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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 郭典翰(Dian-Han Kuo) | |
| dc.contributor.author | Pin-Ying Chen | en |
| dc.contributor.author | 陳品縈 | zh_TW |
| dc.date.accessioned | 2022-11-25T05:33:15Z | - |
| dc.date.available | 2023-09-30 | |
| dc.date.copyright | 2021-11-06 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-09-30 | |
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Evolution of animal regeneration: re-emergence of a field. Trends Ecol Evol, 25(3), 161-170. https://doi.org/10.1016/j.tree.2009.08.005 Bely, A. E., Sikes, J. M. (2010). Acoel and platyhelminth models for stem-cell research. J Biol, 9(2), 14. https://doi.org/10.1186/jbiol223 Bely, A. E., Zattara, E. E., Sikes, J. M. (2014). Regeneration in spiralians: evolutionary patterns and developmental processes. Int J Dev Biol, 58(6-8), 623-634. https://doi.org/10.1387/ijdb.140142ab Bernfield, M., Götte, M., Park, P. W., Reizes, O., Fitzgerald, M. L., Lincecum, J., Zako, M. (1999). Functions of cell surface heparan sulfate proteoglycans. Annu Rev Biochem, 68, 729-777. https://doi.org/10.1146/annurev.biochem.68.1.729 Child, C. (1903). Studies on regulation. III. Regulative destruction of zooids and parts of zooids in Stenostoma. Archiv fur Entwickelungsmechanik der Organismen, 17, 1-40. Child, C. M. (1902). Studies on regulation. Archiv für Entwicklungsmechanik der Organismen, 15(3), 355-420. Coward, S. J. (1974). Chromatoid bodies in somatic cells of the planarian: observations on their behavior during mitosis. Anat Rec, 180(3), 533-545. https://doi.org/10.1002/ar.1091800312 De Mulder, K., Kuales, G., Pfister, D., Willems, M., Egger, B., Salvenmoser, W., Thaler, M., Gorny, A. K., Hrouda, M., Borgonie, G., Ladurner, P. (2009). Characterization of the stem cell system of the acoel Isodiametra pulchra. BMC Dev Biol, 9, 69. https://doi.org/10.1186/1471-213x-9-69 Egger, B., Steinke, D., Tarui, H., De Mulder, K., Arendt, D., Borgonie, G., Funayama, N., Gschwentner, R., Hartenstein, V., Hobmayer, B., Hooge, M., Hrouda, M., Ishida, S., Kobayashi, C., Kuales, G., Nishimura, O., Pfister, D., Rieger, R., Salvenmoser, W., Smith, J., Technau, U., Tyler, S., Agata, K., Salzburger, W., Ladurner, P. (2009). To be or not to be a flatworm: the acoel controversy. PLoS One, 4(5), e5502. https://doi.org/10.1371/journal.pone.0005502 Eisenhoffer, G. T., Kang, H., Sánchez Alvarado, A. (2008). 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E., Lin, H. (2014). PIWI proteins and PIWI-interacting RNAs function in Hydra somatic stem cells. Proc Natl Acad Sci U S A, 111(1), 337-342. https://doi.org/10.1073/pnas.1320965111 Kashima, M., Agata, K., Shibata, N. (2020). What is the role of PIWI family proteins in adult pluripotent stem cells? Insights from asexually reproducing animals, planarians. Dev Growth Differ, 62(6), 407-422. https://doi.org/10.1111/dgd.12688 Kepner, W. A., Cash, J. (1915). Ciliated pits of Stenostoma. Journal of Morphology, 26(2), 235-245. Ladurner, P., Rieger, R., Baguñà, J. (2000). Spatial distribution and differentiation potential of stem cells in hatchlings and adults in the marine platyhelminth macrostomum sp.: a bromodeoxyuridine analysis. Dev Biol, 226(2), 231-241. https://doi.org/10.1006/dbio.2000.9867 Lai, A. G., Aboobaker, A. A. (2018). EvoRegen in animals: Time to uncover deep conservation or convergence of adult stem cell evolution and regenerative processes. 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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/81965 | - |
| dc.description.abstract | 渦蟲的高度再生能力源自於其體內特有的成體幹細胞，這些幹細胞不斷進行分裂，並能分化成渦蟲體內所有的細胞型，他們也是渦蟲體內唯一能分裂增生的細胞。在所有兩側對稱生物中，只有在無腔動物亞門和扁形動物門這兩個親緣關係遙遠的類群當中有發現這種成體幹細胞。目前並不清楚這兩群動物看起來非常相似的成體幹細胞是否同源，還是獨立演化的結果。在此我將藉由研究屬於扁形動物門基群的鏈渦蟲，來檢驗幹細胞演化的假說。首先，我描述鏈渦蟲的解剖構造，並發現鏈渦蟲是具有體腔的，與其他扁蟲所呈現的無體腔狀況有顯著不同。再來，我以原位雜合反應偵測鏈渦蟲piwi、vasa等基因表現的位置。在其他動物這些基因經常表現在生殖細胞及幹細胞等處於未分化狀態的細胞。鏈渦蟲有兩個piwi基因，兩者皆表現在體壁背部中線及其附近，鏈渦蟲的四個vasa基因則表現在中胚層及腸道壁組織。另外，我使用EdU標定以及磷酸化組蛋白3的免疫染色來標記具有分裂增生活動的細胞，結果顯示在已分化的表皮、體壁中胚層、腸道壁及腎管中均有細胞分裂的活動發生，相較之下，渦蟲的piwi和vasa主要表現在體幹細胞當中，而且這些表現piwi和vasa的幹細胞也是渦蟲體內唯一能進行細胞分裂的細胞型。因此，鏈渦蟲和渦蟲的幹細胞系統並不相似。在鏈渦蟲再生的過程中，piwi1的表現範圍擴大到中胚層及腸道，這很可能代表鏈渦蟲再生的過程中可能有進行去分化。上述結果顯示鏈渦蟲體內的幹細胞系統和其他扁形動物門的物種相異，卻和外群的紐形動物門和環節動物門相似。這樣的結果比較傾向支持無腔動物門和扁形動物門的成體幹細胞系統是比較偏向獨立演化而來。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-25T05:33:15Z (GMT). No. of bitstreams: 1 U0001-2609202116060800.pdf: 90240425 bytes, checksum: dc8e61cf2961569b7fafc0406ab4f864 (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 口試委員會審定書 II 摘要 III Abstract IV Contents V Introduction 1 Biology of Stenostomum 1 Neoblast system in planarian 2 Piwi and vasa expressed in undifferentiated cells 2 Neoblast system in Acoelomorpha 4 Evolutionary origin of neoblast in Platyhelminthes and Acoelomorpha 4 Material and Methods 6 Laboratory culture of S. grande 6 Fixation and pretreatment 6 Immunofluorescence 7 Microscopic observation 8 EdU (5-ethynyl-2’-deoxyuridine) and phospho-histone 3 double labeling 9 Statistical analysis 9 Identification of piwi and vasa 10 Cloning of piwi and vasa 10 Whole mount in situ hybridization 10 Results 13 The morphology and anatomy of Stenostomum grande 13 Asexual reproduction in S. grande 15 Piwi expressed in the dorsal midline of the body wall in Stenostomum 15 Vasa expressed in the mesoderm and endoderm in Stenostomum 16 The proliferating cells distributed in body wall in S. grande 17 Regeneration of S. grande 19 The expression pattern of piwi and vasa in regeneration specimens 20 Discussion 23 The regeneration process in S. grande 23 The comparison of stem cell systems in Stenostomum and the planarians 24 The regeneration process compared between S. grande and other phylum 25 The phylogenetic position of S. grande 26 References 28 Tables and Figures 35 Supplementary figures 71 | |
| dc.language.iso | en | |
| dc.subject | 幹細胞 | zh_TW |
| dc.subject | 再生 | zh_TW |
| dc.subject | piwi | zh_TW |
| dc.subject | vasa | zh_TW |
| dc.subject | 狹口鏈渦蟲 | zh_TW |
| dc.subject | vasa | en |
| dc.subject | regeneration | en |
| dc.subject | stem cell | en |
| dc.subject | Stenostomum | en |
| dc.subject | piwi | en |
| dc.title | 狹口鏈渦蟲幹細胞系統的研究 | zh_TW |
| dc.title | Characterization of Stem Cell System in Stenostomum grande | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 朱家瑩(Hsin-Tsai Liu),陳俊宏(Chih-Yang Tseng) | |
| dc.subject.keyword | 狹口鏈渦蟲,piwi,vasa,幹細胞,再生, | zh_TW |
| dc.subject.keyword | Stenostomum,piwi,vasa,stem cell,regeneration, | en |
| dc.relation.page | 74 | |
| dc.identifier.doi | 10.6342/NTU202103368 | |
| dc.rights.note | 同意授權(限校園內公開) | |
| dc.date.accepted | 2021-10-01 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生命科學系 | zh_TW |
| dc.date.embargo-lift | 2023-09-30 | - |
| 顯示於系所單位： | 生命科學系 | |
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