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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 陳示國(Shih-Kuo Chen) | |
| dc.contributor.author | Yan-Min Chiu | en |
| dc.contributor.author | 邱妍敏 | zh_TW |
| dc.date.accessioned | 2022-11-25T05:33:29Z | - |
| dc.date.available | 2026-09-01 | |
| dc.date.copyright | 2021-11-12 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-09-15 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/81977 | - |
| dc.description.abstract | "大多數的生物都具有內生性的晝夜時鐘來幫助我們有效調節生理機能，以因應一天之內的各種環境變化。許多海洋生物 ，尤其是居住在潮間帶的無脊椎動物，都被發現表現出自發性的潮汐週期行為。然而這樣的潮汐節律是由何種機制所調控的，目前還未有定論。作為生長在潮間帶的兩棲性脊椎動物，彈塗魚需適應潮汐所帶來的水位，鹽度等週期性變化，因此適合應用於潮汐週期的研究。本篇研究中紀錄並分析小彈塗魚(Periophthalmus modestus) 生活於12：12 小時光照週期及 6.5：6.5 小時水位週期下時的活動距離、活動時間，及水陸偏好隨著，並發現這三種行為皆會受到外在光照及水位刺激所調控，並表現出13及24小時的週期性。然而當水位週期被剝奪，原先較為顯著的他們潮汐週期行為則會跟隨著僅剩的日夜週期變化。而當生活於恆定低光照及恆定高水位時，行為的節律則會趨平，僅能在上陸時間上看到12小時的內生性週期。在日夜周期及水位週期適應後轉移至恆定條件中，並探查腦中的時鐘基per1b, per2, cry1a, nr1d1 ，及腦中滲透壓調節基因oxt ，鰓中離子通道基因 atp1a1 隨著時間的變化，我們發現僅有 per1b具有週期性的表現。這些結果顯示小彈塗魚隨著光照及水位改變的行為節律相對於內生性的時鐘更多受到外在刺激所調控。微弱的基因週期表現也顯示他們不容易被光照及水位調適內生性節律。這些研究發現初步地探討了兩棲性脊椎動物的內生性週期，及其可能的調控機制。 " | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-25T05:33:29Z (GMT). No. of bitstreams: 1 U0001-0809202117343300.pdf: 7591264 bytes, checksum: 3a498533e33565e486c90344bb39a205 (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | "口試委員會審訂書 i 謝 誌 iii 摘 要 v Abstract vii Contents ix Chapter I Introduction 1 1.1 Biological rhythm 1 1.1.1 Circadian rhythm 1 1.1.2 Circatidal rhythm 3 1.1.3 Circalunar rhythm 5 1.2 Model of circatidal rhythm oscillators 6 1.2.1 Circalunidian clock hypothesis 7 1.2.2 Tidal clock hypothesis 8 1.2.3 Bimodal clock hypothesis 9 1.3 Osmoregulation in teleost 10 1.3.1 Branchial ion transporters 10 1.3.2 Osmoregulatory hormones 11 1.4 Mudskippers 12 1.4.1 Information of mudskipper 12 1.4.2 Mudskipper behavior 14 1.4.3 Osmoregulation in Periophthalmus 16 Statement of purpose 19 Chapter II Material and method 21 2.1 Animals 21 2.2 Behavioral test 21 2.2.1 Entraining tank 21 2.2.2 Coordination data collection 22 2.2.3 Light and water level manipulation 23 2.3 Gene expression 24 2.3.1 Sample collection 24 2.3.2 cDNA preparation 24 2.3.3 Gene annotation 25 2.3.4 Quantitative polymerase chain reaction (qPCR) 26 2.4 Statistical analysis 26 Chapter III Results 29 3.1 Free-running behavior under constant condition after catching from field 29 3.2 Light entrainment 29 3.3 Light and water level entrainment 30 3.4 Behaviors under 12L:12D after light and water level entrainment 32 3.5 Behaviors under constant conditions after light and water level entrainment 33 3.6 Gene expression 34 Chapter IV Discussion 35 4.1 High variance in the behavioral patterns 35 4.2 Highly diverse oscillating gene expression 36 4.3 Fitting of either circatidal model 37 4.4 Differences between natural ecology and lab observation 39 4.5 Factors affecting the results 39 4.6 Circatidal rhythm and mammals 40 Significance of work 43 References 45 Appendix 101 Progrmming design for water level cycle 101 Progrmming design for data processing 104 GSB poster competition, 2021.05.28 115 The Dean Award 116" | |
| dc.language.iso | en | |
| dc.subject | 滲透壓調節 | zh_TW |
| dc.subject | 兩棲性行為 | zh_TW |
| dc.subject | 小彈塗魚 | zh_TW |
| dc.subject | 晝夜節律 | zh_TW |
| dc.subject | 潮汐節律 | zh_TW |
| dc.subject | amphibious behavior | en |
| dc.subject | circatidal rhythm | en |
| dc.subject | circadian rhythm | en |
| dc.subject | mudskipper | en |
| dc.subject | osmoregulation | en |
| dc.title | 探討彈塗魚的晝夜與潮汐節律 | zh_TW |
| dc.title | Investigation of diel and tidal rhythm in Periophthalmus modestus | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.advisor-orcid | 陳示國(0000-0002-7921-1358) | |
| dc.contributor.oralexamcommittee | 周銘翊(Hsin-Tsai Liu),黃鵬鵬(Chih-Yang Tseng),郭典翰 | |
| dc.subject.keyword | 潮汐節律,晝夜節律,小彈塗魚,滲透壓調節,兩棲性行為, | zh_TW |
| dc.subject.keyword | circatidal rhythm,circadian rhythm,mudskipper,osmoregulation,amphibious behavior, | en |
| dc.relation.page | 116 | |
| dc.identifier.doi | 10.6342/NTU202103066 | |
| dc.rights.note | 同意授權(限校園內公開) | |
| dc.date.accepted | 2021-09-16 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 基因體與系統生物學學位學程 | zh_TW |
| dc.date.embargo-lift | 2026-09-01 | - |
| Appears in Collections: | 基因體與系統生物學學位學程 | |
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|---|---|---|---|
| U0001-0809202117343300.pdf Restricted Access | 7.41 MB | Adobe PDF | View/Open |
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