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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/8659完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 譚婉玉(Woan-Yuh Tarn) | |
| dc.contributor.author | Kuo-Ming Lee | en |
| dc.contributor.author | 李國銘 | zh_TW |
| dc.date.accessioned | 2021-05-20T19:59:26Z | - |
| dc.date.available | 2013-09-09 | |
| dc.date.available | 2021-05-20T19:59:26Z | - |
| dc.date.copyright | 2010-09-09 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-06-18 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/8659 | - |
| dc.description.abstract | TRAP150起初被發現為轉錄活化複合體TRAP150/Mediator的一個次單位。此外，TRAP150亦被發現為剪接體的其中一員，說明其可能在訊息核醣核酸剪接中扮演特定的角色。我們最近的報導指出，TRAP150可與其他剪接分子共定位於細胞核內的核斑點中。TRAP150不僅是活體內訊息核醣核酸剪接過程所必須，而其大量表現時，更可大幅活化此剪接過程。我們發現訊息核醣核酸剪接完成之後，TRAP150仍與剪接產物結合，我們亦發現TRAP150可直接與數個exon jounction complex的組成蛋白及運送訊息核醣核酸出核的受器TAP進行結合。此觀察指出TRAP150可能具有調控訊息核醣核酸剪接後事件的功能。當TRAP150被束縛在先驅訊息核醣核酸上時，TRAP150可促進剪接完成的訊息核醣核酸的降解。然而，與典型的無意義媒介訊息核醣核酸降解機制不同之處為，TRAP150調控的訊息核醣核酸降解不依賴轉譯，且發生在細胞核中。儘管TRAP150可藉由不同的獨立區塊活化訊息核醣核酸的剪接以及訊息核醣核酸的降解，我們並不清楚此二功能確切的運作機制。最近，透過尋找TRAP150結合蛋白，我們發現數個腺苷酸化相關蛋白質與其結合。因此，TRAP150可能會協調細胞核內核醣核酸處理過程的不同步驟，並且使異常加工的核醣核酸降解。 | zh_TW |
| dc.description.abstract | TRAP150 was initially identified as a subunit of the transcription coactivator TRAP/Mediator complex. In addition, TRAP150 has also been detected as a component of the spliceosome, suggesting its role in pre-mRNA splicing. We recently reported that TRAP150 colocalizes with splicing factors in nuclear speckles. TRAP150 is not only required for pre-mRNA splicing in vivo, but also substantially activates splicing when overexpressed. We found that TRAP150 remains bound to the spliced mRNA after splicing and accordingly interacts directly with several components of the exon junction complex and the mRNA export receptor TAP. This observation suggests that TRAP150 has a post-splicing function. When tethered to a precursor mRNA, TRAP150 could induce degradation of the spliced mRNA. However, in contrast to the canonical nonsense-mediated decay, TRAP150-mediated mRNA decay is translation independent and occurs in the nucleus. TRAP150 activates pre-mRNA splicing and mRNA degradation via distinct domains, but how it exactly acts in these two events is not yet clear. We recently screened for TRAP150-interacting proteins and found that TRAP150 also associated with polyadenylation factors. Therefore, TRAP150 may function in coordinating different steps of nuclear mRNA processing and perhaps target aberrantly processed mRNAs for degradation. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-20T19:59:26Z (GMT). No. of bitstreams: 1 ntu-99-D94448004-1.pdf: 9097697 bytes, checksum: 117c3b8587b99340cd74d02a07288253 (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | 口試委員會審定書 i
中文摘要 ii Abstract iii 1. Introduction 1 1.1. Pol II CTD in transcription-coupled processes 1 1.2. Coupling TREX complex in mRNP synthesis 5 1.3. Versatile EJC in mRNP metabolism 7 1.4. Unusual but critical TAP for the export of mRNAs 9 2. Materials and 13 2.1. Plasmids 13 2.2. Antibody preparation 14 2.3. Cell culture and transfection 14 2.4. Indirect immunofluorescence and heterokaryon assay 15 2.5. In vivo splicing and RT-PCR 15 2.6. In vitro splicing/tethering assay and immunoprecipitation of the spliceosome 17 2.7. Immunoprecipitation 18 2.8. NMD assays and northern blotting 19 3. Results 22 3.1. Verification of TAP-TRAP150 interaction 22 3.1.1. TRAP150 is a TAP-interacting phosphorylated protein 22 3.1.2 TRAP150 is a nuclear-restricted protein with speckle localization 23 3.2. Characterization of TRAP150’s function in splicing 24 3.2.1. TRAP150 activates splicing in vivo 24 3.2.2. TRAP150 is required for splicing in vivo 25 3.2.3. TRAP150 could not regulate alternative splicing 26 3.3. In vitro analysis of the properties of TRAP150 in splicing regulation 27 3.3.1. TRAP150 is not required for in vitro splicing 27 3.3.2. TRAP150 joins the spliceosome at the earlier stage and associates with spliced RNA 28 3.3.3. TRAP150 associates with components of EJC 29 3.4. Investigation of RNA decay mediated by TRAP150 30 3.4.1. TRAP150 induces mRNA decay in tethering NMD assay 30 3.4.2. C-terminal conserved region of TRAP150 is required for mRNA degradation 32 3.4.3. TRAP150-mediated degradation occurs in a translation-independent manner 32 3.4.4. TRAP150 is not required for NMD and induces mRNA degradation in the nucleus 33 3.4.5. Neither 5’-to-3’ nor 3’-to-5’ exonucleolytic attack involves in TRAP150-mediated decay 34 3.4.6. The mRNA decay regulated by TRAP150 might be distinct from DRN 36 3.4.7. TRAP150-mediated degradation is not coupled to splicing 37 3.5. Identification of TRAP150 associated protein 38 3.5.1. TRAP150 interacts with several groups of mRNA processing factors 38 3.5.2. TRAP150 associates with CPSF73 40 3.6. Global analysis of gene-expression profiles under knockdown of TRAP150 40 3.6.1. TRAP150 affects the mRNA level of several mRNA processing factors 41 3.6.2. TRAP150 associates with the mRNPs of each candidate gene 42 3.6.3. An inverse relationship stands for the expression of TRAP150 and BCLAF1 43 4. Discussion 44 4.1. Role of TRAP150 in pre-mRNA splicing 44 4.2. Role of TRAP150 in mRNA decay 47 4.3. Roles of TRAP150 in other mRNA processing steps 49 Reference 53 Figures 64 Table 83 Supplemental Figures 88 Appendix 91 | |
| dc.language.iso | en | |
| dc.title | TRAP150在細胞核核醣核酸處理過程的功能探討 | zh_TW |
| dc.title | Characterization of the roles of TRAP150 in nuclear mRNA processing | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 博士 | |
| dc.contributor.oralexamcommittee | 李芳仁(Fang-Jen S. Lee),鄭淑珍(Soo-Chen Cheng),林淑端(Sue Lin-Chao),王桂馨(Guey-Shin Wang) | |
| dc.subject.keyword | 先驅訊息核醣核酸剪接,訊息核醣核酸降解,剪接體,exon-junction complex,訊息核醣核酸處理, | zh_TW |
| dc.subject.keyword | pre-mRNA splicing,mRNA decay,spliceosome,exon-junction complex,nuclear mRNA processing, | en |
| dc.relation.page | 102 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2010-06-18 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 分子醫學研究所 | zh_TW |
| 顯示於系所單位： | 分子醫學研究所 | |
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