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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李昆達(Kung-Ta Lee) | |
| dc.contributor.author | Yen-Ning Chang | en |
| dc.contributor.author | 張晏寧 | zh_TW |
| dc.date.accessioned | 2022-11-24T03:14:34Z | - |
| dc.date.available | 2021-11-05 | |
| dc.date.available | 2022-11-24T03:14:34Z | - |
| dc.date.copyright | 2021-11-05 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-10-15 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/80733 | - |
| dc.description.abstract | 植物在面臨危險諸如環境壓力、受損及食草動物攻擊時，植物會生產二級代謝物。當Nicotiana tabacum受到損傷或是食草動物攻擊時，會釋出茄科植物特有的信號胜肽──系統素並從受傷細胞傳遞至整株植株，誘導茉莉酸信號途徑進而刺激下游防禦途徑以生產尼古丁；而外添加茉莉酸甲酯刺激茉莉酸信號途徑同樣會增加菸草的尼古丁累積。為了想進一步提升菸草毛狀根中的尼古丁累積量，實驗室先前研究嘗試對菸草毛狀根同時添加系統素及茉莉酸甲酯，然而，在實驗結果卻發現，比起只添加茉莉酸甲酯，混合添加茉莉酸甲酯及系統素含有較低的尼古丁累積量，額外的系統素添加並未增加更多尼古丁累積反而抑制來自茉莉酸甲酯的刺激。為了更深入了解此一現象，首先進行了不同菸草組織的確認，以不同株菸草毛狀根及菸草幼苗實驗，結果顯示在尼古丁含量上有類似模式，確認此現象可能存在於不同菸草組織。為了解其中抑制及調節機制，選取有關系統素誘導、尼古丁合成及傳送、多個植物激素誘導基因等進行基因表現分析。根據基因表現量分析結果，混合添加組比起單添加茉莉酸甲酯組在茉莉酸標記基因及尼古丁生合成基因表現量上調，其基因表現結果與代謝物結果相反，且乙烯標記基因表現量也有上調的情形。因此，我們提出假說，額外添加系統素於茉莉酸甲酯的背景下，系統素可能對茉莉酸信號途徑及乙烯信號途徑產生影響，且造成影響位於核酸層級以及代謝物層級前，導致了尼古丁累積量的下降。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-24T03:14:34Z (GMT). No. of bitstreams: 1 U0001-1510202102525600.pdf: 1801835 bytes, checksum: a77e01ed800b43d4c59c0a29afba6013 (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 誌謝 i Abstract ii 中文摘要 iv Content v List of Tables and Figures vii Chapter 1: Introduction 1 1.1. Classification and production of plant secondary metabolites 1 1.2. Hairy root cultures 3 1.3. Plant defense response and jasmonate 4 1.4. Systemin in Solanaceae 5 1.5. Nicotine biosynthesis and transportation in Nicotiana tabacum 6 Chapter 2: Materials and methods 8 2.1. Plant materials 8 2.1.1. Hairy root culture 8 2.1.2. Seedling germination 8 2.2. Peptide and plant hormone application 9 2.2.1. Peptide and plant hormone preparation 9 2.2.2. Systemin and plant hormone treatment 9 2.3. Nicotine quantification 10 2.3.1. Nicotine extraction 10 2.3.2. UPLC condition 10 2.4. Gene expression analysis 11 2.4.1. RNA extraction and purification 11 2.4.2. First strand cDNA synthesis 12 2.4.3. Reverse transcription quantitative real-time PCR (RT-qPCR) 12 Chapter 3: Results 14 3.1. Effect of MeJA/TomSys treatment of methyl jasmonate and TomSys in tobacco hairy root clone 32 and 230. 14 3.2. Comparing effect of dosage of MeJA and TomSys between different clone of tobacco hairy roots. 15 3.3. Method development in tobacco seedlings 16 3.4. Effect of mixed treatment of methyl jasmonate and systemin on metabolites in tobacco seedlings. 18 3.5. Effect of different dosage of TomSys single treatment on metabolite in tobacco seedlings. 18 3.6. Effect of mixed treatment in JA-induced nicotine biosynthesis related gene of gene expression 19 3.7. Effect of mixed treatment in other hormone of gene expression 21 Chapter 4: Discussion 23 4.1. Treatment of MeJA and TomSys in different clone or tissue. 23 4.2. TomSys treatment induced response in tobacco. 25 4.3. Comparison between metabolites and gene expression results. 26 4.4. Marker gene of jasmonate-signaling pathway did not up-regulate after treating exogenous MeJA in seedlings. 27 Chapter 5: Conclusion 29 Abbreviation 30 References 42 | |
| dc.language.iso | en | |
| dc.subject | 二級代謝物 | zh_TW |
| dc.subject | 尼古丁 | zh_TW |
| dc.subject | 系統素 | zh_TW |
| dc.subject | 茉莉酸甲酯 | zh_TW |
| dc.subject | 毛狀根 | zh_TW |
| dc.subject | 菸草 | zh_TW |
| dc.subject | systemin | en |
| dc.subject | secondary metabolites | en |
| dc.subject | methyl jasmonate | en |
| dc.subject | hairy roots | en |
| dc.subject | nicotine | en |
| dc.subject | Nicotiana tabacum | en |
| dc.title | Systemin 對茉莉酸甲酯誘導菸草毛狀根及幼苗中的尼古丁累積之影響 | zh_TW |
| dc.title | The Effect of Systemin on the Methyl Jasmonate-induced Nicotine Accumulation in Tobacco Hairy Roots and Seedlings | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 楊健志(Hsin-Tsai Liu),劉啟德(Chih-Yang Tseng),賴爾珉,陳佩燁 | |
| dc.subject.keyword | 系統素,毛狀根,茉莉酸甲酯,菸草,尼古丁,二級代謝物, | zh_TW |
| dc.subject.keyword | systemin,hairy roots,methyl jasmonate,Nicotiana tabacum,nicotine,secondary metabolites, | en |
| dc.relation.page | 46 | |
| dc.identifier.doi | 10.6342/NTU202103745 | |
| dc.rights.note | 同意授權(限校園內公開) | |
| dc.date.accepted | 2021-10-15 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| 顯示於系所單位： | 生化科技學系 | |
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