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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/82065完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 錢宗良(Chung-Liang Chien) | |
| dc.contributor.author | Shih-Ni Chang | en |
| dc.contributor.author | 張思婗 | zh_TW |
| dc.date.accessioned | 2022-11-25T05:35:04Z | - |
| dc.date.available | 2027-01-17 | |
| dc.date.copyright | 2022-01-26 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2022-01-17 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/82065 | - |
| dc.description.abstract | "血管內皮生長因子A（Vascular Endothelial Growth Factor-A, VEGF-A）是一種具有促進血管新生、神經新生及神經保護功能的生長因子。小鼠的VEGF-A具有許多同功型（isoform），在本研究中使用最常見的兩種：VEGF120與VEGF164。根據過去的文獻，VEGF120對於促進血管新生的臨床應用以及VEGF164在腦損傷後的血管新生、神經新生與神經保護方面可能十分重要。此外，有研究指出給予帶有VEGF121的腺病毒載體能改善心肌缺血模型中的側支血管發育及心肌的灌注與功能，而給予VEGF165重組蛋白則能減少中風恢復期間的神經功能缺損。因此，本研究嘗試先建立能過度表達VEGF-A的纖維母細胞株，做為未來可能之應用。 在本研究中我們將帶有組氨酸標籤（His-tag）的小鼠VEGF-A（VEGF120、VEGF164）互補核酸序列（cDNA）分別轉植入NIH/3T3纖維母細胞株。在經過藥物的篩選之後，我們建立了能過度表達VEGF-A的細胞株（3T3-neo-V120-NH、3T3-neo-V120-CH、3T3-His-V164）。接著我們利用逆轉錄聚合酶鏈式反應（RT-PCR）、即時聚合酶鏈式反應（Q-PCR）、細胞免疫染色、西方墨點法以及酶聯免疫吸附試驗（ELISA）等方法對3T3-neo-V120-NH、3T3-neo-V120-CH、3T3-His-V164細胞進行VEGF-A產物分析。由RT-PCR和Q-PCR的分析結果顯示，在3T3-neo-V120-CH細胞中，VEGF120的mRNA表現量相較於控制組有明顯地上升，而較多的VEGF-A產物也在細胞免疫染色及西方墨點法分析中被證實。在ELISA實驗結果中，我們發現3T3-neo-V120-CH細胞的VEGF-A分泌量在第1天到第3天顯著地高於控制組。而3T3-neo-V120-NH細胞與3T3-His-V164細胞的RT-PCR、細胞免疫染色和西方墨點法分析結果則顯示，我們轉植入3T3細胞株中的cDNA均未能成功表達。 由本研究的結果，我們推論3T3-neo-V120-CH細胞可以在前幾天高度表達VEGF120，而這些VEGF-A能否應用在實驗動物模式中仍需進一步的實驗來證實。" | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-25T05:35:04Z (GMT). No. of bitstreams: 1 U0001-1401202213091800.pdf: 24726398 bytes, checksum: 92101bd23859a773dd27f5caee1b9af2 (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 口試委員會審定書 i 致謝 ii 摘要 iii Abstract iv List of Figures vii List of Tables viii Abbreviation ix Chapter 1: Introduction 1 Chapter 2: Materials and Methods 5 Chapter 3: Results 15 Chapter 4: Discussion 23 Figures Figure Legends 29 Tables 53 References 56 | |
| dc.language.iso | en | |
| dc.subject | 過量表達 | zh_TW |
| dc.subject | 血管內皮生長因子 | zh_TW |
| dc.subject | 3T3纖維母細胞株 | zh_TW |
| dc.subject | 組胺酸標籤 | zh_TW |
| dc.subject | VEGF-A | en |
| dc.subject | 3T3 fibroblast | en |
| dc.subject | His-tag | en |
| dc.subject | overexpression | en |
| dc.title | 建立過量表達血管內皮生長因子之纖維母細胞株研究 | zh_TW |
| dc.title | The study of VEGFA-overexpressed NIH/3T3 fibroblast cell lines | en |
| dc.date.schoolyear | 110-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 侯珮珊(I-Hsuan Hong),廖孟琳(Kwei-Long Huang),(Jakey Blue),(Vincent F. Yu),(Jei-Zheng Wu),(Jei-Zheng Wu),(Jei-Zheng Wu) | |
| dc.subject.keyword | 血管內皮生長因子,3T3纖維母細胞株,組胺酸標籤,過量表達, | zh_TW |
| dc.subject.keyword | VEGF-A,3T3 fibroblast,His-tag,overexpression, | en |
| dc.relation.page | 62 | |
| dc.identifier.doi | 10.6342/NTU202200061 | |
| dc.rights.note | 同意授權(限校園內公開) | |
| dc.date.accepted | 2022-01-18 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 解剖學暨細胞生物學研究所 | zh_TW |
| dc.date.embargo-lift | 2027-01-17 | - |
| 顯示於系所單位： | 解剖學暨細胞生物學科所 | |
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