 7 4 Sperm cryopreservation 8 4.1 The importance of cryopreservation 8 4.2 Cryopreservation strategies 9 4.3 Advantages and disadvantages for different cryopreservation strategies 11 Chapter 2 Material and methods 14 1 Animal (Chimpanzee and orangutan) training and semen collection 14 2 Chemicals, reagents, antibodies 16 3 Semen evaluations and sperm preparation 17 4 Sperm viability assay 21 5 Computer-assisted sperm analysis (CASA) 22 6 Indirect immunofluorescent (IFA) assays for sperm capacitation status and acrosome integrity 23 7 Mitochondria status assessment 24 8 Immunoblotting 26 9 Hyaluronan biding assay (HBA Assay) 26 10 Statistical analyses 28 Chapter 3 Result 29 1 Type I collagenase facilitated the release of spermatozoa from colloid semen without affecting sperm morphology and viability 29 2 Motility-related sperm parameters were not altered by type I collagenase treatment 31 3 Type I collagenase did not induce spontaneous sperm capacitation or acrosome reaction 34 4 Type I collagenase did not alter mitochondrial functionality and sperm binding ability 37 5 Collagenase-treated spermatozoa showed similar morphological and physical characteristics as un-treated spermatozoa after glycerol-based cryopreservation 40 6 Glycerol concentrations used as cryoprotectant benefit for different aspects of chimpanzee spermatozoa 43 7 Glycerol concentrations used as cryoprotectant benefit for different aspects of chimpanzee spermatozoa 46 8 Motility-related sperm parameters were not altered in egg yolk-free medium 49 9 Sperm characteristics were not altered in egg yolk-free medium 51 10 Egg yolk free medium did not alter sperm mitochondrial functionality and oocyte binding related characteristic. 52 Chapter 4 Discussion 54 Chapter 5 Future work 64 Reference 67 List of figures Figure 1. Evolutionary taxonomy of non-human primates. (James Venner., 2018) 1 Figure 2. Semen collection using hand-massage approach 6 Figure 3. Colloid semen plug (white gelatinous structure) collected from chimpanzee using artificial vagina 6 Figure 4. Spermatozoa of different animal species exhibit various shape and size which will lead to variable cryopreservation protocols. 11 Figure 5 Semen collection from Pan troglodytes using artificial vagina. 15 Figure 6 The experimental flow of semen evaluation and preparation 20 Figure 7 Type I collagenase facilitated the release of spermatozoa from colloid semen. 30 Figure 8 Type I collagenase facilitated the release of spermatozoa from colloid semen without affecting sperm morphology and viability. 31 Figure 9 CASA analyses revealed no negative effects of type I collagenase on sperm motility-related parameters. 33 Figure 10 Type I collagenase treatment did not result in spontaneous capacitation or acrosome reaction. 36 Figure 11 Type I collagenase did not alter mitochondrial functionality and sperm binding ability. 39 Figure 12 Analyses on cryopreserved chimpanzee spermatozoa revealed no differences between control and collagenase-treated groups. 42 Figure 13 Collagenase-treated sperm cells exhibited relatively stable motility, progressive motility, beat frequency cross and linearity within 1 h post thaw. 45 Figure 14 Glycerol concentrations resulted in different protective effects on either sperm membrane integrity or motility-related characteristics. 48 Figure 15 Sperm cells in egg yolk free medium (eyF) exhibited relatively stable motility, beat frequency cross and linearity after post-thawed procedure. 50 Figure 16 Egg yolk free medium did not alter sperm characteristics. 52 Figure 17 Egg yolk free medium did not alter mitochondrial functionality and sperm binding ability. 53 Figure 18 Current cryopreservation protocols were not suitable for Orangutan. 66 | - |
dc.language.iso | en | - |
dc.title | 優化黑猩猩精液冷凍保存方法 | zh_TW |
dc.title | Improvement of Cryopreservation Protocol on Chimpanzee Sperm | en |
dc.type | Thesis | - |
dc.date.schoolyear | 111-2 | - |
dc.description.degree | 碩士 | - |
dc.contributor.oralexamcommittee | 李勝祥;張惠雯;余珍芳 | zh_TW |
dc.contributor.oralexamcommittee | Sheng-Hsiang Li;Hui-wen Chang;Jane-Fang Yu | en |
dc.subject.keyword | 黑猩猩,紅毛猩猩,精子,膠原蛋白酶,精液冷凍保存,無卵黃, | zh_TW |
dc.subject.keyword | chimpanzees,orangutans,sperm,collagenase,cryopreservation,egg yolk-free, | en |
dc.relation.page | 82 | - |
dc.identifier.doi | 10.6342/NTU202300735 | - |
dc.rights.note | 同意授權(限校園內公開) | - |
dc.date.accepted | 2023-05-04 | - |
dc.contributor.author-college | 生物資源暨農學院 | - |
dc.contributor.author-dept | 獸醫學系 | - |
顯示於系所單位: | 獸醫學系 |
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