請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/8367
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 黃鵬鵬(Pung-Pung Hwang) | |
dc.contributor.author | Yu-Hsin Tang | en |
dc.contributor.author | 唐玉欣 | zh_TW |
dc.date.accessioned | 2021-05-20T00:52:53Z | - |
dc.date.available | 2023-07-29 | |
dc.date.available | 2021-05-20T00:52:53Z | - |
dc.date.copyright | 2020-09-24 | |
dc.date.issued | 2020 | |
dc.date.submitted | 2020-07-29 | |
dc.identifier.citation | Allard, J. B., Duan, C. (2018). IGF-Binding Proteins: Why Do They Exist and Why Are There So Many? Front Endocrinol 117. Allen, P. J., Weihrauch, D., Grandmaison, V., Dasiewicz, P., Peake, S. J., Anderson, W. G. (2011). The influence of environmental calcium concentrations on calcium flux, compensatory drinking and epithelial calcium channel expression in a freshwater cartilaginous fish. J Exp Biol 214, 996-1006. Baxter, R. C. (2014). IGF binding proteins in cancer: mechanistic and clinical insights. Nat Rev Cancer 14, 329-341. Breves, J. P., Fujimoto, C. K., Phipps-Costin, S. K., Einarsdottir, I. E., Bjornsson, B. T., McCormick, S. D. (2017). Variation in branchial expression among insulin-like growth-factor binding proteins (igfbps) during Atlantic salmon smoltification and seawater exposure. BMC Physiol 17. Cao, Y. B., Chen, X. Q., Wang, S., Chen, X. C., Wang, Y. X., Chang, J. P., Du, J. Z. (2009). Growth hormone and insulin-like growth factor of naked carp (Gymnocypris przewalskii) in Lake Qinghai: expression in different water environments. Gen Comp Endocrinol 161, 400-406. Carboni, J. M., Wittman, M., Yang, Z., Lee, F., Greer, A., Hurlburt, W., Gottardis, M. M. (2009). BMS-754807, a small molecule inhibitor of insulin-like growth factor-1R/IR. Mol Cancer Ther 8, 3341-3349. Clemmons, D. R. (2001). Use of mutagenesis to probe IGF-binding protein structure/function relationships. Endocr Rev 22, 800-817. Clemmons, D. R. (2016). Role of IGF Binding Proteins in Regulating Metabolism. Trends Endocrinol Metab 27, 375-391. Cowley, A. W., Jr., Yang, C., Kumar, V., Lazar, J., Jacob, H., Geurts, A. M., Liang, M. (2016). Pappa2 is linked to salt-sensitive hypertension in Dahl S rats. Physiol Genomic 48, 62-72. Dai, W., Bai, Y., Hebda, L., Zhong, X., Liu, J., Kao, J., Duan, C. (2014). Calcium deficiency-induced and TRP channel-regulated IGF1R-PI3K-Akt signaling regulates abnormal epithelial cell proliferation. Cell Death Differ 21, 568-581. Dai, W., Kamei, H., Zhao, Y., Ding, J., Du, Z., Duan, C. (2010). Duplicated zebrafish insulin-like growth factor binding protein-5 genes with split functional domains: evidence for evolutionarily conserved IGF binding, nuclear localization, and transactivation activity. FASEB J 24, 2020-2029. Daza, D. O., Sundstrom, G., Bergqvist, C. A., Duan, C., Larhammar, D. (2011). Evolution of the insulin-like growth factor binding protein (IGFBP) family. Endocrinology 152, 2278-2289. Deane, E. E., Kelly, S. P., Lo, C. K., Woo, N. Y. (1999). Effects of GH, prolactin and cortisol on hepatic heat shock protein 70 expression in a marine teleost Sparus sarba. J Endocrinol 161, 413-421. Denley, A., Cosgrove, L. J., Booker, G. W., Wallace, J. C., Forbes, B. E. (2005). Molecular interactions of the IGF system. Cytokine Growth Factor Rev 16, 421-439. Duan, C., Plisetskaya, E. M. (1993). Nutritional regulation of insulin-like growth factor-I mRNA expression in salmon tissues. J Endocrinol 139, 243-252. Duan, C., Ren, H., Gao, S. (2010). Insulin-like growth factors (IGFs), IGF receptors, and IGF-binding proteins: roles in skeletal muscle growth and differentiation. Gen Comp Endocrinol 167, 344-351. Duan, C., Xu, Q. (2005). Roles of insulin-like growth factor (IGF) binding proteins in regulating IGF actions. Gen Comp Endocrinol 142, 44-52. Evans, D. H., Piermarini, P. M., Choe, K. P. (2005). The multifunctional fish gill: dominant site of gas exchange, osmoregulation, acid-base regulation, and excretion of nitrogenous waste. Physiol Rev 85, 97-177. Firth, S. M., Baxter, R. C. (2002). Cellular actions of the insulin-like growth factor binding proteins. Endocr Rev 23, 824-854. Garcia de la Serrana, D., Macqueen, D. J. (2018). Insulin-Like Growth Factor-Binding Proteins of Teleost Fishes. Front Endocrinol 9, 80. Hakuno, F., Takahashi, S. I. (2018). IGF1 receptor signaling pathways. J Mol Endocrinol 61, 69-86. Hanukoglu, I., Hanukoglu, A. (2016). Epithelial sodium channel (ENaC) family: Phylogeny, structure-function, tissue distribution, and associated inherited diseases. Gene 579, 95-132. Hiroi, J., Kaneko, T., Tanaka, M. (1999). In vivo sequential changes in chloride cell morphology in the yolk-sac membrane of mozambique tilapia (Oreochromis mossambicus) embryos and larvae during seawater adaptation. J Exp Biol 202, 3485-3495. Hiroi, J., McCormick, S. D., Ohtani-Kaneko, R., Kaneko, T. (2005). Functional classification of mitochondrion-rich cells in euryhaline Mozambique tilapia (Oreochromis mossambicus) embryos, by means of triple immunofluorescence staining for Na+/K+-ATPase, Na+/K+/2Cl- cotransporter and CFTR anion channel. J Exp Biol 208, 2023-2036. Hiroi, J., Yasumasu, S., McCormick, S. D., Hwang, P. P., Kaneko, T. (2008). Evidence for an apical Na-Cl cotransporter involved in ion uptake in a teleost fish. J Exp Biol 211, 2584-2599. Hirose, S., Kaneko, T., Naito, N., Takei, Y. (2003). Molecular biology of major components of chloride cells. Comp Biochem Physiol B Biochem Mol Biol 136, 593-620. Hoeflich, A., Wu, M., Mohan, S., Foll, J., Wanke, R., Froehlich, T., Wolf, E. (1999). Overexpression of insulin-like growth factor-binding protein-2 in transgenic mice reduces postnatal body weight gain. Endocrinology 140, 5488-5496. Hoenderop, J. G., Nilius, B., Bindels, R. J. (2005). Calcium absorption across epithelia. Physiol Rev 85, 373-422. Holick, M. F. (2007). Vitamin D deficiency. N Engl J Med 357, 266-281. Holzman, J. L., Liu, L., Duke, B. J., Kemendy, A. E., Eaton, D. C. (2007). Transactivation of the IGF-1R by aldosterone. Am J Physiol Renal Physiol 292, 1219-1228. Hootman, S. R., Philpott, C. W. (1980). Accessory cells in teleost branchial epithelium. Am J Physiol 238, 199-206. Hsu, H. H., Lin, L. Y., Tseng, Y. C., Horng, J. L., Hwang, P. P. (2014). A new model for fish ion regulation: identification of ionocytes in freshwater- and seawater-acclimated medaka (Oryzias latipes). Cell Tissue Res 357, 225-243. Hung, G. Y., Wu, C. L., Chou, Y. L., Chien, C. T., Horng, J. L., Lin, L. Y. (2019). Cisplatin exposure impairs ionocytes and hair cells in the skin of zebrafish embryos. Aquat Toxicol 209, 168-177. Hwang, P. P., Hirano, R. (1985). Effects of Environmental Salinity on Intercellular Organization and Junctional Structure of Chloride Cells in Early Stages of Teleost Development. J Exp Zool 236, 115-126. Hwang, P. P., Lee, T. H. (2007). New insights into fish ion regulation and mitochondrion-rich cells. Comp. Biochem Physiol A Mol Integr Physiol 148, 479-497. Hwang, P. P., Lee, T. H., Lin, L. Y. (2011). Ion regulation in fish gills: recent progress in the cellular and molecular mechanisms. Am J Physiol Regul Integr Comp Physiol 301, 28-47. Hwang, P. P., Lin, L. Y. (2013). Gill ionic transport, acid-base regulation, and nitrogen excretion. The physiology of fishes, edn 4, 205-233. Eds D. H. Evans., J. B. Claiborne. Boca Raton: CRC Press. Ilatovskaya, D. V., Levchenko, V., Brands, M. W., Pavlov, T. S., Staruschenko, A. (2015). Cross-talk between insulin and IGF-1 receptors in the cortical collecting duct principal cells: implication for ENaC-mediated Na+ reabsorption. Am J Physiol Renal Physiol 308, 713-719. Imai, Y., Busby, W. H., Jr., Smith, C. E., Clarke, J. B., Garmong, A. J., Horwitz, G. D., Clemmons, D. R. (1997). Protease-resistant form of insulin-like growth factor-binding protein 5 is an inhibitor of insulin-like growth factor-I actions on porcine smooth muscle cells in culture. J Clin Invest 100, 2596-2605. Inokuchi, M., Hiroi, J., Watanabe, S., Lee, K. M., Kaneko, T. (2008). Gene expression and morphological localization of NHE3, NCC and NKCC1 a in branchial mitochondria-rich cells of Mozambique tilapia (Oreochromis mossambicus) acclimated to a wide range of salinities. Comp Biochem Physiol A Mol Integr Physiol 151, 151-158. Inokuchi, M., Nakamura, M., Miyanishi, H., Hiroi, J., Kaneko, T. (2017). Functional classification of gill ionocytes and spatiotemporal changes in their distribution after transfer from seawater to freshwater in Japanese seabass. J Exp Biol 220, 4720-4732. Jepsen, M. R., Kloverpris, S., Mikkelsen, J. H., Pedersen, J. H., Fuchtbauer, E. M., Laursen, L. S., Oxvig, C. (2015). Stanniocalcin-2 inhibits mammalian growth by proteolytic inhibition of the insulin-like growth factor axis. J Biol Chem 290, 3430-3439. Jones, J. I., Clemmons, D. R. (1995). Insulin-like growth factors and their binding proteins: biological actions. Endocr Rev 16, 3-34. Khanal, R. C., Nemere, I. (2008). Regulation of intestinal calcium transport. Annu Rev Nutr 28, 179-196. Kloverpris, S., Mikkelsen, J. H., Pedersen, J. H., Jepsen, M. R., Laursen, L. S., Petersen, S. V., Oxvig, C. (2015). Stanniocalcin-1 Potently Inhibits the Proteolytic Activity of the Metalloproteinase Pregnancy-associated Plasma Protein-A. J Biol Chem 29, 21915-21924. Kusakabe, M., Ishikawa, A., Ravinet, M., Yoshida, K., Makino, T., Toyoda, A., Kitano, J. (2017). Genetic basis for variation in salinity tolerance between stickleback ecotypes. Mol Ecol 26, 304-319. Laursen, L. S., Overgaard, M. T., Soe, R., Boldt, H. B., Sottrup-Jensen, L., Giudice, L. C., Oxvig, C. (2001). Pregnancy-associated plasma protein-A (PAPP-A) cleaves insulin-like growth factor binding protein (IGFBP)-5 independent of IGF: implications for the mechanism of IGFBP-4 proteolysis by PAPP-A. FEBS Let 504, 36-40. Liao, B. K., Deng, A. N., Chen, S. C., Chou, M. Y., Hwang, P. P. (2007). Expression and water calcium dependence of calcium transporter isoforms in zebrafish gill mitochondrion-rich cells. BMC Genomics 8, 354. Liebert, A. M., Schreck, C. B. (2006). Effects of acute stress on osmoregulation, feed intake, IGF-1, and cortisol in yearling steelhead trout (Oncorhynchus mykiss) during seawater adaptation. Gen Comp Endocrinol 148, 195-202. Lin, C. C., Lin, L. Y., Hsu, H. H., Thermes, V., Prunet, P., Horng, J. L., Hwang, P. P. (2012). Acid secretion by mitochondrion-rich cells of medaka (Oryzias latipes) acclimated to acidic freshwater. Am J Physiol Regul Integr Comp Physiol 302, 283-291. Lin, C. H., Hwang, P. P. (2016). The Control of Calcium Metabolism in Zebrafish (Danio rerio). Int J Mol Sci 17, 1783. Liu, C., Li, S., Noer, P. R., Kjaer-Sorensen, K., Juhl, A. K., Goldstein, A., Duan, C. (2020). The metalloproteinase Papp-aa controls epithelial cell quiescence-proliferation transition. Elife 9, e52322. Macqueen, D. J., Garcia de la Serrana, D., Johnston, I. A. (2013). Evolution of ancient functions in the vertebrate insulin-like growth factor system uncovered by study of duplicated salmonid fish genomes. Mol Biol Evol 30, 1060-1076. Macqueen, D. J., Johnston, I. A. (2014). A well-constrained estimate for the timing of the salmonid whole genome duplication reveals major decoupling from species diversification. Proc Biol Sci 281, 2881. Mancera, J. M., McCormick, S. D. (1998). Evidence for growth hormone/insulin-like growth factor I axis regulation of seawater acclimation in the euryhaline teleost Fundulus heteroclitus. Gen Comp Endocrinol 111, 103-112. Marshall, W. S., Cozzi, R. R. F., Spieker, M. (2017). WNK1 and p38-MAPK distribution in ionocytes and accessory cells of euryhaline teleost fish implies ionoregulatory function. Biol Open 6, 956-966. Maures, T. J., Duan, C. (2002). Structure, developmental expression, and physiological regulation of zebrafish IGF binding protein-1. Endocrinology 143, 2722-2731. McCormick, S. D., Dickhoff, W. W., Duston, J., Nishioka, R. S., Bern, H. A. (1991). Developmental differences in the responsiveness of gill Na+, K+-ATPase to cortisol in salmonids. Gen Comp Endocrinol 84, 308-317. McCormick, S. D., Regish, A. M., Christensen, A. K., Bjornsson, B. T. (2013). Differential regulation of sodium-potassium pump isoforms during smolt development and seawater exposure of Atlantic salmon. J Exp Biol 216, 1142-1151. Overgaard, M. T., Boldt, H. B., Laursen, L. S., Sottrup-Jensen, L., Conover, C. A., Oxvig, C. (2001). Pregnancy-associated plasma protein-A2 (PAPP-A2), a novel insulin-like growth factor-binding protein-5 proteinase. J Biol Chem 276, 21849-21853. Oxvig, C. (2015). The role of PAPP-A in the IGF system: location, location, location. J Cell Commun Signal 9, 177-187. Pelis, R. M., McCormick, S. D. (2001). Effects of growth hormone and cortisol on Na+-K+-2Cl- cotransporter localization and abundance in the gills of Atlantic salmon. Gen Comp Endocrinol 124, 134-143. Pellissier, T., Al Nafea, H., Good, S. V. (2018). Divergence of insulin superfamily ligands, receptors and Igf binding proteins in marine versus freshwater stickleback: Evidence of selection in known and novel genes. Comp Biochem Physiol Part D Genomics Proteomics 25, 53-61. Perrot, V., Moiseeva, E. B., Gozes, Y., Chan, S. J., Funkenstein, B. (2000). Insulin-like growth factor receptors and their ligands in gonads of a hermaphroditic species, the gilthead seabream (Sparus aurata): expression and cellular localization. Biol Reprod 63, 229-241. Rahman, M. S., Thomas, P. (2017). Molecular and biochemical responses of hypoxia exposure in Atlantic croaker collected from hypoxic regions in the northern Gulf of Mexico. PLoS One 12, e0184341. Reindl, K. M., Sheridan, M. A. (2012). Peripheral regulation of the growth hormone-insulin-like growth factor system in fish and other vertebrates. Comp. Biochem Physiol A Mol Integr Physiol 163, 231-245 Reinecke, M. (2010). Influences of the environment on the endocrine and paracrine fish growth hormone-insulin-like growth factor-I system. J Fish Biol 76, 1233-1254. Reinecke, M., Bjornsson, B. T., Dickhoff, W. W., McCormick, S. D., Navarro, I., Power, D. M., Gutierrez, J. (2005). Growth hormone and insulin-like growth factors in fish: where we are and where to go. Gen Comp Endocrinol 142, 20-24. Schneider, M. R., Wolf, E., Hoeflich, A., Lahm, H. (2002). IGF-binding protein-5: flexible player in the IGF system and effector on its own. J Endocrinol 172, 423-440. Seidelin, M., Madsen, S. S. (1999). Endocrine control of Na+, K+-ATPase and chloride cell development in brown trout (Salmo trutta): interaction of insulin-like growth factor-I with prolactin and growth hormone. J Endocrinol 162, 127-135. Seidelin, M., Madsen, S. S., Byrialsen, A., Kristiansen, K. (1999). Effects of insulin-like growth factor-I and cortisol on Na+, K+-ATPase expression in osmoregulatory tissues of brown trout (Salmo trutta). Gen Comp Endocrinol 113, 331-342. Shahsavarani, A., McNeill, B., Galvez, F., Wood, C. M., Goss, G. G., Hwang, P. P., Perry, S. F. (2006). Characterization of a branchial epithelial calcium channel (ECaC) in freshwater rainbow trout (Oncorhynchus mykiss). J Exp Biol 209, 1928-1943. Shamblott, M. J., Chen, T. T. (1992). Identification of a second insulin-like growth factor in a fish species. Proc Natl Acad Sci U S A 89, 8913-8917. Shen, W. P., Horng, J. L., Lin, L. Y. (2011). Functional plasticity of mitochondrion-rich cells in the skin of euryhaline medaka larvae (Oryzias latipes) subjected to salinity changes. Am J Physiol Regul Integr Comp Physiol 300, 858-868. Shepherd, B. S., Drennon, K., Johnson, J., Nichols, J. W., Playle, R. C., Singer, T. D., Vijayan, M. M. (2005). Salinity acclimation affects the somatotropic axis in rainbow trout. Am J Physiol Regul Integr Comp Physiol 288, 1385-1395. Shepherd, B. S., Sakamoto, T., Nishioka, R. S., Richman, N. H., 3rd, Mori, I., Madsen, S. S., Grau, E. G. (1997). Somatotropic actions of the homologous growth hormone and prolactins in the euryhaline teleost, the tilapia, Oreochromis mossambicus. Proc Natl Acad Sci U S A 94, 2068-2072. Shimasaki, S., Ling, N. (1991). Identification and molecular characterization of insulin-like growth factor binding proteins (IGFBP-1, -2, -3, -4, -5 and -6). Prog Growth Factor Res 3, 243-266. Siwanowicz, I., Popowicz, G. M., Wisniewska, M., Huber, R., Kuenkele, K. P., Lang, K., Holak, T. A. (2005). Structural basis for the regulation of insulin-like growth factors by IGF binding proteins. Structure 13, 155-167. Skou, J. C., Esmann, M. (1992). The Na+, K+-ATPase. J Bioenerg Biomembr 24, 249-261. Taguchi, A., White, M. F. (2008). Insulin-like signaling, nutrient homeostasis, and life span. Annu Rev Physiol 70, 191-212. Tang, Y. L., Shepherd, B. S., Nichols, A. J., Dunham, R., Chen, T. T. (2001). Influence of environmental salinity on messenger RNA levels of growth hormone, prolactin, and somatolactin in pituitary of the channel catfish (Ictalurus punctatus). Mar Biotechnol 3, 205-217. Taniyama, N., Kaneko, N., Inatani, Y., Miyakoshi, Y., Shimizu, M. (2016). Effects of seawater transfer and fasting on the endocrine and biochemical growth indices in juvenile chum salmon (Oncorhynchus keta). Gen Comp Endocrinol 236, 146-156. Tseng, D. Y., Chou, M. Y., Tseng, Y. C., Hsiao, C. D., Huang, C. J., Kaneko, T., Hwang, P. P. (2009). Effects of stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio) embryo. Am J Physiol Regul Integr Comp Physiol 296, R549-557. Upton, Z., Chan, S. J., Steiner, D. F., Wallace, J. C., Ballard, F. J. (1993). Evolution of insulin-like growth factor binding proteins. Growth Regu 3, 29-32. Van de Peer, Y., Mizrachi, E., Marchal, K. (2017). The evolutionary significance of polyploidy. Nat Rev Genet 18, 411-424. Wittman, M. D., Carboni, J. M., Yang, Z., Lee, F. Y., Antman, M., Attar, R., . Vyas, D. M. (2009). Discovery of a 2,4-disubstituted pyrrolo[1,2-f] [1,2,4] triazine inhibitor (BMS-754807) of insulin-like growth factor receptor (IGF-1R) kinase in clinical development. J Med Chem 52, 7360-7363. Wood, A. W., Duan, C., Bern, H. A. (2005). Insulin-like growth factor signaling in fish. Int Rev Cytol 243, 215-285. Xin, Y., Malick, A., Hu, M., Liu, C., Batah, H., Xu, H., Duan, C. (2019). Cell-autonomous regulation of epithelial cell quiescence by calcium channel Trpv6. Elife 8, e48003. Yeung, B. H., Law, A. Y., Wong, C. K. (2012). Evolution and roles of stanniocalcin. Mol Cell Endocrinol 349, 272-280. Zheng, G. D., Zhou, C. X., Lin, S. T., Chen, J., Jiang, X. Y., Zou, S. M. (2017). Two grass carp (Ctenopharyngodon idella) insulin-like growth factor-binding protein 5 genes exhibit different yet conserved functions in development and growth. Comp Biochem Physiol B Biochem Mol Biol 204, 69-76. Zhou, J., Li, W., Kamei, H., Duan, C. (2008). Duplication of the IGFBP-2 gene in teleost fish: protein structure and functionality conservation and gene expression divergence. PLoS One 3, e3926. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/8367 | - |
dc.description.abstract | 魚類鰓或胚胎表皮上的離子細胞是群負責滲透壓調節的特化細胞。目前已知胰島素 樣生長因子 (IGF) 可以調控離子運輸蛋白的表現及離子細胞型態,以適應高滲透壓的環 境。最近的研究發現,IGF 結合蛋白(IGFBP)家族中,斑馬魚 igfbp5a 在胚胎離子細胞中 對於鈣離子的恆定扮演重要的角色。大部分對於硬骨魚 IGFBP 家族的研究集中在面對 各種壓力下的生長反應,然而,很少去探究面對鹽度刺激的反應。因此,在本篇研究中, 我們利用廣鹽性魚類,印度鯖鱂魚做為調查 IGFBP 家族是否參與離子調控的模式動物。 在印度鯖鱂魚中,發現有 11 個 IGFBP 家族的基因。其中,10 型於鰓有表現(igfb1a, -1b, -2a, -2b, -3b, -4, -5a, -5b, -6a 和 -6b),但只有 igfbp5a 主要表現在鰓中。將成魚轉移到海 水後,大部分 igfbp 基因在鰓的表現量皆顯著下降。原位雜交及免疫組織化學染色的結 果顯示,igfbp5a 表現在標定離子細胞的鈉鉀離子幫浦蛋白質旁邊的較小細胞中。雙標記 原位雜交顯示出 igfbp5a 與上皮鈣離子通道(trpv6/ecac)表現位置相同,該通道是 ECaC 離子細胞的標誌,負責在淡水中吸收鈣離子。有趣的是,當胚胎適應在低鈣離子濃度環 境時,表達 igfbp5a 與 trpv6 的細胞數量顯著增加。另外,我們還發現,當阻斷 IGF 訊息 傳遞後,在低鈣環境下 trpv6 細胞數量的增加與鈣吸收的能力皆受到明顯的抑制。這些 實驗結果顯示,印度鯖鱂魚在低鈣環境時,可能通過 igfbp5a 參與 IGF 訊息傳遞的機制, 不但增加 trpv6 細胞的數量,同時也增加單細胞中鈣離子吸收的能力。 | zh_TW |
dc.description.abstract | Ionocytes in fish gills and embryonic skin are specialized cells responsible for osmoregulation. Insulin-like growth factor (IGF) signaling has been known to regulate the expression of transporters and the morphology of ionocytes for hyperosmotic adaptation. Recently, one of IGF binding protein (IGFBP), igfbp5a, has been identified in embryonic ionocytes to play a role in calcium homeostasis in zebrafish. Studies on teleost IGFBPs were focused on how they mediate growth responses to stressors, while few studies investigated the responses after salinity challenge. In the present study, we aimed to investigate whether IGFBPs participate in ion regulation in a euryhaline species, Indian medaka (Oryzias melastigma). We found totally 11 IGFBP genes in Indian medaka. Among them, ten of IGFBP members (igfb1a, -1b, -2a, -2b, -3b, -4, -5a, -5b, -6a and -6b) are expressed in the gills, but only igfbp5a is dominantly expressed. Most of branchial igfbps expression were down-regulated after seawater exposure. In situ hybridization and immunocytochemistry showed that igfbp5a was expressed in the smaller adjacent cells next to Na+-K+-ATPase-labeled ionocytes. Besides, double-labeled ISH showed that the expression of igfbp5a was co-localize with the epithelial Ca2+ channel (trpv6/ecac) which is a marker of ECaC ionocyte for Ca2+ absorption in the freshwater. Interestingly, the number of igfbp5a and trpv6 expressing cells increased when acclimated to low [Ca2+] water. In addition, blockage of IGF signaling not only inhibits the increment of trpv6 expressing cells, but also impaired the ability of Ca2+ absorption of the ionocytes after low [Ca2+] acclimation. These results suggested, through igfbp5a, IGF signaling involved in the Ca2+ absorption mechanism by increase the Ca2+ uptake and trpv6 expressing cells under low [Ca2+] water in the Indian medaka. | en |
dc.description.provenance | Made available in DSpace on 2021-05-20T00:52:53Z (GMT). No. of bitstreams: 1 U0001-2907202001200600.pdf: 4922134 bytes, checksum: 7d20eb07640582629d7e0d95015e6a2b (MD5) Previous issue date: 2020 | en |
dc.description.tableofcontents | 謝辭 i 中文摘要ii Abstract iii Contents v List of tables viii List of figures ix 1.Introduction 1 1.1 Fish ion regulation 1 1.2 Growth hormone (GH) and insulin-like growth factor 1 (IGF1) axis in osmoregulation 2 1.3 Insulin-like growth factor binding protein (IGFBP) 4 1.3.1 The IGFBP family and structural feature 4 1.3.2 IGFBP biological action 5 1.3.3 IGFBPs as regulators of ionic homeostasis 6 1.4 Indian medaka as a model to study fish ion regulation 7 1.5 Purpose 7 Materials and methods 9 2.1 Experimental animals 9 2.2 Acclimation experiments 9 2.2.1 SW transfer experiments 9 2.2.2 Low [Ca2+] artificial water transfer experiments 9 2.3 Bioinformatic analyses 10 2.4 Preparation of total RNA 10 2.5 Reverse-transcription polymerase chain reaction analysis 11 2.6 Quantitative real-time PCR 11 2.7 Whole-mount in situ hybridization 12 2.8 Immunohistochemistry 13 2.9 Measurements of whole body Ca2+ and Na+ contents 13 2.10 Scanning ion-selective electrode technique (SIET) and measurement of Ca2+ gradients 13 2.11 Pharmacological experiment 15 2.12 Statistical analysis 15 3. Results 16 3.1 Identification paralogous of igfbp gene family in database 16 3.2 Effects of salinity change on the branchial mRNA expression level of IGFBPs after transfer from FW to SW in medaka 16 3.3 Tissue distribution of IGFBPs in adult medaka in FW 16 3.4 igfbp5a expressing cells localize next to NKA expressing ionocytes in FW- acclimated medaka 17 3.5 Co-localization of igfbp5a and trpv6 in FW-acclimated medaka 17 3.6 Effects of environmental Ca2+ concentration on the expression of trpv6 and igfbp5a in medaka embryos 18 3.7 Effects of environmental Ca2+ concentration on trpv6 and igfbp5a expressing cells in medaka embryos18 3.8 Effects of blockage of the IGF signaling on the trpv6 cell number and function 18 3.9 Effects of blockage of the IGF signaling on the whole-body ion contents 19 3.10 The effects of environmental Ca2+ concentration on various type of ionocytes 19 4. Discussion 21 4.1 Potential of IGFBPs for ion regulation 21 4.2 Expression of igfbp5a in the ECaC ionocytes 23 4.3 Actions of IGFBP5a on Ca2+ homeostasis 23 4.4 The mechanism of IGFBP5a on Ca2+ homeostasis 24 4.5 Potential of IGFBP5a for Na+ regulation 26 4.6 ECaC cells presumed AC cells 27 5. Conclusion and perspectives 29 References 30 Tables and figures 41 | |
dc.language.iso | en | |
dc.title | 胰島素樣生長因子結合蛋白在鯖鱂魚離子調控之角色 | zh_TW |
dc.title | The role of insulin-like growth factor binding proteins in ion regulation of Indian medaka (Oryzias melastigma) | en |
dc.type | Thesis | |
dc.date.schoolyear | 108-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 王永松(Yung-Song Wang) | |
dc.contributor.oralexamcommittee | 周銘翊(Ming-Yi Chou),曾庸哲(Yung-Che Tseng) | |
dc.subject.keyword | 胰島素樣生長因子結合蛋白,離子調節,鈣離子恆定,離子細胞,上皮鈣離子通道,印度鯖鱂魚, | zh_TW |
dc.subject.keyword | Insulin-like growth factor binding protein,ion regulation,Ca2+ homeostasis,ionocyte,epithelial Ca2+ channel,Indian medaka, | en |
dc.relation.page | 61 | |
dc.identifier.doi | 10.6342/NTU202002011 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2020-07-30 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
dc.date.embargo-lift | 2023-07-29 | - |
顯示於系所單位: | 漁業科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
U0001-2907202001200600.pdf | 4.81 MB | Adobe PDF | 檢視/開啟 |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。