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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 葉德銘(Der-Ming Yeh) | |
| dc.contributor.author | Yu-syuan Lin | en |
| dc.contributor.author | 林玗璇 | zh_TW |
| dc.date.accessioned | 2022-11-23T09:05:05Z | - |
| dc.date.available | 2024-09-30 | |
| dc.date.available | 2022-11-23T09:05:05Z | - |
| dc.date.copyright | 2021-11-03 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-09-12 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79607 | - |
| dc.description.abstract | "百子蓮(Agapanthus spp.) 為優良庭園植物，因具有夏季開花與罕見藍色花朵特性，目前已廣泛於景觀造園使用。然而臺灣對百子蓮物候適應性與耐熱性所知極少，本研究探討百子蓮於臺中新社區物候期特性，並以花粉、種子、細胞相對傷害值及葉綠素螢光值，探討測量品種於高溫逆境下之反應差異。 參試品種依開花時間區分為早生種：‘Big Blue’、‘Wolga’；中生種：‘Blue Baby’、‘Wavy Navy’、‘Purple Cloud’；晚生種：‘Hazy Day’、‘Storm Cloud’。百子蓮生長期具明顯季節性。物候現象約可分為七個階段，包括營養生長四個階段及生殖生長三個階段。所有品種的側芽萌生期約略落於八月至九月，常綠型大多於側芽萌生期後進入側芽葉片生長期，而落葉性品種則進入停滯期，此為兩品種群間之主要差異。常綠性品種之側芽葉片生長約於二月至四月間停止，進入停滯期，而落葉性品種約於二月重新開始生長，四月後生長速率逐漸趨緩。常綠性品種在花梗抽出前會出現約一個月的停滯期，並先後於四月底至六月中抽出花梗，而落葉性品種則約為六月底抽出花梗。 將八品種百子蓮花粉置於含 10% 蔗糖之BK培養基，以5℃為間隔，培養於水浴溫度5 - 45℃ 一小時，觀察溫度對花粉萌發率之影響，結果顯示百子蓮發芽率隨培養溫度上升逐漸增加，超過30°C則急劇下降。花粉發芽率具品種差異，以雙直線迴歸計算花粉發芽溫度三基點，最低發芽溫度(minimum temperature,Tmin)、最適發芽溫度(optimum temperature, Topt )及最高發芽溫度(maximum temperature, Tmax)分別為6℃、31.2℃、43.6℃。其中‘Wavy Navy’與‘Hazy Day’之Topt 較高，‘Purple Cloud’之Tmax較高，顯示此三品種可能具有耐熱潛力。 細胞電解質滲漏技術可用於量測細胞膜熱穩定性。以25、30、35、40、45、50、53、55、58、60、65或 70℃水浴溫度將8品種百子蓮葉片與花瓣圓片處理30分鐘後，所有品種的相對傷害值(relative injury, RI)隨水浴溫度增加而呈S曲線變化，葉片曲線轉折中點溫度約為53℃，以此水浴溫度進行品種耐熱性鑑定，結果顯示‘Blue Baby’ RI值最高，‘Wolga’、‘Storm Cloud’、‘Purple Cloud’及 ‘Wavy Navy’四品種較低；花瓣細胞相對傷害值反應與葉片接近，曲線轉折中點為50.1℃，顯示花瓣細胞對高溫更為敏感。 延續相對傷害值之試驗結果，挑選‘Blue Baby’、‘Big Blue’、‘Hazy Day’之種子，以5 - 25℃(每溫度處理間隔5℃)溫度處理。結果顯示5℃最大發芽率僅35%，隨溫度升高，各品種種子發芽率與發芽速率亦逐漸增加，僅‘Blue Baby’於25℃時發芽速率降低。‘Big Blue’及‘Hazy Day’發芽適溫為25℃，‘Blue Baby’則為20℃。依迴歸計算百子蓮發芽基礎溫度為1.9 - 2.3℃，種子發芽積溫為47 - 71℃d。 百子蓮‘Big Blue’以雙氧水與α-naphthyl acetate檢測柱頭活性，結果顯示百子蓮的柱頭於花開後第二天具可授粉性，為雌蕊後熟。以離體瓶插方式將百子蓮‘Big Blue’切花分別放置於平均溫度14、18、23、28及33℃ 溫控環境，於授粉後24小時調查體內花粉萌發情況，並記錄著果率、結種率與莢內種子數。‘Big Blue’體內花粉管於均溫23℃下皆生長正常，超過28℃高溫則出現花粉管末端膨大與伸長迴轉，並導致結實率與結籽率下降，顯示高溫造成百子蓮受精前障礙。 將三品種百子蓮栽培於日夜溫25/20與30/25℃環境。結果顯示‘Blue Baby’與‘Big Blue’之非光化學淬熄(non-photochemical quenching; qN)保持不變或增加，‘Hazy Day’則逐漸下降。25/20與30/25℃處理下，‘Hazy Day’之葉綠素螢光參數之最小螢光值(The minimal fluorescence in dark; Fo)、最大螢光釋放量(The maximum fluorescence in dark; Fm)及最大螢光參數的比值(maximum quantum efficiency of photosystem II, Fv/Fm)沒有明顯差異，而栽培於30/25℃ ‘Blue Baby’的Fv/Fm下降並維持於0.65，‘Big Blue’之Fv/Fm亦下降但逐漸回升，而‘Hazy Day’則維持於0.75-0.85間。‘Blue Baby’及‘Big Blue’於30/25℃下淨光合作用率降低，而‘Hazy Day’則無顯著差異。 百子蓮耐熱性品種選拔，建議的生理指標包括花粉最適發芽溫度(Topt)、葉片細胞相對傷害值(RI)，以及葉綠素螢光參數：qN、Fo、Fm、Fv/Fm。參試品種Hazy Day因可於炎熱的六月及七月生長開花，或可作為良好的耐熱候選品種。 " | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-23T09:05:05Z (GMT). No. of bitstreams: 1 U0001-1109202100535900.pdf: 2923936 bytes, checksum: 185b87ac4a35940d1d73df7592ef4f3f (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 摘要 .....i Abstract .....iv 目錄 (Contents) .....vii 表目錄 (List of Tables) .....ix 圖目錄 (List of Figures) .....xi 前言 (Introduction) .....1 前人研究 (Literature review) .....3 一、百子蓮簡介 .....3 (一)、分類與歷史 .....3 (二)、形態與生長習性 .....4 (三)、授粉與繁殖方式 .....4 二、栽培環境對百子蓮與其他球根花卉生長之影響 .....5 (一)、溫度 .....5 (二)、光度 .....6 (三)、肥培管理 .....6 三、 百子蓮與其他球根花卉之開花生理 .....6 (一)、溫度 .....6 (二)、光週期 .....7 (三)、植物生長激素與調節劑 .....8 四、花粉篩選於作物育種之應用 .....8 (一)、花粉活力與發芽檢測 .....9 (二)、花粉檢測於耐熱育種之運用 .....11 五、高溫逆境下植物熱逆境反應 .....12 (一)、外觀形態 .....12 (二)、微觀結構 .....13 (三)、生理指標 .....14 材料與方法 (Materials and Methods) .....17 試驗一、 百子蓮之物候現象調查 .....17 試驗二、溫度對百子蓮花粉發芽率之影響 .....17 試驗三、溫度對百子蓮葉片與花瓣細胞膜熱穩定性之影響 .....18 試驗四、溫度對百子蓮種子發芽之影響 .....19 試驗五、柱頭可授性與溫度對花粉發芽及結實之影響 .....20 試驗六、百子蓮實生苗於高溫逆境之反應 .....22 結果 (Results) .....25 試驗一、百子蓮之物候現象調查 .....25 試驗二、溫度對百子蓮花粉發芽率之影響 .....27 試驗三、溫度對百子蓮葉片與花瓣細胞膜熱穩定性之影響 .....28 試驗四、溫度對百子蓮種子發芽之影響 .....29 試驗五、柱頭可授性與溫度對花粉發芽及結實之影響 .....30 試驗六、百子蓮實生苗於高溫逆境之反應 .....31 討論 (Discussion) .....64 試驗一、百子蓮之物候現象調查 .....64 試驗二、溫度對百子蓮花粉發芽率之影響 .....66 試驗三、溫度對百子蓮葉片與花瓣細胞膜熱穩定性之影響 .....67 試驗四、溫度對百子蓮種子發芽之影響 .....69 試驗五、柱頭可授性與溫度對花粉發芽及結實之影響 .....71 試驗六、百子蓮實生苗於高溫逆境之反應 .....73 結論(Conclusion) .....78 參考文獻 (References) .....80 附錄(Appendix) .....96 | |
| dc.language.iso | zh-TW | |
| dc.title | 百子蓮之物候期與耐熱指標 | zh_TW |
| dc.title | Phenological phases and heat tolerant indicators of Agapanthus | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林淑怡(Hsin-Tsai Liu),許富鈞(Chih-Yang Tseng),朱玉 | |
| dc.subject.keyword | 百子蓮,物候期,耐熱,花粉,相對傷害值,細胞膜熱穩定性, | zh_TW |
| dc.subject.keyword | Agapanthus spp.,phenological phase,heat tolerance,pollen,relative injury value,cell membrane thermalstability, | en |
| dc.relation.page | 99 | |
| dc.identifier.doi | 10.6342/NTU202103118 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2021-09-13 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 園藝暨景觀學系 | zh_TW |
| dc.date.embargo-lift | 2024-09-30 | - |
| 顯示於系所單位： | 園藝暨景觀學系 | |
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