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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 徐善慧(Shan-hui Hsu) | |
| dc.contributor.author | Ting-Chieh Chen | en |
| dc.contributor.author | 陳廷杰 | zh_TW |
| dc.date.accessioned | 2022-11-24T09:24:38Z | - |
| dc.date.available | 2022-11-24T09:24:38Z | - |
| dc.date.copyright | 2022-02-21 | |
| dc.date.issued | 2022 | |
| dc.date.submitted | 2022-02-10 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/81602 | - |
| dc.description.abstract | 冷凍凝膠的優點是具有高度多孔性，並具有足夠的機械穩定性和可注射性，可用於生物醫學應用。當前，三維（3D）列印被用於生產組織工程的客製化支架。但是到目前為止，關於冷凍凝膠的3D列印研究很少，因為冷凍凝膠的熱傳因素導致其應用上的限制。我們之前的研究中，合成了各種雙官能聚胺酯（DPU）奈米粒子交聯劑，它們與殼聚醣（CS）在4°C下反應4小時，作為3D列印的進料（預冷凍凝膠）。將列印後的預冷凍凝膠在-20°C下冷凍形成3D列印的冷凍凝膠。三維列印冷凍凝膠具有與塊狀冷凍凝膠相似的特性，例如：形狀恢復特性和吸水率（≈3200％）。細胞培養實驗的結果表明3D列印的冷凍凝膠支架可促進人間充質乾細胞的增殖和軟骨分化，並提供出色的機械完整性。具有可注射性和形狀恢復特性的3D列印冷凍凝膠支架在組織工程和微創手術中擁有應用潛力。 | zh_TW |
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| dc.description.tableofcontents | 目錄 致謝 I 摘要 II Abstract III 目錄 IV 圖目錄 VIII 表目錄 X 第一章 文獻回顧 1 1.1. 冷凍凝膠的發展與應用 1 1.2. 殼聚醣的生醫應用與限制 1 1.3. 3D列印技術的製備方式 2 1.4. 水性生物可降解聚胺酯交聯劑的生醫應用 2 1.5. 研究目的 4 第二章 研究方法 5 2.1. 研究架構 5 2.2. DL型聚乳酸二元醇之合成與鑑定 6 2.2.1. DL型聚乳酸二元醇之合成 6 2.2.2. DL型聚乳酸二元醇之分子量鑑定 7 2.3. 雙官能基聚胺酯交聯劑之材料製備與分析 8 2.3.1.雙官能基聚胺酯交聯劑之合成 8 2.3.2. 雙官能基聚胺酯交聯劑膜之製備 10 2.3.3. 膠體滲透層析儀 10 2.3.4. 奈米粒徑和表面電位分析儀 10 2.3.5. 衰減式全反射（ATR-FTIR）傅立葉紅外光譜分析 11 2.3.6. 化學分析影像能譜儀分析 11 2.3.7. 廣角度X光繞射儀 12 2.4. 不同種類的交聯劑之冷凍凝膠的製備 12 2.4.1. 冷凍凝膠之吸水能力測試 12 2.4.2. 冷凍凝膠之動態力學測試 13 2.4.3. 冷凍凝膠模擬生理環境降解測試 13 2.4.4. 冷凍凝膠含細胞培養降解實驗 13 2.5. 不同時間的雙官能基聚胺酯交聯劑之預冷凍凝膠優化 14 2.5.1 原位小角度X射線散射分析儀 14 2.5.2. 流變性質檢測（Rheology） 15 2.6. 預冷凍凝膠3D列印 15 2.7. 3D列印冷凍凝膠的物理化學性質分析 16 2.7.1. 冷凍凝膠及3D列印冷凍凝膠之孔隙度與孔徑比較 16 2.7.2. 冷凍凝膠及3D列印冷凍凝膠之吸水率測試 17 2.7.3. 冷凍凝膠及3D列印冷凍凝膠之動態力學分析 17 2.7.4. 冷凍凝膠之體外降解測試 17 2.8. 3D列印冷凍凝膠可注射能力測試 17 2.8.1. 傳統冷凍凝膠之可注射能力測試 17 2.8.2. 3D列印冷凍凝膠之可注射能力測試 18 2.9. 人脂肪幹細胞（hADSCs）相容性測試 18 2.9.1. hADSCs增殖測試 18 2.9.2. hADSCs螢光染色測試 19 2.9.3. hADSCs縮時攝影測試 19 2.9.4. hADSCs分化之基因表現測試 19 2.9.5. hADSCs分化之醣胺聚醣（GAG）分析 20 2.9.6. hADSCs分化之safranin O/fast green染色分析 20 2.10. 統計分析 21 第三章 實驗結果 22 3.1. 雙官能基聚胺酯交聯劑性質之基本分析 22 3.1.1. DL型聚乳酸二元醇之核磁共振分析 22 3.1.2. 分子量、粒徑、電負度測試 22 3.1.3. X射線光電子能譜分析 23 3.1.4. 傅立葉紅外光譜儀 23 3.1.5 化學分析影像能譜儀 23 3.2. 殼聚醣與聚胺酯交聯形成冷凍凝膠 24 3.2.1. 不同組成比例的冷凍凝膠吸水程度測試 24 3.2.2. 水合冷凍凝膠之機械性質測試 25 3.3. 3D列印之預冷凍凝膠墨水最佳化 26 3.3.1. 巨觀預冷凍凝膠的注射性測試 26 3.3.2.小角度X射線散射測試 27 3.3.3. 預冷凍凝膠之流變學測試 28 3.4. 3D列印之冷凍凝膠 29 3.4.1. 3D列印冷凍凝膠的列印製備過程 29 3.4.2. 3D列印冷凍凝膠巨觀測試 30 3.5. 3D列印冷凍凝膠製程與傳統冷凍凝膠製程的比較 30 3.5.1. 傳統冷凍凝膠的製備與預冷凍凝膠的製備 30 3.5.2. 穿透式電子顯微鏡分析 31 3.5.3. 不同冷凍凝膠之機械性質分析 31 3.5.4. 不同冷凍凝膠之注射實驗測試 32 3.6. 3D 列印冷凍凝膠之生物相容性測試 32 3.6.1. hADSCs細胞型態變化 32 3.6.2. hADSCs 增殖程度測試 33 3.6.3. hADSCs分化之基因表現測試 33 3.6.4. hADSCs分化之醣胺聚醣（GAG）分析與染色分析 34 第四章 討論 35 4.1. 可列印冷凍凝膠之限制與發展 35 4.2. 克服限制並製備3D列印冷凍凝膠的優勢 35 4.3. 不同CSDPU冷凍凝膠在微觀與機械性質的討論 36 4.4. 製備3D列印冷凍凝膠的條件與優勢 37 4.5. 3D列印冷凍凝膠與傳統冷凍凝膠的性質比較 39 4.6. 3D列印冷凍凝膠的生物相容性 40 4.7. 3D列印冷凍凝膠的潛在利用 41 第五章 結論 42 參考文獻 43 | |
| dc.language.iso | zh-TW | |
| dc.subject | 可注射性 | zh_TW |
| dc.subject | 殼聚醣 | zh_TW |
| dc.subject | 3D列印 | zh_TW |
| dc.subject | 聚胺酯 | zh_TW |
| dc.subject | 冷凍凝膠 | zh_TW |
| dc.subject | cryogel | en |
| dc.subject | injectability | en |
| dc.subject | Chitosan | en |
| dc.subject | 3D-printing | en |
| dc.subject | polyurethane | en |
| dc.title | 具有注射特性和形狀恢復特性的三維列印冷凍凝膠支架 | zh_TW |
| dc.title | Three-Dimensional Printing of Cryogel as Injectable and Shape Recoverable Scaffolds | en |
| dc.date.schoolyear | 110-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 賴育英(Tzi-Dar Chiueh),侯詠德(Hsin-Shu Chen) | |
| dc.subject.keyword | 殼聚醣,3D列印,聚胺酯,冷凍凝膠,可注射性, | zh_TW |
| dc.subject.keyword | Chitosan,3D-printing,polyurethane,cryogel,injectability, | en |
| dc.relation.page | 72 | |
| dc.identifier.doi | 10.6342/NTU202200506 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2022-02-11 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 高分子科學與工程學研究所 | zh_TW |
| 顯示於系所單位： | 高分子科學與工程學研究所 | |
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