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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林晉玄(Ching-Hsuan Lin) | |
| dc.contributor.author | Yu-De Song | en |
| dc.contributor.author | 宋昱德 | zh_TW |
| dc.date.accessioned | 2022-11-23T09:31:15Z | - |
| dc.date.available | 2021-07-20 | |
| dc.date.available | 2022-11-23T09:31:15Z | - |
| dc.date.copyright | 2021-07-20 | |
| dc.date.issued | 2020 | |
| dc.date.submitted | 2021-06-22 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/80196 | - |
| dc.description.abstract | "NDT80 在真菌當中是一個非常保守的基因，然而Ndt80p卻在不同的物種當中演化出不同的功能。在發酵酵母 (Saccharomyces cerevisiae) 當中Ndt80扮演調控減數分裂的角色，但是在致病真菌白色念珠菌 (Candida albicans) 卻演化出三個 NDT80-like 基因，NDT80、REP1 與 RON1，並在白色念珠菌當中負責調控生物膜生成、藥物抗性 (drug resistance) 與代謝N-乙醯葡醣胺 (N-acetylglucosamine, GlcNAc) 的功能。同樣是屬於CTG支序群 (clade) 的熱帶念珠菌，也擁有三個NDT80-like的同源基因。在台灣非白色念珠菌念珠菌屬 (non-albicans Candida spp.) 在院內感染的情形日益嚴重，而最盛行的即為熱帶念珠菌 (Candida tropicalis)。在本研究中將著重於探討熱帶念珠菌RON1 所扮演的功能。透過序列比對， CtRon1 與 CaRon1 僅具有39.7% 的保守性 (identity)，而在以N-乙醯葡醣胺為碳源的培養基上，熱帶念珠菌 ron1∆ 突變株仍能正常生長，且不參與調控代謝GlcNAc的相關基因。然而，實驗結果顯示，REP1 與GlcNAc 代謝有關。在菌絲調控上，熱帶念珠菌ron1∆ 突變株沒辦法透過血清誘導生成菌絲，同時也會造成生物膜形成的缺失。有趣的是，rep1∆ 在N-乙醯葡醣胺的刺激下，會促使菌絲生成，有別於N-乙醯葡醣胺對於熱帶念珠菌野生株所扮演之抑制菌絲生成的角色。最後利用小鼠觀察RON1是否影響熱帶念珠菌的致病能力，結果顯示，ron1∆ 突變株的致死率大幅降低。說明在熱帶念珠菌當中，RON1 參與調控菌絲、生物膜生成以及影響對小鼠的致病力。" | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-23T09:31:15Z (GMT). No. of bitstreams: 1 U0001-2006202115035300.pdf: 9539615 bytes, checksum: 7f6b499ccedc4c63a9979d68c0bf7658 (MD5) Previous issue date: 2020 | en |
| dc.description.tableofcontents | 目錄 摘要 I Abstract II 目錄 IV 表目錄 VI 圖目錄 VII 第一章 前言 1 壹、念珠菌與臨床感染症狀 1 貳、臺灣院內念珠菌感染現況 2 參、念珠菌之菌絲生成機制 3 肆、念珠菌之生物膜生成機制 5 伍、類Ndt80 蛋白 (Ndt80-like protein)之介紹 7 陸、N-乙醯葡醣胺調控念珠菌之型態發生 9 第二章 實驗目的 12 第三章 材料與方法 13 第四章 結果 19 壹、比較熱帶念珠菌Ron1、白色念珠菌Ron1與發酵酵母Ndt80胺基酸序列之差異 19 貳、剔除RON1在不同碳源之培養基仍能正常生長 19 參、系統發生樹顯示念珠菌屬之 Rep1 較為接近T. reesei 之 Ron1 20 肆、rep1∆ 突變株在以 GlcNAc 或 Galactose 為碳源的培養基生長受限 21 伍、ron1∆ 在血清誘導條件下，無法生成菌絲。而 rep1∆ 在 GlcNAc 誘導下能刺激菌絲生長 21 陸、剔除 RON1 造成生物膜生成下降 23 柒、利用ICR小鼠動物模型探討C. tropicalis RON1 對致病力之影響 24 第五章 討論 26 第六章 未來研究方向 31 第七章 圖表 32 第八章 參考文獻 49 第九章 附錄 59 附錄一 本實驗室培養基配方 59 附錄二 突變株構築方法與鑑定 61 | |
| dc.language.iso | zh-TW | |
| dc.subject | 致病力 | zh_TW |
| dc.subject | NDT80-like | zh_TW |
| dc.subject | 熱帶念珠菌 | zh_TW |
| dc.subject | 菌絲 | zh_TW |
| dc.subject | 生物膜 | zh_TW |
| dc.subject | RON1 | zh_TW |
| dc.subject | Ron1 | en |
| dc.subject | NDT80-like gene | en |
| dc.subject | Candida tropicalis | en |
| dc.subject | hyphal growth | en |
| dc.subject | biofilms | en |
| dc.subject | virulence | en |
| dc.title | 探討 RON1 於熱帶念珠菌當中所扮演之功能 | zh_TW |
| dc.title | Investigation of the function of RON1 in Candida tropicalis | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 孫培倫(Hsin-Tsai Liu),羅秀容(Chih-Yang Tseng),陳進庭,呂俊毅,薛雁冰 | |
| dc.subject.keyword | 熱帶念珠菌,NDT80-like,RON1,菌絲,生物膜,致病力, | zh_TW |
| dc.subject.keyword | Candida tropicalis,,NDT80-like gene,Ron1,hyphal growth,biofilms,virulence, | en |
| dc.relation.page | 61 | |
| dc.identifier.doi | 10.6342/NTU202101065 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2021-06-22 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| 顯示於系所單位： | 生化科技學系 | |
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