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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 鄭尊仁(Tsun-Jen Cheng) | |
dc.contributor.author | Ting-An Lin | en |
dc.contributor.author | 林庭安 | zh_TW |
dc.date.accessioned | 2023-03-19T22:32:23Z | - |
dc.date.copyright | 2022-10-04 | |
dc.date.issued | 2022 | |
dc.date.submitted | 2022-08-24 | |
dc.identifier.citation | 1. 郭子瑄. (2021). 次微米塑膠微粒在小鼠經由腸胃道的吸收分佈及毒性研究. https://doi.org/10.6342/NTU202101281 2. Adeva-Andany, M. M., Pérez-Felpete, N., Fernández-Fernández, C., Donapetry-García, C., & Pazos-García, C. (2016). Liver glucose metabolism in humans. Bioscience reports, 36(6). 3. Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., & Walter, P. (2002). Transport into the cell from the plasma membrane: endocytosis. In Molecular Biology of the Cell. 4th edition. Garland Science. 4. Allen, S., Allen, D., Phoenix, V. R., Le Roux, G., Durántez Jiménez, P., Simonneau, A., Binet, S., & Galop, D. (2019). Atmospheric transport and deposition of microplastics in a remote mountain catchment. Nature Geoscience, 12(5), 339-344. 5. Ashton, K., Holmes, L., & Turner, A. (2010). Association of metals with plastic production pellets in the marine environment. Marine Pollution Bulletin, 60(11), 2050-2055. 6. Banerjee, A., & Shelver, W. L. (2021). Micro-and nanoplastic induced cellular toxicity in mammals: A review. Science of the Total Environment, 755, 142518. 7. Bläsing, M., & Amelung, W. (2018). Plastics in soil: Analytical methods and possible sources. Science of the Total Environment, 612, 422-435. 8. Boursier, J., Mueller, O., Barret, M., Machado, M., Fizanne, L., Araujo‐Perez, F., Guy, C. D., Seed, P. C., Rawls, J. F., & David, L. A. (2016). The severity of nonalcoholic fatty liver disease is associated with gut dysbiosis and shift in the metabolic function of the gut microbiota. Hepatology, 63(3), 764-775. 9. Brennecke, D., Duarte, B., Paiva, F., Caçador, I., & Canning-Clode, J. (2016). Microplastics as vector for heavy metal contamination from the marine environment. Estuarine, Coastal and Shelf Science, 178, 189-195. 10. Cai, L., Wang, J., Peng, J., Tan, Z., Zhan, Z., Tan, X., & Chen, Q. (2017). Characteristic of microplastics in the atmospheric fallout from Dongguan city, China: preliminary research and first evidence. Environmental Science and Pollution Research, 24(32), 24928-24935. https://link.springer.com/content/pdf/10.1007/s11356-017-0116-x.pdf 11. Castro, F., Cardoso, A. P., Gonçalves, R. M., Serre, K., & Oliveira, M. J. (2018). Interferon-gamma at the crossroads of tumor immune surveillance or evasion. Frontiers in immunology, 9, 847. 12. Cattley, R. C., & Cullen, J. M. (2013). Liver and gall bladder. In Haschek and Rousseaux's handbook of toxicologic pathology (pp. 1509-1566). Elsevier. 13. Chain, E. P. o. C. i. t. F. (2016). Presence of microplastics and nanoplastics in food, with particular focus on seafood. Efsa Journal, 14(6), e04501. 14. Chawla, L. S., Bellomo, R., Bihorac, A., Goldstein, S. L., Siew, E. D., Bagshaw, S. M., Bittleman, D., Cruz, D., Endre, Z., & Fitzgerald, R. L. (2017). Acute kidney disease and renal recovery: consensus report of the Acute Disease Quality Initiative (ADQI) 16 Workgroup. Nature Reviews Nephrology, 13(4), 241-257. 15. Chen, Y., Zhang, H.-S., Fong, G.-H., Xi, Q.-L., Wu, G.-H., Bai, C.-G., Ling, Z.-Q., Fan, L., Xu, Y.-M., & Qin, Y.-Q. (2015). PHD3 stabilizes the tight junction protein occludin and protects intestinal epithelial barrier function. Journal of Biological Chemistry, 290(33), 20580-20589. 16. Cho, I., Yamanishi, S., Cox, L., Methé, B. A., Zavadil, J., Li, K., Gao, Z., Mahana, D., Raju, K., & Teitler, I. (2012). Antibiotics in early life alter the murine colonic microbiome and adiposity. Nature, 488(7413), 621-626. 17. Choi, Y. J., Park, J. W., Lim, Y., Seo, S., & Hwang, D. Y. (2021). In vivo impact assessment of orally administered polystyrene nanoplastics: biodistribution, toxicity, and inflammatory response in mice. Nanotoxicology, 1-19. 18. Cole, M., Lindeque, P., Halsband, C., & Galloway, T. S. (2011). Microplastics as contaminants in the marine environment: A review [Review]. Marine Pollution Bulletin, 62(12), 2588-2597. https://doi.org/10.1016/j.marpolbul.2011.09.025 19. Cox, K. D., Covernton, G. A., Davies, H. L., Dower, J. F., Juanes, F., & Dudas, S. E. (2019). Human consumption of microplastics. Environmental Science & Technology, 53(12), 7068-7074. 20. da Silva Brito, W. A., Mutter, F., Wende, K., Cecchini, A. L., Schmidt, A., & Bekeschus, S. (2022). Consequences of nano and microplastic exposure in rodent models: the known and unknown. Particle and Fibre Toxicology, 19(1), 1-24. 21. Daniel, D. B., Ashraf, P. M., Thomas, S. N., & Thomson, K. T. (2021). Microplastics in the edible tissues of shellfishes sold for human consumption [Article]. Chemosphere, 264, 9, Article 128554. https://doi.org/10.1016/j.chemosphere.2020.128554 22. Del Rio, D., Stewart, A. J., & Pellegrini, N. (2005). A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutrition, Metabolism and Cardiovascular Diseases, 15(4), 316-328. 23. Delzenne, N. M., Knudsen, C., Beaumont, M., Rodriguez, J., Neyrinck, A. M., & Bindels, L. B. (2019). Contribution of the gut microbiota to the regulation of host metabolism and energy balance: a focus on the gut–liver axis. Proceedings of the Nutrition Society, 78(3), 319-328. 24. Deng, Y., Zhang, Y., Lemos, B., & Ren, H. (2017). Tissue accumulation of microplastics in mice and biomarker responses suggest widespread health risks of exposure. Scientific Reports, 7(1), 1-10. 25. Dharmaraj, S., Ashokkumar, V., Hariharan, S., Manibharathi, A., Show, P. L., Chong, C. T., & Ngamcharussrivichai, C. (2021). The COVID-19 pandemic face mask waste: A blooming threat to the marine environment [Review]. Chemosphere, 272, 20, Article 129601. https://doi.org/10.1016/j.chemosphere.2021.129601 26. Di Ciaula, A., Baj, J., Garruti, G., Celano, G., De Angelis, M., Wang, H. H., Di Palo, D. M., Bonfrate, L., Wang, D. Q., & Portincasa, P. (2020). Liver steatosis, gut-liver axis, microbiome and environmental factors. A never-ending bidirectional cross-talk. Journal of clinical medicine, 9(8), 2648. 27. Ding, J., Huang, Y., Liu, S., Zhang, S., Zou, H., Wang, Z., Zhu, W., & Geng, J. (2020). Toxicological effects of nano-and micro-polystyrene plastics on red tilapia: are larger plastic particles more harmless? Journal of Hazardous Materials, 396, 122693. 28. Ding, Y., Zhang, R., Li, B., Du, Y., Li, J., Tong, X., Wu, Y., Ji, X., & Zhang, Y. (2021). Tissue distribution of polystyrene nanoplastics in mice and their entry, transport, and cytotoxicity to GES-1 cells. Environmental Pollution, 280, 116974. 29. Dris, R., Gasperi, J., Mirande, C., Mandin, C., Guerrouache, M., Langlois, V., & Tassin, B. (2017). A first overview of textile fibers, including microplastics, in indoor and outdoor environments. Environmental Pollution, 221, 453-458. https://www.sciencedirect.com/science/article/pii/S0269749116312325?via%3Dihub 30. Dris, R., Gasperi, J., Rocher, V., Saad, M., Renault, N., & Tassin, B. (2015). Microplastic contamination in an urban area: a case study in Greater Paris [Article]. Environmental Chemistry, 12(5), 592-599. https://doi.org/10.1071/en14167 31. Dris, R., Gasperi, J., Saad, M., Mirande, C., & Tassin, B. (2016). Synthetic fibers in atmospheric fallout: A source of microplastics in the environment? [Article]. Marine Pollution Bulletin, 104(1-2), 290-293. https://doi.org/10.1016/j.marpolbul.2016.01.006 32. Du, F., Cai, H., Zhang, Q., Chen, Q., & Shi, H. (2020). Microplastics in take-out food containers. Journal of Hazardous Materials, 399, 122969. https://www.sciencedirect.com/science/article/pii/S0304389420309584?via%3Dihub 33. Eerkes-Medrano, D., Thompson, R. C., & Aldridge, D. C. (2015). Microplastics in freshwater systems: a review of the emerging threats, identification of knowledge gaps and prioritisation of research needs. Water research, 75, 63-82. 34. Eriksen, M., Lebreton, L. C. M., Carson, H. S., Thiel, M., Moore, C. J., Borerro, J. C., Galgani, F., Ryan, P. G., & Reisser, J. (2014). Plastic Pollution in the World's Oceans: More than 5 Trillion Plastic Pieces Weighing over 250,000 Tons Afloat at Sea [Article]. Plos One, 9(12), 15, Article e111913. https://doi.org/10.1371/journal.pone.0111913 35. Eriksen, M., Maximenko, N., Thiel, M., Cummins, A., Lattin, G., Wilson, S., Hafner, J., Zellers, A., & Rifman, S. (2013). Plastic pollution in the South Pacific subtropical gyre [Article]. Marine Pollution Bulletin, 68(1-2), 71-76. https://doi.org/10.1016/j.marpolbul.2012.12.021 36. Fan, X. P., Wei, X. J., Hu, H. L., Zhang, B. Y., Yang, D. Q., Du, H. N., Zhu, R. J., Sun, X. T., Oh, Y. R., & Gu, N. (2022). Effects of oral administration of polystyrene nanoplastics on plasma glucose metabolism in mice [Article]. Chemosphere, 288, 10, Article 132607. https://doi.org/10.1016/j.chemosphere.2021.132607 37. Geyer, R., Jambeck, J. R., & Law, K. L. (2017). Production, use, and fate of all plastics ever made [Article]. Science Advances, 3(7), 5, Article e1700782. https://doi.org/10.1126/sciadv.1700782 38. Haddad, J. J. (2002). Antioxidant and prooxidant mechanisms in the regulation of redox (y)-sensitive transcription factors. Cellular signalling, 14(11), 879-897. 39. Hernandez, L. M., Xu, E. G., Larsson, H. C., Tahara, R., Maisuria, V. B., & Tufenkji, N. (2019). Plastic teabags release billions of microparticles and nanoparticles into tea. Environmental Science & Technology, 53(21), 12300-12310. 40. Hillery, A., Jani, P., & Florence, A. (1994). Comparative, quantitative study of lymphoid and non-lymphoid uptake of 60 nm polystyrene particles. Journal of drug targeting, 2(2), 151-156. 41. Horton, A. A., Walton, A., Spurgeon, D. J., Lahive, E., & Svendsen, C. (2017). Microplastics in freshwater and terrestrial environments: Evaluating the current understanding to identify the knowledge gaps and future research priorities [Review]. Science of the Total Environment, 586, 127-141. https://doi.org/10.1016/j.scitotenv.2017.01.190 42. Horvatits, T., Tamminga, M., Liu, B., Sebode, M., Carambia, A., Fischer, L., Püschel, K., Huber, S., & Fischer, E. K. (2022). Microplastics detected in cirrhotic liver tissue. EBioMedicine, 82, 104147. 43. Hu, C.-W., Chang, Y.-J., Chen, J.-L., Hsu, Y.-W., & Chao, M.-R. (2018). Sensitive detection of 8-nitroguanine in DNA by chemical derivatization coupled with online solid-phase extraction LC-MS/MS. Molecules, 23(3), 605. 44. Huang, D., Zhang, Y., Long, J., Yang, X., Bao, L., Yang, Z., Wu, B., Si, R., Zhao, W., & Peng, C. (2022). Polystyrene microplastic exposure induces insulin resistance in mice via dysbacteriosis and pro-inflammation. Science of the Total Environment, 155937. 45. Im, C., Kim, H., Zaheer, J., Kim, J. Y., Lee, Y.-J., Kang, C. M., & Kim, J. S. (2022). PET tracing of biodistribution for orally administered 64Cu-labeled polystyrene in mice. Journal of Nuclear Medicine, 63(3), 461-467. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8978192/pdf/jnm120.256982.pdf 46. Jani, P., McCarthy, D., & Florence, A. (1992). Nanosphere and microsphere uptake via Peyer's patches: observation of the rate of uptake in the rat after a single oral dose. International journal of pharmaceutics, 86(2-3), 239-246. 47. Jin, Y., Lu, L., Tu, W., Luo, T., & Fu, Z. (2019). Impacts of polystyrene microplastic on the gut barrier, microbiota and metabolism of mice. Science of the Total Environment, 649, 308-317. 48. Jin, Y., Wu, S., Zeng, Z., & Fu, Z. (2017). Effects of environmental pollutants on gut microbiota. Environmental Pollution, 222, 1-9. 49. Johnson, A. C., & Zager, R. A. (2018). Mechanisms underlying increased TIMP2 and IGFBP7 urinary excretion in experimental AKI. Journal of the American Society of Nephrology, 29(8), 2157-2167. https://jasn.asnjournals.org/content/jnephrol/29/8/2157.full.pdf 50. Kern, D. G., Kuhn III, C., Ely, E. W., Pransky, G. S., Mello, C. J., Fraire, A. E., & Müller, J. (2000). Flock worker's lung: broadening the spectrum of clinicopathology, narrowing the spectrum of suspected etiologies. Chest, 117(1), 251-259. https://www.sciencedirect.com/science/article/pii/S0012369215309569?via%3Dihub 51. Kim, S. R., Lee, Y.-h., Lee, S.-G., Kang, E. S., Cha, B.-S., Kim, J.-H., & Lee, B.-W. (2016). Urinary N-acetyl-β-D-glucosaminidase, an early marker of diabetic kidney disease, might reflect glucose excursion in patients with type 2 diabetes. Medicine, 95(27). 52. Kimura, S. (2018). Molecular insights into the mechanisms of M-cell differentiation and transcytosis in the mucosa-associated lymphoid tissues. Anatomical science international, 93(1), 23-34. 53. Kirstein, I. V., Hensel, F., Gomiero, A., Iordachescu, L., Vianello, A., Wittgren, H. B., & Vollertsen, J. (2021). Drinking plastics?–Quantification and qualification of microplastics in drinking water distribution systems by µFTIR and Py-GCMS. Water research, 188, 116519. https://www.sciencedirect.com/science/article/pii/S004313542031054X?via%3Dihub 54. Kirstein, I. V., Kirmizi, S., Wichels, A., Garin-Fernandez, A., Erler, R., Löder, M., & Gerdts, G. (2016). Dangerous hitchhikers? Evidence for potentially pathogenic Vibrio spp. on microplastic particles. Marine environmental research, 120, 1-8. 55. Klein, M., & Fischer, E. K. (2019). Microplastic abundance in atmospheric deposition within the Metropolitan area of Hamburg, Germany [Article]. Science of the Total Environment, 685, 96-103. https://doi.org/10.1016/j.scitotenv.2019.05.405 56. Kwo, P. Y., Cohen, S. M., & Lim, J. K. (2017). ACG clinical guideline: evaluation of abnormal liver chemistries. Official journal of the American College of Gastroenterology| ACG, 112(1), 18-35. 57. Law, K. L., Moret-Ferguson, S., Maximenko, N. A., Proskurowski, G., Peacock, E. E., Hafner, J., & Reddy, C. M. (2010). Plastic Accumulation in the North Atlantic Subtropical Gyre [Article]. Science, 329(5996), 1185-1188. https://doi.org/10.1126/science.1192321 58. Lee, H., Kunz, A., Shim, W. J., & Walther, B. A. (2019). Microplastic contamination of table salts from Taiwan, including a global review. Scientific Reports, 9(1), 1-9. 59. Li, D., Shi, Y., Yang, L., Xiao, L., Kehoe, D. K., Gun’ko, Y. K., Boland, J. J., & Wang, J. J. (2020). Microplastic release from the degradation of polypropylene feeding bottles during infant formula preparation. Nature Food, 1(11), 746-754. 60. Liao, C.-P., Chiu, C.-C., & Huang, H.-W. (2021). Assessment of microplastics in oysters in coastal areas of Taiwan. Environmental Pollution, 286, 117437. https://www.sciencedirect.com/science/article/pii/S0269749121010198?via%3Dihub 61. Lippi, G., Plebani, M., Di Somma, S., & Cervellin, G. (2011). Hemolyzed specimens: a major challenge for emergency departments and clinical laboratories. Critical reviews in clinical laboratory sciences, 48(3), 143-153. 62. Liu, G., Wang, J., Wang, M., Ying, R., Li, X., Hu, Z., & Zhang, Y. (2022). Disposable plastic materials release microplastics and harmful substances in hot water. Science of the Total Environment, 818, 151685. 63. Loomba, R., Seguritan, V., Li, W., Long, T., Klitgord, N., Bhatt, A., Dulai, P. S., Caussy, C., Bettencourt, R., & Highlander, S. K. (2017). Gut microbiome-based metagenomic signature for non-invasive detection of advanced fibrosis in human nonalcoholic fatty liver disease. Cell metabolism, 25(5), 1054-1062. e1055. 64. Lu, L., Wan, Z. Q., Luo, T., Fu, Z. W., & Jin, Y. X. (2018). Polystyrene microplastics induce gut microbiota dysbiosis and hepatic lipid metabolism disorder in mice [Article]. Science of the Total Environment, 631-632, 449-458. https://doi.org/10.1016/j.scitotenv.2018.03.051 65. Lusher, A. L., Tirelli, V., O'Connor, I., & Officer, R. (2015). Microplastics in Arctic polar waters: the first reported values of particles in surface and sub-surface samples [Article]. Scientific Reports, 5, 9, Article 14947. https://doi.org/10.1038/srep14947 66. Maass, S., Daphi, D., Lehmann, A., & Rillig, M. C. (2017). Transport of microplastics by two collembolan species [Article]. Environmental Pollution, 225, 456-459. https://doi.org/10.1016/j.envpol.2017.03.009 67. Machado, A. A. D., Kloas, W., Zarfl, C., Hempel, S., & Rillig, M. C. (2018). Microplastics as an emerging threat to terrestrial ecosystems [Article]. Global Change Biology, 24(4), 1405-1416. https://doi.org/10.1111/gcb.14020 68. Mason, S. A., Welch, V. G., & Neratko, J. (2018). Synthetic polymer contamination in bottled water. Frontiers in chemistry, 407. 69. Mastrangelo, G., Fedeli, U., Fadda, E., Milan, G., Turato, A., & Pavanello, S. (2003). Lung cancer risk in workers exposed to poly (vinyl chloride) dust: a nested case-referent study. Occupational and environmental medicine, 60(6), 423-428. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1740548/pdf/v060p00423.pdf 70. McDermid, K. J., & McMullen, T. L. (2004). Quantitative analysis of small-plastic debris on beaches in the Hawaiian archipelago [Article]. Marine Pollution Bulletin, 48(7-8), 790-794. https://doi.org/10.1016/j.marpolbul.2003.10.017 71. Mead, J. R., Irvine, S. A., & Ramji, D. P. (2002). Lipoprotein lipase: structure, function, regulation, and role in disease. Journal of molecular medicine, 80(12), 753-769. 72. Meng, X., Zhang, J., Wang, W., Gonzalez-Gil, G., Vrouwenvelder, J. S., & Li, Z. (2022). Effects of nano-and microplastics on kidney: Physicochemical properties, bioaccumulation, oxidative stress and immunoreaction. Chemosphere, 288, 132631. https://www.sciencedirect.com/science/article/pii/S0045653521031039?via%3Dihub 73. Mintenig, S., Löder, M., Primpke, S., & Gerdts, G. (2019). Low numbers of microplastics detected in drinking water from ground water sources. Science of the Total Environment, 648, 631-635. https://www.sciencedirect.com/science/article/pii/S0048969718331425?via%3Dihub 74. Moore, C. J., Moore, S. L., Leecaster, M. K., & Weisberg, S. B. (2001). A comparison of plastic and plankton in the North Pacific central gyre [Article]. Marine Pollution Bulletin, 42(12), 1297-1300. https://doi.org/10.1016/s0025-326x(01)00114-x 75. Moore, C. J., Moore, S. L., Weisberg, S. B., Lattin, G. L., & Zellers, A. F. (2002). A comparison of neustonic plastic and zooplankton abundance in southern California's coastal waters [Article]. Marine Pollution Bulletin, 44(10), 1035-1038, Article Pii s0025-326x(02)00150-9. https://doi.org/10.1016/s0025-326x(02)00150-9 76. Mori, K., Lee, H. T., Rapoport, D., Drexler, I. R., Foster, K., Yang, J., Schmidt-Ott, K. M., Chen, X., Li, J. Y., & Weiss, S. (2005). Endocytic delivery of lipocalin-siderophore-iron complex rescues the kidney from ischemia-reperfusion injury. The Journal of clinical investigation, 115(3), 610-621. 77. Mu, Y., Sun, J., Li, Z., Zhang, W., Liu, Z., Li, C., Peng, C., Cui, G., Shao, H., & Du, Z. (2022). Activation of pyroptosis and ferroptosis is involved in the hepatotoxicity induced by polystyrene microplastics in mice. Chemosphere, 291, 132944. 78. Murphy, F., Ewins, C., Carbonnier, F., & Quinn, B. (2016). Wastewater treatment works (WwTW) as a source of microplastics in the aquatic environment. Environmental Science & Technology, 50(11), 5800-5808. 79. Nguyen, P., Leray, V., Diez, M., Serisier, S., Bloc’h, J. L., Siliart, B., & Dumon, H. (2008). Liver lipid metabolism. Journal of animal physiology and animal nutrition, 92(3), 272-283. 80. Nizzetto, L., Futter, M., & Langaas, S. (2016). Are agricultural soils dumps for microplastics of urban origin? In: ACS Publications. 81. Oh, D.-J. (2020). A long journey for acute kidney injury biomarkers. Renal failure, 42(1), 154-165. 82. Oßmann, B. E., Sarau, G., Holtmannspötter, H., Pischetsrieder, M., Christiansen, S. H., & Dicke, W. (2018). Small-sized microplastics and pigmented particles in bottled mineral water. Water research, 141, 307-316. 83. Ostermann, M., Zarbock, A., Goldstein, S., Kashani, K., Macedo, E., Murugan, R., Bell, M., Forni, L., Guzzi, L., & Joannidis, M. (2020). Recommendations on acute kidney injury biomarkers from the acute disease quality initiative consensus conference: a consensus statement. JAMA network open, 3(10), e2019209-e2019209. https://jamanetwork.com/journals/jamanetworkopen/articlepdf/2771386/ostermann_2020_cs_200002_1604501146.08266.pdf 84. Patel, R. P., Shah, P., Barve, K., Patel, N., & Gandhi, J. (2019). Peyer’s Patch: Targeted Drug Delivery for Therapeutics Benefits. Novel Drug Delivery Technologies, 121-149. 85. Paul, M. B., Stock, V., Cara-Carmona, J., Lisicki, E., Shopova, S., Fessard, V., Braeuning, A., Sieg, H., & Böhmert, L. (2020). Micro-and nanoplastics–current state of knowledge with the focus on oral uptake and toxicity. Nanoscale Advances, 2(10), 4350-4367. 86. Powell, J. J., Faria, N., Thomas-McKay, E., & Pele, L. C. (2010). Origin and fate of dietary nanoparticles and microparticles in the gastrointestinal tract. Journal of autoimmunity, 34(3), J226-J233. https://www.sciencedirect.com/science/article/pii/S0896841109001462?via%3Dihub 87. Qiao, J., Chen, R., Wang, M., Bai, R., Cui, X., Liu, Y., Wu, C., & Chen, C. (2021). Perturbation of gut microbiota plays an important role in micro/nanoplastics-induced gut barrier dysfunction. Nanoscale, 13(19), 8806-8816. https://pubs.rsc.org/en/content/articlepdf/2021/nr/d1nr00038a 88. Ranjan, V. P., Joseph, A., & Goel, S. (2021). Microplastics and other harmful substances released from disposable paper cups into hot water [Article]. Journal of Hazardous Materials, 404, 12, Article 124118. https://doi.org/10.1016/j.jhazmat.2020.124118 89. Revel, M., Châtel, A., & Mouneyrac, C. (2018). Micro (nano) plastics: A threat to human health? Current Opinion in Environmental Science & Health, 1, 17-23. 90. Rillig, M. C., Ziersch, L., & Hempel, S. (2017). Microplastic transport in soil by earthworms [Article]. Scientific Reports, 7, 6, Article 1362. https://doi.org/10.1038/s41598-017-01594-7 91. Rodrigues, J. P., Duarte, A. C., Santos-Echeandía, J., & Rocha-Santos, T. (2019). Significance of interactions between microplastics and POPs in the marine environment: a critical overview. TrAC Trends in Analytical Chemistry, 111, 252-260. 92. Sampson, M. J., Gopaul, N., Davies, I. R., Hughes, D. A., & Carrier, M. J. (2002). Plasma F2 isoprostanes: direct evidence of increased free radical damage during acute hyperglycemia in type 2 diabetes. Diabetes care, 25(3), 537-541. 93. Sangkham, S., Faikhaw, O., Munkong, N., Sakunkoo, P., Arunlertaree, C., Chavali, M., Mousazadeh, M., & Tiwari, A. (2022). A review on microplastics and nanoplastics in the environment: Their occurrence, exposure routes, toxic studies, and potential effects on human health. Marine Pollution Bulletin, 181, 113832. 94. Saptarshi, S. R., Duschl, A., & Lopata, A. L. (2013). Interaction of nanoparticles with proteins: relation to bio-reactivity of the nanoparticle. Journal of nanobiotechnology, 11(1), 1-12. 95. Scaldaferri, F., Pizzoferrato, M., Gerardi, V., Lopetuso, L., & Gasbarrini, A. (2012). The gut barrier: new acquisitions and therapeutic approaches. Journal of clinical gastroenterology, 46, S12-S17. 96. Schwabl, P., Köppel, S., Königshofer, P., Bucsics, T., Trauner, M., Reiberger, T., & Liebmann, B. (2019). Detection of various microplastics in human stool: a prospective case series. Annals of internal medicine, 171(7), 453-457. 97. Shen, R., Yang, K., Cheng, X., Guo, C., Xing, X., Sun, H., Liu, D., Liu, X., & Wang, D. (2022). Accumulation of polystyrene microplastics induces liver fibrosis by activating cGAS/STING pathway. Environmental Pollution, 300, 118986. https://www.sciencedirect.com/science/article/pii/S0269749122002007?via%3Dihub 98. Shi, C., Han, X., Guo, W., Wu, Q., Yang, X., Wang, Y., Tang, G., Wang, S., Wang, Z., & Liu, Y. (2022). Disturbed Gut-Liver axis indicating oral exposure to polystyrene microplastic potentially increases the risk of insulin resistance. Environment international, 164, 107273. 99. Shi, C. Z., Han, X. H., Guo, W., Wu, Q., Yang, X. X., Wang, Y. Y., Tang, G., Wang, S. H., Wang, Z. N., Liu, Y. Q., Li, M., Lv, M. L., Guo, Y. H., Li, Z. K., Li, J. Y., Shi, J. B., Qu, G. B., & Jiang, G. B. (2022). Disturbed Gut-Liver axis indicating oral exposure to polystyrene microplastic potentially increases the risk of insulin resistance [Article]. Environment international, 164, 13, Article 107273. https://doi.org/10.1016/j.envint.2022.107273 100. Shoeb, M., H Ansari, N., K Srivastava, S., & V Ramana, K. (2014). 4-Hydroxynonenal in the pathogenesis and progression of human diseases. Current medicinal chemistry, 21(2), 230-237. 101. Simundic, A.-M., Baird, G., Cadamuro, J., Costelloe, S. J., & Lippi, G. (2020). Managing hemolyzed samples in clinical laboratories. Critical reviews in clinical laboratory sciences, 57(1), 1-21. 102. Stärkel, P., & Schnabl, B. (2016). Bidirectional communication between liver and gut during alcoholic liver disease. Seminars in liver disease, 103. Steinmetz, Z., Wollmann, C., Schaefer, M., Buchmann, C., David, J., Tröger, J., Muñoz, K., Frör, O., & Schaumann, G. E. (2016). Plastic mulching in agriculture. Trading short-term agronomic benefits for long-term soil degradation? Science of the Total Environment, 550, 690-705. 104. Stock, V., Böhmert, L., Lisicki, E., Block, R., Cara-Carmona, J., Pack, L. K., Selb, R., Lichtenstein, D., Voss, L., & Henderson, C. J. (2019). Uptake and effects of orally ingested polystyrene microplastic particles in vitro and in vivo. Archives of Toxicology, 93(7), 1817-1833. https://link.springer.com/content/pdf/10.1007/s00204-019-02478-7.pdf 105. Stock, V., Bohmert, L., Lisicki, E., Block, R., Cara-Carmona, J., Pack, L. K., Selb, R., Lichtenstein, D., Voss, L., Henderson, C. J., Zabinsky, E., Sieg, H., Braeuning, A., & Lampen, A. (2019). Uptake and effects of orally ingested polystyrene microplastic particles in vitro and in vivo [Article]. Archives of Toxicology, 93(7), 1817-1833. https://doi.org/10.1007/s00204-019-02478-7 106. Stock, V., Fahrenson, C., Thuenemann, A., Dönmez, M. H., Voss, L., Böhmert, L., Braeuning, A., Lampen, A., & Sieg, H. (2020). Impact of artificial digestion on the sizes and shapes of microplastic particles. Food and chemical toxicology, 135, 111010. 107. Streetz, K., Luedde, T., Manns, M., & Trautwein, C. (2000). Interleukin 6 and liver regeneration. Gut, 47(2), 309-312. 108. Sundbæk, K. B., Koch, I. D. W., Villaro, C. G., Rasmussen, N. S., Holdt, S. L., & Hartmann, N. B. (2018). Sorption of fluorescent polystyrene microplastic particles to edible seaweed Fucus vesiculosus. Journal of Applied Phycology, 30(5), 2923-2927. 109. Sykes, B. D. (2007). Urine stability for metabolomic studies: effects of preparation and storage. Metabolomics, 3(1), 19-27. 110. Tacke, F., Luedde, T., & Trautwein, C. (2009). Inflammatory pathways in liver homeostasis and liver injury. Clinical reviews in allergy & immunology, 36(1), 4-12. 111. Tan, H., Yue, T., Xu, Y., Zhao, J., & Xing, B. (2020). Microplastics reduce lipid digestion in simulated human gastrointestinal system. Environmental Science & Technology, 54(19), 12285-12294. 112. Tessari, P., Coracina, A., Cosma, A., & Tiengo, A. (2009). Hepatic lipid metabolism and non-alcoholic fatty liver disease. Nutrition, Metabolism and Cardiovascular Diseases, 19(4), 291-302. 113. Tripathi, A., Debelius, J., Brenner, D. A., Karin, M., Loomba, R., Schnabl, B., & Knight, R. (2018). The gut–liver axis and the intersection with the microbiome. Nature reviews Gastroenterology & hepatology, 15(7), 397-411. 114. Valko, M., Leibfritz, D., Moncol, J., Cronin, M. T., Mazur, M., & Telser, J. (2007). Free radicals and antioxidants in normal physiological functions and human disease. The international journal of biochemistry & cell biology, 39(1), 44-84. 115. Velzeboer, I., Kwadijk, C., & Koelmans, A. (2014). Strong sorption of PCBs to nanoplastics, microplastics, carbon nanotubes, and fullerenes. Environmental Science & Technology, 48(9), 4869-4876. 116. Vital, S., Cardoso, C., Avio, C., Pittura, L., Regoli, F., & Bebianno, M. (2021). Do microplastic contaminated seafood consumption pose a potential risk to human health? Marine Pollution Bulletin, 171, 112769. https://www.sciencedirect.com/science/article/pii/S0025326X21008031?via%3Dihub 117. Wagner, M., Scherer, C., Alvarez-Muñoz, D., Brennholt, N., Bourrain, X., Buchinger, S., Fries, E., Grosbois, C., Klasmeier, J., & Marti, T. (2014). Microplastics in freshwater ecosystems: what we know and what we need to know. Environmental Sciences Europe, 26(1), 1-9. 118. Walczak, A. P., Hendriksen, P. J., Woutersen, R. A., van der Zande, M., Undas, A. K., Helsdingen, R., van den Berg, H. H., Rietjens, I. M., & Bouwmeester, H. (2015). Bioavailability and biodistribution of differently charged polystyrene nanoparticles upon oral exposure in rats. Journal of Nanoparticle Research, 17(5), 1-13. 119. Walkinshaw, C., Lindeque, P. K., Thompson, R., Tolhurst, T., & Cole, M. (2020). Microplastics and seafood: lower trophic organisms at highest risk of contamination [Article]. Ecotoxicology and Environmental Safety, 190, 14, Article 110066. https://doi.org/10.1016/j.ecoenv.2019.110066 120. Wang, W., Ge, J., & Yu, X. (2020). Bioavailability and toxicity of microplastics to fish species: a review. Ecotoxicology and Environmental Safety, 189, 109913. 121. Wang, Y.-L., Lee, Y.-H., Hsu, Y.-H., Chiu, I.-J., Huang, C. C.-Y., Huang, C.-C., Chia, Z.-C., Lee, C.-P., Lin, Y.-F., & Chiu, H.-W. (2021). The kidney-related effects of polystyrene microplastics on human kidney proximal tubular epithelial cells HK-2 and male C57BL/6 mice. Environmental health perspectives, 129(5), 057003. 122. Wendel, A. (1981). [44] Glutathione peroxidase. In Methods in enzymology (Vol. 77, pp. 325-333). Elsevier. 123. Wieland, A., Frank, D., Harnke, B., & Bambha, K. (2015). Systematic review: microbial dysbiosis and nonalcoholic fatty liver disease. Alimentary pharmacology & therapeutics, 42(9), 1051-1063. 124. Wright, S. L., & Kelly, F. J. (2017). Plastic and human health: a micro issue? Environmental Science & Technology, 51(12), 6634-6647. 125. Wu, L. L., Chiou, C.-C., Chang, P.-Y., & Wu, J. T. (2004). Urinary 8-OHdG: a marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetics. Clinica chimica acta, 339(1-2), 1-9. 126. Zhang, J.-M., & An, J. (2007). Cytokines, inflammation and pain. International anesthesiology clinics, 45(2), 27. 127. Zhang, N., Li, Y. B., He, H. R., Zhang, J. F., & Ma, G. S. (2021). You are what you eat: Microplastics in the feces of young men living in Beijing. Science of the Total Environment, 767, 144345. https://www.sciencedirect.com/science/article/pii/S0048969720378761?via%3Dihub 128. Zhao, L., Shi, W., Hu, F., Song, X., Cheng, Z., & Zhou, J. (2021). Prolonged oral ingestion of microplastics induced inflammation in the liver tissues of C57BL/6J mice through polarization of macrophages and increased infiltration of natural killer cells. Ecotoxicology and Environmental Safety, 227, 112882. 129. Zheng, H., Wang, J., Wei, X., Chang, L., & Liu, S. (2021). Proinflammatory properties and lipid disturbance of polystyrene microplastics in the livers of mice with acute colitis. Science of the Total Environment, 750, 143085. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/84913 | - |
dc.description.abstract | 塑膠製品已是人類生活不可或缺的一部份,然而過度使用,導致環境內積累大量的塑膠垃圾卻難以被分解。目前研究已知塑膠產品會透過物理、化學以及生物性的方式降解成塑膠碎片,小於5毫米的塑膠碎片被定義為微米級塑膠微粒,介於1毫米到5毫米之間的塑膠碎片被稱為次微米級塑膠微粒。塑膠微粒可藉由攝入、吸入以及皮膚接觸進入體內,目前研究認為攝入是人類主要的暴露途徑。 先前我們實驗室研究經胃管灌食暴露次微米級塑膠微粒四週後,發現跟控制組相比,暴露組小鼠24小時排尿量減少、腎臟發炎反應以及肝臟重量減少。此外,近期研究指出塑膠微粒暴露後會導致肝臟脂質代謝異常、胰島素調節失常以及天門冬胺酸轉胺酵素 (Aspartate Transaminase, AST) 與丙胺酸轉胺酵素 (Alanine aminotransferase, ALT) 指數上升。少數論文也發現塑膠微粒對腎臟的毒性,包括腎臟重量下降、促發炎因子含量上升、腎小管損傷等。然而,至今塑膠微粒引起肝毒性及腎毒性的確切機制仍不明確,此外上述研究多為急性或亞急性研究,所以本研究探討亞慢性暴露塑膠微粒後引起的肝、腎毒性,進一步瞭解腸道菌群與肝毒性之間的關聯,亦會分析微米與次微米級塑膠微粒對肝、腎臟毒性之差異。 所有的動物實驗皆通過國立臺灣大學實驗動物照護及使用委員會審查(同意書編號:20201287)。本研究使用次微米(0.5 微米)以及微米(5微米)之尼羅紅螢光聚苯乙烯塑膠微粒 (Polystyrene microplastic, PS-MPs),每週胃管灌食小鼠兩次,每次0.3毫克,共12週。暴露之前、中以及後利用代謝籠收集小鼠的尿液以及糞便共5次。在實驗終點,小鼠將斷食4小時,並且用愛福寧麻醉犧牲小鼠,收集周邊血液、肝臟、腎臟以及腸道內容物以 -80度保存待後續分析。將代謝籠收集之尿液進行基本檢測,以及用Elisa kit檢測腎臟早期損傷生物標誌物,包括N-acetyl-β-D-glucosaminidase (NAG)、Kidney Injury Molecule-1 (KIM-1)、Neutrophil gelatinase-associated lipocalin (NGAL) 以及Tissue inhibitor of metalloproteinases-2 (TIMP-2) 與Insulin like Growth Factor Binding Protein 7 (IGFBP7)。血液進行血清生化檢測,檢測項目包括,白蛋白 (Albumin, ALB)、ALT、AST、總膽固醇 (Total cholesterol, TCH)、三酸甘油酯 (Triglyceride, TG)、血糖 (Glucose, GLU)、高密度脂蛋白 (High-density lipoprotein, HDL)、低密度脂蛋白 (Low-density lipoprotein, LDL)、血清尿素氮 (Blood urea nitrogen, BUN) 與血清肌肝酸 (Serum creatinine, sCr)。肝臟與腎臟切片以H&E染色與Oil red O染色進行病理分析。以Elisa kit 檢測腎臟與肝臟消化液中促發炎因子,包括Interferon-γ (IFN-γ)、Interleukin-1β (IL-1β)、Interleukin-6 (IL-6) 及Tumor necrosis factor-α (TNF-α)。以Elisa kit 檢測肝臟與腎臟消化液中氧化壓力指標,包括Superoxide Dismutase (SOD)、8-Oxo-2'-deoxyguanosine (8-OhdG)、8-nitroguanine (8-NO2Gua)、4-hydroxy-2-nonenal-mercapturic acid (HNE-MA) 以及Prostaglandin F2α (PGF2a)。此外,全景組織細胞分析儀 (TissueFAXS) 與流式細胞儀 (Flow cytometry, FCM),為檢測 PS-MPs進入肝臟與腎臟後的分佈。最後使用盲腸內容物進行 16S rRNA 基因測序分析檢測菌群豐度改變。 對於肝臟的研究結果表示,0.5 µm暴露組在TissueFAXS發現PS-MPs 累積,在 FCM 檢測下,平均有 105 顆 PS-MPs 累積在 1 克的肝臟組織中。此外,血清檢測肝功能指標 AST 與 ALT 以及代謝指標 TG 及 GLU ,在 0.5 µm暴露組顯著高於控制組。生物指標氧化壓力包括 SOD 與 HNE-MA 及促發炎因子 IL-6 在 0.5 µm 暴露組顯著高於控制組。而後根據組織病理學的檢測,發現經過Oil red O 染色後脂肪油滴表現量在0.5 µm暴露組顯著高於控制組。5 µm暴露組發現肝臟重量指數顯著高於控制組,在血清生化檢測,HDL顯著高於控制組。腎臟結果雖未觀察到PS-MPs的累積或是組織學變化,然而在氧化壓力 SOD 以及促發炎因子 IL-6 與IFN-γ,0.5 µm暴露組表現量顯著高於控制組。此外,相關性分析結果顯示,放線菌門以及脫鐵桿菌門的改變對 ALT、AST 與 TG 變化有高度正相關。上述實驗結果表明,亞慢性暴露次微米PS-MPs後,可能藉由進入小鼠肝臟內累積或改變腸道菌群豐度,直接與間接的引起小鼠肝臟氧化壓力、發炎反應以及血糖與脂質的代謝異常。腎臟中雖未觀察到塑膠微粒的累積,但仍可在次微米級塑膠微粒暴露組別發現氧化壓力與發炎反應的增加。 總體來說,經過12週重複暴露 PS-MPs 後,次微米 PS-MPs 對肝臟與腎臟的毒性反應大於微米級,且對肝臟代謝功能有較大的毒性,後續應繼續探討次微米塑膠微粒長期暴露對肝臟代謝機制的影響。 | zh_TW |
dc.description.abstract | Plastic products have become useful in many aspects of human life because of their excellent chemical and physical properties. However, over-used plastic products and slow biodegradation rate, resulting in the accumulation the plastic litters in the environment. Practically, plastic products are degraded into microplastic through chemical and physical degradation. Microplastic and submicron plastic are a kind of plastic particle with a diameter of 1 µm to 5 mm and less than 1 µm, respectively. These plastic particle fragments can enter the human body through ingestion, inhalation and dermal contact. Ingestion is considered the major exposure route. Our previous research investigated the distribution and toxicity of submicron plastic particles in mice by oral gavage. We found that 24-hour urine was decreased, inflammatory factors of kidney were increased and liver weight was decreased as compared to control group after 4 weeks exposure. Recent studies have indicated that microplastics could accumulate in liver tissue, and alter the composition and diversity of gut microbiota, thus causing hepatic lipid disorder, insulin resistance, and abnormal liver enzymes test Aspartate Transaminase (AST), Alanine aminotransferase (ALT). Furthermore, a few articles also found some toxicological effects on kidney in mice, including decreased kidney weight, increased proinflammatory cytokines, and tubular injury and albumin leakage in urine sample. However, the exact mechanisms remain unclear. In addition, most studies are acute or subacute toxicity tests. Therefore, I would like to investigate the nephrotoxicity and hepatotoxicity induced by subchronic exposure to polystyrene microplastics in mice, and further study the association between hepatotoxicity and gut microbiota. The toxicity between micron and submicron plastic particles will also be compared. All animal procedures were approved by the Institutional Animal Care and Use Committee (IACUC Approval No: 20201287). In my study, 6 weeks old C57BL/6 female mice were administered with 0.3 mg of 0.5 µm (submicron) and 5 µm (micron) Nile red fluorescent polystyrene microplastics (PS-MPs) twice a week for 12 weeks by oral gavage. Mouse urine were collected in metabolic cages for 24 hours before, during and after the exposure experiment. At the end of the experiment, all mice were fasted for 4 hours, anesthetized with isoflurane and sacrificed. After sacrificing, the peripheral blood, liver tissue, kidney tissue and cecum content were collected and stored in -80 ℃ refrigerator. Urine were analyzed for basic urinalysis and early molecular biomarkers for kidney dysfunction by Elisa kit, including N-acetyl-β-D-glucosaminidase (NAG), Kidney Injury Molecule-1 (KIM-1), Neutrophil gelatinase-associated lipocalin (NGAL), and Tissue inhibitor of metalloproteinases-2 (TIMP-2) and Insulin like Growth Factor Binding Protein 7 (IGFBP7). Serum biochemistry were used to test kidney and liver damage by microplastic, including Albumin (ALB), ALT, AST, Total cholesterol (TCH), Triglyceride (TG), Glucose (GLU), High-density lipoprotein (HDL), Low-density lipoprotein (LDL), Blood urea nitrogen (BUN), and serum creatinine (sCr). Liver and kidney tissue were stained for light microscopy examination and Oil red O test for pathological changes. Furthermore, proinflammatory factors of Interferon-γ (IFN-γ), Interleukin-1β (IL-1β), Interleukin-6 (IL-6), Tumor necrosis factor α (TNFα) and markers of oxidative stress of Superoxide Dismutase (SOD), 8-Oxo-2'-deoxyguanosine (8-OhdG), 8-nitroguanine (8-NO2Gua), 4-hydroxy-2-nonenal-mercapturic acid (HNE-MA) and Prostaglandin F2α (PGF2a) were measured by ELISA kits. TissueFAXS and Flow Cytometry (FCM) were carried out for realizing the distribution and determining the number of particles in the liver and kidney, respectively. To determine the main constituents of the gut microbiome, cecum content were processed with 16S rRNA gene sequencing analysis, and colon content. The study showed 0.5 µm PS-MPs could accumulate in the liver section of exposure group. On average, liver tissue have 105 particles/g accumulated. Furthermore, we found AST, ALT, TG and GLU level were significantly higher than control group in serum biochemistry analysis. Markers of Oxidative stress and proinflammation factors analysis in liver, we found SOD, HNE-MA level and IL-6 level of 0.5 µm PS-MPs exposure group were significantly higher than the control group. The result of pathological examination showed that the expression of lipid droplets in 0.5 µm PS-MPs exposure group were significantly higher than control group after Oil red O staining. Although no accumulation of 0.5 µm PS-MPs or histological changes were observed in the kidney, the SOD level and IL-6, IFN-γ level were significantly higher in the 0.5 µm PS-MPs exposure group than in the control group in kidney. The 5 µm PS-MPs exposure group found that the liver weight index was significantly higher than that of the control group, and in serum biochemical tests, HDL was significantly higher than that of the control group. In addition, correlation analysis indicated that the alterations of Actinobacteria and Deferriobacteria were positively correlatied with the changes of ALT, AST and TG level in serum. The results above indicated that subchronic exposure to submicron PS-MPs may directly and indirectly induced oxidative stress, increased inflammatory cytokines, insulin resistance and lipid metabolism disorder by PS-MPs accumulation in liver and the altered gut microbiota in mice. Although we did not observe PS-MPs in kidney, the oxidative stress and inflammation cytokines were increased after exposure to submicron microplastics. After continuous exposure to PS-MPs for 12 weeks, submicron PS-MPs can cause severe toxicity effects than micron PS-MPs in liver and kidney. Long-term effects of submicron plastics on mice liver are worth noting in the future. | en |
dc.description.provenance | Made available in DSpace on 2023-03-19T22:32:23Z (GMT). No. of bitstreams: 1 U0001-2208202220511400.pdf: 4640989 bytes, checksum: 0bd87a8b42fcf8c0d89b6533785b604b (MD5) Previous issue date: 2022 | en |
dc.description.tableofcontents | 致謝 i 中文摘要 ii 英文摘要 iv 第一章 前言與研究目的 1 1.1 前言 1 1.2 研究目的 2 第二章 文獻回顧 3 2.1 塑膠微粒的來源及分佈 3 2.2 塑膠微粒的暴露 6 2.2.1 攝入 6 2.2.2 吸入與皮膚接觸 7 2.3 塑膠微粒的毒理動力學 9 2.3.1 吸收 9 2.3.2 分佈 10 2.3.3 代謝與排泄 11 2.4 塑膠微粒的健康效應 13 2.5 塑膠微粒與肝臟毒性 14 2.6 塑膠微粒與腎臟毒性 18 2.7 腸道菌群與肝臟毒性 20 2.7.1 腸-肝軸線 20 2.7.2 塑膠微粒與腸道菌群 21 第三章 材料與方法 22 3.1 實驗流程與架構 22 3.2 實驗動物 24 3.3 聚苯乙烯塑膠微粒 25 3.3.1 微粒的特性 25 3.3.2 暴露溶液製備 27 3.4 暴露方法 29 3.5 實驗動物之檢體收集 30 3.5.1 尿液收集 30 3.5.2 犧牲方式與檢體前處理 31 3.6 實驗動物之檢體分析 33 3.6.1 尿液檢體分析 33 3.6.2 血清檢體分析 35 3.6.3 肝臟、腎臟中微粒檢測 35 3.6.4 肝臟、腎臟生物指標檢測 38 3.6.5 腸道菌群檢測 42 3.6.6 組織病理檢測 42 3.7 統計方法 44 第四章 結果 45 4.1 聚苯乙烯塑膠微粒 45 4.1.1 微粒的特性 45 4.1.2 微粒的數目濃度 45 4.1.3 微粒的螢光強度 46 4.2 實驗動物生理指標 47 4.2.1 體重變化 47 4.2.2 飲水量與排尿量 47 4.2.3 組織重量變化 48 4.3 尿液分析 49 4.4 血清生化分析 50 4.5 組織內微粒的分佈 51 4.5.1 微粒的回收率 51 4.5.2 微粒在組織的分佈 51 4.6 肝臟與腎臟的生物指標 53 4.6.1 氧化壓力 53 4.6.2 促發炎因子 53 4.7 組織病理 55 4.7.1 H&E 染色 55 4.7.2 Oil red O 染色 55 4.8 腸道菌群 57 4.9 相關性分析 58 4.9.1 腸道菌與生物指標相關性分析 58 第五章 討論 59 5.1 塑膠微粒對小鼠生理指標影響 60 5.2 塑膠微粒在肝臟與腎臟的累積 61 5.3 塑膠微粒對於肝臟生物指標的影響 63 5.4 塑膠微粒對於腎臟生物指標的影響 66 5.5 塑膠微粒導致腸道菌群改變對肝臟的影響 68 5.6 研究限制與建議 69 第六章 結論 70 第七章 參考資料 71 | |
dc.language.iso | zh-TW | |
dc.title | 亞慢性暴露微米與次微米聚苯乙烯塑膠微粒對小鼠肝毒性與腎毒性研究 | zh_TW |
dc.title | Hepatotoxicity and Nephrotoxicity induced by subchronic exposure to polystyrene microplastics in mice | en |
dc.type | Thesis | |
dc.date.schoolyear | 110-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蘇大成(Ta-Chen Su),陳達夫(Ta-Fu Chen),吳焜裕(Kuen-Yuh Wu) | |
dc.subject.keyword | 塑膠微粒,胃管灌食,流式細胞儀,肝毒性,腎毒性,氧化壓力,發炎因子,代謝異常, | zh_TW |
dc.subject.keyword | microplastics,oral gavage,flow cytometry,hepatotoxicity,nephrotoxicity,oxidative stress,cytokine,metabolic disorder, | en |
dc.relation.page | 132 | |
dc.identifier.doi | 10.6342/NTU202202669 | |
dc.rights.note | 同意授權(限校園內公開) | |
dc.date.accepted | 2022-08-25 | |
dc.contributor.author-college | 公共衛生學院 | zh_TW |
dc.contributor.author-dept | 環境與職業健康科學研究所 | zh_TW |
dc.date.embargo-lift | 2025-01-01 | - |
顯示於系所單位: | 環境與職業健康科學研究所 |
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