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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 鍾嘉綾(Chia-Lin Chung) | |
| dc.contributor.author | Ting-Zhi Liao | en |
| dc.contributor.author | 廖庭芝 | zh_TW |
| dc.date.accessioned | 2022-11-23T09:02:50Z | - |
| dc.date.available | 2026-09-29 | |
| dc.date.available | 2022-11-23T09:02:50Z | - |
| dc.date.copyright | 2021-10-04 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-09-29 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79527 | - |
| dc.description.abstract | "褐根病由木材白腐菌Phellinus noxius所引起，其在熱帶及亞熱帶地區可感染超過200種木本植物，遭受感染之樹木根部及莖基部腐朽易有傾倒之風險，造成潛在的公共安全問題。現今臺灣褐根病防治登記藥劑僅有撲克拉，此外，由於褐根病菌主要感染樹木根部，在防治時難以均勻地將殺菌劑施用在受感染組織，而為了達到最大之防治效果，以及避免長期使用單一藥劑導致抗藥性之發生，在選擇防治用藥時應篩選具有良好系統性且不同作用機制之殺菌劑。本研究首先將14支不同作用機制之殺菌劑，以0.1 ppm、1 ppm及10 ppm之含藥培養基，測試39株P. noxius菌株 [採集自臺灣 (4株)、香港 (4株)、馬來西亞 (4株)、澳洲 (5株) 及美屬太平洋群島 (16株)] 的抑制效果，結果顯示屬於Fungicide Resistance Action Committee所訂定作用機制 (Mode of Action, MoA) 中G1類之殺菌劑抑制效果最佳，尤其是環克座、得克利及依普座於1 ppm下可達97.7-99.8%之抑制率，而賽普護汰寧 (MoA-D1 + E2) 及滅普寧 (MoA-C2) 也具有良好之抑制效果，於10 ppm下分別具76.7-100%及79.1-100%之抑制率。另外，發現所有分離自American Samoa之褐根病菌株對滅普寧皆存在抗藥性，經解序後發現其在succinate dehydrogenase subunit C (SDHC) 胺基酸序列發生三個點突變，其中S85A及V173I可能為造成抗藥性發生之主因。為評估藥劑之移行性，透過根部浸泡及莖部注射之方式施用滅普寧、環克座、得克利、依普座、撲克拉及賽普護汰寧於茄苳 (Bischofia javanica) 實生苗進行測試，並於7天及21天後利用液相或氣相層析串聯式質譜儀進行殘留量分析，發現所有測試藥劑皆不具向下移行之能力，而環克座及得克利具有顯著較佳之向上移行性，依普座較弱，而撲克拉、滅普寧、賽普洛及護汰寧之移行能力則相當有限。而利用莖部注射之方式，於施藥後21天得克利、依普座及賽普洛向上移行能力顯著優於其他藥劑，可能由於依普座及賽普洛難以被根部吸收，導致其於根部浸泡系統中移行性不佳。最後透過上述六支殺菌劑及亞磷酸鉀的土壤澆灌並配合P. noxius 2248之人工莖部接種茄苳苗，評估殺菌劑藉由向上移行以防治褐根病之能力，發現在莖部變色面積、分離率及P. noxius菌量分析上，各個藥劑處理與對照組間並無顯著差異，可能與接種過於強勢有關，未來有待進一步以田間試驗評估藥劑在成樹之預防及治療效果。" | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-23T09:02:50Z (GMT). No. of bitstreams: 1 U0001-2609202122333400.pdf: 38358289 bytes, checksum: cb0e2e236b0ffd685ec65085eb495a82 (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 口試委員會審定書 # 誌謝 i 中文摘要 ii Abstract iv 目錄 vi 表目錄 ix 圖目錄 x 附錄目錄 xi 第一章、 前言 1 1.1 褐根病簡介 1 1.1.1 褐根病之重要性 2 1.1.2 現行防治方法 3 1.2 化學藥劑之系統性 5 1.2.1 影響化學藥劑移行之因素 5 1.2.2 移行性預測模型 7 1.3 化學藥劑之偵測方式 7 1.4 研究動機 8 第二章、 材料與方法 10 2.1 供試植物 10 2.2 供試菌株 10 2.2.1 菌株保存與培養 10 2.3 P. noxius對殺菌劑之敏感性測試 11 2.4 殺菌劑於樹苗之移行性評估 11 2.4.1 樹苗移行性評估系統之建立 11 2.4.2 藥劑於茄苳苗之向上移行性試驗 (根部浸泡) 12 2.4.3 藥劑於茄苳苗之上下移行性試驗 (莖部注射) 13 2.4.4 樣品前處理及藥劑殘留量分析 13 2.4.5 統計分析 14 2.5 殺菌劑對茄苳苗之藥害評估 15 2.6 溫室藥劑防治試驗 15 2.6.1 接種源製備及接種方法 16 2.6.2 P. noxius分離率 16 2.6.3 Quantitative Real-time PCR (qPCR) 17 2.6.4 接種部位之藥劑殘留量分析 18 2.6.5 統計分析 18 第三章、 結果 20 3.1 P. noxius對殺菌劑之敏感性測試 20 3.2 殺菌劑於樹苗之移行性評估 21 3.2.1 移行性系統之建立 21 3.2.2 藥劑於茄苳苗之向上移行性評估 (根部浸泡) 22 3.2.3 藥劑於茄苳苗之上下移行性評估 (莖部注射) 23 3.3 殺菌劑對茄苳苗之藥害評估 24 3.4 溫室藥劑防治試驗 24 3.4.1 萎凋率 24 3.4.2 莖部變色面積比率 25 3.4.3 P. noxius分離率 26 3.4.4 qPCR分析茄苳罹病組織內P. noxius菌量 26 3.4.5 接種部位之藥劑殘留量 27 第四章、 討論 28 4.1 P. noxius對殺菌劑之敏感性測試 28 4.2 滅普寧抗藥性分析 29 4.3 藥劑移行性測試系統之建立 30 4.4 殺菌劑於樹苗之移行性評估 32 4.4.1 提升化學藥劑於植物體內之移行性 34 4.5 殺菌劑對茄苳苗之藥害評估 35 4.6 溫室藥劑防治試驗 36 4.7 褐根病防治之建議 38 第五章、 參考文獻 40 表 52 圖 70 附錄 94 | |
| dc.language.iso | zh-TW | |
| dc.title | 殺菌劑之移行性及對樹木褐根病之防治效果 | zh_TW |
| dc.title | Translocation of fungicides and their efficacy on controlling brown root rot of trees | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 蔡志濃(Hsin-Tsai Liu),洪挺軒(Chih-Yang Tseng),黃姿碧,洪爭坊 | |
| dc.subject.keyword | 褐根病,化學防治,殺菌劑,移行性, | zh_TW |
| dc.subject.keyword | brown root rot,chemical control,fungicide,translocation, | en |
| dc.relation.page | 125 | |
| dc.identifier.doi | 10.6342/NTU202103375 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2021-10-01 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 植物醫學碩士學位學程 | zh_TW |
| dc.date.embargo-lift | 2026-09-29 | - |
| 顯示於系所單位： | 植物醫學碩士學位學程 | |
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