請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79886完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 孫珍理(Chen-li Sun) | |
| dc.contributor.author | Shu-Qi Liu | en |
| dc.contributor.author | 劉書齊 | zh_TW |
| dc.date.accessioned | 2022-11-23T09:15:20Z | - |
| dc.date.available | 2021-08-17 | |
| dc.date.available | 2022-11-23T09:15:20Z | - |
| dc.date.copyright | 2021-08-17 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-08-12 | |
| dc.identifier.citation | [1] D. M. Karl, E. A. Laws, P. Morris, and S. Emerson, 'Metabolic balance of the open sea,' Nature, vol. 426, no. 6962, pp. 32-32, 2003. (10.1038/426032a) [2] R. J. Geider, E. H. Delucia, P. G. Falkowski, A. C. Finzi, J. P. Grime, and J. Grace, 'Primary productivity of planet earth: biological determinants and physical constraints in terrestrial and aquatic habitats,' Global Change Biology, vol. 7, no. 8, pp. 849-882, 2001. [3] B. P. Durham, S. Sharma, H. Luo, C. B. Smith, S. A. Amin, S. J. Bender, S. P. Dearth, B. A. S. Van Mooy, S. R. Campagna, and E. B. Kujawinski, 'Cryptic carbon and sulfur cycling between surface ocean plankton,' Proceedings of the National Academy of Sciences, vol. 112, no. 2, pp. 453-457, 2015. (10.1073/pnas.1413137112) [4] T. L. Hamilton, D. A. Bryant, and J. L. Macalady, 'The role of biology in planetary evolution: cyanobacterial primary production in low‐oxygen Proterozoic oceans,' Environmental Microbiology, vol. 18, no. 2, pp. 325-340, 2016. (10.1111/1462-2920.13118) [5] P. Sánchez-Baracaldo, 'Origin of marine planktonic cyanobacteria,' Scientific Reports, vol. 5, no. 1, pp. 1-10, 2015. (10.1038/srep17418) [6] W. Copenhagen, 'The periodic mortality of fish in the Walvis region,' South African Medical Journal, vol. 28, no. 18, p. 381, 1954. [7] J. W. Kempton, A. J. Lewitus, J. R. Deeds, J. M. Law, and A. R. Place, 'Toxicity of Karlodinium micrum (Dinophyceae) associated with a fish kill in a South Carolina brackish retention pond,' Harmful Algae, vol. 1, no. 2, pp. 233-241, 2002. (10.1016/S1568-9883(02)00015-X) [8] G. M. Hallegraeff, 'A review of harmful algal blooms and their apparent global increase,' Phycologia, vol. 32, no. 2, pp. 79-99, 1993. (10.2216/i0031-8884-32-2-79.1) [9] J. Heisler, P. M. Glibert, J. M. Burkholder, D. M. Anderson, W. Cochlan, W. C. Dennison, Q. Dortch, C. J. Gobler, C. A. Heil, and E. Humphries, 'Eutrophication and harmful algal blooms: a scientific consensus,' Harmful Algae, vol. 8, no. 1, pp. 3-13, 2008. (10.1016/j.hal.2008.08.006) [10] D. M. Anderson, J. M. Burkholder, W. P. Cochlan, P. M. Glibert, C. J. Gobler, C. A. Heil, R. M. Kudela, M. L. Parsons, J. J. Rensel, D. W. Townsend, V. L. Trainer, and G. A. Vargo, 'Harmful algal blooms and eutrophication: examining linkages from selected coastal regions of the United States,' Harmful Algae, vol. 8, no. 1, pp. 39-53, 2008. (10.1016/j.hal.2008.08.017) [11] S. Sakamoto, W. A. Lim, D. Lu, X. Dai, T. Orlova, and M. Iwataki, 'Harmful algal blooms and associated fisheries damage in East Asia: Current status and trends in China, Japan, Korea and Russia,' Harmful Algae, p. 101787, 2020. (10.1016/j.hal.2020.101787) [12] J. R. Deeds, D. E. Terlizzi, J. E. Adolf, D. K. Stoecker, and A. R. Place, 'Toxic activity from cultures of Karlodinium micrum (= Gyrodinium galatheanum)(Dinophyceae)—a dinoflagellate associated with fish mortalities in an estuarine aquaculture facility,' Harmful Algae, vol. 1, no. 2, pp. 169-189, 2002. (10.1016/S1568-9883(02)00027-6) [13] J. O. Kessler, 'Hydrodynamic focusing of motile algal cells,' Nature, vol. 313, no. 5999, pp. 218-220, 1985. (10.1038/313218a0) [14] W. M. Durham, J. O. Kessler, and R. Stocker, 'Disruption of vertical motility by shear triggers formation of thin phytoplankton layers,' Science, vol. 323, no. 5917, pp. 1067-1070, 2009. (10.1126/science.1167334) [15] C. Adams and A. Paul, 'Phototaxis and geotaxis of light-adapted zoeae of the golden king crab Lithodes Aequispinus (anomura: Lithodidae) in the laboratory,' Journal of Crustacean Biology, vol. 19, no. 1, pp. 106-110, 1999. (10.1163/193724099X00303) [16] S. I. Dodson, S. Ryan, R. Tollrian, and W. Lampert, 'Individual swimming behavior of Daphnia: effects of food, light and container size in four clones,' Journal of plankton research, vol. 19, no. 10, pp. 1537-1552, 1997. (10.1093/plankt/19.10.1537) [17] S. I. Dodson, R. Tollrian, and W. Lampert, 'Daphnia swimming behavior during vertical migration,' Journal of plankton research, vol. 19, no. 8, pp. 969-978, 1997. (10.1093/plankt/19.8.969) [18] A. R. Place, H. A. Bowers, T. R. Bachvaroff, J. E. Adolf, J. R. Deeds, and J. Sheng, 'Karlodinium veneficum—The little dinoflagellate with a big bite,' Harmful Algae, vol. 14, pp. 179-195, 2012. (10.1016/j.hal.2011.10.021) [19] N. Daugbjerg, G. Hansen, J. Larsen, and Ø. Moestrup, 'Phylogeny of some of the major genera of dinoflagellates based on ultrastructure and partial LSU rDNA sequence data, including the erection of three new genera of unarmoured dinoflagellates,' Phycologia, vol. 39, no. 4, pp. 302-317, 2000. (10.2216/i0031-8884-39-4-302.1) [20] T. Bergholtz, N. Daugbjerg, Ø. Moestrup, and M. Fernández‐Tejedor, 'On the identity of Karlodinium veneficum and description of Karlodinium armiger SP.Nov. (Dinophyceae), based on lift and electron microscopy, nuclear-encoded LSU rDNS, and pigment composition,' Journal of Phycology, vol. 42, no. 1, pp. 170-193, 2006. (10.1111/j.1529-8817.2006.00172.x) [21] J. Sheng, E. Malkiel, J. Katz, J. Adolf, R. Belas, and A. R. Place, 'Digital holographic microscopy reveals prey-induced changes in swimming behavior of predatory dinoflagellates,' Proceedings of the National Academy of Sciences, vol. 104, no. 44, pp. 17512-17517, 2007. (10.1073/pnas.0704658104) [22] J. Sheng, E. Malkiel, J. Katz, J. E. Adolf, and A. R. Place, 'A dinoflagellate exploits toxins to immobilize prey prior to ingestion,' Proceedings of the National Academy of Sciences, vol. 107, no. 5, pp. 2082-2087, 2010. (10.1073/pnas.0912254107) [23] R. J. Waggett, P. A. Tester, and A. R. Place, 'Anti-grazing properties of the toxic dinoflagellate Karlodinium veneficum during predator–prey interactions with the copepod Acartia tonsa,' Marine Ecology Progress Series, vol. 366, pp. 31-42, 2008. (10.3354/meps07518) [24] J. E. Adolf, D. K. Stoecker, and L. W. Harding Jr, 'Autotrophic growth and photoacclimation in Karlodinium micrum (Dinophyceae) and Storeatula major (Cryptophyceae),' Journal of Phycology, vol. 39, no. 6, pp. 1101-1108, 2003. (10.1111/j.0022-3646.2003.02-086.x) [25] A. Li, D. K. Stoecker, and J. E. Adolf, 'Feeding, pigmentation, photosynthesis and growth of the mixotrophic dinoflagellate Gyrodinium galatheanum,' Aquatic Microbial Ecology, vol. 19, no. 2, pp. 163-176, 1999. (10.3354/ame019163) [26] J. R. Deeds, S. R. Kibler, P. A. Tester, and A. R. Place, 'Geographic strain variation in toxin production in Karlodinium micrum (Dinophyceae) from Southeastern estuaries of the United States,' Harmful Algae, vol. 11, no. 4, pp. 145-147, 2002. [27] J. E. Adolf, T. Bachvaroff, and A. R. Place, 'Can cryptophyte abundance trigger toxic Karlodinium veneficum blooms in eutrophic estuaries?,' Harmful Algae, vol. 8, no. 1, pp. 119-128, 2008. (10.1016/j.hal.2008.08.003) [28] J. E. Adolf, D. Krupatkina, T. Bachvaroff, and A. R. Place, 'Karlotoxin mediates grazing by Oxyrrhis marina on strains of Karlodinium veneficum,' Harmful Algae, vol. 6, no. 3, pp. 400-412, 2007. (10.1016/j.hal.2006.12.003) [29] J. E. Adolf, C. L. Yeager, W. D. Miller, M. E. Mallonee, and L. W. Harding Jr, 'Environmental forcing of phytoplankton floral composition, biomass, and primary productivity in Chesapeake Bay, USA,' Estuarine, Coastal and Shelf Science, vol. 67, no. 1-2, pp. 108-122, 2006. (10.1016/j.ecss.2005.11.030) [30] D. K. Stoecker, J. E. Adolf, A. R. Place, P. M. Glibert, and D. W. Meritt, 'Effects of the dinoflagellates Karlodinium veneficum and Prorocentrum minimum on early life history stages of the eastern oyster (Crassostrea virginica),' Marine Biology, vol. 154, no. 1, pp. 81-90, 2008. (10.1007/s00227-007-0901-z) [31] M. M. Dekshenieks, P. L. Donaghay, J. M. Sullivan, J. E. Rines, T. R. Osborn, and M. S. Twardowski, 'Temporal and spatial occurrence of thin phytoplankton layers in relation to physical processes,' Marine Ecology Progress Series, vol. 223, pp. 61-71, 2001. (10.3354/meps223061) [32] P. J. Franks, 'Thin layers of phytoplankton: a model of formation by near-inertial wave shear,' Deep Sea Research Part I: Oceangraphic Research Papers, vol. 42, no. 1, pp. 75-91, 1995(10.1016/0967-0637(94)00028-Q). [33] M. Mashayekhpour, C. Marchioli, S. Lovecchio, E. N. Lay, and A. Soldati, 'Wind effect on gyrotactic micro-organism surfacing in free-surface turbulence,' Advances in Water Resources, vol. 129, pp. 328-337, 2019. (10.1016/j.advwatres.2017.09.001) [34] D. M. Fields and J. Yen, 'The escape behaviour of Pleuromamma xiphias in response to a quantifiable fluid mechanical disturbance,' in Zooplankton: Snesory Ecology and Physiology, vol. 1: CRC Press, 1996, pp. 323-340. [35] D. M. Fields and J. Yen, 'The escape behavior of marine copepods in response to a quantifiable fluid mechanical disturbance,' Journal of Plankton Research, vol. 19, no. 9, pp. 1289-1304, 1997. (10.1093/plankt/19.9.1289) [36] C. J. Bradley, J. R. Strickler, E. J. Buskey, and P. H. Lenz, 'Swimming and escape behavior in two species of calanoid copepods from nauplius to adult,' Journal of plankton research, vol. 35, no. 1, pp. 49-65, 2013. (10.1093/plankt/fbs088) [37] C.-N. Xu, M. Akiyama, K. Nonaka, and T. Watanabe, 'Electrical power generation characteristics of PZT piezoelectric ceramics,' IEEE Transactions on Ultrasonics, Ferroelectrics, Frequency Control, vol. 45, no. 4, pp. 1065-1070, 1998. (10.1109/58.710589) [38] C. Schabmueller, M. Koch, M. Mokhtari, A. Evans, A. Brunnschweiler, and H. Sehr, 'Self-aligning gas/liquid micropump,' Journal of Micromechanics and Microengineering, vol. 12, no. 4, p. 420, 2002. [39] P. Ge and M. Jouaneh, 'Modeling hysteresis in piezoceramic actuators,' Precision engineering, vol. 17, no. 3, pp. 211-221, 1995. (10.1016/0141-6359(95)00002-U) [40] K. Liu et al., 'Investigation of PZT driven tunable optical filter nonlinearity using FBG optical fiber sensing system,' Optics communications, vol. 281, no. 12, pp. 3286-3290, 2008. (10.1016/j.optcom.2008.02.034) [41] B. Husband, M. Bu, A. G. Evans, and T. Melvin, 'Investigation for the operation of an integrated peristaltic micropump,' Journal of Micromechanics and Microengineering, vol. 14, no. 9, p. S64, 2004. [42] Y. Luo, M. Lu, and T. Cui, 'A polymer-based bidirectional micropump driven by a PZT bimorph,' Microsystem technologies, vol. 17, no. 3, pp. 403-409, 2011. (10.1007/s00542-010-1199-1) [43] M. Rossi, R. Segura, C. Cierpka, and C. J. Kähler, 'On the effect of particle image intensity and image preprocessing on the depth of correlation in micro-PIV,' Experiments in Fluids, vol. 52, no. 4, pp. 1063-1075, 2012. (10.1007/s00348-011-1194-z) [44] C. J. Bourdon, M. G. Olsen, and A. D. Gorby, 'The depth of correlation in micro-PIV for high numerical aperture and immersion objectives,' 2006. (10.1115/1.2201649) [45] W. Thielicke and E. Stamhuis, 'PIVlab–towards user-friendly, affordable and accurate digital particle image velocimetry in MATLAB,' Journal of open research software, vol. 2, no. 1, 2014. (10.5334/jors.bl) [46] R. Goldstein, Fluid Mechanics Measurements. Philadelphia: Taylor Francis, 2017. [47] J. G. Santiago, S. T. Wereley, C. D. Meinhart, D. Beebe, and R. J. Adrian, 'A particle image velocimetry system for microfluidics,' Experiments in Fluids, vol. 25, no. 4, pp. 316-319, 1998. (10.1007/s003480050235) [48] M. E. Erdoğan and C. E. İmrak, 'Effects of the side walls on starting flows in ducts,' International Journal of non-linear mechanics, vol. 40, no. 1, pp. 103-111, 2005. (10.1016/j.ijnonlinmec.2004.05.015) [49] J. Schindelin, I. Arganda-Carreras, E. Frise, V. Kaynig, M. Longair, T. Pietzsch, S. Preibisch, C. Rueden, S. Saalfeld, B. Schmid, J-Y. Tinevez, D. J. White, V. Hartenstein, K. Eliceiri, P. Tomancak, and A. Cardona, 'Fiji: an open-source platform for biological-image analysis,' Nature Methods, Perspective vol. 9, p. 676, 06/28/online 2012. (10.1038/nmeth.2019) [50] MATLAB, version 9.5 (R2018b). The MathWorks Inc., 2009. [51] N. Otsu, 'A threshold selection method from gray-level histograms,' IEEE Transactions on System Man and Cybernetics, vol. 9, no. 1, pp. 62-66, 1979. [52] J.-Y. Tinevez et al., 'TrackMate: An open and extensible platform for single-particle tracking,' Methods, vol. 115, pp. 80-90, 2017. (10.1016/j.ymeth.2016.09.016) [53] D. G. Lowe, 'Distinctive image features from scale-Invariant keypoints,' International Journal of Computer Vision, vol. 60, no. 2, pp. 91-110, 2004/11/01 2004. (10.1023/B:VISI.0000029664.99615.94) [54] C. Loudon and A. Tordesillas, 'The use of the dimensionless Womersley number to characterize the unsteady nature of internal flow,' Journal of theoretical biology, vol. 191, no. 1, pp. 63-78, 1998.(10.1006/.1997.0564) | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79886 | - |
| dc.description.abstract | "本研究探討浮游生物 Karlodinium venificum (CCMP426) 對於不同電壓、頻率驅動之流場的暫態運動行為反應。我們製作一壓電制動之微擾流元件，利用PIV量測震動期間T型流道交會處之流場，另將浮游生物放入微流元件中，獲得震動前、震動期間以及震動結束後浮游生物游泳速度之機率密度函數 (Probability Density Function, PDF) 分布。根據PIV之量測結果，將震動期間的流場分為高擾區(large-fluctuation region)與低擾區(small-fluctuation region)，以歸納位於不同擾動區域的浮游生物游泳速度之變化。 實驗結果顯示，浮游生物之平均游泳速度在震動初期時因受擾動流場刺激而上升，在震動末期時降低，而在關閉震盪源的3分鐘後回復到震動前90%的平均游泳速度。在驅動頻率為4 Hz至20 Hz間，震動初期時浮游生物游泳速度之PDF會在速度為40 um s-1至100 um s-1之間形成一平坦高原之分布，代表4 Hz至20 Hz之震動流場對浮游生物運動速度之影響極為明顯。另在驅動頻率為20 Hz時，觀察到關閉震盪源會再形成流場改變的刺激，導致浮游生物於震盪源關閉時在游泳速度為100 um s-1至140 um s-1的高游泳速度區多出一個稍低的PDF峰值。此外，於相同震動階段下，位在高擾區的浮游生物之平均游泳速度皆高於位在低擾區之值，代表浮游生物對於擾動速度較高的環境會有較快游泳速度的反應。而在驅動頻率為20 Hz 至40 Hz間，位在低擾區的浮游生物會先在震動初期降低游泳速度，並在震動末期提升其游泳速度，但不會高於震動前之值；但位在高擾區的浮游生物會於震動初期即提高游泳速度，而在震動末期降低其游泳速度。在驅動頻率為200 Hz至400 Hz間，流場平均擾動速度為20 Hz至40 Hz之值的10至50倍，位於高擾區及低擾區的浮游生物皆會在震動初期受擾動流場刺激提高游泳速度，而在震動末期降低游泳速度。 " | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-23T09:15:20Z (GMT). No. of bitstreams: 1 U0001-3007202114135300.pdf: 11794884 bytes, checksum: 9d7d2d53595a4ff44063bff98dfeb82c (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 第一章 導論 1 1.1 前言 1 1.2 文獻回顧 2 1.2.1 Karlodinium veneficum 2 1.2.2浮游微生物在受擾動流場下之反應 4 1.2.3 壓電效應與其在微流元件中的運用 5 1.3 研究動機 5 第二章 實驗架構與不確定性分析 6 2.1 微流元件製作概述 6 2.1.1擾動產生裝置之製作 6 2.1.2 T型微流道 7 2.1.3元件黏合 8 2.2 實驗架構 8 2.2.1 流體驅動系統 8 2.2.2 光學影像系統 9 2.2.3 mPIV量測系統 9 2.3 實驗程序 11 2.3.1 mPIV速度場校正 11 2.3.2 K.veneficum之培養 13 2.3.3 浮游生物之濃縮 13 2.3.4 實驗步驟 15 2.4 影像分析 17 2.4.1 除去背景與灰階值正規化 17 2.4.2 浮游生物追蹤程序 17 2.4.3 浮游生物之運動行為分析程序 18 2.5 不確定性分析 19 2.5.1 流場速度量測之不確定性 20 2.5.2 K.veneficum 位移之不確定性 21 2.5.3 K.veneficum 速度之不確定性 21 2.5.4 K.veneficum 轉彎角度之不確定性 21 2.5.5 PDF之不確定性 22 第三章 實驗結果 24 3.1 mPIV量測結果 24 3.1.1 驅動頻率2~4 Hz之流場特徵 24 3.1.2 驅動頻率20~40 Hz之流場特徵 26 3.1.3 驅動頻率200~400 Hz之流場特徵 27 3.2 K.veneficum於驅動頻率2~4 Hz之運動分析 29 3.2.1 驅動頻率2 Hz下K.veneficum之運動分析 29 3.2.2 驅動頻率4 Hz下K.veneficum之運動分析 31 3.3 K.veneficum於驅動頻率20~40 Hz之運動分析 33 3.3.1 驅動頻率為20 Hz下K.veneficum之運動分析 33 3.3.2 驅動頻率為40 Hz下K.veneficum之運動分析 36 3.4 K.veneficum於驅動頻率200~400 Hz之運動分析 38 3.4.1 驅動頻率為200 Hz下K.veneficum之運動分析 38 3.4.2 驅動頻率為400 Hz下K.veneficum之運動分析 41 3.5 流場擾動速度對K.veneficum之影響 44 3.5.1 K.veneficum對於2 Hz擾動流場速度之運動分析 44 3.5.2 K.veneficum對於4 Hz擾動流場速度之運動分析 46 3.5.3 K.veneficum對於20 Hz擾動流場速度之運動分析 49 3.5.4 K.veneficum對於40 Hz擾動流場速度之運動分析 52 3.5.5 K.veneficum對於200 Hz擾動流場速度之運動分析 55 3.5.6 K.veneficum對於400 Hz擾動流場速度之運動分析 58 第四章 結論與建議 62 4.1 結論 62 4.2 建議 64 參考文獻 65 | |
| dc.language.iso | zh-TW | |
| dc.subject | 擾流 | zh_TW |
| dc.subject | 卡羅藻 | zh_TW |
| dc.subject | 浮游生物運動 | zh_TW |
| dc.subject | 暫態行為響應 | zh_TW |
| dc.subject | 壓電制動 | zh_TW |
| dc.subject | 微流元件 | zh_TW |
| dc.subject | microfluidic device | en |
| dc.subject | dinoflagellates | en |
| dc.subject | flow disturbance | en |
| dc.subject | Karlodinium Veneficum | en |
| dc.subject | motility | en |
| dc.subject | transient behavioral response | en |
| dc.subject | piezoelectric actuation | en |
| dc.title | 浮游生物Karlodinium Veneficum (CCMP426)對於壓電驅動微擾流場之暫態運動行為反應 | zh_TW |
| dc.title | "Transient Change in motility of Karlodinium Veneficum, CCMP426 in response to intermittent flow disturbance generated by piezo-actuation" | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 謝志豪(Hsin-Tsai Liu),何東垣(Chih-Yang Tseng) | |
| dc.subject.keyword | 卡羅藻,浮游生物運動,暫態行為響應,壓電制動,微流元件,擾流, | zh_TW |
| dc.subject.keyword | Karlodinium Veneficum,motility,transient behavioral response,piezoelectric actuation,microfluidic device,flow disturbance,dinoflagellates, | en |
| dc.relation.page | 152 | |
| dc.identifier.doi | 10.6342/NTU202101931 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2021-08-13 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 機械工程學研究所 | zh_TW |
| 顯示於系所單位： | 機械工程學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| U0001-3007202114135300.pdf | 11.52 MB | Adobe PDF | 檢視/開啟 |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。