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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 白書禎(Su-Cheng Pai) | |
| dc.contributor.author | Yu-Ting Su | en |
| dc.contributor.author | 蘇渝婷 | zh_TW |
| dc.date.accessioned | 2022-11-23T09:14:36Z | - |
| dc.date.available | 2021-08-13 | |
| dc.date.available | 2022-11-23T09:14:36Z | - |
| dc.date.copyright | 2021-08-13 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-08-09 | |
| dc.identifier.citation | Beda, N., Nedospasov, A. (2005). A spectrophotometric assay for nitrate in an excess of nitrite. Nitric Oxide, 13(2), 93-97. Bianchi, T. S. (2007). Biogeochemistry of estuaries. Oxford University Press. Brewer, P. G., Riley, J. P. (1965). The automatic determination of nitrate in sea water. Deep Sea Research, 12, 765-722. Cecchini, S., Caputo, A. R. (2012). A Direct Spectrophotometric Assay for Evaluating Nitrate-Nitrogen in Intensive Aquaculture Systems. Israeli Journal of Aquaculture-Bamidgeh, 64, 698-705. Chen, C.-T. A., Wang, S.-L., Wang, B.-J., Pai, S.-C. (2001). Nutrient budgets for the South China Sea basin. Marine Chemistry, 75(4), 281-300. da Silva, G., Kennedy, E. M., Dlugogorski, B. Z. (2006). Ab initio procedure for aqueous-phase pKa calculation: the acidity of nitrous acid. J Phys Chem A, 110(39), 11371-11376. Doane, T. A., Horwath, W. R. (2003). Spectrophotometric determination of nitrate with a single reagent. Analytical Letters, 36(12), 2713-2722. Ellis, P. S., Shabani, A. M., Gentle, B. S., McKelvie, I. D. (2011). Field measurement of nitrate in marine and estuarine waters with a flow analysis system utilizing on-line zinc reduction. Talanta, 84(1), 98-103. Fang, T., Li, P., Lin, K., Chen, N., Jiang, Y., Chen, J., . . . Ma, J. (2019). Simultaneous underway analysis of nitrate and nitrite in estuarine and coastal waters using an automated integrated syringe-pump-based environmental-water analyzer. Anal Chim Acta, 1076, 100-109. Fang, T., Li, H., Bo, G., Lin, K., Yuan, D., Ma, J. (2021). On-site detection of nitrate plus nitrite in natural water samples using smartphone-based detection. Microchemical Journal, 165, 106117. Fox, J. B. (1979). Kinetics and Mechanisms of the Griess Reaction. Analytical Chemistry, 51(9), 1493-1502. García-Robledo, E., Corzo, A., Papaspyrou, S. (2014). A fast and direct spectrophotometric method for the sequential determination of nitrate and nitrite at low concentrations in small volumes. Marine Chemistry, 162, 30-36. Gong, G. C., Liu, K. K., Liu, C. T., Pai, S. C. (1992). The Chemical Hydrography of the South China Sea West of Luzon and a Comparison with the West Philippine Sea. Terrestrial, Atmospheric and Oceanic Sciences, 3, 587-602. Harvey, H. W. (1926). Nitrate in the sea. Jouranl of the Marine Biological Association, 14, 71-88. Hendrix, S. A., Braman, R. S. (1989 1995). Determination of Nitrite and Nitrate by Vanadium(III) Reduction with Chemiluminescence Detection. Methods, 7(1), 91-97. Kodamatani, H., Yamazaki, S., Saito, K., Tomiyasu, T., Komatsu, Y. (2009). Selective determination method for measurement of nitrite and nitrate in water samples using high-performance liquid chromatography with post-column photochemical reaction and chemiluminescence detection. Journal of Chromatography A, 1216(15), 3163-3167. Krockel, L., Schwotzer, G., Lehmann, H., Wieduwilt, T. (2011). Spectral optical monitoring of nitrate in inland and seawater with miniaturized optical components. Water Research, 45(3), 1423-1431. Libes, S. M. (2009). Introduction to Marine Biogeochemistry. Academic Press. Lin, K., Li, P., Ma, J., Yuan, D. (2019). An automatic reserve flow injection method using vanadium (III) reduction for simultaneous determination of nitrite and nitrate in estuarine and coastal waters. Talanta, 195, 613-618. Lin, K., Xu, J., Dong, X., Huo, Y., Yuan, D., Lin, H., Zhang, Y. (2020). An automated spectrophotometric method for the direct determination of nitrite and nitrate in seawater: Nitrite removal with sulfamic acid before nitrate reduction using the vanadium reduction method. Microchemical Journal, 158, 105272. Manea, F., Remes, A., Radovan, C., Pode, R., Picken, S., Schoonman, J. (2010). Simultaneous electrochemical determination of nitrate and nitrite in aqueous solution using Ag-doped zeolite-expanded graphite-epoxy electrode. Talanta, 83(1), 66-71. Merino, L. (2008). Development and Validation of a Method for Determination of Residual Nitrite/Nitrate in Foodstuffs and Water After Zinc Reduction. Food Analytical Methods, 2(3), 212-220. Miranda, K. M., Espey, M. G., Wink, D. A. (2001). A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide, 5(1), 62-71. Morris, A. W., Riley, J. P. (1963). The determination of nitrate in sea water. Analytica Chimica Acta, 29, 272-279. Mullin, J. B., Riley, J. P. (1955). The Spectrophotometric Determination of Nitrate in Natural Waters, with Particular Reference to Sea-Water. Analytica Chimica Acta, 12(5), 464-480. Pai, S. C., Yang, C. C., Riley, J. P. (1990). Formation Kinetics of the Pink Azo Dye in the Determination of Nitrite in Natural-Waters. Analytica Chimica Acta, 232(2), 345-349. Riley, J. P., Chester, R. (1971). Introduction to marine chemistry. Academic Press. Schnetger, B., Lehners, C. (2014). Determination of nitrate plus nitrite in small volume marine water samples using vanadium(III)chloride as a reduction agent. Marine Chemistry, 160, 91-98. Shariati-Rad, M., Irandoust, M., Haghighi, M. (2014). Chemometrics study of the kinetics of the Griess reaction. Journal of Chemometrics, 28(2), 93-99. Small, H., Stevens, T. S., Bauman, W. C. (1975). Novel Ion Exchange Chromatographic Method Using Conductimetric Detection. Analytical Chemistry, 47(11), 1801-1809. Tang, I. H., Sundari, R., Lintang, H. O., Yuliati, L. (2016). Detection of nitrite and nitrate ions in water by graphene oxide as a potential fluorescence sensor. IOP Conference Series: Materials Science and Engineering, 107, 012027. Turekian, K. K., Steven, J. H., Thorpe, S. A. (2010). Marine chemistry geochemistry: a derivative of the encyclopedia of ocean sciences. Academic Press. Valiente, N., Gomez-Alday, J. J., Jirsa, F. (2019). Spectrophotometric determination of nitrate in hypersaline waters after optimization based on the Box-Behnken design. Microchemical Journal, 145, 951-958. Vallance, C. (2017). An Introduction to Chemical Kinetics. Morgan Claypool Publishers. Wang, S., Lin, K., Chen, N., Yuan, D., Ma, J. (2016). Automated determination of nitrate plus nitrite in aqueous samples with flow injection analysis using vanadium (III) chloride as reductant. Talanta, 146, 744-748. Wood, E. D., Armstrong, F. A., Richards, F. A. (1967). Determination of Nitrate in Sea Water by Cadmium-Copper Reduction to Nitrite. Journal of the Marine Biological Association of the United Kingdom, 47(1), 23-31. Woollard, D. C., Indyk, H. E. (2014). Colorimetric determination of nitrate and nitrite in milk and milk powders - Use of vanadium (III) reduction. International Dairy Journal, 35(1), 88-94. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79869 | - |
| dc.description.abstract | "硝酸鹽(nitrate, NO₃⁻)為主要的氮營養鹽，是海洋浮游生物行光合作用合成有機物的原料，若海水中硝酸鹽不足將會導致藻類無法正常生長，進而影響基礎生產力乃至於整個海洋生物圈，是海洋化學的重要指標。傳統的測定法是利用鎘銅還原管(Cu-Cd column)將硝酸鹽還原成亞硝酸鹽後，再用格瑞氏反應(Griess reaction)測定，但鎘銅還原管的製備繁複且還原率不穩定。本研究嘗試另一種替代方式: 即採用三價釩當還原劑，並依粉紅偶氮呈色反應動力去尋找最佳的實驗條件，如試劑之添加順序、各種試劑濃度、加熱溫度、時間與冷卻步驟等。我們發現若將試劑預先混合會造成最後粉紅偶氮物的損失，但可以透過提高試劑比例將損失降到約6%。加熱溫度與時間控制也很重要，可避免粉紅偶氮物的快速褪色。經過各種條件調整比較，我們建議最佳的測定步驟是在樣水加入釩+格瑞氏的混合試劑後，置於50°C熱水浴中，加熱25-30分鐘，放入冷水浴中急速降溫，如此即可得到固定的最後吸光值。試劑濃度的條件為: SUL(sulfanilamide)試劑濃度約為30 mM，NED(N-1-naphtylethylenediamine)濃度約為0.5 mM，SUL/NED的莫爾比為60，VCl₃濃度為10 mM。在此條件下，硝酸鹽及亞硝酸鹽均會達到一個穩定且相同的莫爾吸光係數，約為50,000 M⁻¹cm⁻¹，且沒有顯著的鹽度效應。適用硝酸鹽濃度範圍0~50 μM，偵測極限為0.2 μM。本法操作簡便，結果精準，還原率為100%，非常適合於大量樣水的批次操作(batch operation)。" | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-23T09:14:36Z (GMT). No. of bitstreams: 1 U0001-3007202122203300.pdf: 6042844 bytes, checksum: bd6a18676333b541ee69687a2f3ffb0c (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 口試委員會審定書 I 致謝 II 中文摘要 III Abstract IV 目 錄 V 圖 目 錄 VIII 表 目 錄 IX 第一章 緒論 1 1.1 硝酸鹽在海洋中的分佈 1 1.2 硝酸鹽的測定原理及常用方法 1 1.3 鎘銅還原法 3 1.4 釩還原法測定硝酸鹽的文獻回顧 3 1.5 釩還原法的問題 5 1.6 本研究之目的與期待 6 第二章 粉紅偶氮化合物呈色反應動力學探討 10 2.1 概述 10 2.2 呈色曲線方程式與反應動力指標 10 2.3 莫爾吸光係數 11 2.4 酸度對粉紅偶氮物的影響 12 2.4.1 實驗目的 12 2.4.2 實驗步驟 12 2.4.3 實驗儀器 12 2.4.4 標準品與試劑配方 12 2.4.5 酸度控制 13 2.4.6 實驗結果與討論 14 2.5 混合試劑造成粉紅偶氮物的遺失 14 2.6 試劑添加次序與間隔時間對粉紅偶氮物的影響 14 2.6.1 實驗目的 14 2.6.2 實驗步驟 15 2.6.3 實驗儀器與試劑配方 15 2.6.4 實驗結果與討論 15 2.7 試劑濃度對粉紅偶氮物呈色之影響 16 2.7.1 實驗目的 16 2.7.2 實驗步驟 16 2.7.3 實驗儀器與試劑配方 16 2.7.4 試劑濃度的調整 17 2.7.5 實驗結果與討論 17 2.8 以形成常數探討混合試劑造成吸光值的減少 18 2.9 溫度對粉紅偶氮物的影響 20 2.9.1 實驗目的 20 2.9.2 實驗步驟 20 2.9.3 實驗儀器 20 2.9.4 標準品與試劑配方 21 2.9.5 實驗結果與討論 21 2.10 粉紅偶氮化合物呈色反應的綜合討論 22 第三章 釩還原法及格瑞氏反應之探討 31 3.1 概述 31 3.2 釩還原法能否避免將格瑞氏試劑預先混合? 31 3.2.1 實驗動機 31 3.2.2 實驗方法 31 3.2.3 實驗儀器、標準品與試劑配方 32 3.2.4 實驗結果與討論 32 3.3 釩+格瑞氏試劑測定硝酸鹽的呈色曲線 33 3.4 釩濃度對試劑空白值與亞硝酸鹽呈色之影響 33 3.5 溫度對釩還原格瑞氏法的影響 34 3.6 釩濃度、反應溫度、反應時間與鹽度效應對釩還原法的影響 34 3.6.1 實驗目的 34 3.6.2 實驗方法 35 3.6.3 實驗儀器、標準品與試劑配方 35 3.6.4 實驗結果與討論 36 3.7 控制溫度以降低粉紅偶氮物的褪色率 37 3.8 釩還原格瑞氏法的最佳條件及線性範圍 38 3.9 建議方法 39 3.9.1 測定亞硝酸鹽的建議方法(若濃度> 10 μM需稀釋後再進行測定) 40 3.9.2 測定硝酸鹽的建議方法(若濃度> 50 μM需稀釋後再進行測定) 40 3.10 釩還原法與傳統方法的比較 41 第四章 結論 51 參考文獻 52 附錄-刊登在ES T WATER期刊之論文 57 | |
| dc.language.iso | zh-TW | |
| dc.subject | 反應動力學 | zh_TW |
| dc.subject | 硝酸鹽 | zh_TW |
| dc.subject | 亞硝酸鹽 | zh_TW |
| dc.subject | 釩還原法 | zh_TW |
| dc.subject | 粉紅偶氮物 | zh_TW |
| dc.subject | Reaction kinetics | en |
| dc.subject | Pink azo dye | en |
| dc.subject | Vanadium reduction | en |
| dc.subject | Nitrite | en |
| dc.subject | Nitrate | en |
| dc.title | 以釩還原及格瑞氏反應測定海水中硝酸鹽之硏究：解析反應動力機制並設立最佳化條件 | zh_TW |
| dc.title | Determination of nitrate in seawater by vanadium reduction and Griess assay: reassessment and optimization | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳鎮東(Hsin-Tsai Liu),龔國慶(Chih-Yang Tseng),洪慶章,何東垣 | |
| dc.subject.keyword | 硝酸鹽,亞硝酸鹽,釩還原法,粉紅偶氮物,反應動力學, | zh_TW |
| dc.subject.keyword | Nitrate,Nitrite,Vanadium reduction,Pink azo dye,Reaction kinetics, | en |
| dc.relation.page | 66 | |
| dc.identifier.doi | 10.6342/NTU202101954 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2021-08-10 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 海洋研究所 | zh_TW |
| 顯示於系所單位： | 海洋研究所 | |
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