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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/81853完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 游佳欣(Jiashing Yu) | |
| dc.contributor.author | Fang-Jung Chen | en |
| dc.contributor.author | 陳方榕 | zh_TW |
| dc.date.accessioned | 2022-11-25T03:05:06Z | - |
| dc.date.available | 2026-07-19 | |
| dc.date.copyright | 2021-08-18 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-07-20 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/81853 | - |
| dc.description.abstract | " 導電高分子在生醫領域的應用隨著幹細胞療程及生物訊號探測的發展也越來越受到注目。首先，由導電高分子所製備而成的可導電生醫材料與電刺激的結合可以促進神經、硬骨以及肌肉的組織再生，使其在組織工程領域中發展成為相當有潛力的材料。許多研究已致力於導電高分子所製成的二維材料在分化上的應用，但三維孔洞結構的應用還未被多加關注。另外，導電水凝膠的發展在生物感測器的需求提升之下也越來越受到注目。然而在傳統上合成導電水凝膠時往往會混參含有細胞毒性的化合物或金屬。 因此，在這份研究中導電高分子聚(3,4-乙烯基二氧噻吩):聚(苯乙烯磺酸鹽) (PEDOT:PSS) 首先結合了奈米碳管 (MWCNT) 用於製備三維導電支架。電刺激結合PEDOT:PSS及MWCNT所作成的三維支架被應用於人類脂肪幹細胞的硬骨分化。研究結果顯示人類脂肪幹細胞可以成功地在支架內部生長，代表此導電三維支架的低細胞毒性。更重要的是，硬骨分化的分析結果顯示鈣沉積及硬骨分化特定基因在電刺激的作用下都可以顯著地提高。綜合這些結果表示PEDOT:PSS及MWCNT所製成的三維高孔洞支架可作為電刺激應用於幹細胞硬骨分化的平台。 另一方面，PEDOT:PSS更被混合入可光交聯的甲基丙烯酸酐化明膠 (GelMA) 來合成可導電的水凝膠。PEDOT:PSS及GelMA混合而成的水凝膠更進一步使用鈣離子進行物理性交聯，而結果顯示額外使用鈣離子交聯的導電水凝膠具有較低的彭潤效果及較慢的降解速率，使得PEDOT:PSS及GelMA所製備而成的水凝膠擁有可調控的物理及化學性質。而在細胞實驗中，結果也顯示此導電水凝膠具有低細胞毒性及良好的生物相容性，且可使小鼠成纖維母細胞L929在水凝膠內部增殖且生長。後續仍須進行更多實驗以及文獻來佐證導電水凝膠在電刺激組織工程或是生物訊號探測應用是否確有其效果。" | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-25T03:05:06Z (GMT). No. of bitstreams: 1 U0001-0607202113060600.pdf: 18472936 bytes, checksum: 8a9a026d5f9acdac880a9fb41570741c (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 致謝 i 摘要 iv Abstract vi Contents viii List of Figures xiii List of Tables xvii List of Equations xviii Chapter 1 Introduction 1 1.1 Tissue Engineering 1 1.1.1 Tissue engineering introduction 1 1.1.2 Bone tissue engineering 2 1.1.3 Electrical stimulation on bone tissue engineering 3 1.2 Conductive polymers 3 1.2.1 Conductive polymer introduction 3 1.2.2 PEDOT:PSS 4 1.3 Three-dimensional scaffold 6 1.3.1 3D scaffold introduction 6 1.3.2 Fabrication methods for PEDOT:PSS porous scaffold 6 1.3.3 PEDOT:PSS scaffold incorporation with carbon nanotube 7 1.4 Hydrogel 8 1.4.1 Hydrogel introduction 8 1.4.2 Photo-crosslinkable hydrogel 9 1.4.3 Conductive hydrogel 10 1.4.4 GelMA hydrogel incorporation with PEDOT:PSS 11 1.5 Motivation and Aims 11 1.6 Research Framework 14 Chapter 2 Materials and Methods 15 2.1 Materials 15 2.1.1 Fabrication of PEDOT:PSS/MWCNT scaffold 15 2.1.2 Fabrication of PEDOT:PSS/GelMA hydrogel 15 2.1.3 Degradation test 15 2.1.4 Cell culture 16 2.1.5 Cell viability and proliferation 17 2.1.6 Cell differentiation 17 2.1.7 Calcium quantification 17 2.1.8 Immunofluorescence staining 17 2.1.9 Real-time quantitative polymerase chain reaction (qPCR) 18 2.2 Equipment 19 2.3 Solution formula 21 2.4 Methods 24 2.4.1 Fabrication of PEDOT:PSS/MWCNT scaffold 24 2.4.2 Fabrication of PEDOT:PSS/GelMA hydrogel 25 2.4.3 Dynamic compression test 26 2.4.4 Swelling test 26 2.4.5 Conductivity test 26 2.4.6 in vitro degradation test 27 2.4.7 3D bio-printing 28 2.4.8 Cell culture 28 2.4.9 Cytotoxicity test 29 2.4.10 Osteogenic differentiation in PEDOT:PSS/MWCNT scaffold 31 2.4.11 Characteristics of osteogenic differentiated hASC 33 2.4.12 Electrical stimulation 34 2.4.13 RNA extraction 37 2.4.14 Reverse transcription of RNA 37 2.4.15 Real-time polymerase chain reaction (qPCR) 38 2.4.16 Statistical analysis 38 Chapter 3 Results and discussion 39 3.1 Characterizations of PEDOT:PSS/MWCNT scaffold 39 3.1.1 Physical properties of PEDOT:PSS/MWCNT scaffold 39 3.1.2 Dynamic compression of PEDOT:PSS/MWCNT scaffold 42 3.1.3 Discussion 43 3.2 Biocompatibility of PEDOT:PSS/MWCNT scaffold 48 3.2.1 Cell activity 48 3.2.2 Cell viability 49 3.2.3 Cell adhesion 50 3.2.4 Discussion 51 3.3 hASC osteogenesis potential in PEDOT:PSS/MWCNT scaffold 56 3.3.1 Cell morphology 56 3.3.2 Calcium deposition quantification 57 3.3.3 Discussion 58 3.4 hASC osteogenesis performance with electrical stimulation (ES) 61 3.4.1 Electrical stimulation parameter 61 3.4.2 Cell morphology 63 3.4.3 Calcium deposition quantification 64 3.4.4 Immunocytochemistry fluorescence staining 66 3.4.5 qPCR 67 3.4.6 Discussion 68 3.5 Characterizations of PEDOT:PSS/GelMA hydrogel 76 3.5.1 Appearance and microstructure of hydrogel 76 3.5.2 Swelling behavior 78 3.5.3 Enzymatic degradation 79 3.5.4 Long-term degradation 80 3.5.5 Three-dimensional bioprinting of PEDOT:PSS/GelMA hydrogel 81 3.5.6 Discussion 83 3.6 Biocompatibility of PEDOT:PSS/GelMA hydrogel 91 3.6.1 Cytotoxicity of PEDOT:PSS/GelMA hydrogel 91 3.6.2 Proliferation of encapsulated L929 in PEDOT:PSS/GelMA hydrogel 92 3.6.3 Cell morphology of L929 contacted with PEDOT:PSS/GelMA hydrogel 93 3.6.4 Discussion 94 Conclusion 98 References 100 | |
| dc.language.iso | en | |
| dc.subject | 硬骨組織再生 | zh_TW |
| dc.subject | 孔洞支架 | zh_TW |
| dc.subject | 導電高分子 | zh_TW |
| dc.subject | 電刺激 | zh_TW |
| dc.subject | 幹細胞 | zh_TW |
| dc.subject | 導電水凝膠 | zh_TW |
| dc.subject | electrical stimulation | en |
| dc.subject | conductive hydrogel | en |
| dc.subject | bone tissue regeneration | en |
| dc.subject | conductive polymer | en |
| dc.subject | PEDOT:PSS | en |
| dc.subject | porous scaffold | en |
| dc.subject | stem cell | en |
| dc.title | 以導電高分子製備之三維孔洞支架及水凝膠在組織再生工程上的應用 | zh_TW |
| dc.title | Fabrication of Three-dimensional Porous Scaffold and Hydrogel from Conductive Polymer for Tissue Regeneration | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 羅世強(Hsin-Tsai Liu),蕭育生(Chih-Yang Tseng),陳賢燁 | |
| dc.subject.keyword | 導電高分子,孔洞支架,電刺激,幹細胞,硬骨組織再生,導電水凝膠, | zh_TW |
| dc.subject.keyword | conductive polymer,PEDOT:PSS,porous scaffold,electrical stimulation,stem cell,bone tissue regeneration,conductive hydrogel, | en |
| dc.relation.page | 107 | |
| dc.identifier.doi | 10.6342/NTU202101295 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2021-07-20 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
| dc.date.embargo-lift | 2026-07-19 | - |
| 顯示於系所單位： | 化學工程學系 | |
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| U0001-0607202113060600.pdf 此日期後於網路公開 2026-07-19 | 18.04 MB | Adobe PDF |
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