請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/7968完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李士傑 | |
| dc.contributor.author | Shuo-Peng Lin | en |
| dc.contributor.author | 林碩芃 | zh_TW |
| dc.date.accessioned | 2021-05-19T18:00:45Z | - |
| dc.date.available | 2026-06-07 | |
| dc.date.available | 2021-05-19T18:00:45Z | - |
| dc.date.copyright | 2016-07-06 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-06-07 | |
| dc.identifier.citation | Amat, J. A., K. L. Fields and U. K. Schubart (1990). 'Stage-specific expression of phosphoprotein p19 during spermatogenesis in the rat.' Mol Reprod Dev 26(4): 383-390.
Amayed, P., M. F. Carlier and D. Pantaloni (2000). 'Stathmin slows down guanosine diphosphate dissociation from tubulin in a phosphorylation-controlled fashion.' Biochemistry 39(40): 12295-12302. Anderson, D. J. and R. Axel (1985). 'Molecular probes for the development and plasticity of neural crest derivatives.' Cell 42(2): 649-662. Baldassarre, G., B. Belletti, M. S. Nicoloso, M. Schiappacassi, A. Vecchione, P. Spessotto, A. Morrione, V. Canzonieri and A. Colombatti (2005). 'p27(Kip1)-stathmin interaction influences sarcoma cell migration and invasion.' Cancer Cell 7(1): 51-63. Beilharz, E. J., E. Zhukovsky, A. A. Lanahan, P. F. Worley, K. Nikolich and L. J. Goodman (1998). 'Neuronal activity induction of the stathmin-like gene RB3 in the rat hippocampus: possible role in neuronal plasticity.' J Neurosci 18(23): 9780-9789. Belletti, B. and G. Baldassarre (2011). 'Stathmin: a protein with many tasks. New biomarker and potential target in cancer.' Expert Opin Ther Targets 15(11): 1249-1266. Beretta, L., T. Dobransky and A. Sobel (1993). 'Multiple phosphorylation of stathmin. Identification of four sites phosphorylated in intact cells and in vitro by cyclic AMP-dependent protein kinase and p34cdc2.' J Biol Chem 268(27): 20076-20084. Bieche, I., A. Maucuer, I. Laurendeau, S. Lachkar, A. J. Spano, A. Frankfurter, P. Levy, V. Manceau, A. Sobel, M. Vidaud and P. A. Curmi (2003). 'Expression of stathmin family genes in human tissues: non-neural-restricted expression for SCLIP.' Genomics 81(4): 400-410. Boekhoorn, K., V. van Dis, E. Goedknegt, A. Sobel, P. J. Lucassen and C. C. Hoogenraad (2014). 'The microtubule destabilizing protein stathmin controls the transition from dividing neuronal precursors to postmitotic neurons during adult hippocampal neurogenesis.' Dev Neurobiol 74(12): 1226-1242. Bohm, M. R., S. Mertsch, S. Konig, T. Spieker and S. Thanos (2013). 'Macula-less rat and macula-bearing monkey retinas exhibit common lifelong proteomic changes.' Neurobiol Aging 34(11): 2659-2675. Brannstrom, K., B. Segerman and M. Gullberg (2003). 'Molecular dissection of GTP exchange and hydrolysis within the ternary complex of tubulin heterodimers and Op18/stathmin family members.' J Biol Chem 278(19): 16651-16657. Burzynski, G. M., J. M. Delalande and I. Shepherd (2009). 'Characterization of spatial and temporal expression pattern of SCG10 during zebrafish development.' Gene Expr Patterns 9(4): 231-237. Charbaut, E., S. Chauvin, H. Enslen, S. Zamaroczy and A. Sobel (2005). 'Two separate motifs cooperate to target stathmin-related proteins to the Golgi complex.' J Cell Sci 118(Pt 10): 2313-2323. Charbaut, E., P. A. Curmi, S. Ozon, S. Lachkar, V. Redeker and A. Sobel (2001). 'Stathmin family proteins display specific molecular and tubulin binding properties.' J Biol Chem 276(19): 16146-16154. Chauvin, S., F. E. Poulain, S. Ozon and A. Sobel (2008). 'Palmitoylation of stathmin family proteins domain A controls Golgi versus mitochondrial subcellular targeting.' Biol Cell 100(10): 577-589. Chauvin, S. and A. Sobel (2015). 'Neuronal stathmins: A family of phosphoproteins cooperating for neuronal development, plasticity and regeneration.' Prog Neurobiol 126: 1-18. Cheng, H. W., T. Jiang, N. Mori and T. H. McNeill (1997). 'Upregulation of stathmin (p19) gene expression in adult rat brain during injury-induced synapse formation.' Neuroreport 8(17): 3691-3695. Curmi, P. A., O. Gavet, E. Charbaut, S. Ozon, S. Lachkar-Colmerauer, V. Manceau, S. Siavoshian, A. Maucuer and A. Sobel (1999). 'Stathmin and its phosphoprotein family: general properties, biochemical and functional interaction with tubulin.' Cell Struct Funct 24(5): 345-357. Del Rio, J. A., C. Gonzalez-Billault, J. M. Urena, E. M. Jimenez, M. J. Barallobre, M. Pascual, L. Pujadas, S. Simo, A. La Torre, F. Wandosell, J. Avila and E. Soriano (2004). 'MAP1B is required for Netrin 1 signaling in neuronal migration and axonal guidance.' Curr Biol 14(10): 840-850. Di Paolo, G., B. Antonsson, D. Kassel, B. M. Riederer and G. Grenningloh (1997). 'Phosphorylation regulates the microtubule-destabilizing activity of stathmin and its interaction with tubulin.' FEBS Lett 416(2): 149-152. Di Paolo, G., R. Lutjens, A. Osen-Sand, A. Sobel, S. Catsicas and G. Grenningloh (1997). 'Differential distribution of stathmin and SCG10 in developing neurons in culture.' J Neurosci Res 50(6): 1000-1009. Di Paolo, G., R. Lutjens, V. Pellier, S. A. Stimpson, M. H. Beuchat, S. Catsicas and G. Grenningloh (1997). 'Targeting of SCG10 to the area of the Golgi complex is mediated by its NH2-terminal region.' J Biol Chem 272(8): 5175-5182. Doye, V., M. C. Boutterin and A. Sobel (1990). 'Phosphorylation of stathmin and other proteins related to nerve growth factor-induced regulation of PC12 cells.' J Biol Chem 265(20): 11650-11655. Grenningloh, G., S. Soehrman, P. Bondallaz, E. Ruchti and H. Cadas (2004). 'Role of the microtubule destabilizing proteins SCG10 and stathmin in neuronal growth.' J Neurobiol 58(1): 60-69. Gupta, K. K., C. Li, A. Duan, E. O. Alberico, O. V. Kim, M. S. Alber and H. V. Goodson (2013). 'Mechanism for the catastrophe-promoting activity of the microtubule destabilizer Op18/stathmin.' Proc Natl Acad Sci U S A 110(51): 20449-20454. Hayashi, K., Y. Pan, H. Shu, T. Ohshima, J. W. Kansy, C. L. White, 3rd, C. A. Tamminga, A. Sobel, P. A. Curmi, K. Mikoshiba and J. A. Bibb (2006). 'Phosphorylation of the tubulin-binding protein, stathmin, by Cdk5 and MAP kinases in the brain.' J Neurochem 99(1): 237-250. Himi, T., T. Okazaki, H. Wang, T. H. McNeill and N. Mori (1994). 'Differential localization of SCG10 and p19/stathmin messenger RNAs in adult rat brain indicates distinct roles for these growth-associated proteins.' Neuroscience 60(4): 907-926. Honnappa, S., W. Jahnke, J. Seelig and M. O. Steinmetz (2006). 'Control of intrinsically disordered stathmin by multisite phosphorylation.' J Biol Chem 281(23): 16078-16083. Howard, J. and A. A. Hyman (2009). 'Growth, fluctuation and switching at microtubule plus ends.' Nat Rev Mol Cell Biol 10(8): 569-574. Jin, L. W., E. Masliah, D. Iimoto, R. Deteresa, M. Mallory, M. Sundsmo, N. Mori, A. Sobel and T. Saitoh (1996). 'Neurofibrillary tangle-associated alteration of stathmin in Alzheimer's disease.' Neurobiol Aging 17(3): 331-341. Koppel, J., M. C. Boutterin, V. Doye, H. Peyro-Saint-Paul and A. Sobel (1990). 'Developmental tissue expression and phylogenetic conservation of stathmin, a phosphoprotein associated with cell regulations.' J Biol Chem 265(7): 3703-3707. Koppel, J., P. Rehak, V. Baran, J. Vesela, D. Hlinka, V. Manceau and A. Sobel (1999). 'Cellular and subcellular localization of stathmin during oocyte and preimplantation embryo development.' Mol Reprod Dev 53(3): 306-317. Lachkar, S., M. Lebois, M. O. Steinmetz, A. Guichet, N. Lal, P. A. Curmi, A. Sobel and S. Ozon (2010). 'Drosophila stathmins bind tubulin heterodimers with high and variable stoichiometries.' J Biol Chem 285(15): 11667-11680. Larsson, N., B. Segerman, B. Howell, K. Fridell, L. Cassimeris and M. Gullberg (1999). 'Op18/stathmin mediates multiple region-specific tubulin and microtubule-regulating activities.' J Cell Biol 146(6): 1289-1302. Ledda, F., G. Paratcha and C. F. Ibanez (2002). 'Target-derived GFRalpha1 as an attractive guidance signal for developing sensory and sympathetic axons via activation of Cdk5.' Neuron 36(3): 387-401. Li, D. K., S. Tisdale, F. Lotti and L. Pellizzoni (2014). 'SMN control of RNP assembly: from post-transcriptional gene regulation to motor neuron disease.' Semin Cell Dev Biol 32: 22-29. Li, S., M. Yin, S. Liu, Y. Chen, Y. Yin, T. Liu and J. Zhou (2010). 'Expression of ventral diencephalon-enriched genes in zebrafish.' Dev Dyn 239(12): 3368-3379. Liedtke, W., E. E. Leman, R. E. Fyffe, C. S. Raine and U. K. Schubart (2002). 'Stathmin-deficient mice develop an age-dependent axonopathy of the central and peripheral nervous systems.' Am J Pathol 160(2): 469-480. Liu, A., C. Stadelmann, M. Moscarello, W. Bruck, A. Sobel, F. G. Mastronardi and P. Casaccia-Bonnefil (2005). 'Expression of stathmin, a developmentally controlled cytoskeleton-regulating molecule, in demyelinating disorders.' J Neurosci 25(3): 737-747. Lutjens, R., M. Igarashi, V. Pellier, H. Blasey, G. Di Paolo, E. Ruchti, C. Pfulg, J. K. Staple, S. Catsicas and G. Grenningloh (2000). 'Localization and targeting of SCG10 to the trans-Golgi apparatus and growth cone vesicles.' Eur J Neurosci 12(7): 2224-2234. Manceau, V., O. Gavet, P. Curmi and A. Sobel (1999). 'Stathmin interaction with HSC70 family proteins.' Electrophoresis 20(2): 409-417. Manna, T., D. A. Thrower, S. Honnappa, M. O. Steinmetz and L. Wilson (2009). 'Regulation of microtubule dynamic instability in vitro by differentially phosphorylated stathmin.' J Biol Chem 284(23): 15640-15649. Maucuer, A., J. Moreau, M. Mechali and A. Sobel (1993). 'Stathmin gene family: phylogenetic conservation and developmental regulation in Xenopus.' J Biol Chem 268(22): 16420-16429. Mistry, S. J. and G. F. Atweh (2006). 'Therapeutic interactions between stathmin inhibition and chemotherapeutic agents in prostate cancer.' Mol Cancer Ther 5(12): 3248-3257. Mistry, S. J., A. Bank and G. F. Atweh (2005). 'Targeting stathmin in prostate cancer.' Mol Cancer Ther 4(12): 1821-1829. Morii, H., Y. Shiraishi-Yamaguchi and N. Mori (2006). 'SCG10, a microtubule destabilizing factor, stimulates the neurite outgrowth by modulating microtubule dynamics in rat hippocampal primary cultured neurons.' J Neurobiol 66(10): 1101-1114. Morii, H., T. Yamada, I. Nakano, J. M. Coulson and N. Mori (2006). 'Site-specific phosphorylation of SCG10 in neuronal plasticity: role of Ser73 phosphorylation by N-methyl D-aspartic acid receptor activation in rat hippocampus.' Neurosci Lett 396(3): 241-246. Nakao, C., T. J. Itoh, H. Hotani and N. Mori (2004). 'Modulation of the stathmin-like microtubule destabilizing activity of RB3, a neuron-specific member of the SCG10 family, by its N-terminal domain.' J Biol Chem 279(22): 23014-23021. Ng, D. C., T. T. Zhao, Y. Y. Yeap, K. R. Ngoei and M. A. Bogoyevitch (2010). 'c-Jun N-terminal kinase phosphorylation of stathmin confers protection against cellular stress.' J Biol Chem 285(37): 29001-29013. Okazaki, T., H. Wang, E. Masliah, M. Cao, S. A. Johnson, M. Sundsmo, T. Saitoh and N. Mori (1995). 'SCG10, a neuron-specific growth-associated protein in Alzheimer's disease.' Neurobiol Aging 16(6): 883-894. Okazaki, T., B. N. Yoshida, K. B. Avraham, H. Wang, C. W. Wuenschell, N. A. Jenkins, N. G. Copeland, D. J. Anderson and N. Mori (1993). 'Molecular diversity of the SCG10/stathmin gene family in the mouse.' Genomics 18(2): 360-373. Ozon, S., T. Byk and A. Sobel (1998). 'SCLIP: a novel SCG10-like protein of the stathmin family expressed in the nervous system.' J Neurochem 70(6): 2386-2396. Ozon, S., A. Guichet, O. Gavet, S. Roth and A. Sobel (2002). 'Drosophila stathmin: a microtubule-destabilizing factor involved in nervous system formation.' Mol Biol Cell 13(2): 698-710. Pedraza, N., R. Ortiz, A. Cornado, A. Llobet, M. Aldea and C. Gallego (2014). 'KIS, a kinase associated with microtubule regulators, enhances translation of AMPA receptors and stimulates dendritic spine remodeling.' J Neurosci 34(42): 13988-13997. Poulain, F. E., S. Chauvin, R. Wehrle, M. Desclaux, J. Mallet, G. Vodjdani, I. Dusart and A. Sobel (2008). 'SCLIP is crucial for the formation and development of the Purkinje cell dendritic arbor.' J Neurosci 28(29): 7387-7398. Poulain, F. E. and A. Sobel (2007). 'The 'SCG10-LIke Protein' SCLIP is a novel regulator of axonal branching in hippocampal neurons, unlike SCG10.' Mol Cell Neurosci 34(2): 137-146. Poulain, F. E. and A. Sobel (2010). 'The microtubule network and neuronal morphogenesis: Dynamic and coordinated orchestration through multiple players.' Mol Cell Neurosci 43(1): 15-32. Ringhoff, D. N. and L. Cassimeris (2009). 'Gene expression profiles in mouse embryo fibroblasts lacking stathmin, a microtubule regulatory protein, reveal changes in the expression of genes contributing to cell motility.' BMC Genomics 10: 343. Rubin, C. I. and G. F. Atweh (2004). 'The role of stathmin in the regulation of the cell cycle.' J Cell Biochem 93(2): 242-250. Sone, K., M. Tsuda and N. Mori (2011). 'Position-dependent effect of a neural-restrictive silencer-like element present in the promoter downstream of the SCG10-like protein gene.' J Biochem 150(4): 451-460. Su, D., S. M. Smith, M. Preti, P. Schwartz, T. J. Rutherford, G. Menato, S. Danese, S. Ma, H. Yu and D. Katsaros (2009). 'Stathmin and tubulin expression and survival of ovarian cancer patients receiving platinum treatment with and without paclitaxel.' Cancer 115(11): 2453-2463. Trovik, J., E. Wik, I. M. Stefansson, J. Marcickiewicz, S. Tingulstad, A. C. Staff, T. S. Njolstad, G. MoMaTec Study, I. Vandenput, F. Amant, L. A. Akslen and H. B. Salvesen (2011). 'Stathmin overexpression identifies high-risk patients and lymph node metastasis in endometrial cancer.' Clin Cancer Res 17(10): 3368-3377. Vandenput, I., J. Trovik, K. Leunen, E. Wik, I. Stefansson, L. Akslen, P. Moerman, I. Vergote, H. Salvesen and F. Amant (2011). 'Evolution in endometrial cancer: evidence from an immunohistochemical study.' Int J Gynecol Cancer 21(2): 316-322. Verma, N. K., J. Dourlat, A. M. Davies, A. Long, W. Q. Liu, C. Garbay, D. Kelleher and Y. Volkov (2009). 'STAT3-stathmin interactions control microtubule dynamics in migrating T-cells.' J Biol Chem 284(18): 12349-12362. Wang, C., A. Cormier, B. Gigant and M. Knossow (2007). 'Insight into the GTPase activity of tubulin from complexes with stathmin-like domains.' Biochemistry 46(37): 10595-10602. Wang, J., C. Shan, W. Cao, C. Zhang, J. Teng and J. Chen (2013). 'SCG10 promotes non-amyloidogenic processing of amyloid precursor protein by facilitating its trafficking to the cell surface.' Hum Mol Genet 22(24): 4888-4900. Watabe-Uchida, M., K. A. John, J. A. Janas, S. E. Newey and L. Van Aelst (2006). 'The Rac activator DOCK7 regulates neuronal polarity through local phosphorylation of stathmin/Op18.' Neuron 51(6): 727-739. Wen, H. L., Y. T. Lin, C. H. Ting, S. Lin-Chao, H. Li and H. M. Hsieh-Li (2010). 'Stathmin, a microtubule-destabilizing protein, is dysregulated in spinal muscular atrophy.' Hum Mol Genet 19(9): 1766-1778. Westerlund, N., J. Zdrojewska, A. Padzik, E. Komulainen, B. Bjorkblom, E. Rannikko, T. Tararuk, C. Garcia-Frigola, J. Sandholm, L. Nguyen, T. Kallunki, M. J. Courtney and E. T. Coffey (2011). 'Phosphorylation of SCG10/stathmin-2 determines multipolar stage exit and neuronal migration rate.' Nat Neurosci 14(3): 305-313. Wojcechowskyj, J. A., J. Y. Lee, S. H. Seeholzer and R. W. Doms (2011). 'Quantitative phosphoproteomics of CXCL12 (SDF-1) signaling.' PLoS One 6(9): e24918. Yang, H., Y. Zhou, J. Gu, S. Xie, Y. Xu, G. Zhu, L. Wang, J. Huang, H. Ma and J. Yao (2013). 'Deep mRNA sequencing analysis to capture the transcriptome landscape of zebrafish embryos and larvae.' PLoS One 8(5): e64058. Yip, Y. Y., Y. Y. Yeap, M. A. Bogoyevitch and D. C. Ng (2014). 'Differences in c-Jun N-terminal kinase recognition and phosphorylation of closely related stathmin-family members.' Biochem Biophys Res Commun 446(1): 248-254. Zhang, B., C. Xuan, Y. Ji, W. Zhang and D. Wang (2015). 'Zebrafish xenotransplantation as a tool for in vivo cancer study.' Fam Cancer. Zhang, H. Z., Y. Wang, P. Gao, F. Lin, L. Liu, B. Yu, J. H. Ren, H. Zhao and R. Wang (2006). 'Silencing stathmin gene expression by survivin promoter-driven siRNA vector to reverse malignant phenotype of tumor cells.' Cancer Biol Ther 5(11): 1457-1461 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/7968 | - |
| dc.description.abstract | 在真核生物，微管在調節細胞生理訊息傳遞的過程中扮演很重要的角色，它能夠穩定細胞骨架以及影響神經發育。有許多不同的微管蛋白能夠調節微管的聚合，其中包含stathmin。stathmin蛋白是一種磷酸蛋白，它可調節微管的聚合，stathmin能夠和兩分子的α-和β-微管蛋白形成的二聚體結合，形成一個四聚體，因此stathmin能夠干擾微管的聚合化和去聚合化。藉由調節微管的聚合和去聚合，stathmin參與了許多不同的細胞訊息傳遞的過程，包括神經形成，細胞的分化和增生。在哺乳類的研究中指出stmn1廣泛的表現在細胞中，而stmn2，stmn3以及stmn4則主要表現在神經系統，在先前我們實驗室的研究，發現在斑馬魚中的一個stathmin蛋白，stathmin 2，在腦部發育中，扮演一個很重要的角色。目前已知斑馬魚中有7個不同的stathmin基因，為了瞭解stathmin在斑馬魚發育中扮演的角色，我選殖出斑馬魚stathmin蛋白的基因stmn 1a，stmn 2b和stmn 4l，發現它們和其他脊椎動物的stathmin蛋白有很高的相似度，並利用全標本包埋的原位雜交技術觀察斑馬魚stathmin蛋白在不同時間點，不同的斑馬魚stathmin蛋白的表現情形。在此研究中我觀察到，stmn 1a和stmn 2b從16小時就會表現在脊髓和三叉神經，但是stmn 4l要從18小時才會開始表現，而stmn 2b和stmn 4l主要會表現在中樞神經系統。此外，stmn 1a也會表現在眼睛，鰓弓以及胚胎的尾端。從24小時後，stmn 1a，stmn 2b和stmn 4l都會表現在下視丘，頭蓋，間腦以及端腦，在48小時後，stmn 2b和stmn 4l仍然會表現在中樞神經系統，不過stmn 1a則沒有表現在後腦。stathmin在中樞神經系統發育中的表現情形，顯示stathmin在斑馬魚中樞神經系統發育上，可能扮演一個很重要的角色。 | zh_TW |
| dc.description.abstract | Microtubules are well known to mediate many physiological processes in eukaryotic cell such as stable cell structure and axonal outgrowth. Microtubules can be regulated by different microtubule binding proteins to mediated their assembly and disassembly. Stathmin family proteins are phosphoprotein that regulates microtubules disassembly. Stathmin interacts with microtubules by binding to two moles of alpha and beta tubulin dimer to form a tetramer. Stathmins also interfere with microtubule polymerization by direct binding to microtubules. Via tight regulation of microtubule polymerization and depolymerization, stathmins are involved in different cellular processes, including neurogenesis, cell proliferation, differentiation and activities. In mammals, stmn 1 is ubiquitous expressed while other stathmin members, stmn 2, stmn 3, stmn 4 are mainly expressed in the nervous system. Previously, we have demonstrated that stmn 2, one of the zebrafish stathmin proteins, plays a vital role in zebrafish brain development. There are seven different stathmin genes in zebrafish. To understand the role of stathmin in zebrafish, I have cloned stmn 1a, stmn 2b and stmn 4l and demonstrated that they shared high identity with other vertebrate stathmin homologues. I analyzsed their temporal expression patterns using embryos at different stages by whole-mount in situ hybridization. I observed that stmn 1a and stmn 2b were expressed in spinal cord and trigeminal ganglia from 16 h post fertilization (hpf) but stmn 4l appeared from 18 hpf. stmn2b and stmn4l were mainly expressed in the central nervous system (CNS). Furthermore, the stmn1a transcripts were found in eyes, pharyngeal arches and tail bud. stmn 1a, stmn 2b and stmn4l were all expressed in hypothalamus, tegmentum, telencephalon and diencephalon after 24 hpf. After 48 hpf, stmn2b and stmn4l were still expressed in the CNS while stmn1a did not expressed in hindbrain. The dynamic expression of stathmin family genes in the developing central nervous system suggests a pivotal roles of those genes in regulating CNS development in zebrafish. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-19T18:00:45Z (GMT). No. of bitstreams: 1 ntu-105-R02b21030-1.pdf: 2053854 bytes, checksum: e5486298461285b2b75213e5ffafa01b (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 誌謝……………………………………………………………………………….I
中文摘要…………………………………………………………………………II Abstract………………………………………………………………………………..IV Introduction…………………………………………………………………………... 3 Stathmin family are highly conserved in vertebrates……………………………….. 3 Stathmins are involved in neuronal development and differentiation……………… 4 Mechanisms of stathmin-mediated microtubule disassembly……………………… 8 Stathmin-related diseases………………………………………………………….. 11 In vivo function of Stathmins……………………………………………………… 12 Materials and methods………………………………………………………………. 14 Zebrafish maintenance and embryo yielding………………………………………. 14 In silica cloning and sequence analysis…………………………………………….. 14 Whole mount in situ hybridization and RNA probe prepared……………………... 15 RT-PCR……………………………………………………………………………. 15 Photography of in situ hybridization………………………………………………. 16 Results………………………………………………………………………………. 17 Sequence analysis and the phylogenetic tree of zebrafish stathmin family proteins.17 Expression pattern analysis of stathmin by RT-PCR……………………………… 18 Expression pattern analysis of stathmin by whole-mount in situ hybridization…… 19 Discussion………………………………………………………………………….... 22 References…………………………………………………………………………… 29 Table…………………………………………………………………………….…… 41 Table 1 Primer pairs used in this study...................................................................... 41 Figures………………………………………………………………………………. 42 Figure. 1 Zebrafish stathmin sequence analysis of stmn1a, stmn2b and stmn4l…... 42 Figure. 2 Comparison of all zebrafish stathmin family genes……………………… 43 Figure. 3 The phylogenetic analysis between major model animal and zebrafish stathmin family…………………………………………………………………………………. 44 Figure. 4 Expression pattern of stathmin family genes during development by RT-PCR analysis………………………………………………………………………………. 45 Figure. 5 Spatial expression pattern of stathmin1a during segmentation stages…… 46 Figure. 6 Spatial expression pattern of stathmin1a early larval stages……………... 47 Figure. 7 Spatial expression pattern of stathmin2b during segmentation stages…… 48 Figure. 8 Spatial expression pattern of stathmin2b early larval stages……………... 49 Figure. 9 Spatial expression pattern of stathmin4l during segmentation stages……. 50 Figure. 10 Spatial expression pattern of stathmin4l during early larval stages…….. 51 | |
| dc.language.iso | en | |
| dc.title | 分析斑馬魚不同stathmin基因在胚胎中的表現 | zh_TW |
| dc.title | Expression analysis of stathmin family genes in zebrafish embryos | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳俊宏,郭典翰 | |
| dc.subject.keyword | 斑馬魚,胚胎, | zh_TW |
| dc.subject.keyword | zebrafish,stathmmin,embryo, | en |
| dc.relation.page | 51 | |
| dc.identifier.doi | 10.6342/NTU201600314 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2016-06-07 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生命科學系 | zh_TW |
| dc.date.embargo-lift | 2026-06-07 | - |
| 顯示於系所單位： | 生命科學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-105-1.pdf 此日期後於網路公開 2026-06-07 | 2.01 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。