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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林乃君(Nai-Chun Lin) | |
| dc.contributor.author | Jian-Hong Lin | en |
| dc.contributor.author | 林建宏 | zh_TW |
| dc.date.accessioned | 2022-11-23T09:25:24Z | - |
| dc.date.available | 2021-07-20 | |
| dc.date.available | 2022-11-23T09:25:24Z | - |
| dc.date.copyright | 2021-07-20 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-07-12 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/80087 | - |
| dc.description.abstract | " 高科技產業日漸發展，為人類生活帶來更多便利，然而伴隨而來的可能會有新興汙染物的汙染，如鎵和銦。先前研究調查發現新竹科學園區之排放廢水含有 0.05~10.0 μg L-1 的銦。前人研究注重在銦對動物與植物的影響，然而對微生物之影響研究反而不多，故我們想從銦是否對微生物有毒害，及土壤中是否具有耐銦之微生物開始了解，繼而利用水稻幼苗建立之接近無菌栽培系統來探討這些微生物對植體中銦轉運之影響。利用增殖培養之方式，在平鎮與三坑子土系中共分離到 59 支能夠抗鎵、銦或鉈能力之菌株，且其中多株菌株具有能夠同時耐受兩種以上重金屬之能力；再透過培養基測試各菌株之促進植物生長 (plant growth-promoting, PGP) 能力，發現有 23 支菌具螯合鐵之能力， 10 支菌具有溶磷能力，而 36 支菌具有降解尿素之能力，但僅有 5 支菌可產生植物荷爾蒙中的吲哚乙酸 (indole acetic acid, IAA)。從銦轉運效率上升的水稻植株根部分離根圈菌與內生菌，一共分離出 9 支根圈菌和 2 支內生菌，其中 In200/SE R-2 與 In200/SE R-6 具有產生鐵載體之螯合鐵能力；In200/SE R-3、In200/SE R-4 與 In50/SE R-2 具有溶磷之能力；In200/SE R-2、In200/SE R-6、In50/SE R-1 與 In50/SE R-2 具有產生尿素酶之能力；In200/SE R-5、In200/SE R-6、In50/SE E-1、 In50/SE E-2 與 In50/SE R-1 對 200 mg/L 之銦有良好耐受性。在開蓋近無菌土壤系統中，添加Bacillus sp. E1 於土壤中且在 50 mg/L 之銦處理下，可以使水稻之地上部銦濃度累積量增加，從轉運係數 (translocation factor, TF) 值來看，In50/E1 組之 TF 值為 0.072 較 In50 組之 TF 值 0.037 來的高，表示 E1 具有增加銦在水稻中轉運之能力。除此之外，Bacillus sp. E1 菌體能夠吸附銦，量可達到約 8.37 mg/g cells。然而，E1 對水稻植體中銦之轉運增加的實際機制為何以及吸附銦的機制為何，仍需等待進一步之研究探討。本研究發現土壤中具有能夠幫助植物將銦轉運到地上部，且能吸附銦的細菌，未來應可將此菌株用於發展提升植生復育效率上，使植物吸收更多重金屬並轉運到地上部；此外，也可用在銦之回收利用，減少為了銦而過度開採造成環境之破壞。 " | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-23T09:25:24Z (GMT). No. of bitstreams: 1 U0001-1107202101530800.pdf: 1908855 bytes, checksum: ce924135ab730a5b3b4a59d267e55b01 (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | "目錄 誌謝 i 摘要 iii 表目錄 vii 圖目錄 viii 壹、前人研究 1 一、新興汙染物的定義與介紹 1 二、鎵 2 (一) 鎵的特性與應用 2 (二) 鎵對動物之影響 2 (三) 鎵對人體之影響 4 (四) 鎵對植物之影響 5 三、銦 5 (一) 銦的特性與應用 5 (二) 銦對動物之影響 7 (三) 銦對人體之影響 8 (四) 銦對植物之影響 8 四、鉈 9 (一) 鉈的特性與應用 9 (二) 鉈對動物之影響 10 (三) 鉈對人體之影響 10 (四)鉈對植物之影響 11 五、微生物對重金屬之耐受機制 12 六、微生物影響重金屬對植物之毒害 12 貳、研究動機與目的 15 參、實驗設計與架構 16 肆、材料與方法 17 一、土壤採樣 17 二、土壤性質分析 17 pH 值測定 17 電導度測定 (Electrical conductivity, EC) 17 陽離子交換能力測定 (Cation exchange capacity, CEC) 17 有機碳測定 (total organic carbon, TOC) 18 可交換性鋁、鎵、銦和鉈測定分析 (Exchangeable aluminum, gallium, indium and thallium) 19 三、分離平鎮土系與三坑子土系中耐鎵、銦或鉈的細菌 19 四、耐鎵、銦或鉈細菌的篩選 20 五、促進植物生長特性分析 20 溶磷能力分析 20 產生鐵載體能力分析 20 分解尿素能力分析 21 吲哚乙酸合成能力分析 21 六、水稻育苗與未育苗之比較試驗 21 七、微生物對水稻耐受銦影響之試驗 22 八、分離水稻根圈細菌與內生細菌 23 九、水稻根圈細菌與內生細菌耐受銦能力測定 23 十、Genomic DNA 的萃取 24 十一、16S rDNA 序列分析 24 十二、植體消化與分析 25 十三、細菌吸附銦能力測定 25 十四、最小抑制濃度測試 (Minimum Inhibitory Concentration, MIC) 26 十五、統計分析 26 伍、結果 27 一、平鎮系土壤和三坑子系土壤之土壤性質 27 二、平鎮土系土壤和三坑子土系土壤中具有能夠耐受鎵、銦、鉈之微生物 27 三、平鎮土系土壤和三坑子土系土壤中的微生物具有 PGP 能力，包括溶磷、螯鐵、尿素酶和合成 IAA能力等 28 四、隨著添加銦濃度增加，水稻根部銦之累積量亦增加 29 五、無菌系統中添加微生物對不同銦濃度處理水稻生長之影響 30 六、添加土壤混合菌液可以使水稻植體地上部之銦累積濃度增加 30 七、水稻根圈細菌與內生細菌之分離與其對銦耐受性和 PGP 能力測定 31 八、根圈細菌與內生細菌之菌種鑑定 31 九、在有蓋無菌系統下，E2對水稻之生長有負面影響 32 十、在開蓋無菌土壤系統中，E1 可以使水稻植體之地上部銦濃度上升，增加銦從根部送往莖部 33 十一、內生菌 E1 對銦具有吸附能力 33 十二、銦對 E1 之最小抑制濃度 (MIC) 33 陸、討論 34 柒、參考文獻 39 扒、表 50 玖、圖 59 " | |
| dc.language.iso | zh-TW | |
| dc.subject | 水稻 | zh_TW |
| dc.subject | 銦 | zh_TW |
| dc.subject | 耐銦細菌 | zh_TW |
| dc.subject | 轉運因子 | zh_TW |
| dc.subject | Indium | en |
| dc.subject | Oryza sativa | en |
| dc.subject | Indium-tolerant bacterium | en |
| dc.subject | Translocation factor | en |
| dc.title | 耐銦細菌對水稻幼苗中銦轉運之影響 | zh_TW |
| dc.title | Effect of the indium-tolerant bacteria on the translocation of indium in rice seedlings | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 許正一(Hsin-Tsai Liu),陳佩貞(Chih-Yang Tseng),林雅芬 | |
| dc.subject.keyword | 銦,水稻,耐銦細菌,轉運因子, | zh_TW |
| dc.subject.keyword | Indium,Oryza sativa,Indium-tolerant bacterium,Translocation factor, | en |
| dc.relation.page | 64 | |
| dc.identifier.doi | 10.6342/NTU202101384 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2021-07-13 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 農業化學研究所 | zh_TW |
| 顯示於系所單位： | 農業化學系 | |
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