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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/82276完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李士傑(Shyh-Jye Lee) | |
| dc.contributor.author | Tien-Heng Han | en |
| dc.contributor.author | 韓天恒 | zh_TW |
| dc.date.accessioned | 2022-11-25T06:34:50Z | - |
| dc.date.copyright | 2021-11-09 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-10-21 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/82276 | - |
| dc.description.abstract | 發育的過程需要多種因子調節並且受到高度調控，其中微核糖核酸(microRNA)調控著轉錄後的基因靜默。過去研究顯示，miR-19a和miR-19b高度表現在心臟，而其過度表達在斑馬魚中會引起心臟發育缺陷，在大鼠新生心肌細胞誘發心臟肥大，並在心臟特異性miR-17-92轉基因小鼠中造成心臟肥大和心律不整。而其他miR-19家族成員包括miR-19d的功能及其所參與的調控仍然未知。在本論文中，我發現miR-19d可在全身包括心臟表現，而在腦表現量較高。經顯微注射miR-19d mimic以過度表現miR-19d則導致胚胎心臟缺陷，伴有心臟水腫和發育型態異常。在心臟功能分析中，miR-19d的過度表現使心臟搏動減緩、心房和心室收縮力降低以及心輸出量降低。為了研究miR-19d可能影響的基因，透過TargetScanFish的預測篩選，進一步發現過度表現miR-19d的胚胎其可能目標基因RNF122、mef2ca、has2、ucp2和myh7bb的表現量會輕微下降，表示miR-19d可能調節其轉錄或降解。透過單一RNF122 mRNA的注射發現並不能拯救過度表現miR-19d所造成的發育缺陷，其顯示miR-19d可能調控多個心臟發育相關基因。總結來說，miR-19d可能參與調節心臟早期發育相關基因及其功能，而其確切目標基因或基因群仍待探究。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-25T06:34:50Z (GMT). No. of bitstreams: 1 U0001-0410202115574400.pdf: 6750039 bytes, checksum: 4f667511d9e88ee12a0d3582df170594 (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | "口試委員會審定書 i 致謝 ii 中文摘要 iii Abstract iv Table of Contents vi List of Tables viii List of Figures ix Introduction 1 Materials and methods 7 Zebrafish maintenance and embryos collection 7 MiRNA in situ hybridization 7 Microinjection 10 Observation, imaging and parameters analyzed of heart development 10 RNA extraction, reverse transcription, and quantitative polymerase chain reaction 13 In vitro RNF122 mRNA synthesis for rescue experiments 14 Statistical analysis 15 Results 16 Phylogenetic analysis of miR-19 family genes 16 Expression pattern of zebrafish miR-19d 16 Overexpressing miR-19d affects early cardiac development 17 Overexpressing miR-19d affects RNF122, mef2ca, has2, ucp2 and myh7bb transcript levels 21 Discussion 22 References 26" | |
| dc.language.iso | en | |
| dc.subject | 發育 | zh_TW |
| dc.subject | 斑馬魚 | zh_TW |
| dc.subject | 微核糖核酸 | zh_TW |
| dc.subject | 心臟 | zh_TW |
| dc.subject | development | en |
| dc.subject | miRNA | en |
| dc.subject | heart | en |
| dc.subject | zebrafish | en |
| dc.title | 異位性表達微核糖核酸miR-19d影響斑馬魚早期心臟發育 | zh_TW |
| dc.title | Ectopic expression of miR-19d affects early heart development in zebrafish | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 蔡素宜(Hsin-Tsai Liu),周銘翊(Chih-Yang Tseng) | |
| dc.subject.keyword | 微核糖核酸,發育,心臟,斑馬魚, | zh_TW |
| dc.subject.keyword | miRNA,development,heart,zebrafish, | en |
| dc.relation.page | 43 | |
| dc.identifier.doi | 10.6342/NTU202103537 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2021-10-22 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生命科學系 | zh_TW |
| dc.date.embargo-lift | 2026-10-20 | - |
| 顯示於系所單位： | 生命科學系 | |
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