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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 王珮玲(Pei-Ling Wang) | |
| dc.contributor.author | Pei-En Chen | en |
| dc.contributor.author | 陳培恩 | zh_TW |
| dc.date.accessioned | 2022-11-23T09:08:46Z | - |
| dc.date.available | 2023-08-24 | |
| dc.date.available | 2022-11-23T09:08:46Z | - |
| dc.date.copyright | 2021-09-02 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-08-24 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79721 | - |
| dc.description.abstract | 二氧化碳是重要的溫室氣體，在調控大氣二氧化碳的諸多機制中，生物作用和岩石風化作用均扮演關鍵的角色。河川被視為匯集風化產物並輸送到海洋的通道，然而過去對於河川系統的研究大都聚焦於大河系統所記錄的風化作用形式、速率與影響因子，往往忽略來自河川內部進行的生地化作用，亦可能對大氣二氧化碳的調節與貢獻。近來有研究顯示高山河川對於二氧化碳收支的影響超出預期，特別是於造山帶的小河系統中，河水滯留時間短，並有大量溶質與沉積物輸送至河道，提供豐沛的岩石源礦物質與有機質，作為河域自營作用 (Autotrophy) 和異營作用 (Heterotrophy) 可能的基質，分別消耗和釋出二氧化碳，過去卻鮮有研究探討此兩種代謝形式的實際速率與控制因素，及其對於流域二氧化碳收支的影響。 本研究旨在研究台灣東部卑南溪流域生物性碳轉過程在時空上的變化，並討論其對於二氧化碳收支的影響。卑南溪流域有全台灣最高的沉積物輸出通量與高化學風化速率。本研究收集不同季節上至下游的河水樣本，添加標定 ¹³C 同位素之無機碳與有機碳源進行培養實驗，量測自營與異營作用速率，並進行地化和分子生物分析，釐清相關控制因子和參與作用的微生物族群組成。結果顯示，利用穩定碳同位素標定培養實驗追蹤河水中生物性碳的轉換，可確實偵測到生物對於無機碳和有機碳的合成作用，生物性碳轉換速率於卑南溪出海口最高、普遍濕季明顯高於乾季，具有季節性與地域性的差異，探討生物性碳的轉換速率和其他環境因子之間的關係，可發現河水溫度與氮、磷營養鹽為重要的影響因子。綜觀整個生物作用轉換碳的過程，全年皆為異營潛在代謝產生二氧化碳速率高於自營生物合成消耗的速率，推測卑南溪流域屬於異養河川系統，對大氣二氧化碳有淨輸出的潛力。分子生物方面，根據 16S rRNA 基因分析結果發現，流域上至下游有不同的微生物族群組成，亦有季節性的差異，可能在自營與異營作用中扮演各式角色。進一步估算卑南溪流域可消耗的二氧化碳通量約介於 2.4 × 10⁷ 至 1.1 × 10⁸ mole/year 間，而可產生的二氧化碳通量約介於2.1 × 10⁹ 至 1.8 × 10¹⁰ mole/year 間，顯示卑南溪流域之生物性碳轉換作用對於大氣二氧化碳具有一定程度的影響力。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-23T09:08:46Z (GMT). No. of bitstreams: 1 U0001-2208202115500300.pdf: 11965422 bytes, checksum: c27e150b32ddf2938478f8c35f0bf7c8 (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | "誌謝 i 摘要 ii Abstract iii 目錄 v 圖目錄 viii 表目錄 ix 第一章、 緒論 1 1.1. 河川在全球碳循環中的角色 1 1.2. 陸域環境中調控大氣二氧化碳的機制 3 1.3. 河川中進行之生物性碳轉換 4 1.4. 造山帶河川系統之重要性 7 1.5. 研究動機與目的 9 第二章、 研究材料與方法 11 2.1. 研究區域概況 11 2.2. 採樣地點與方法 12 2.3. 培養實驗設置 15 2.4. 地球化學分析 16 2.4.1. 顆粒態有機碳之穩定碳同位素與濃度分析 16 2.4.2. 溶解態無機碳濃度分析 18 2.4.3. 溶解態無機碳之穩定同位素分析 18 2.4.4. 葉綠素甲濃度分析 19 2.4.5. 營養鹽濃度分析 20 2.4.5.1. 亞硝酸鹽與硝酸鹽濃度分析 20 2.4.5.2. 氨氮濃度分析 20 2.4.5.3. 磷酸鹽濃度分析 21 2.4.6. 硫酸鹽濃度分析 21 2.5. 碳轉換速率計算 22 2.6. 微生物族群結構分析 23 2.6.1. 樣本 DNA 萃取與備製 23 2.6.2. 定序分析與資料處理 24 2.7. 微生物定量分析 25 2.7.1. 即時定量聚合酶連鎖反應 (Real-Time PCR, qPCR) 25 2.7.2. qPCR 標準品製備 27 2.7.3. 水中微生物總量計算 28 2.8. 統計分析方法 28 2.8.1. 相關性分析 28 2.8.2. 群集分析 29 第三章、 研究結果 30 3.1 地球化學分析 30 3.1.1. 馬里蘭溪 (MLL01) 31 3.1.2. 新武呂溪 (CWL01) 31 3.1.3. 大崙溪 (DL) 32 3.1.4. 鹿野溪 (LY04) 33 3.1.5. 卑南溪出海口 (BNE) 34 3.2. 碳轉換速率 36 3.2.1. 生物自營合成速率 36 3.2.2. 生物異營代謝速率 37 3.2.3. 生物性碳轉換 38 3.3. 微生物族群結構分析 42 3.3.1. 初始環境之微生物族群組成 42 3.3.2. 初始環境之微生物群集分析 43 3.3.3. 培養前後之微生物族群組成 44 3.3.4. 培養前後之微生物群集分析 48 3.4. 微生物族群豐度 54 3.4.1. 初始環境之微生物豐度 54 3.4.2. 培養樣本之微生物豐度 56 第四章、 討論 61 4.1. 生物性碳轉換速率之時空變化 61 4.2. 環境因子對碳轉換速率之影響 62 4.2.1. 生物自營合成速率 62 4.2.2. 生物異營潛在代謝速率 63 4.3. 微生物族群結構與豐度變化 65 4.3.1. 環境微生物組成特徵 65 4.3.2. 培養前後微生物菌群結構變化 66 4.3.3. 微生物量與環境因子之相關性 70 4.4. 卑南溪流域生物性碳轉換之貢獻 71 4.5. 卑南溪流域生物性碳轉換速率與其他河川、水域系統比較 73 第五章、 結論 86 參考文獻 87 附錄 100" | |
| dc.language.iso | zh-TW | |
| dc.subject | ¹³C同位素標定 | zh_TW |
| dc.subject | 自營作用 | zh_TW |
| dc.subject | 異營作用 | zh_TW |
| dc.subject | ¹³C isotope labeling | en |
| dc.subject | autotrophy | en |
| dc.subject | heterotrophy | en |
| dc.title | 卑南溪流域河水中自營與異營作用的時空變化 | zh_TW |
| dc.title | Spatiotemporal variations of riverine autotrophy and heterotrophy in the Beinan river system | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林立虹(Hsin-Tsai Liu),林玉詩(Chih-Yang Tseng),塗子萱 | |
| dc.subject.keyword | 自營作用,異營作用,¹³C同位素標定, | zh_TW |
| dc.subject.keyword | autotrophy,heterotrophy,¹³C isotope labeling, | en |
| dc.relation.page | 104 | |
| dc.identifier.doi | 10.6342/NTU202102587 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2021-08-25 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 海洋研究所 | zh_TW |
| dc.date.embargo-lift | 2023-08-24 | - |
| 顯示於系所單位： | 海洋研究所 | |
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