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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 顏雪琪(Hsueh-Chi Yen) | |
| dc.contributor.author | Li-Chin Wang | en |
| dc.contributor.author | 王俐晴 | zh_TW |
| dc.date.accessioned | 2022-11-24T03:02:48Z | - |
| dc.date.available | 2021-08-10 | |
| dc.date.available | 2022-11-24T03:02:48Z | - |
| dc.date.copyright | 2021-08-10 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-07-12 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/80224 | - |
| dc.description.abstract | "泛素所引導的蛋白質降解系統（Ubiquitin proteasome system）是主要的蛋白質降解系統之一，它參與細胞功能的各種功能調控。在泛素所引導的蛋白質降解系統中，泛素連接酶藉由專一性地辨認降解訊號來清除蛋白質。我們實驗室過去發現新類別的蛋白質降解訊號，我們稱它蛋白質羧基端降解密碼子（C-degron），當它座落在蛋白質羧基端會被辨認降解。雖然我們過去找到一些羧基端降解密碼子的特徵，但我們無法得知這些特徵是否足夠讓蛋白質降解。為了解決這些問題，我發展了一個高通量和情境獨立（context-independent）的方法。我合成帶有特定特徵的隨機胜肽圖庫並結合以螢光測量蛋白質穩定的高通量技術(Global Protein Stability assay, GPS)來檢驗降解密碼的降解強度。我找到了被 CRL2APPBP2和 CRL2KLHDC3 (泛素連接酶)辨認的羧基端降解密碼的缺失特徵。APPBP2 偏好降解蛋白質羧基端帶有正電或極性的xRxxGx模體(motif, x代表隨機胜肽), 當羧基端xRxxGx模體代有多個正電氨基酸，將近100%隨機胜肽圖庫可被APPBP2辨認降解 。當蛋白質羧基端帶有RxxxKG模體(motif)，將近100%會被KLHDC3辨認降解。GPS隨機胜肽平台使我們能夠簡單且可靠地去廣泛找到降解密碼子的特徵。" | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-24T03:02:48Z (GMT). No. of bitstreams: 1 U0001-1007202113083300.pdf: 6469904 bytes, checksum: 36ac8b69d4dfad8eb2f1575c050cd84f (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 摘要 I ABSTRACT II INTRODUCTION 1 UBIQUITIN-PROTEASOME SYSTEM 1 DEGRONS 2 C-DEGRONS 3 CURRENT METHODS TO IDENTIFY AND CHARACTERIZE DEGRONS 4 GLOBAL PROTEIN STABILITY (GPS) ASSAY 6 THE AIM OF MY THESIS 7 EXPERIMENTAL DESIGN 8 CHARACTERIZATION OF DEGRON POTENCY VIA CONTEXT-INDEPENDENT RANDOM PEPTIDES AND GPS ASSAY 8 ANALYSIS OF DEGRON SEQUENCES BY SINGLE CELL SORTING AND SEQUENCES COMPARISON 9 ASSESSMENT OF DEGRON POTENCY BY DESIGNING DIFFERENT ADD-IN TARGETED FEATURES 10 CALIBRATION AND ANALYSIS OF %DEGRADATION READOUT FROM GPS 11 METHODS AND MATERIALS 14 RESULTS 17 PROTOTYPE DEGRON MOTIFS IN PROTEIN C-TERMINUS ARE INSUFFICIENT FOR APPBP2- AND KLHDC3-MEDIATED DEGRADATION 17 CHARACTERIZATION OF APPBP2 DEGRON BY SINGLE CELL SORTING AND SEQUENCING 18 CHARACTERIZATION MISSING FEATURES OF APPBP2 DEGRON VIA DIFFERENT ADD-IN FEATURES OF RANDOM PEPTIDE PLATFORMS 19 CHARACTERIZATION MISSING FEATURES OF KLHDC3 DEGRON VIA DIFFERENT ADD-IN FEATURES OF RANDOM PEPTIDE PLATFORMS 22 SUMMARY AND DISCUSSION 24 REFERENCES 27 FIGURES AND APPENDICES 30 | |
| dc.language.iso | en | |
| dc.subject | C端降解密碼 | zh_TW |
| dc.subject | 蛋白質降解 | zh_TW |
| dc.subject | 高通量蛋白質穩定度偵測系統 | zh_TW |
| dc.subject | 隨機胜肽平台 | zh_TW |
| dc.subject | random peptide platform | en |
| dc.subject | protein degradation | en |
| dc.subject | C-degron | en |
| dc.subject | Global Protein Stability assay | en |
| dc.title | 蛋白穩定度隨機胜肽平台歸納蛋白質羧基端降解密碼的特性 | zh_TW |
| dc.title | Characterization of C-degrons via the Global Protein Stability Random Peptide Platform | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 鄭貽生(Hsin-Tsai Liu),陳光超(Chih-Yang Tseng) | |
| dc.subject.keyword | 蛋白質降解,C端降解密碼,高通量蛋白質穩定度偵測系統,隨機胜肽平台, | zh_TW |
| dc.subject.keyword | protein degradation,C-degron,Global Protein Stability assay,random peptide platform, | en |
| dc.relation.page | 48 | |
| dc.identifier.doi | 10.6342/NTU202101376 | |
| dc.rights.note | 同意授權(限校園內公開) | |
| dc.date.accepted | 2021-07-12 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 基因體與系統生物學學位學程 | zh_TW |
| 顯示於系所單位： | 基因體與系統生物學學位學程 | |
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