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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林晉玄(Ching-Hsuan Lin) | |
| dc.contributor.author | Chih-Chieh Hsu | en |
| dc.contributor.author | 許祉婕 | zh_TW |
| dc.date.accessioned | 2022-11-24T03:07:44Z | - |
| dc.date.available | 2022-02-16 | |
| dc.date.available | 2022-11-24T03:07:44Z | - |
| dc.date.copyright | 2022-02-16 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2022-02-09 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/80488 | - |
| dc.description.abstract | "近年來台灣醫院院內真菌感染的比例逐年上升，其中由白色念珠菌(Candida albicans)所造成的感染佔台灣院內真菌感染比例的一半以上，原因除了罹患後天免疫不全症候群的患者增加，以及因器官移植而使用免疫抑制劑與接受癌症化療等治療導致免疫力低落的病患增加，都使得原先為人體共生菌的白色念珠菌伺機感染人體。 先前實驗室發現到白色念珠菌中ZCF31基因剔除後(Yu-Ting Liao,尚未發表)會導致白色念珠菌zcf31∆突變株的細胞壁增厚，ZCF31 (ORF19.5924)基因為zinc cluster transcription factor (ZCF)之一，在ZCF蛋白質家族中約有80種蛋白質，而目前已知ZCF15與ZCF29和真菌對抗環境中ROS有關，同時也發現ZCF32可以負調控生物膜(biofilm)的形成，但針對於ZCF31的功能還尚未明瞭。 為了瞭解ZCF31基因在白色念珠菌中所扮演的功能，本研究將分為兩個部份做探討，第一部分是檢驗白色念珠菌的致病以及型態轉換能力，了解ZCF31基因的剔除對於念珠菌所產生的影響，針對與白色念珠菌致病力相關的指標：例如附著力(adhesion)、生物膜的生成(biofilm formation)以及opaque-white 轉換等能力進行分析。第二部分則是利用Macrophage Phagocytosis Assay檢驗野生型白色念珠菌SC5314及zcf31∆突變株與巨噬細胞共培養後，巨噬細胞的吞噬情形是否會有差異，進而了解ZCF31的缺失對於白色念珠菌在面對宿主免疫系統會有何影響，最後也透過動物實驗，檢驗在生物體的環境下ZCF31的剔除對於小鼠的存活率所產生的影響。 目前研究結果雖然無法證明基因zcf31刪除後在致病能力上有所改變，但動物實驗顯現出zcf31∆突變株在感染前期似乎具有較高的死亡率，而感染後期zcf31∆突變株的存活率最高，未來希望可以釐清在白色念珠菌中ZCF31基因在致病能力中的功能，同時也了解ZCF31基因剔除後細胞壁增厚的現象對於免疫反應的影響。 " | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-24T03:07:44Z (GMT). No. of bitstreams: 1 U0001-1409202114480700.pdf: 4845788 bytes, checksum: ac19050f909d49ee93dd6501ee4f7550 (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 口試委員會審定書 I 誌謝 II 中文摘要 III Abstract V 目錄 VI 表目錄 IX 圖目錄 X 第一章 文獻回顧 1 第二節 菌絲生長(Hyphae formation) 2 第三節 White-opaque型態轉換 3 第四節 念珠菌貼附能力(Adhesion) 4 第五節 生物膜生成(Biofilm formation) 5 第六節 菌絲入侵能力(Invasion) 6 第七節 ZCF31基因 7 第八節 吞噬作用 8 第二章 實驗目的 8 第三章 材料方法 10 第一節 實驗材料 10 第二節 實驗方法 10 白色念珠菌培養 10 細胞培養 10 白色念珠菌野生型SC5314與zcf31∆突變株white-opaque型態轉換能力 10 白色念珠菌野生型SC5314及zcf31∆突變株菌絲長度 11 白色念珠菌野生型SC5314及zcf31∆突變株貼附能力 11 白色念珠菌野生型SC5314及zcf31∆突變株生物膜生成情形 12 檢驗白色念珠菌野生型SC5314及zcf31∆突變株入侵能力 12 白色念珠菌野生型SC5314及zcf31∆突變株被巨噬細胞RAW246.7吞噬的數量 13 動物毒性實驗 13 第四章 實驗結果 14 第一節 探討白色念珠菌zcf31∆突變株對於致病能力的影響 14 一、白色念珠菌ZCF31基因剔除不改變white-opaque型態轉換能力 14 二、刪除ZCF31基因不會改變由小牛血清誘導生成的菌絲長度 14 三、刪除ZCF31基因並不會改變念珠菌的貼附能力 15 四、刪除ZCF31基因念珠菌並不會改變生物膜生成量 16 五、刪除ZCF31基因不會改變念珠菌入侵表皮細胞的能力 17 第二節 探討白色念珠菌zcf31∆突變株對於宿主免疫反應的影響 18 一、刪除ZCF31基因不改變念珠菌被巨噬細胞RAW246.7吞噬的數量 18 二、動物毒性實驗 18 第五章 討論 20 第一節 探討白色念珠菌zcf31∆突變株對於致病能力的影響 20 第二節 探討白色念珠菌zcf31∆突變株對於宿主免疫反應的影響 23 第六章 未來方向 26 第七章 圖表 28 第八章 參考文獻 37 第九章 附錄 48 | |
| dc.language.iso | zh-TW | |
| dc.subject | 白色念珠菌 | zh_TW |
| dc.subject | ZCF31 | zh_TW |
| dc.subject | 致病力 | zh_TW |
| dc.subject | 菌絲生成 | zh_TW |
| dc.subject | 生物膜生成 | zh_TW |
| dc.subject | 吞噬作用 | zh_TW |
| dc.subject | biofilm formation | en |
| dc.subject | phagocytosis | en |
| dc.subject | pathogenic potential | en |
| dc.subject | ZCF31 | en |
| dc.subject | hyphae formation | en |
| dc.subject | Candida albicans | en |
| dc.title | 探討基因ZCF31的缺失在白色念珠菌中對於致病力所造成的影響 | zh_TW |
| dc.title | Examination of the pathogenic potential of the Candida albicans zcf31 deletion strain | en |
| dc.date.schoolyear | 110-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 羅秀容(Hsin-Tsai Liu),呂俊毅(Chih-Yang Tseng),薛雁冰,江皓森 | |
| dc.subject.keyword | 白色念珠菌,ZCF31,致病力,菌絲生成,生物膜生成,吞噬作用, | zh_TW |
| dc.subject.keyword | Candida albicans,ZCF31,pathogenic potential,hyphae formation,biofilm formation,phagocytosis, | en |
| dc.relation.page | 52 | |
| dc.identifier.doi | 10.6342/NTU202103168 | |
| dc.rights.note | 同意授權(限校園內公開) | |
| dc.date.accepted | 2022-02-10 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| 顯示於系所單位： | 生化科技學系 | |
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