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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/87737完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 楊東霖 | zh_TW |
| dc.contributor.advisor | Tony Yang | en |
| dc.contributor.author | 王建邦 | zh_TW |
| dc.contributor.author | Chien-Pang Wang | en |
| dc.date.accessioned | 2023-07-19T16:12:18Z | - |
| dc.date.available | 2023-11-09 | - |
| dc.date.copyright | 2023-07-19 | - |
| dc.date.issued | 2023 | - |
| dc.date.submitted | 2023-05-30 | - |
| dc.identifier.citation | Van De Weghe, J.C., A. Gomez, and D. Doherty, The Joubert-Meckel-Nephronophthisis Spectrum of Ciliopathies. Annu Rev Genomics Hum Genet, 2022. 23: p. 301-329.
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Dean, S., et al., Cilium transition zone proteome reveals compartmentalization and differential dynamics of ciliopathy complexes. Proc Natl Acad Sci U S A, 2016. 113(35): p. E5135-43. Pratt, M.B., et al., Drosophila sensory cilia lacking MKS proteins exhibit striking defects in development but only subtle defects in adults. J Cell Sci, 2016. 129(20): p. 3732-3743. Wang, W.J., et al., CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base. Nat Cell Biol, 2013. 15(6): p. 591-601. Fürstenberg, A. and M. Heilemann, Single-molecule localization microscopy-near-molecular spatial resolution in light microscopy with photoswitchable fluorophores. Phys Chem Chem Phys, 2013. 15(36): p. 14919-30. Gogendeau, D., et al., MKS-NPHP module proteins control ciliary shedding at the transition zone. PLoS Biol, 2020. 18(3): p. e3000640. 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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/87737 | - |
| dc.description.abstract | 過渡區（TZ）位於初級纖毛基部（basal body）的特化區域，為控制和纖毛生成相關蛋白進出胞器的擴散屏障。到目前為止，在TZ中招募的許多蛋白質在空間排列上仍然不明確。單分子定位顯微技術（SMLM）之發展使生物學家能夠在奈米尺度下研究大分子結構。通過有效減少點擴散函數間的重疊使得定位精確度提升，單分子定位顯微技術突破光學顯微鏡的繞射極限並實現十至二十奈米的解析度。近年問世的擴展顯微技術使得超解析顯微的解析度獲得更進一步的提升。
本研究使用結合dSTORM（direct stochastic optical reconstruction microscopy）及U-ExM（ultra-expansion microscopy）的Ex-dSTORM同時在光學上及樣本上提升解析度，來建立哺乳類過渡區五種蛋白在軸向及徑向上的分布。我們發現CEP290在Ex-STORM的解析度下顯示了兩層在以前它被認為是厚的單層結構，而這可能與纖毛的生長狀態高度相關。基底層皆存在於纖毛生長之前的母中心粒和子中心粒上，遠端層伴隨著纖毛生長出現和其他TZ蛋白募集。在這裡，我們使用位於DAP的CEP164作為空間上的參考基準，描述了五個TZ蛋白的縱向和橫向分佈的覆蓋範圍。 | zh_TW |
| dc.description.abstract | As a specialized region located at the root of the cilium, transition zone serves as a diffusion barrier that regulates proteins associated with ciliopathy entry and exit from the organelle. So far, there are numerous proteins recruited in TZ remain ambiguous in the spatial arrangement. The development of single-molecule localization microscopy (SMLM) has enabled biologists to investigate substructures at the nanoscale. By effectively reducing the overlap between point spread functions to improve positioning accuracy, single-molecule localization microscopy surpasses the diffraction limit of conventional optical microscopy, enabling a resolution of ten to twenty nanometers. The expansion microscopy that came out in recent years has further improved the resolution of super-resolution microscopy.
In this research, Ex-STORM, which is combined with dSTORM (direct stochastic optical reconstruction microscopy) and U-ExM (ultra-expansion microscopy), was utilized to improve the resolution optically and on the sample simultaneously. We found that CEP290 shows two layers under the resolution of Ex-STORM while it is thought to be one thick layer before. It could be highly related to the growing state of cilium. The basal layer exists on both mother and daughter centrioles before ciliogenesis, and the distal layer appears with the cilium growth and TZ proteins recruitment. Here, we depicted the coverage of five TZ proteins with CEP164, which belongs to the DAPs. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2023-07-19T16:12:18Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2023-07-19T16:12:18Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | CONTENTS
口試委員會審定書 i 致謝 ii 中文摘要 iii ABSTRACT iv CONTENTS vi LIST OF FIGURES vii LIST OF TABLES ix Chapter 1 Introduction 1 1.1 Cilium 1 1.2 Ciliogenesis 3 1.3 Ciliopathy 8 1.4 Transition Zone (TZ) 13 1.5 Diffraction limit and resolution 24 1.6 Single-Molecular Localization Microscopy (SMLM) 26 1.7 Expansion Microscopy (ExM) 29 Chapter 2 Materials and Methods 32 2.1 Reagent 32 2.2 Antibodies 32 2.3 Workflow 33 2.4 Cell culture 34 2.5 Sample preparation 34 2.6 Ultra-Expansion Microscopy (U-ExM) 35 Chapter 3 Results 41 3.1 Results of U-ExM 41 3.2 Results of Ex-dSTORM 46 3.3 Difficulties on imaging TZ proteins at top view 50 3.4 Probability distribution images based on the side views of TZ proteins 51 3.5 Quantification analysis 53 3.6 Axial analysis 55 3.7 Lateral analysis and the two-dimensional probability distribution model 57 Chapter 4 Discussion 61 REFERENCE 64 | - |
| dc.language.iso | en | - |
| dc.subject | 超解析技術 | zh_TW |
| dc.subject | 纖毛疾病 | zh_TW |
| dc.subject | 初級纖毛過渡區 | zh_TW |
| dc.subject | 擴展顯微技術 | zh_TW |
| dc.subject | 單分子定位 | zh_TW |
| dc.subject | direct stochastic reconstruction microscopy (dSTORM) | en |
| dc.subject | primary cilium | en |
| dc.subject | ciliary transition zone | en |
| dc.subject | ultra-expansion microscopy (U-ExM) | en |
| dc.subject | single-molecular localization microscopy (SMLM) | en |
| dc.subject | super-resolution | en |
| dc.subject | ciliopathy | en |
| dc.title | 單分子定位顯微鏡揭示哺乳動物過渡區蛋白在分子解析度下的空間排列 | zh_TW |
| dc.title | Single-molecule localization microscopy reveals the spatial arrangement of the mammalian transition zone proteins at molecular resolution | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 111-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 黃念祖;王婉菁 | zh_TW |
| dc.contributor.oralexamcommittee | Nien-Tsu Huang;Won-Jing Wang | en |
| dc.subject.keyword | 超解析技術,單分子定位,擴展顯微技術,初級纖毛過渡區,纖毛疾病, | zh_TW |
| dc.subject.keyword | super-resolution,single-molecular localization microscopy (SMLM),direct stochastic reconstruction microscopy (dSTORM),ultra-expansion microscopy (U-ExM),primary cilium,ciliary transition zone,ciliopathy, | en |
| dc.relation.page | 71 | - |
| dc.identifier.doi | 10.6342/NTU202300903 | - |
| dc.rights.note | 同意授權(限校園內公開) | - |
| dc.date.accepted | 2023-05-31 | - |
| dc.contributor.author-college | 電機資訊學院 | - |
| dc.contributor.author-dept | 生醫電子與資訊學研究所 | - |
| 顯示於系所單位： | 生醫電子與資訊學研究所 | |
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