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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 劉瑞芬(Ruey-Fen Liou) | |
dc.contributor.advisor | 劉瑞芬(Ruey-Fen Liou | rfliou@ntu.edu.tw | ), | |
dc.contributor.author | Jia-Ji Tan | en |
dc.contributor.author | 檀佳季 | zh_TW |
dc.date.accessioned | 2023-03-19T23:30:07Z | - |
dc.date.copyright | 2022-09-27 | |
dc.date.issued | 2022 | |
dc.date.submitted | 2022-09-21 | |
dc.identifier.citation | Adachi, H., Nakano, T., Miyagawa, N., Ishihama, N., Yoshioka, M., Katou, Y., Yaeno, T., Shirasu, K., and Yoshioka, H. 2015. WRKY transcription factors phosphorylated by MAPK regulate a plant immune NADPH oxidase in Nicotiana benthamiana. Plant Cell 27:2645-2663. Armstrong, M. R., Whisson, S. C., Pritchard, L., Bos, J. I., Venter, E., Avrova, A. O., Rehmany, A. P., Böhme, U., Brooks, K., and Cherevach, I. 2005. An ancestral oomycete locus contains late blight avirulence gene Avr3a, encoding a protein that is recognized in the host cytoplasm. Proc. Natl. Acad. Sci. U. S. A. 102:7766-7771. Bi, G., Liebrand, T. W., Bye, R. R., Postma, J., van der Burgh, A. M., Robatzek, S., Xu, X., and Joosten, M. H. 2016. SOBIR1 requires the GxxxG dimerization motif in its transmembrane domain to form constitutive complexes with receptor‐like proteins. Mol. Plant Pathol. 17:96-107. Bisceglia, N. G., Gravino, M., and Savatin, D. V. 2015. Luminol-based assay for detection of immunity elicitor-induced hydrogen peroxide production in Arabidopsis thaliana leaves. Bio-protocol 5:e1685-e1685. Block, A., Toruño, T. Y., Elowsky, C. G., Zhang, C., Steinbrenner, J., Beynon, J., and Alfano, J. R. 2014. The Pseudomonas syringae type III effector Hop D 1 suppresses effector‐triggered immunity, localizes to the endoplasmic reticulum, and targets the Arabidopsis transcription factor NTL 9. New Phytol. 201:1358-1370. Boissy, G., de La Fortelle, E., Kahn, R., Huet, J.-C., Bricogne, G., Pernollet, J.-C., and Brunie, S. 1996. Crystal structure of a fungal elicitor secreted by Phytophthora cryptogea, a member of a novel class of plant necrotic proteins. Structure. 4:1429-1439. Boller, T., and Felix, G. 2009. A renaissance of elicitors: perception of microbe-associated molecular patterns and danger signals by pattern-recognition receptors. Annu. Rev. Plant Biol. 60:379-406. Bonza, M. C., Morandini, P., Luoni, L., Geisler, M., Palmgren, M. G., and De Michelis, M. I. 2000. At-ACA8 encodes a plasma membrane-localized calcium-ATPase of Arabidopsis with a calmodulin-binding domain at the N terminus. Plant Physiol. 123:1495-1506. Bos, J. I., Armstrong, M. R., Gilroy, E. M., Boevink, P. C., Hein, I., Taylor, R. M., Zhendong, T., Engelhardt, S., Vetukuri, R. R., and Harrower, B. 2010. Phytophthora infestans effector AVR3a is essential for virulence and manipulates plant immunity by stabilizing host E3 ligase CMPG1. Proc. Natl. Acad. Sci. U. S. A. 107:9909-9914. Bos, J. I., Kanneganti, T. D., Young, C., Cakir, C., Huitema, E., Win, J., Armstrong, M. R., Birch, P. R., and Kamoun, S. 2006. The C‐terminal half of Phytophthora infestans RXLR effector AVR3a is sufficient to trigger R3a‐mediated hypersensitivity and suppress INF1‐induced cell death in Nicotiana benthamiana. Plant J. 48:165-176. Brandizzi, F., Hanton, S., DaSilva, L. L. P., Boevink, P., Evans, D., Oparka, K., Denecke, J., and Hawes, C. 2003. ER quality control can lead to retrograde transport from the ER lumen to the cytosol and the nucleoplasm in plants. Plant J. 34:269-281. Chang, Y. H., Yan, H. Z., and Liou, R. F. 2015. A novel elicitor protein from Phytophthora parasitica induces plant basal immunity and systemic acquired resistance. Mol. Plant Pathol. 16:123-136. Chaparro-Garcia, A., Schwizer, S., Sklenar, J., Yoshida, K., Petre, B., Bos, J. I., Schornack, S., Jones, A. M., Bozkurt, T. O., and Kamoun, S. 2015. Phytophthora infestans RXLR-WY effector AVR3a associates with dynamin-related protein 2 required for endocytosis of the plant pattern recognition receptor FLS2. PLoS One 10. Doi: https://doi.org/10.1371/journal.pone.0137071 Chaparro-Garcia, A., Wilkinson, R. C., Gimenez-Ibanez, S., Findlay, K., Coffey, M. D., Zipfel, C., Rathjen, J. P., Kamoun, S., and Schornack, S. 2011. The receptor-like kinase SERK3/BAK1 is required for basal resistance against the late blight pathogen Phytophthora infestans in Nicotiana benthamiana. PLoS One 6. Doi: https://doi.org/10.1371/journal.pone.0016608 Cook, D. E., Mesarich, C. H., and Thomma, B. P. 2015. Understanding plant immunity as a surveillance system to detect invasion. Annu. Rev. Phytopathol. 53:541-563. Couto, D., and Zipfel, C. 2016. Regulation of pattern recognition receptor signaling in plants. Nat. Rev. Immunol. 16:537. Derevnina, L., Dagdas, Y. F., De la Concepcion, J. C., Bialas, A., Kellner, R., Petre, B., Domazakis, E., Du, J., Wu, C. H., and Lin, X. 2016. Nine things to know about elicitins. New Phytol. 212:888-895. Dokládal, L., Obořil, M., Stejskal, K., Zdráhal, Z., Ptáčková, N., Chaloupková, R., Damborský, J., Kašparovský, T., Jeandroz, S., and Žd'árská, M. 2012. Physiological and proteomic approaches to evaluate the role of sterol binding in elicitin-induced resistance. J. Exp. Bot. 63:2203-2215. Domazakis, E., Wouters, D., Lochman, J., Visser, R. G., Joosten, M. H., and Vleeshouwers, V. G. 2020. ELR is a true pattern recognition receptor that associates with elicitins from diverse Phytophthora species. BioRxiv. Doi: https://doi.org/10.1101/2020.09.21.305813 Domazakis, E., Wouters, D., Visser, R. G., Kamoun, S., Joosten, M. H., and Vleeshouwers, V. G. 2018. The ELR-SOBIR1 complex functions as a two-component receptor-like kinase to mount defense against Phytophthora infestans. Mol. Plant Microbe Interact. 31:795-802. Du, J., Verzaux, E., Chaparro-Garcia, A., Bijsterbosch, G., Keizer, L. P., Zhou, J., Liebrand, T. W., Xie, C., Govers, F., and Robatzek, S. 2015. Elicitin recognition confers enhanced resistance to Phytophthora infestans in potato. Nat. Plants. 1:1-5. Dunyak, B. M., and Gestwicki, J. E. 2016. Peptidyl-Proline Isomerases (PPIases): Targets for natural products and natural product-inspired compounds: miniperspective. J. Med. Chem. 59:9622-9644. El-Kharbotly, A., Leonards-Schippers, C., Huigen, D., Jacobsen, E., Pereira, A., Stiekema, W., Salamini, F., and Gebhardt, C. 1994. Segregation analysis and RFLP mapping of the R1 and R3 alleles conferring race-specific resistance to Phytophthora infestans in progeny of dihaploid potato parents. Mol. Gen. Genet. 242:749-754. Fan, G., Yang, Y., Li, T., Lu, W., Du, Y., Qiang, X., Wen, Q., and Shan, W. 2018. A Phytophthora capsici RXLR effector targets and inhibits a plant PPIase to suppress endoplasmic reticulum-mediated immunity. Mol. Plant. 11:1067-1083. Fawke, S., Doumane, M., and Schornack, S. 2015. Oomycete interactions with plants: infection strategies and resistance principles. Microbiol. Mol. Biol. Rev. 79:263-280. Gao, M., Wang, X., Wang, D., Xu, F., Ding, X., Zhang, Z., Bi, D., Cheng, Y. T., Chen, S., and Li, X. 2009. Regulation of cell death and innate immunity by two receptor-like kinases in Arabidopsis. Cell Host Microbe. 6:34-44. Gust, A. A., and Felix, G. 2014. Receptor like proteins associate with SOBIR1-type of adaptors to form bimolecular receptor kinases. Curr. Opin. Plant Biol. 21:104-111. Gust, A. A., Pruitt, R., and Nürnberger, T. 2017. Sensing danger: key to activating plant immunity. Trends Plant Sci. 22:779-791. Hendrix, J. 1970. Sterols in growth and reproduction of fungi. Annu. Rev. Phytopathol. 8:111-130. Hong, B., Ichida, A., Wang, Y., Scott Gens, J., Pickard, B. G., and Harper, J. F. 1999. Identification of a calmodulin-regulated Ca2+-ATPase in the endoplasmic reticulum. Plant Physiol. 119:1165-1176. Howell, S. H. 2013. Endoplasmic reticulum stress responses in plants. Annu. Rev. Plant Biol. 64:477-499. Jehle, A. K., Fürst, U., Lipschis, M., Albert, M., and Felix, G. 2013. Perception of the novel MAMP eMax from different Xanthomonas species requires the Arabidopsis receptor-like protein ReMAX and the receptor kinase SOBIR. Plant Singal. Behav. 8:e27408. Jiang, R. H., Tyler, B. M., Whisson, S. C., Hardham, A. R., and Govers, F. 2006. Ancient origin of elicitin gene clusters in Phytophthora genomes. Mol. Biol. Evol. 23:338-351. Jing, M., and Wang, Y. 2020. Plant pathogens utilize effectors to hijack the host endoplasmic reticulum as part of their infection strategy. Engineering. 6:500-504. Jones, J. D., and Dangl, J. L. 2006. The plant immune system. Nature 444:323-329. Kamoun, S., Furzer, O., Jones, J. D., Judelson, H. S., Ali, G. S., Dalio, R. J., Roy, S. G., Schena, L., Zambounis, A., and Panabières, F. 2015. The Top 10 oomycete pathogens in molecular plant pathology. Mol. Plant. Pathol. 16:413-434. Kamoun, S., Klucher, K. M., Coffey, M. D., and Tyler, B. M. 1993. A gene encoding a host-specific elicitor protein of Phytophthora parasitica. Mol. Plant Microbe Interact. 6:573-573. Kanyuka, K., and Rudd, J. J. 2019. Cell surface immune receptors: the guardians of the plant’s extracellular spaces. Curr. Opin. Plant. Bio. 50:1-8. Karimi, M., Inzé, D., and Depicker, A. 2002. GATEWAY™ vectors for Agrobacterium-mediated plant transformation. Trends Plant Sci. 7:193-195. Ko, W.-H. 1988. Hormonal heterothallism and homothallism in Phytophthora. Annu. Rev. Phytopathol. 26:57-73. Kong, G., Zhao, Y., Jing, M., Huang, J., Yang, J., Xia, Y., Kong, L., Ye, W., Xiong, Q., and Qiao, Y. 2015. The activation of Phytophthora effector Avr3b by plant cyclophilin is required for the nudix hydrolase activity of Avr3b. PLoS Pathog. 11:e1005139. Doi: https://doi.org/10.1371/journal.ppat.1005139 Lacaze, A., and Joly, D. L. 2020. Structural specificity in plant–filamentous pathogen interactions. Mol. Plant Pathol. 21:1513-1525. Larroque, M., Belmas, E., Martinez, T., Vergnes, S., Ladouce, N., Lafitte, C., Gaulin, E., and Dumas, B. 2013. Pathogen-associated molecular pattern-triggered immunity and resistance to the root pathogen Phytophthora parasitica in Arabidopsis. J. Exp. Bot. 64:3615-3625. Leslie, M. E., Lewis, M. W., Youn, J.-Y., Daniels, M. J., and Liljegren, S. J. 2010. The EVERSHED receptor-like kinase modulates floral organ shedding in Arabidopsis. Development 137:467-476. Li, J., Zhao-Hui, C., Batoux, M., Nekrasov, V., Roux, M., Chinchilla, D., Zipfel, C., and Jones, J. D. 2009. Specific ER quality control components required for biogenesis of the plant innate immune receptor EFR. Proc. Natl. Acad. Sci. U. S. A. 106:15973-15978. Li, Y.-H., Ke, T.-Y., Shih, W.-C., Liou, R.-F., and Wang, C.-W. 2021. NbSOBIR1 partitions into plasma membrane microdomains and binds ER-Localized NbRLP1. Front. Plant Sci.1813. Doi: https://doi.org/10.3389/fpls.2021.721548 Liebrand, T. W., van den Berg, G. C., Zhang, Z., Smit, P., Cordewener, J. H., America, A. H., Sklenar, J., Jones, A. M., Tameling, W. I., and Robatzek, S. 2013. Receptor-like kinase SOBIR1/EVR interacts with receptor-like proteins in plant immunity against fungal infection. Proc. Natl. Acad. Sci. U. S. A. 110:10010-10015. Liebrand, T. W., van den Burg, H. A., and Joosten, M. H. 2014. Two for all: receptor-associated kinases SOBIR1 and BAK1. Trends Plant Sci. 19:123-132. Liu, J. X., and Howell, S. H. 2016. Managing the protein folding demands in the endoplasmic reticulum of plants. New Phytol. 211:418-428. Luo, L. 2012. Plant cytokine or phytocytokine. Plant Signal. Behav. 7:1513-1514. McLellan, H., Boevink, P. C., Armstrong, M. R., Pritchard, L., Gomez, S., Morales, J., Whisson, S. C., Beynon, J. L., and Birch, P. R. 2013. An RxLR effector from Phytophthora infestans prevents re-localisation of two plant NAC transcription factors from the endoplasmic reticulum to the nucleus. PLoS Pathog. 9:e1003670. Doi: https://doi.org/10.1371/journal.ppat.1003670 Mikes, V., Milat, M.-L., Ponchet, M., Panabières, F., Ricci, P., and Blein, J.-P. 1998. Elicitins, proteinaceous elicitors of plant defense, are a new class of sterol carrier proteins. Biochem Biophys. Res. Commun. 245:133-139. Mokryakova, M., Pogorelko, G., Bruskin, S., Piruzian, E., and Abdeeva, I. 2014. The role of peptidyl-prolyl cis/trans isomerase genes of Arabidopsis thaliana in plant defense during the course of Xanthomonas campestris infection. Russ. J. Genet. 50:140-148. Mur, L. A., Kenton, P., Lloyd, A. J., Ougham, H., and Prats, E. 2008. The hypersensitive response; the centenary is upon us but how much do we know? J. Exp. Bot. 59:501-520. Na, R., Yu, D., Qutob, D., Zhao, J., and Gijzen, M. 2013. Deletion of the Phytophthora sojae avirulence gene Avr1d causes gain of virulence on Rps1d. Mol. Plant Microbe Interact. 26:969-976. Nelson, B. K., Cai, X., and Nebenführ, A. 2007. A multicolored set of in vivo organelle markers for co‐localization studies in Arabidopsis and other plants. Plant J. 51:1126-1136. Oldroyd, G. E. 2013. Speak, friend, and enter: signalling systems that promote beneficial symbiotic associations in plants. Nat. Rev. Microbiol. 11:252-263. Panabieres, F., Marais, A., Le Berre, J.-Y., Penot, I., Fournier, D., and Ricci, P. 1995. Characterization of a gene cluster of Phytophthora cryptogea which codes for elicitins, proteins inducing a hypersensitive-like response in tobacco. Mol. Plant Microbe Interact. 8:996-1003. Peng, K.-C., Wang, C.-W., Wu, C.-H., Huang, C.-T., and Liou, R.-F. 2015. Tomato SOBIR1/EVR homologs are involved in elicitin perception and plant defense against the oomycete pathogen Phytophthora parasitica. Mol. Plant Microbe Interact. 28:913-926. Pogorelko, G. V., Mokryakova, M., Fursova, O. V., Abdeeva, I., Piruzian, E. S., and Bruskin, S. A. 2014. Characterization of three Arabidopsis thaliana immunophilin genes involved in the plant defense response against Pseudomonas syringae. Gene 538:12-22. Postma, J., Liebrand, T. W., Bi, G., Evrard, A., Bye, R. R., Mbengue, M., Kuhn, H., Joosten, M. H., and Robatzek, S. 2016. Avr4 promotes Cf‐4 receptor‐like protein association with the BAK1/SERK3 receptor‐like kinase to initiate receptor endocytosis and plant immunity. New Phytol. 210:627-642. Ricci, P., Bonnet, P., Huet, J. C., Sallantin, M., Beauvais-cante, F., Bruneteau, M., Billard, V., Michel, G., and Pernollet, J. C. 1989. Structure and activity of proteins from pathogenic fungi Phytophthora eliciting necrosis and acquired resistance in tobacco. Eur. J. Biochem. 183:555-563. Shan, W., Cao, M., Leung, D., and Tyler, B. M. 2004. The Avr1b locus of Phytophthora sojae encodes an elicitor and a regulator required for avirulence on soybean plants carrying resistance gene Rps1b. Mol. Plant Microbe Interact. 17:394-403. Shao, S., and Hegde, R. S. 2011. Membrane protein insertion at the endoplasmic reticulum. Annu. Rev. Cell Dev. Biol. 27:25. Shi, W. C. 2017. The role of NbRLP1 in response of Nicotiana benthamiana to the elicitin ParA1. Master thesis. National Taiwan University, Taipei, Taiwan. Srivastava, R., Deng, Y., Shah, S., Rao, A. G., and Howell, S. H. 2013. BINDING PROTEIN is a master regulator of the endoplasmic reticulum stress sensor/transducer bZIP28 in Arabidopsis. Plant Cell 25:1416-1429. Stong, R. A., Kolodny, E., Kelsey, R. G., González-Hernández, M., Vivanco, J. M., and Manter, D. K. 2013. Effect of plant sterols and tannins on Phytophthora ramorum growth and sporulation. J. Chem. Ecol. 39:733-743. Stornaiuolo, M., Lotti, L. V., Borgese, N., Torrisi, M.-R., Mottola, G., Martire, G., and Bonatti, S. 2003. KDEL and KKXX retrieval signals appended to the same reporter protein determine different trafficking between endoplasmic reticulum, intermediate compartment, and Golgi complex. Mol. Biol. Cell. 14:889-902. Thomma, B. P., Nürnberger, T., and Joosten, M. H. 2011. Of PAMPs and effectors: the blurred PTI-ETI dichotomy. Plant Cell 23:4-15. Van Der Burgh, A. M., Postma, J., Robatzek, S., and Joosten, M. H. 2019. Kinase activity of SOBIR1 and BAK1 is required for immune signalling. Mol. Plant Pathol. 20:410-422. Voinnet, O., Rivas, S., Mestre, P., and Baulcombe, D. 2003. Retracted: An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus. Plant J. 33:949-956. Wallis, J. G., and Browse, J. 2010. Lipid biochemists salute the genome. Plant J. 61:1092-1106. Wang, P., Hawes, C., and Hussey, P. J. 2017. Plant endoplasmic reticulum–plasma membrane contact sites. Trends Plant Sci. 22:289-297. Wang, P., Hawkins, T. J., Richardson, C., Cummins, I., Deeks, M. J., Sparkes, I., Hawes, C., and Hussey, P. J. 2014. The plant cytoskeleton, NET3C, and VAP27 mediate the link between the plasma membrane and endoplasmic reticulum. Curr. Biol. 24:1397-1405. Wang, Y., Xu, Y., Sun, Y., Wang, H., Qi, J., Wan, B., Ye, W., Lin, Y., Shao, Y., and Dong, S. 2018. Leucine-rich repeat receptor-like gene screen reveals that Nicotiana RXEG1 regulates glycoside hydrolase 12 MAMP detection. Nat. Commun. 9:1-12. Xu, G., Li, S., Xie, K., Zhang, Q., Wang, Y., Tang, Y., Liu, D., Hong, Y., He, C., and Liu, Y. 2012. Plant ERD2‐like proteins function as endoplasmic reticulum luminal protein receptors and participate in programmed cell death during innate immunity. Plant J.72:57-69. Yang, X., Tyler, B. M., and Hong, C. 2017. An expanded phylogeny for the genus Phytophthora. IMA fungus. 8:355. Ye, C., Dickman, M. B., Whitham, S. A., Payton, M., and Verchot, J. 2011. The unfolded protein response is triggered by a plant viral movement protein. Plant Physiol. 156:741-755. Zhang, L., Kars, I., Essenstam, B., Liebrand, T. W., Wagemakers, L., Elberse, J., Tagkalaki, P., Tjoitang, D., van den Ackerveken, G., and van Kan, J. A. 2014. Fungal endopolygalacturonases are recognized as microbe-associated molecular patterns by the Arabidopsis receptor-like protein RESPONSIVENESS TO BOTRYTIS POLYGALACTURONASES1. Plant Physiol. 164:352-364. Zhang, W., Fraiture, M., Kolb, D., Löffelhardt, B., Desaki, Y., Boutrot, F. F., Tör, M., Zipfel, C., Gust, A. A., and Brunner, F. 2013. Arabidopsis receptor-like protein30 and receptor-like kinase suppressor of BIR1-1/EVERSHED mediate innate immunity to necrotrophic fungi. Plant Cell 25:4227-4241. Zheng, X.-y., Zhou, M., Yoo, H., Pruneda-Paz, J. L., Spivey, N. W., Kay, S. A., and Dong, X. 2015. Spatial and temporal regulation of biosynthesis of the plant immune signal salicylic acid. Proc. Natl. Acad. Sci. U. S. A. 112:9166-9173. Zipfel, C. 2014. Plant pattern-recognition receptors. Trends Immunol. 35:345-351. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/85949 | - |
dc.description.abstract | 被微生物侵染時,植物藉由 pattern-recognition receptors (PRRs) 辨識 microbial-associated molecular patterns (MAMPs) 或植物結構被微生物分解後的 damage-associated molecular patterns (DAMPs) 引發 pattern-triggered immunity (PTI)。根據是否含有 kinase domain,PRRs 被分為 receptor-like kinase (RLKs) 和 receptor-like proteins (RLPs)。SOBIR1 (Suppressor of BIR1-1) 是許多 RLP 型 PRRs 啟動植物防禦反應時重要的 adaptor kinase,也被證實參與植物辨識卵菌 elicitins 的過程。先前的研究藉由蛋白免疫沉澱和液相層析串聯質譜分析在圓葉菸草 (Nicotiana benthamiana) 發現多個可能與 SlSOBIR1 具有交互作用的蛋白,本研究深入探討其中一個基因 (定名為N. benthamiana SOBIR1-interacting RLP 2;NbSIR2) 的特性。NbSIR2 包含 817 個胺基酸,具備 signal peptide、leucine rich repeat (LRR) domain,以及穿膜區 (transmembrane domain, TMD) 等保守性構造;穿膜區後還有一小段 cytoplasmic tail。接種疫病菌後,NbSIR2 的表現量顯著上升,並且過表現 NbSIR2 加劇疫病菌 elicitin ParA1 引發的細胞壞疽。蛋白共免疫沉澱實驗證實了 NbSIR2 與 NbSOBIR1 存在交互作用,共軛焦螢光顯微鏡觀察與序列分析結合推斷 NbSIR2 位於植物細胞內質網膜上。SOBIR1 是植物免疫反應的一個關鍵點,本研究對 SOBIR1 調控之免疫反應提供新的研究視野,同時也為植物內質網膜蛋白參與植物的免疫反應提供新的研究方向,其中有更多機制亟待瞭解。 | zh_TW |
dc.description.abstract | When infected by microbial pathogens, plants recognize microbial-associated molecular patterns (MAMPs) or plant-derived damage-associated molecular patterns (DAMPs) through pattern-recognition receptors (PRRs). Depending on the protein structure, PRRs are classified into receptor-like kinase (RLKs) and receptor-like proteins (RLPs), which differ only in the presence of C-terminal kinase domains for RLKs. SOBIR1 (suppressor of BIR1-1) has been identified as an adaptor kinase for various PRRs of the RLP type. As well, it is involved in plant response toward elicitins of Phytophthora, including ParA1 from Phytophthora parasitica. Previous studies based on co-immunoprecipitation coupled by liquid chromatography-tandem mass spectrometry (LC-MS/MS) identified various Nicotiana benthamiana proteins which might interact with SlSOBIR1. In this study, one of these genes, named SOBIR1-interacting N. benthamiana RLP2 (NbSIR2) was characterized for its role in plant response to ParA1. The expression of NbSIR2 is induced upon P. parasitica infection and NbSIR2 overexpression enhanced ParA1-induced necrosis. Co-immunoprecipitation experiment showed that NbSIR2 associated with NbSOBIR1 in planta in an elicitin-independent manner. Furthermore, analyses based on sequence prediction and confocal microscopy indicate NbSIR2 likely locates on the membrane of endoplasmic reticulum (ER). SOBIR1 plays a central role in PTI involving PRRs of the RLP type. This study introduces a new role of NbSIR2 in SOBIR1-mediated plant immunity and also helps to gain new insight into how ER membrane protein might participate in plant immunity. | en |
dc.description.provenance | Made available in DSpace on 2023-03-19T23:30:07Z (GMT). No. of bitstreams: 1 U0001-1209202211581700.pdf: 5800564 bytes, checksum: 06403f828d75e93c6ae2452789c62ee4 (MD5) Previous issue date: 2022 | en |
dc.description.tableofcontents | 一、 前言 1 1. 植物防禦反應 1 1.1 Pattern Triggered Immunity 2 1.2 SOBIR1 3 2. 疫病菌 4 2.1 疫病菌的生活史 5 2.2 疫病菌與植物的交互作用 5 2.3 Elicitin 6 3. 內質網與植物防禦反應 8 4. 研究動機 10 二、 材料與方法 11 1. 序列分析及親緣性分析 11 2. 供試植物與生長條件 11 3. 疫病菌接種實驗 11 4. 植物基因表現量分析 12 4.1 萃取植物 RNA 並製備 cDNA 12 4.2 即時定量聚合酶鏈鎖反應 (quantitative reverse transcriptase PCR, qRT-PCR) 13 5. 製備 ParA1 重組蛋白並處理植物分析其活性 14 6. 植物基因靜默 15 6.1 載體構築 16 6.2 農桿菌感染法 16 7. 過表現 NbSIR2 17 7.1 載體構築 17 7.2 農桿菌感染法 18 8. 以化學冷光法 (Chemiluminescence) 檢測活性氧分子 18 9. NbSIR2 與 NbSOBIR1 交互作用分析 19 9.1 蛋白共免疫沉澱法 (Co-immunoprecipitation, Co-IP) 19 9.1.1 載體構築 19 9.1.2 TAP-tagged 蛋白純化 20 9.2 西方墨點實驗 21 10. 分析 NbSIR2 在植物細胞的分佈情形 22 10.1 基因表現 22 10.2 以雷射掃描式共軛焦螢光顯微鏡進行觀察 23 11. 統計分析方法 23 三、 結果 24 1. NbSIR2 序列分析 24 2. NbSIR2 親緣關係關係分析 24 3. 菸草接種疫病菌後 NbSIR2 表現量上升 25 4. 純化 ParA1 蛋白 26 5. 過表現 NbSIR2 之基因加劇 ParA1 蛋白造成之細胞壞疽 26 6. 靜默 NbSIR2 之基因表現緩解 ParA1 蛋白造成之細胞壞疽 27 7. NbSIR2-GFP 分佈在內質網膜上 28 8. NbSIR2 與 NbSOBIR1 之交互作用 29 9. ParA1 在菸草引發活性氧化物的累積 30 四、 討論 32 1. NbSIR2 位於 ER membrane 32 2. NbSIR2 與 NbSOBIR1 具有交互作用 32 3. NbSIR2 參與 ParA1 引發的免疫反應 33 4. 結語 34 | |
dc.language.iso | zh-TW | |
dc.title | 探討 NbSIR2 在圓葉菸草對疫病菌 ParA1 elicitin 反應中的角色 | zh_TW |
dc.title | The role of NbSIR2 in the response of Nicotiana benthamiana to ParA1 elicitin of Phytophthora parasitica | en |
dc.type | Thesis | |
dc.date.schoolyear | 110-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 鍾嘉綾(Chia-Lin Chung),吳志航(Chih-Hang Wu) | |
dc.subject.keyword | 疫病菌,植物基礎防禦反應,內質網,elicitin,NbSIR2,ParA1,SOBIR1, | zh_TW |
dc.subject.keyword | elicitin,endoplasmic reticulum (ER),NbSIR2,ParA1,phytophthora parasitica,pattern-triggered immunity,SOBIR1, | en |
dc.relation.page | 55 | |
dc.identifier.doi | 10.6342/NTU202203300 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2022-09-22 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 植物病理與微生物學研究所 | zh_TW |
dc.date.embargo-lift | 2022-09-27 | - |
顯示於系所單位: | 植物病理與微生物學系 |
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