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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳穎練(Ying-Lien Chen) | |
| dc.contributor.author | Tzu-Ying Chuang | en |
| dc.contributor.author | 莊姿瑩 | zh_TW |
| dc.date.accessioned | 2022-11-25T03:06:27Z | - |
| dc.date.available | 2026-10-06 | |
| dc.date.copyright | 2021-10-15 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-10-08 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/81912 | - |
| dc.description.abstract | 馬鈴薯瘡痂病主要由馬鈴薯瘡痂病菌 (Streptomyces scabies) 造成，侵染馬鈴薯表皮組織形成凸起或凹陷之木質化壞疽病斑，破壞馬鈴薯之外觀，並進而影響其經濟價值，是田間一重要病害，台灣目前尚未有推薦之化學藥劑或微生物製劑防治此病害。本實驗室先前研究發現，液化澱粉芽孢桿菌 (Bacillus amyloliquefaciens) Ba01以及馬鈴薯晚疫病之推薦藥劑：免得爛與四氯異苯腈，對於S. scabies PS07均具有抑制效果。由於單獨施用生物防治菌於田間之效率易受到環境之影響，故本研究欲結合化學藥劑與生物製劑共同防治馬鈴薯瘡痂病，期許能提升個別防治的效率，並探討藥劑對B. amyloliquefaciens Ba01重要基因表現之影響。研究首先以濾紙片擴散試驗與搖瓶培養測試出B. amyloliquefaciens Ba01對於免得爛和四氯異苯腈耐受性較S. scabies PS07高，推測可能與B. amyloliquefaciens Ba01產生內生孢子的特性有關。利用濾紙片擴散及馬鈴薯薯片試驗，發現免得爛250、125 ppm或四氯異苯腈200、100 ppm與B. amyloliquefaciens Ba01具有共同施用之潛力，且於S. scabies PS07接種1天前、後施用處理，均有好的抑制效果。而透過盆栽試驗觀察到B. amyloliquefaciens Ba01與免得爛125 ppm (罹病度為1.1 ± 1.5%)、四氯異苯腈200 ppm (罹病度為1.1 ± 1.5%) 或100 ppm (罹病度為2.9 ± 2.0%) 稀釋下混合處理相較於只接種 S.scabies PS07之正對照組罹病度較低，然在統計上沒有顯著差異。而兩藥劑並不顯著影響B. amyloliquefaciens Ba01重要二次代謝物表面素 (surfactin) 基因 srfAD以及部分參與在氮代謝相關基因 (tnrA、glnR、codY) 的表現。後續在盆栽試驗仍要調整，使S. scabies PS07有穩定之活性與毒力及族群量，並額外測試與脫氮作用相關基因的表現，以完整藥劑對於B. amyloliquefaciens Ba01氮循環相關基因的影響。研究期望能有更完整之盆栽及田間試驗進行，以期能提供農民防治馬鈴薯瘡痂病之參考。 | zh_TW |
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| dc.description.tableofcontents | "口試委員審定書 i 致謝 ii 中文摘要 iv 英文摘要 vi 目錄 viii 表目錄 x 圖目錄 xi 1. 前言 1 1.1 馬鈴薯的介紹、常見病害及其重要性 1 1.2 馬鈴薯瘡痂病 2 1.3 現行馬鈴薯瘡痂病之防治方法 3 1.4 液化澱粉芽孢桿菌 5 1.5 化學藥劑結合生物防治菌Bacillus species之相關研究 6 1.6 實驗室前人研究及研究目的 7 2. 材料與方法 9 2.1 供試農藥成品及培養基 9 2.2 試驗細菌菌株與培養條件 9 2.3 測試免得爛與四氯異苯腈對於S. scabies PS07及B. amyloliquefaciens Ba01之影響 10 2.3.1 濾紙片擴散試驗 (Disk diffusion test) 10 2.3.2 搖瓶培養 (Shake flask assay) 10 2.4 探討免得爛與四氯異苯腈分別與B. amyloliquefaciens Ba01共同施用之合適濃度與施用方法 11 2.4.1 濾紙片擴散試驗 11 2.4.2 馬鈴薯薯片試驗 (Potato tuber slice assay) 12 2.4.3 盆栽試驗 (Pot assay) 13 2.5 藥劑對於B. amyloliquefaciens Ba01表面素 (surfactin) 及氮代謝生合成相關基因表現量之影響 14 2.5.1 B. amyloliquefaciens Ba01 RNA純化 14 2.5.2 cDNA合成 15 2.5.3 即時反轉錄聚合酶連鎖反應 (Quantitative reverse transcription PCR, RT-qPCR) 15 3. 結果 17 3.1 免得爛與四氯異苯腈對於B. amyloliquefaciens Ba01之影響小於S. scabies PS07 17 3.2 免得爛250 ppm或四氯異苯腈200 ppm與B. amyloliquefaciens Ba01混合對於S. scabies PS07均具有較好的抑制效果 18 3.3 於接種S. scabies PS07一天前或後施用免得爛250、125 ppm或四氯異苯腈200、100 ppm與B. amyloliquefaciens Ba01混合處理對於S. scabies PS07均有好的抑制效果 18 3.4 免得爛125 ppm或四氯異苯腈200及100 ppm與B. amyloliquefaciens Ba01混合施用能降低馬鈴薯瘡痂病罹病度 19 3.5 免得爛與四氯異苯腈不影響B. amyloliquefaciens Ba01在表面素生成及部分氮代謝基因的表現 19 4. 討論 21 5. 參考文獻 26 6. 圖表 35" | |
| dc.language.iso | zh-TW | |
| dc.subject | 表面素 | zh_TW |
| dc.subject | 免得爛 | zh_TW |
| dc.subject | 馬鈴薯瘡痂病 | zh_TW |
| dc.subject | 液化澱粉芽孢桿菌 | zh_TW |
| dc.subject | 四氯異苯腈 | zh_TW |
| dc.subject | 氮代謝 | zh_TW |
| dc.subject | Bacillus amyloliquefaciens | en |
| dc.subject | nitrogen metabolism | en |
| dc.subject | surfactin | en |
| dc.subject | chlorothalonil | en |
| dc.subject | metiram | en |
| dc.subject | Potato common scab | en |
| dc.title | 防治馬鈴薯瘡痂病之化學藥劑及生物防治菌之應用 | zh_TW |
| dc.title | Application of chemical and biocontrol agents in managing potato common scab | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 曾敏南(Hsin-Tsai Liu),蔡佳欣(Chih-Yang Tseng) | |
| dc.subject.keyword | 馬鈴薯瘡痂病,免得爛,四氯異苯腈,液化澱粉芽孢桿菌,表面素,氮代謝, | zh_TW |
| dc.subject.keyword | Potato common scab,metiram,chlorothalonil,Bacillus amyloliquefaciens,surfactin,nitrogen metabolism, | en |
| dc.relation.page | 50 | |
| dc.identifier.doi | 10.6342/NTU202103590 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2021-10-08 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 植物病理與微生物學研究所 | zh_TW |
| dc.date.embargo-lift | 2026-10-06 | - |
| 顯示於系所單位： | 植物病理與微生物學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| U0001-0610202117551200.pdf 此日期後於網路公開 2026-10-06 | 2.89 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。