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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 許正一 (Zeng-Yei Hseu) | |
| dc.contributor.author | Shi Wing Chen | en |
| dc.contributor.author | 曾思穎 | zh_TW |
| dc.date.accessioned | 2022-11-25T05:33:24Z | - |
| dc.date.available | 2021-11-02 | |
| dc.date.copyright | 2021-11-02 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-09-27 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/81972 | - |
| dc.description.abstract | "本研究在臺灣北部某一砷、銅污染場址中進行實地調查，探討污染土壤中的根圈微生物特性。採集該污染場址中的三種優勢植物，分別為毛葉腎蕨(Nephrolepis hirsutula)，台灣蘆竹(Arundo formosana Hack.)和長葉腎蕨(Nephrolepis biserrata (Sw.) Schott) ，並採集其根圈土壤與附近之非根圈土壤。採集的土壤一部分進行土壤物化性質分析（pH值，有机碳和有效性砷及銅），另一部分鮮土則用於分析微生物的群落結構特性，包括磷脂脂肪酸 (phospholipid fatty acids, PLFA) 、16S rRNA，和β-葡萄糖苷酶，酸性磷酸單酯酶和尿素酶三種酵素活性。實驗結果顯示，該場址土壤中砷和銅的全量最高分別達411 mg/kg及5404 mg/kg 。同時，根圈中的有机碳含量和EDTA萃取之有效性銅均明顯 (P < 0.05) 比非根圈土壤高，而根圈中的NaH2PO4萃取之有效砷的濃度卻降低了87%至93%，因此植物的生長增加了其根圈中的有效性銅的濃度，卻減少了有效性砷的濃度。微生物之PLFA在根圈及非根圈土壤中並沒有明顯的變化。冗餘分析結果顯示微生物PLFA與活性與土壤有效性砷及pH值呈負相關，而與有機碳呈正相關。16S rRNA的分析結果顯示，無論是非根圈或根圈土壤，其主要菌門皆為變形菌門、放線菌門、浮黴菌門、綠彎菌門、酸桿菌門及疣微菌門，佔了土壤中總菌群的90%。在微生物酵素活性方面，台灣蘆竹根圈中的β-葡萄糖苷酶、酸性磷酸單酯酶和尿素酶均顯著 (P < 0.05) 高於非根圈土壤。相較於非根圈土壤，根圈中微生物的PLFA雖然沒有顯著改變，但由於根圈土壤的有機碳增加、有效性砷減少，導致根圈中β-葡萄糖苷酶，酸性磷酸單酯酶和尿素酶的活性增加。" | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-25T05:33:24Z (GMT). No. of bitstreams: 1 U0001-1509202114342700.pdf: 3131453 bytes, checksum: 1fd340b773dbc26bfcf0d25963884239 (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 目錄 誌謝 I 摘要 II Abstract III 目錄 IV 圖目錄 VI 表目錄 VII 第一章 前言 1 第二章 文獻回顧 3 2.1 砷 3 2.2銅 3 2.3污染土壤之根圈特性 4 2.4砷與銅對土壤微生物菌落結構的影響 5 2.5砷與銅對土壤酵素活性的影響 7 2.6污染土壤中的植物對砷與銅的吸收 8 第三章 實驗步驟與方法 11 3.1 土壤與植物樣品採集 11 3.2土壤性質分析 12 3.3微生物群落結構分析 22 3.4微生物酵素活性分析 27 3.5植體分析 32 3.6統計分析 33 第四章 結果與討論 34 4.1 土壤基本理化性質 34 4.2土壤重金屬含量 35 4.3根圈與非根圈土壤的pH值 35 4.4根圈與非根圈土壤的有機碳含量 37 4.5根圈與非根圈土壤的有效性砷與銅含量 39 4.6土壤微生物種類及菌落結構 41 4.7土壤酵素活性 55 4.8 植物重金屬含量 59 4.9本研究在植生復育應用上的意義 61 第五章 結論 63 參考文獻 64 | |
| dc.language.iso | zh-TW | |
| dc.subject | 植生穩定 | zh_TW |
| dc.subject | 砷 | zh_TW |
| dc.subject | 銅 | zh_TW |
| dc.subject | 根圈 | zh_TW |
| dc.subject | 磷脂脂肪酸 | zh_TW |
| dc.subject | 16S rRNA | zh_TW |
| dc.subject | 酵素活性 | zh_TW |
| dc.subject | arsenic | en |
| dc.subject | phytostabilization | en |
| dc.subject | enzyme activity | en |
| dc.subject | 16S rRNA | en |
| dc.subject | phospholipid fatty acid (PLFA) | en |
| dc.subject | rhizosphere | en |
| dc.subject | copper | en |
| dc.title | 在砷及銅污染土壤根圈中的微生物結構及活性 | zh_TW |
| dc.title | Microbial structure and activities in rhizospheric soils contaminated with arsenic and copper | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 張義宏(Hsin-Tsai Liu),林乃君(Chih-Yang Tseng),許昺慕,廖健森 | |
| dc.subject.keyword | 砷,銅,根圈,磷脂脂肪酸,16S rRNA,酵素活性,植生穩定, | zh_TW |
| dc.subject.keyword | arsenic,copper,rhizosphere,phospholipid fatty acid (PLFA),16S rRNA,enzyme activity,phytostabilization, | en |
| dc.relation.page | 78 | |
| dc.identifier.doi | 10.6342/NTU202103191 | |
| dc.rights.note | 同意授權(限校園內公開) | |
| dc.date.accepted | 2021-09-28 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 農業化學研究所 | zh_TW |
| dc.date.embargo-lift | 2025-09-22 | - |
| 顯示於系所單位： | 農業化學系 | |
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