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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 楊姍樺(Shan-Hua Yang) | |
| dc.contributor.author | Jia-Min Kao | en |
| dc.contributor.author | 高家敏 | zh_TW |
| dc.date.accessioned | 2022-11-25T03:06:49Z | - |
| dc.date.available | 2025-01-14 | |
| dc.date.copyright | 2021-10-21 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-09-19 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/81928 | - |
| dc.description.abstract | "珊瑚是以珊瑚共生體的形式生存，包含珊瑚宿主、共生藻以及其他共棲微生物，彼此之間形成複雜的交互作用。珊瑚白化指的是珊瑚宿主因失去共生藻或其中的光合色素而呈現骨骼的白色。除了共生藻外，其他共棲微生物亦與珊瑚的生理密不可分，其中以數量最多的共棲菌尤為關鍵。過去與熱壓力造成珊瑚白化相關的研究多是設定短期的急速升溫或是中長期的緩慢升溫和穩定高溫，本研究將兩者結合設定不同程度的日間溫度變化結合緩慢升溫進行為期一個月的水缸實驗，並以溫度累積效應（degree heating week, DHW）量化珊瑚所承受的熱壓力及其耐熱性，研究在緩慢升溫的情況下，日間溫度變化對廣布種萼形柱珊瑚（Stylophora pistillata）之生理及其共棲菌群之影響。結果顯示相較於使用平均溫度或最高溫度，以DHW來評估珊瑚是否會白化顯得更為準確。在26℃緩慢升溫至29℃並未造成熱壓力的情況下，珊瑚宿主的抗氧化酶活性及珊瑚的光系統II光化學效率維持穩定；在結合日間溫度變化±5℃造成熱壓力的情況下，珊瑚宿主的過氧化氫酶活性逐漸升高，珊瑚的光系統II光化學效率維持穩定；而在結合日間溫度變化±7℃造成更大的熱壓力的情況下，珊瑚宿主的過氧化氫酶活性逐漸升高，珊瑚的光系統II光化學效率逐漸降低至歸零，珊瑚最終白化甚至死亡，顯示珊瑚宿主已無法透過抗氧化酶有效調節活性氧物質濃度，故形成氧化傷害。另一方面，珊瑚骨骼菌群組成相對穩定，珊瑚組織菌群組成則隨著熱壓力的累積而變動，其中潛在的益生菌Acinetobacter與Pseudomonas的相對豐度隨之下降，兩者皆曾被推測可能為珊瑚抵禦病原體的第一道防線；而潛在的伺機菌Rhodobacteraceae、Alteromonadaceae、Vibrio的相對豐度則隨之上升，三者皆曾被發現在珊瑚患病或白化時大量增生。儘管相關研究曾指出大幅度日間溫度變化可能促使珊瑚適應因而增加其耐熱性、提高珊瑚面臨異常高溫的存活率；但本研究亦發現大幅度日間溫度變化可能造成熱壓力的累積、導致珊瑚白化。隨著全球氣候變遷引起的海水升溫日益嚴重，大幅度日間溫度變化帶來的熱壓力不容忽視。" | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-25T03:06:49Z (GMT). No. of bitstreams: 1 U0001-1909202112084000.pdf: 4276801 bytes, checksum: 8810bec481b3b46c9bf0132227f312af (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 謝辭 i 摘要 ii Abstract iv 目錄 vi 圖目錄 viii 表目錄 x 壹、緒論 1 （一）珊瑚與珊瑚礁生態系 1 （二）珊瑚共生體 3 （三）珊瑚共棲菌 4 （四）珊瑚白化相關研究 7 （五）熱壓力下的珊瑚生理與共棲菌群變動 9 （六）研究動機與目的 10 貳、材料與方法 12 （一）樣本採集與水缸實驗設計 12 （二）珊瑚生理分析 13 1. 熱壓力評估 13 2. 外觀記錄與光系統II光化學效率測量 14 3. 珊瑚宿主之抗氧化酶活性測定 14 4. 統計分析 15 （三）珊瑚共棲菌群分析 16 1. 去氧核醣核酸萃取 16 2. 聚合酶連鎖反應、純化與擴增子定序 17 3. 序列資料處理與統計分析 18 參、結果 20 （一）珊瑚生理分析 20 1. 水缸實驗一 20 2. 水缸實驗二 20 （二）珊瑚共棲菌群分析 21 1. 水缸實驗一 21 2. 水缸實驗二 24 肆、討論 28 （一）大幅度日間溫度變化帶來的「雙面刃效應」 28 （二）珊瑚生理之變化 30 （三）珊瑚共棲菌群之變動 32 1. 水缸實驗一 32 2. 水缸實驗二 34 伍、結論 37 陸、展望 38 柒、圖與表 39 捌、參考文獻 102 附錄 112 | |
| dc.language.iso | zh-TW | |
| dc.subject | 珊瑚共棲菌 | zh_TW |
| dc.subject | 光化學效率 | zh_TW |
| dc.subject | 抗氧化酶活性 | zh_TW |
| dc.subject | 大幅度日溫差 | zh_TW |
| dc.subject | 珊瑚白化 | zh_TW |
| dc.subject | coral bleaching | en |
| dc.subject | coral symbiotic microbiome | en |
| dc.subject | antioxidant enzyme activity | en |
| dc.subject | photochemical efficiency | en |
| dc.subject | large daily temperature fluctuations | en |
| dc.title | 日間溫度變化對萼形柱珊瑚之生理及共棲菌群之影響 | zh_TW |
| dc.title | Effects of daily temperature fluctuations on physiology and microbial composition of Stylophora pistillata | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.author-orcid | 0000-0002-1232-1085 | |
| dc.contributor.oralexamcommittee | 湯森林(Hsin-Tsai Liu),識名信也(Chih-Yang Tseng),王亮鈞,楊松穎 | |
| dc.subject.keyword | 珊瑚白化,大幅度日溫差,光化學效率,抗氧化酶活性,珊瑚共棲菌, | zh_TW |
| dc.subject.keyword | coral bleaching,large daily temperature fluctuations,photochemical efficiency,antioxidant enzyme activity,coral symbiotic microbiome, | en |
| dc.relation.page | 135 | |
| dc.identifier.doi | 10.6342/NTU202103247 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2021-09-23 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| dc.date.embargo-lift | 2025-01-14 | - |
| 顯示於系所單位： | 漁業科學研究所 | |
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