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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 楊瑋誠(Wei-Cheng Yang) | |
| dc.contributor.author | Chung-Hei Wong | en |
| dc.contributor.author | 黃頌睎 | zh_TW |
| dc.date.accessioned | 2022-11-23T09:26:11Z | - |
| dc.date.available | 2022-09-01 | |
| dc.date.available | 2022-11-23T09:26:11Z | - |
| dc.date.copyright | 2021-08-18 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-07-09 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/80102 | - |
| dc.description.abstract | 近年來,在公眾對動物福利的關注由農場擴大至動物園及水族館,令福利科學的研究日益增長。在圈養動物中,壓力及其來源是福利問題的重點。由於壓力可以反映動物福利的不利影響,因此評估動物壓力的客觀方法將是必不可少的。使用行為和生理參數作為指標來定量評估動物福利,而不是簡單地定性評估,可以防止歸因偏差的發生,並且成為比單獨行為測量更有效的緊迫和動物福利指標。為了驗證這種方法,將測量台灣圈養白鯨 (Delphinapterus leucas) 聲學活動和皮屑皮質醇濃度作為識別緊迫因子方法。三頭圈養白鯨的聲學活動 (每小時 5 分鐘) 已由傳感器常規記錄,並使用音頻編輯軟件進行分析。根據已發表論文,白鯨的叫聲分為三大類,分別為口哨聲、脈沖聲和組合叫聲。每週於白鯨胸鰭及/或尾鰭非侵入性地刮取皮屑樣本一次。改良已發表論文中皮膚固醇類提取技術以提取皮質醇,並通過市售酶免疫測定法進行檢測。發現兩個採樣位置 (胸鰭及尾鰭) 的皮質醇濃度相似。通過使用評分方法分析從事件到皮屑皮質醇的時間滯後,注意到68-72天得分最高,說明白鯨的皮膚皮質醇可能代表2個月前出現的應激反應。應用時間滯後後,根據飼養和醫療記錄選擇事件的日期。三個具有高質量音檔的事件被用來進行壓力反應的分析。在每個事件中至少可觀察到一天的呼叫聲學活動減少。另外,雖要三頭白鯨的皮質醇濃度沒有顯示出統計學差異,但它們的皮質醇濃度各不相同。在不同個體中發現了對壓力的不同反應,表明動物的性格可以表現在皮屑皮質醇濃度的變化中。結果顯示,由於皮質醇濃度和聲學行為的變化可以反映環境和身體狀況的波動,因此測量皮屑皮質醇和記錄聲學活動是一種監測白鯨壓力水平的有價值方法。這項研究為圈養鯨類壓力和福利科學研究的提供基礎信息,可用於監測並隨後改善它們的生活質量,並將有助維護圈養鯨豚之動物福利並提高牠們的生活品質。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-23T09:26:11Z (GMT). No. of bitstreams: 1 U0001-0807202115391200.pdf: 1893606 bytes, checksum: 70bf5e2fc05ca679d5caed8308ca2c83 (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 中⽂摘要 i Abstract ii Section 1 Introduction 1 1.1 Behavioral and Physiological assessment in welfare 1 1.2 Stress and welfare 3 1.3 Welfare assessment in captive cetaceans 4 1.4 Acoustic activities in beluga whales 6 1.5 Cortisol studies in cetaceans 7 1.6 Cortisol detection methods 9 1.7 Aim of study 10 Section 2 Material and method 11 2.1 Scrape sample collection 11 2.2 Hormone extraction 12 2.3 Cortisol detection. 13 2.4 Parallelism and matrix effects analyses 14 2.5 Husbandry and medical records collection 15 2.6 Scoring method 15 2.7 Acoustic activities 16 2.8 Statistical analysis 17 Section 3 Results 19 3.1 EIA performance assessments 19 3.2 Scrape cortisol 20 3.3 Acoustic activities 22 Section 4 Discussion 25 Section 5 Reference 35 Tables: Table. 1 Days of time lag and scores 21 Figure: Figure 1 Cross-section of cetacean skin 12 Figure 2 Routine monitor sampling sites of beluga whales 12 Figure 3 The floor plan of the aquarium 17 Figure 4 Results from linearity assessment of EIA standard with scraped extracts19 Figure 5 Linear regression of flipper-fluke cortisol concentration 21 Figure 6 Box plot of scrape cortisol concentration 21 Figure 7 Line graph of scrape cortisol concentration 22 Figure 8 The total number of calls and the pattern of acoustic activities in the analyzed events 23 Figure 9 Results of PCA analysis of different events 24 | |
| dc.language.iso | en | |
| dc.subject | 皮質醇 | zh_TW |
| dc.subject | 白鯨 | zh_TW |
| dc.subject | 緊迫 | zh_TW |
| dc.subject | 動物福利 | zh_TW |
| dc.subject | 聲學活動 | zh_TW |
| dc.subject | Animal welfare | en |
| dc.subject | Beluga whales | en |
| dc.subject | Acoustic activities | en |
| dc.subject | Cortisol | en |
| dc.subject | Stress | en |
| dc.title | 測量圈養白鯨聲學活動及皮屑皮質醇作為識別緊迫因子方法 | zh_TW |
| dc.title | Measuring Acoustic Activity and Scrape Cortisol as A Method to Identify Stressors in Captive Beluga Whales (Delphinapterus leucas) | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳德豪(Hsin-Tsai Liu),蔡明安(Chih-Yang Tseng),葛展榮 | |
| dc.subject.keyword | 白鯨,聲學活動,皮質醇,緊迫,動物福利, | zh_TW |
| dc.subject.keyword | Beluga whales,Acoustic activities,Cortisol,Stress,Animal welfare, | en |
| dc.relation.page | 45 | |
| dc.identifier.doi | 10.6342/NTU202101345 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2021-07-11 | |
| dc.contributor.author-college | 獸醫專業學院 | zh_TW |
| dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
| 顯示於系所單位: | 獸醫學系 | |
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