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  1. NTU Theses and Dissertations Repository
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請用此 Handle URI 來引用此文件: http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/85798
完整後設資料紀錄
DC 欄位值語言
dc.contributor.advisor季昭華(Chau-Hwa Chi)
dc.contributor.authorWei-Yin Laien
dc.contributor.author賴薇因zh_TW
dc.date.accessioned2023-03-19T23:24:43Z-
dc.date.copyright2022-04-26
dc.date.issued2022
dc.date.submitted2022-04-11
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J., Haynes, E., Gramhofer, M., Stanford, K., Bailey, S., Christman, M., Conley, K., Frasca, S., Ossiboff, R. J., Lobato, D., & Allender, M. C. (2020). Case definition and diagnostic testings for snake fungal disease. Herpetological Review, 50(2), 279-285. Başaran Kahraman, B., Diren Sığırcı, B., Metiner, K., Ak, S., Koenhemsi, L., Or, M. E., Castellá, G., & Abarca, M. L. (2015). Isolation of Chrysosporium guarroi in a Green Iguana (Iguana iguana), in Turkey. Journal of Exotic Pet Medicine, 24(4), 427-429. https://doi.org/10.1053/j.jepm.2015.08.007 Chandler, H. C., Allender, M. C., Stegenga, B. S., Haynes, E., Ospina, E., & Stevenson, D. J. (2019). Ophidiomycosis prevalence in Georgia's eastern indigo snake (Drymarchon couperi) populations. PLoS One, 14(6), e0218351. https://doi.org/10.1371/journal.pone.0218351 Cheatwood, J. L., Jacobson, E. R., May, P. G., Farrell, T. M., Homer, B. L., Samuelson, D. A., & Kimbrough, J. W. (2003). An outbreak of fungal dermatitis and stomatitis in a free-ranging population of pigmy rattlesnakes (Sistrurus miliarius barbouri) in Florida. J Wildl Dis, 39(2), 329-337. https://doi.org/10.7589/0090-3558-39.2.329 Chen, Y. H., Chi, M. J., Sun, P. L., Yu, P. H., Liu, C. H., Cano-Lira, J. F., & Li, W. T. (2020). Histopathology, molecular identification and antifungal susceptibility testing of Nannizziopsis arthrosporioides from a captive cuban rock iguana (Cyclura nubila). Mycopathologia, 185(6), 1005-1012. https://doi.org/10.1007/s11046-020-00481-6 Christman, J. E., Alexander, A. B., Donnelly, K. A., Ossiboff, R. J., Stacy, N. I., Richardson, R. L., Case, J. B., Childress, A. L., & Wellehan, J. F. X. (2020). Clinical manifestation and molecular characterization of a novel member of the Nannizziopsiaceae in a pulmonary granuloma from a Galapagos Tortoise (Chelonoidis nigra). Front Vet Sci, 7, 24. https://doi.org/10.3389/fvets.2020.00024 Diaz-Delgado, J., Marrow, J. C., Flanagan, J. 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Palmerston North New Zealand.
dc.identifier.urihttp://tdr.lib.ntu.edu.tw/jspui/handle/123456789/85798-
dc.description.abstractChrysosporium Anamorph of Nannizziopsis vriesii(CANV)為Nannizziopsis、Ophidiomyces與Paranannizziopsis屬之真菌組成的集合體,造成多種壞死、潰瘍等皮膚病灶,近年來不斷影響圈養與野生爬蟲的健康。本研究針對台灣野生蛇類設置樣區調查,於夜間在樣區內捕捉有明顯皮膚肉眼病灶之個體,並蒐集有皮膚病灶的救傷個體,進行真菌培養與病理診斷,若發現病灶組織培養出目標真菌,且同時在組織下發現同特徵的菌絲與孢子,才定義為感染。本研究共發現1隻紅斑蛇、與1隻王錦蛇感染Ophidiomyces ophiodiicola,1隻中華眼鏡蛇病灶雖有培養出O. ophiodiicola但病理下無真菌蹤跡,以及1隻王錦蛇和1隻青蛇的表皮分離出新種的Nannizziopsis,但病理無感染證據。樣區調查之陽性率0.11%,遠低於過去國外針對野外族群之調查結果。本研究證實臺灣確實存在Ophidiomyces與Nannizziopsis屬之真菌,未來可再針對樣區內無皮膚病灶之個體以棉棒抹取樣本,檢驗是否有無症狀,但PCR陽性之個體存在,並更進一步針對棲地分布侷限(如水蛇、游蛇)及不易偵測(日行性蛇種)進行捕捉調查,以期未來更全面掌握這些真菌在臺灣野生蛇類的狀況。zh_TW
dc.description.abstractChrysosporium Anamorph of Nannizziopsis vriesii (CANV) is a collection of fungi of the genera Nannizziopsis, Ophidiomyces and Paranannizziopsis. CANV has resulted in a variety of skin lesions such as necrosis and ulcers in reptiles and continuously affected both captive and wild reptiles’ health. We investigate wild snakes with skin lesions area from night field survey and rescued individuals in Taiwan. Surgical biopsy on the lesions was performed to collected for fungal culture and histopathological diagnosis. Infection is defined as either Ophidiomyces, Nannizziopsis or Paranannizziopsis species is cultured and found under tissue pathologically at the same time. One red-banded snake and 1 king snake were infected with Ophidiomyces ophiodiicola. One Chinese cobra detected with O. ophiodiicola but without fungal elements pathologically. Novel Nannizziopsis sp. were isolated from the epidermis of one king snake and one green snake, but there was no pathological evidence of infection. Positive rate of the sample area survey was 0.11%, which was far lower than the previous field survey results from abroad. This study confirms that fungi of the genus Ophidiomyces and Nannizziopsis do exist in Taiwan. In the future, collecting skin swabs for PCR from individuals without skin lesions in the sample area would be suggested to test whether there are asymptomatic individuals that are tested positive of the target pathogens. Snakes with limited territory ( e.g. water snakes) and diurnal snake species are suggested to be further surveyed, in order to more comprehensively understand the status of these fungi in wild snakes in Taiwan.en
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dc.description.tableofcontents誌謝 i 摘要 ii Abastract iii 目錄 iv 圖目錄 v 表目錄 v 第一章、 前言 1 第二章、 文獻探討 3 第一節、 Chrysosporium anamorph of Nannizziopsis vriesii簡介 3 第二節、 Ophidiomyces ophiodiicola感染 5 第三節、 Nannizziopsis屬感染 13 第四節、 Paranannizziopsis屬感染 15 第五節、 其他真菌 16 第三章、 研究材料與方法 17 第一節、 實驗採集對象、時間、樣區 17 第二節、 病灶採樣與診斷方法 18 第四章、 結果 20 第一節、 臺灣原生蛇類捕捉調查結果 20 第二節、 病灶採集培養、鑑種與組織病理結果 24 第三節、 Ophidiomyces ophiodiicola與Nannizziopsis屬感染案例 35 第五章、 討論與總結 46 第一節、 樣區內盛行率 46 第二節、 Ophidiomyces與Nannizziopsis陽性個體 49 第三節、 其他種類真菌感染 50 第四節、 總結 51 參考文獻 52
dc.language.isozh-TW
dc.subject蛇類真菌病zh_TW
dc.subject病理學zh_TW
dc.subject真菌培養zh_TW
dc.subject臺灣zh_TW
dc.subject蛇類zh_TW
dc.subjectSnake fungal diseaseen
dc.subjectPathologyen
dc.subjectMycologyen
dc.subjectTaiwanen
dc.subjectSnakesen
dc.title臺灣野生蛇類皮膚病灶真菌調查zh_TW
dc.titleDetection of fungi on dermal lesions of wild snakes in Taiwanen
dc.typeThesis
dc.date.schoolyear110-2
dc.description.degree碩士
dc.contributor.advisor-orcid季昭華(0000-0003-3398-8898)
dc.contributor.oralexamcommittee余品奐(Pin-Huan Yu),孫培倫(Pei-Lun Sun)
dc.contributor.oralexamcommittee-orcid余品奐(0000-0002-3788-030X),孫培倫(0000-0002-9868-4628)
dc.subject.keyword蛇類真菌病,蛇類,臺灣,真菌培養,病理學,zh_TW
dc.subject.keywordSnake fungal disease,Snakes,Taiwan,Mycology,Pathology,en
dc.relation.page59
dc.identifier.doi10.6342/NTU202200681
dc.rights.note同意授權(全球公開)
dc.date.accepted2022-04-11
dc.contributor.author-college獸醫專業學院zh_TW
dc.contributor.author-dept臨床動物醫學研究所zh_TW
dc.date.embargo-lift2022-04-26-
顯示於系所單位:臨床動物醫學研究所

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