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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79409完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 嚴震東(Chen-Tung Yen) | |
| dc.contributor.author | Yu-Wen Liu | en |
| dc.contributor.author | 劉聿彣 | zh_TW |
| dc.date.accessioned | 2022-11-23T08:59:51Z | - |
| dc.date.available | 2021-11-03 | |
| dc.date.available | 2022-11-23T08:59:51Z | - |
| dc.date.copyright | 2021-11-03 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-10-21 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79409 | - |
| dc.description.abstract | 感覺神經負責將個體從環境中接收到的刺激轉化為電訊號，然後將它們傳送到中樞神經系統。在我們的皮膚中，有多種類型的感覺神經來偵測多種不同類型的環境變化，這些不同類型的神經上具有各種受體分子、離子通道等等不同組合，而每一種構造都有其負責的感覺功能。舉例來說，TRPV1 和 MrgD 都屬於皮下神經的 C 纖維。 TRPV1 是負責偵測有害熱和痛的神經，其活化劑是辣椒素。 MrgD是偵測瘙癢和疼痛的神經，它的活化劑是β-丙氨酸。識別特定類型的感覺神經可以幫助我們進行臨床和科學研究，但目前缺乏有效的方法來識別它們。若有一種方法可以幫助我們在活體內觀察不同功能類型的感覺神經，並可隨時在體內追蹤這些神經纖維，將會是非常有趣且實用的，因此我們的目標是開發一種顯微神經測定法來選擇性地標記特定類型的神經纖維。 在本研究中，我們利用上述特定感覺具有特定受體分子或離子通道的特性，並使用小分子染料亞甲藍來標記以活化劑活化的特定感覺神經來標記活體中的皮下神經。此外，我們使用 TRPV1-tdTomato 和 MrgD-GFP 轉基因小鼠來測試我們的概念。 我們的結果分為兩個部分。第一部分是我們利用共軛焦顯微鏡觀察 TRPV1-tdTomato 和 MrgD-GFP 小鼠後腳掌的皮下神經纖維形態。第二部分是亞甲藍注射到小鼠腳掌的結果。我們的結果表明，活化劑與亞甲藍的組合增加了亞甲藍標記特定種類神經的比例。也就是說，同時注射β-丙氨酸和亞甲藍會使亞甲藍更傾向標記MrgD神經，而同時注射辣椒素和亞甲藍會使亞甲藍傾向標記TRPV1神經。 綜合上述研究，我們發現通過共軛交顯微鏡來活體掃描小鼠可以幫助我們觀察和操縱生物體內的神經纖維。此外，共同注射 MB 和活化劑可以增強亞甲藍標記特定種類的神經纖維。這些方法使我們能夠在體內觀察小鼠的皮下特定神經。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-23T08:59:51Z (GMT). No. of bitstreams: 1 U0001-2010202110303700.pdf: 4814581 bytes, checksum: 13d0530ce393da16472f474b4c11b085 (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | CONTENTS CONTENTS OF FIGURES 3 TABLE OF ABBREVIATIONS 5 摘要 6 ABSTRACT 8 1.INTRODUCTION 10 1.1. Various type of cutaneous nerve fibers 10 1.2. Basic hypothesis 13 1.3. Staining of nerve fiber 13 1.4. TRPV1 and MrgD mice 14 1.5. Specific objectives of the present study 16 2. MATERIALS AND METHODS 17 2.1. Animal 17 2.2. MB and MB-ligand mixture preparation 17 2.3. MB injection 18 2.4. Intravital confocal microscopy imaging 18 2.5. Image analysis 19 2.5.1. Colocalization of MB and TRPV1/MrgD fibers 19 2.5.2. MB length/volume analysis 20 2.5.3. Percentage that MrgD fibers labeled by MB 22 3. RESULTS 23 3.1. Morphology of cutaneous TRPV1 fibers 23 3.2. MB labeling TRPV1 fibers 23 3.3. Morphology of MrgD 24 3.4. MB labeling MrgD fibers 25 3.5. Negative control of MB labeling TRPV1 and MrgD fibers 26 4. DISCUSSIONS 27 5. FIGURES 30 6. REFERENCES 51 | |
| dc.language.iso | en | |
| dc.title | 以活化方式增強標記小鼠皮下特定神經 | zh_TW |
| dc.title | Activation-Enhanced Labeling of Type Specific Cutaneous Nerve Fibers | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳示國(Hsin-Tsai Liu),林文瑛(Chih-Yang Tseng),陳志成 | |
| dc.subject.keyword | 亞甲藍,TRPV1,MrgD,活體影像,小鼠,皮下神經纖維, | zh_TW |
| dc.subject.keyword | methylene blue (MB),TRPV1,MrgD,mice,intravital imaging,cutaneous nerve fibers, | en |
| dc.relation.page | 57 | |
| dc.identifier.doi | 10.6342/NTU202103916 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2021-10-22 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生命科學系 | zh_TW |
| 顯示於系所單位： | 生命科學系 | |
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