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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 朱家瑩(Chia-Ying Chu) | |
| dc.contributor.author | Chia-Yu Shih | en |
| dc.contributor.author | 施佳妤 | zh_TW |
| dc.date.accessioned | 2022-11-25T06:35:14Z | - |
| dc.date.copyright | 2021-10-13 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-10-01 | |
| dc.identifier.citation | Akao, Y., O. Marukawa, H. Morikawa, K. Nakao, M. Kamei, T. Hachiya, and Y. Tsujimoto. 1995. 'The rck/p54 candidate proto-oncogene product is a 54-kilodalton D-E-A-D box protein differentially expressed in human and mouse tissues', Cancer Research, 55: 3444-9. Anadón, C., S. Guil, L. Simó-Riudalbas, C. Moutinho, F. Setien, A. Martínez-Cardús, S. Moran, A. Villanueva, M. Calaf, A. Vidal, P. A. Lazo, I. Zondervan, S. Savola, T. Kohno, J. Yokota, L. Ribas de Pouplana, and M. Esteller. 2016. 'Gene amplification-associated overexpression of the RNA editing enzyme ADAR1 enhances human lung tumorigenesis', Oncogene, 35: 4407-13. Ayache, Jessica, Marianne Bénard, Michèle Ernoult-Lange, Nicola Minshall, Nancy Standart, Michel Kress, and Dominique Weil. 2015. 'P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes', Molecular biology of the cell, 26: 2579-95. Bass, Brenda L., and Harold Weintraub. 1988. 'An unwinding activity that covalently modifies its double-stranded RNA substrate', Cell, 55: 1089-98. Bass, Brenda L. 2002. 'RNA editing by adenosine deaminases that act on RNA', Annual review of biochemistry, 71: 817-46. Behm, M., H. Wahlstedt, A. Widmark, M. Eriksson, and M. Öhman. 2017. 'Accumulation of nuclear ADAR2 regulates adenosine-to-inosine RNA editing during neuronal development', Journal of Cell Science, 130: 745-53. Biegel, J. M., E. Henderson, E. M. Cox, G. Bonenfant, R. Netzband, S. Kahn, R. Eager, and C. T. Pager. 2017. 'Cellular DEAD-box RNA helicase DDX6 modulates interaction of miR-122 with the 5' untranslated region of hepatitis C virus RNA', Virology, 507: 231-41. Chen, J. Y., L. Stands, J. P. Staley, R. R. Jackups, Jr., L. J. Latus, and T. H. Chang. 2001. 'Specific alterations of U1-C protein or U1 small nuclear RNA can eliminate the requirement of Prp28p, an essential DEAD box splicing factor', Molecular Cell, 7: 227-32. Chen, Tian, Jian-Feng Xiang, Shanshan Zhu, Siye Chen, Qing-Fei Yin, Xiao-Ou Zhang, Jun Zhang, Hua Feng, Rui Dong, Xue-Jun Li, Li Yang, and Ling-Ling Chen. 2015. 'ADAR1 is required for differentiation and neural induction by regulating microRNA processing in a catalytically independent manner', Cell Research, 25: 459-76. Chu, Chia-ying, and Tariq M. Rana. 2006. 'Translation Repression in Human Cells by MicroRNA-Induced Gene Silencing Requires RCK/p54', PLOS Biology, 4: e210. Coller, J. M., M. Tucker, U. Sheth, M. A. Valencia-Sanchez, and R. Parker. 2001. 'The DEAD box helicase, Dhh1p, functions in mRNA decapping and interacts with both the decapping and deadenylase complexes', Rna, 7: 1717-27. Cordin, Olivier, Josette Banroques, N. Kyle Tanner, and Patrick Linder. 2006. 'The DEAD-box protein family of RNA helicases', Gene, 367: 17-37. Crow, Y. J. 1993. 'Aicardi-Goutières Syndrome.' in M. P. Adam, H. H. Ardinger, R. A. Pagon, S. E. Wallace, L. J. H. Bean, K. Stephens and A. Amemiya (eds.), GeneReviews(®) (University of Washington, Seattle Copyright © 1993-2020, University of Washington, Seattle. GeneReviews is a registered trademark of the University of Washington, Seattle. All rights reserved.: Seattle (WA)). Deffit, S. N., and H. A. Hundley. 2016. 'To edit or not to edit: regulation of ADAR editing specificity and efficiency', Wiley Interdisciplinary Reviews: RNA, 7: 113-27. Di Stefano, Bruno, En-Ching Luo, Chuck Haggerty, Stefan Aigner, Jocelyn Charlton, Justin Brumbaugh, Fei Ji, Inés Rabano Jiménez, Katie J. Clowers, Aaron J. Huebner, Kendell Clement, Inna Lipchina, Marit A. C. de Kort, Anthony Anselmo, John Pulice, Mattia F. M. Gerli, Hongcang Gu, Steven P. Gygi, Ruslan I. Sadreyev, Alexander Meissner, Gene W. Yeo, and Konrad Hochedlinger. 2019. 'The RNA Helicase DDX6 Controls Cellular Plasticity by Modulating P-Body Homeostasis', Cell Stem Cell, 25: 622-38.e13. Ekdahl, Ylva, Hossein Shahrabi Farahani, Mikaela Behm, Jens Lagergren, and Marie Öhman. 2012. 'A-to-I editing of microRNAs in the mammalian brain increases during development', Genome research, 22: 1477-87. Eulalio, Ana, Isabelle Behm-Ansmant, Daniel Schweizer, and Elisa Izaurralde. 2007. 'P-body formation is a consequence, not the cause, of RNA-mediated gene silencing', Molecular and cellular biology, 27: 3970-81. Fischer, N., and K. Weis. 2002. 'The DEAD box protein Dhh1 stimulates the decapping enzyme Dcp1', EMBO Journal, 21: 2788-97. Fritzell, Kajsa, Li-Di Xu, Jens Lagergren, and Marie Öhman. 2018. 'ADARs and editing: The role of A-to-I RNA modification in cancer progression', Seminars in Cell Developmental Biology, 79: 123-30. Fritzell, Kajsa, Li-Di Xu, Magdalena Otrocka, Claes Andréasson, and Marie Öhman. 2018. 'Sensitive ADAR editing reporter in cancer cells enables high-throughput screening of small molecule libraries', Nucleic acids research, 47: e22-e22. Gagnon, K. T., L. Li, B. A. Janowski, and D. R. Corey. 2014. 'Analysis of nuclear RNA interference in human cells by subcellular fractionation and Argonaute loading', Nature Protocols, 9: 2045-60. Gerstberger, Stefanie, Markus Hafner, and Thomas Tuschl. 2014. 'A census of human RNA-binding proteins', Nature Reviews Genetics, 15: 829-45. Haimovich, G., D. A. Medina, S. Z. Causse, M. Garber, G. Millán-Zambrano, O. Barkai, S. Chávez, J. E. Pérez-Ortín, X. Darzacq, and M. Choder. 2013. 'Gene expression is circular: factors for mRNA degradation also foster mRNA synthesis', Cell, 153: 1000-11. Han, Jian, Omer An, HuiQi Hong, Tim Hon Man Chan, Yangyang Song, Haoqing Shen, Sze Jing Tang, Jaymie Siqi Lin, Vanessa Hui En Ng, Daryl Jin Tai Tay, Fernando Bellido Molias, Priyankaa Pitcheshwar, Hui Qing Tan, Henry Yang, and Leilei Chen. 2020. 'Suppression of adenosine-to-inosine (A-to-I) RNA editome by death associated protein 3 (DAP3) promotes cancer progression', Science Advances, 6: eaba5136. Higuchi, Miyoko, Stefan Maas, Frank N. Single, Jochen Hartner, Andrei Rozov, Nail Burnashev, Dirk Feldmeyer, Rolf Sprengel, and Peter H. Seeburg. 2000. 'Point mutation in an AMPA receptor gene rescues lethality in mice deficient in the RNA-editing enzyme ADAR2', Nature, 406: 78-81. Hong, HuiQi, Omer An, Tim H. M. Chan, Vanessa H. E. Ng, Hui Si Kwok, Jaymie S. Lin, Lihua Qi, Jian Han, Daryl J. T. Tay, Sze Jing Tang, Henry Yang, Yangyang Song, Fernando Bellido Molias, Daniel G. Tenen, and Leilei Chen. 2018. 'Bidirectional regulation of adenosine-to-inosine (A-to-I) RNA editing by DEAH box helicase 9 (DHX9) in cancer', Nucleic acids research, 46: 7953-69. Huang, J. H., W. C. Ku, Y. C. Chen, Y. L. Chang, and C. Y. Chu. 2017. 'Dual mechanisms regulate the nucleocytoplasmic localization of human DDX6', Scientific Reports, 7: 42853. Kami, D., T. Kitani, A. Nakamura, N. Wakui, R. Mizutani, M. Ohue, F. Kametani, N. Akimitsu, and S. Gojo. 2018. 'The DEAD-box RNA-binding protein DDX6 regulates parental RNA decay for cellular reprogramming to pluripotency', PLoS One, 13: e0203708. Kruk, J. A., A. Dutta, J. Fu, D. S. Gilmour, and J. C. Reese. 2011. 'The multifunctional Ccr4-Not complex directly promotes transcription elongation', Genes and Development, 25: 581-93. Lehmann, K. A., and B. L. Bass. 1999. 'The importance of internal loops within RNA substrates of ADAR1', Journal of Molecular Biology, 291: 1-13. Li, Y. R., O. D. King, J. Shorter, and A. D. Gitler. 2013. 'Stress granules as crucibles of ALS pathogenesis', Journal of Cell Biology, 201: 361-72. Linder, P., and E. Jankowsky. 2011. 'From unwinding to clamping - the DEAD box RNA helicase family', Nature Reviews Molecular Cell Biology, 12: 505-16. Lunde, B. M., C. Moore, and G. Varani. 2007. 'RNA-binding proteins: modular design for efficient function', Nature Reviews Molecular Cell Biology, 8: 479-90. Matsumoto, K., O. Y. Kwon, H. Kim, and Y. Akao. 2005. 'Expression of rck/p54, a DEAD-box RNA helicase, in gametogenesis and early embryogenesis of mice', Developmental Dynamics, 233: 1149-56. Meijering, E., M. Jacob, J.-C.F. Sarria, P. Steiner, H. Hirling, and M. Unser. 2004. 'Design and validation of a tool for neurite tracing and analysis in fluorescence microscopy images', Cytometry Part A, 58A: 167-76. Miyaji, K., Y. Nakagawa, K. Matsumoto, H. Yoshida, H. Morikawa, Y. Hongou, Y. Arisaka, H. Kojima, T. Inoue, I. Hirata, K. Katsu, and Y. Akao. 2003. 'Overexpression of a DEAD box/RNA helicase protein, rck/p54, in human hepatocytes from patients with hepatitis C virus-related chronic hepatitis and its implication in hepatocellular carcinogenesis', Journal of Viral Hepatitis, 10: 241-8. Nakamura, A., R. Amikura, K. Hanyu, and S. Kobayashi. 2001. 'Me31B silences translation of oocyte-localizing RNAs through the formation of cytoplasmic RNP complex during Drosophila oogenesis', Development, 128: 3233-42. Nicklas, Sarah, Satoshi Okawa, Anna-Lena Hillje, Laura González-Cano, Antonio Del Sol, and Jens C. Schwamborn. 2015. 'The RNA helicase DDX6 regulates cell-fate specification in neural stem cells via miRNAs', Nucleic acids research, 43: 2638-54. Nishikura, K., C. Yoo, U. Kim, J. M. Murray, P. A. Estes, F. E. Cash, and S. A. Liebhaber. 1991. 'Substrate specificity of the dsRNA unwinding/modifying activity', EMBO Journal, 10: 3523-32. Ota, Hiromitsu, Masayuki Sakurai, Ravi Gupta, Louis Valente, Bjorn-Erik Wulff, Kentaro Ariyoshi, Hisashi Iizasa, Ramana V Davuluri, and Kazuko Nishikura. 2013. 'ADAR1 Forms a Complex with Dicer to Promote MicroRNA Processing and RNA-Induced Gene Silencing', Cell, 153: 575-89. Patterson, J. B., and C. E. Samuel. 1995. 'Expression and regulation by interferon of a double-stranded-RNA-specific adenosine deaminase from human cells: evidence for two forms of the deaminase', Molecular and cellular biology, 15: 5376-88. Pemberton, Kyle, Brittany Mersman, and Fenglian Xu. 2018. 'Using ImageJ to Assess Neurite Outgrowth in Mammalian Cell Cultures: Research Data Quantification Exercises in Undergraduate Neuroscience Lab', Journal of undergraduate neuroscience education : JUNE : a publication of FUN, Faculty for Undergraduate Neuroscience, 16: A186-A94. Polson, A. G., and B. L. Bass. 1994. 'Preferential selection of adenosines for modification by double-stranded RNA adenosine deaminase', EMBO journal, 13: 5701-11. Rice, G. I., P. R. Kasher, G. M. Forte, N. M. Mannion, S. M. Greenwood, M. Szynkiewicz, J. E. Dickerson, S. S. Bhaskar, M. Zampini, T. A. Briggs, E. M. Jenkinson, C. A. Bacino, R. Battini, E. Bertini, P. A. Brogan, L. A. Brueton, M. Carpanelli, C. De Laet, P. de Lonlay, M. del Toro, I. Desguerre, E. Fazzi, A. Garcia-Cazorla, A. Heiberg, M. Kawaguchi, R. Kumar, J. P. Lin, C. M. Lourenco, A. M. Male, W. Marques, Jr., C. Mignot, I. Olivieri, S. Orcesi, P. Prabhakar, M. Rasmussen, R. A. Robinson, F. Rozenberg, J. L. Schmidt, K. Steindl, T. Y. Tan, W. G. van der Merwe, A. Vanderver, G. Vassallo, E. L. Wakeling, E. Wassmer, E. Whittaker, J. H. Livingston, P. Lebon, T. Suzuki, P. J. McLaughlin, L. P. Keegan, M. A. O'Connell, S. C. Lovell, and Y. J. Crow. 2012. 'Mutations in ADAR1 cause Aicardi-Goutières syndrome associated with a type I interferon signature', Nature Genetics, 44: 1243-8. Rocak, Sanda, and Patrick Linder. 2004. 'DEAD-box proteins: the driving forces behind RNA metabolism', Nature Reviews Molecular Cell Biology, 5: 232-41. Schmitt, C., C. von Kobbe, A. Bachi, N. Panté, J. P. Rodrigues, C. Boscheron, G. Rigaut, M. Wilm, B. Séraphin, M. Carmo-Fonseca, and E. Izaurralde. 1999. 'Dbp5, a DEAD-box protein required for mRNA export, is recruited to the cytoplasmic fibrils of nuclear pore complex via a conserved interaction with CAN/Nup159p', EMBO Journal, 18: 4332-47. Smillie, D. A., and J. Sommerville. 2002. 'RNA helicase p54 (DDX6) is a shuttling protein involved in nuclear assembly of stored mRNP particles', Journal of Cell Science, 115: 395-407. Snay-Hodge, C. A., H. V. Colot, A. L. Goldstein, and C. N. Cole. 1998. 'Dbp5p/Rat8p is a yeast nuclear pore-associated DEAD-box protein essential for RNA export', EMBO Journal, 17: 2663-76. Tajirika, T., Y. Tokumaru, K. Taniguchi, N. Sugito, N. Matsuhashi, M. Futamura, K. Yanagihara, Y. Akao, and K. Yoshida. 2018. 'DEAD-Box Protein RNA-Helicase DDX6 Regulates the Expression of HER2 and FGFR2 at the Post-Transcriptional Step in Gastric Cancer Cells', International Journal of Molecular Sciences, 19. Taniguchi, K., A. Iwatsuki, N. Sugito, H. Shinohara, Y. Kuranaga, Y. Oshikawa, T. Tajirika, M. Futamura, K. Yoshida, K. Uchiyama, and Y. Akao. 2018. 'Oncogene RNA helicase DDX6 promotes the process of c-Myc expression in gastric cancer cells', Molecular Carcinogenesis, 57: 579-89. Taniguchi, K., N. Sugito, M. Kumazaki, H. Shinohara, N. Yamada, N. Matsuhashi, M. Futamura, Y. Ito, Y. Otsuki, K. Yoshida, K. Uchiyama, and Y. Akao. 2015. 'Positive feedback of DDX6/c-Myc/PTB1 regulated by miR-124 contributes to maintenance of the Warburg effect in colon cancer cells', Biochimica et Biophysia Acta, 1852: 1971-80. Tseng, S. S., P. L. Weaver, Y. Liu, M. Hitomi, A. M. Tartakoff, and T. H. Chang. 1998. 'Dbp5p, a cytosolic RNA helicase, is required for poly(A)+ RNA export', EMBO Journal, 17: 2651-62. Uddin, Mohammad Burhan, Zhishan Wang, and Chengfeng Yang. 2020. 'Dysregulations of Functional RNA Modifications in Cancer, Cancer Stemness and Cancer Therapeutics', Theranostics, 10: 3164-89. Wahlstedt, Helene, Chammiran Daniel, Mats Ensterö, and Marie Ohman. 2009. 'Large-scale mRNA sequencing determines global regulation of RNA editing during brain development', Genome research, 19: 978-86. Walkley, Carl R., and Jin Billy Li. 2017. 'Rewriting the transcriptome: adenosine-to-inosine RNA editing by ADARs', Genome biology, 18: 205-05. Wang, Y., M. Arribas-Layton, Y. Chen, J. Lykke-Andersen, and G. L. Sen. 2015. 'DDX6 Orchestrates Mammalian Progenitor Function through the mRNA Degradation and Translation Pathways', Molecular Cell, 60: 118-30. Weston, A., and J. Sommerville. 2006. 'Xp54 and related (DDX6-like) RNA helicases: roles in messenger RNP assembly, translation regulation and RNA degradation', Nucleic acids research, 34: 3082-94. Yamashita, T., M. Akamatsu, and S. Kwak. 2017. 'Altered Intracellular Milieu of ADAR2-Deficient Motor Neurons in Amyotrophic Lateral Sclerosis', Genes (Basel), 8. Yan, X., J. F. Mouillet, Q. Ou, and Y. Sadovsky. 2003. 'A novel domain within the DEAD-box protein DP103 is essential for transcriptional repression and helicase activity', Molecular and cellular biology, 23: 414-23. Yoshida-Kashikawa, M., N. Shibata, K. Takechi, and K. Agata. 2007. 'DjCBC-1, a conserved DEAD box RNA helicase of the RCK/p54/Me31B family, is a component of RNA-protein complexes in planarian stem cells and neurons', Developmental Dynamics, 236: 3436-50. Zinshteyn, Boris, and Kazuko Nishikura. 2009. 'Adenosine-to-inosine RNA editing', Wiley interdisciplinary reviews. Systems biology and medicine, 1: 202-09. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/82297 | - |
| dc.description.abstract | 當細胞分化時，基因的表現需要受到複雜且嚴密的調控，其中後轉錄調控機制過程主要由RNA結合蛋白（RNA binding proteins，RBPs）操控。DEAD-box蛋白質是RNA結合蛋白的一種，它們參與在和RNA代謝以及功能調控相關的多個面向中。此類蛋白家族中的RNA解旋酶DDX6能結合RNA誘導靜默複合體（RNA-induced silencing complex），引起RNA降解或抑制轉譯。前人的研究發現DDX6於細胞分化以及神經發育上可能扮演著關鍵的角色，然而具體調節機制仍然未知。本研究中利用免疫沈澱、蛋白質譜與西方墨點法分析，發現細胞核內的DDX6會與RNA腺苷脫氨酶(ADAR)結合。藉由本實驗室建立的雙螢光報導系統檢測細胞內ADAR 編輯RNA的活性，我們發現當細胞剔除DDX6後能使ADAR蛋白的編輯活性增加，但是ADAR蛋白質表現量卻不受影響。此外我們也發現，去除DDX6或是增加ADAR的表現皆能抑制人類神經母細胞瘤SH-SY5Y的分化。我們的結果證明了細胞核中DDX6對於調控ADAR編輯活性的新穎功能，並且提供了兩者共同影響神經細胞分化的可能機制。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-25T06:35:14Z (GMT). No. of bitstreams: 1 U0001-1309202114345000.pdf: 9122121 bytes, checksum: 51389965ec529b3b28e3f09e992f591d (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | "口試委員會審定書 i 誌謝 ii 摘要 iii Abstract iv 1. Introduction 1 1.1 RNA binding proteins and DEAD-box RNA helicases 1 1.2 DDX6 homologues and its biological significances 2 1.3 The function of DDX6 in regulating gene expression 4 1.4 DDX6 in the nucleus and its possible functions 5 1.5 A-to-I RNA editing 6 1.6 ADAR proteins in vertebrates 7 1.7 ADAR in human diseases and neuronal development 8 1.8 The regulating relationship between DDX6 and ADAR in the nucleus 9 2. Materials and methods 11 2.1 Cell culture 11 2.2 Cell transfection 11 2.3 Cloning and Constructs 11 2.4 siRNAs 12 2.5 Total cell, cytoplasmic and nuclear protein extraction 12 2.6 Immunoprecipitation 12 2.7 CRISPR/Cas9 gene knockout 13 2.8 Dual fluorescence reporter assay (DFR) 13 2.9 Western blot 14 2.10 RNA extraction, Reverse transcription and quantitative PCR (RT-qPCR) 15 2.11 Target site PCR and Sanger sequencing 15 2.12 Differentiation in neuron progenitor cells 16 2.13 MTT assay 16 2.14 Morphological analysis of differentiated cells 16 2.15 Statistical analysis 17 3. Results 18 3.1 DDX6 interacts with ADARs in the nuclei of human cells 18 3.2 Developing a dual fluorescence reporter assay monitoring A-to-I editing in cells 18 3.3 The ADAR editing activity was not significantly affected in HEK293T cells overexpressing DDX6 or treated with DDX6 siRNAs 20 3.4 DDX6 acts as a repressor on ADAR editing activity in HEK293T cells 21 3.5 The helicase activity of DDX6 is dispensable for regulating ADARs 22 3.6 The C-terminal domain of DDX6 participates in regulating ADARs 22 3.7 DDX6 can also reduce the A-to-I editing level in neuroblastoma cells 23 3.8 DDX6 is up-regulated in RA-induced differentiation of SH-SY5Y neuroblastoma cells 23 3.9 Both DDX6 and ADARs participate in neuronal differentiation 24 4. Discussion 26 4.1 DDX6 regulates the ADAR function in human cells 26 4.2 The possible factors contribute to ADAR regulation by DDX6 26 4.3 The mechanism of regulating ADARs by DDX6 28 4.4 ADAR2 expression during neuronal differentiation 29 4.5 The role of DDX6 in neuronal differentiation 30 5. References 32 6. Figures 40 7. Appendix 60 " | |
| dc.language.iso | en | |
| dc.subject | 腺苷脫氨酶 | zh_TW |
| dc.subject | 核糖核酸編輯 | zh_TW |
| dc.subject | 轉錄後修飾 | zh_TW |
| dc.subject | RNA解旋酶DDX6 | zh_TW |
| dc.subject | 細胞分化 | zh_TW |
| dc.subject | A-to-I editing | en |
| dc.subject | cell differentiation | en |
| dc.subject | ADAR | en |
| dc.subject | post-transcriptional regulation | en |
| dc.subject | RNA helicase DDX6 | en |
| dc.subject | neuroblastoma cell | en |
| dc.title | 探討RNA解旋酶DDX6在A-to-I編輯與神經分化上的角色 | zh_TW |
| dc.title | Study on the role of RNA helicase DDX6 in A-to-I editing and neuronal differentiation | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 李心予(Hsin-Tsai Liu),陳示國(Chih-Yang Tseng),周銘翊 | |
| dc.subject.keyword | RNA解旋酶DDX6,轉錄後修飾,腺苷脫氨酶,核糖核酸編輯,細胞分化, | zh_TW |
| dc.subject.keyword | RNA helicase DDX6,post-transcriptional regulation,ADAR,A-to-I editing,neuroblastoma cell,cell differentiation, | en |
| dc.relation.page | 63 | |
| dc.identifier.doi | 10.6342/NTU202103146 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2021-10-04 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生命科學系 | zh_TW |
| dc.date.embargo-lift | 2026-10-01 | - |
| 顯示於系所單位： | 生命科學系 | |
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