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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 柯佳吟(Chia-Ying Ko) | |
| dc.contributor.author | Shih-Han Wen | en |
| dc.contributor.author | 溫士瀚 | zh_TW |
| dc.date.accessioned | 2022-11-23T08:58:47Z | - |
| dc.date.available | 2024-10-26 | |
| dc.date.available | 2022-11-23T08:58:47Z | - |
| dc.date.copyright | 2021-11-03 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-10-27 | |
| dc.identifier.citation | Ainsworth, T. D., Krause, L., Bridge, T., Torda, G., Raina, J. B., Zakrzewski, M., Gates, R. D., Padilla-Gamino, J. L., Spalding, H. L., Smith, C., Woolsey, E. S., Bourne, D. G., Bongaerts, P., Hoegh-Guldberg, O., Leggat, W. (2015). The coral core microbiome identifies rare bacterial taxa as ubiquitous endosymbionts. Isme Journal, 9(10), 2261-2274. Baker, D. M., Freeman, C. J., Wong, J. C. Y., Fogel, M. L., Knowlton, N. (2018). Climate change promotes parasitism in a coral symbiosis. Isme Journal, 12(3), 921-930. Ben-Haim, Y., Thompson, F. L., Thompson, C. C., Cnockaert, M. C., Hoste, B., Swings, J., Rosenberg, E. (2003). Vibrio coralliilyticus sp nov., a temperature-dependent pathogen of the coral Pocillopora damicornis. International Journal of Systematic and Evolutionary Microbiology, 53, 309-315. Berger, M., Neumann, A., Schulz, S., Simon, M., Brinkhoff, T. (2011). 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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79357 | - |
| dc.description.abstract | 珊瑚礁是海洋中生物多樣性最高的生態系之一,當中為生態系關鍵物種的珊瑚,其研究格外受注目。近年來因人類活動導致全球氣候改變,多數研究指出海洋動物會隨著環境改變而遷移到適合生存的環境,但採取固著型生活方式的珊瑚卻難以主動遷移棲地,而可能是以改變其共棲微生物的組成來適應環境上的變動。過往研究已知環境因子的改變,像是溫度,會對珊瑚共棲的微生物族群造成影響,其中珊瑚共棲菌是由珊瑚宿主還是環境主導仍還不夠清楚。台灣位於東亞季風帶上及橫跨熱帶及亞熱帶,並且部分海域為黑潮流經處,有著差異甚大的海洋環境,產生了多樣的珊瑚礁棲地。因此,本研究以調查石珊瑚之菌相在不同珊瑚礁棲地中的差異,希望了解主導珊瑚共棲菌的是宿主還是環境。我們針對台灣亞熱帶(東北角)與熱帶(綠島)兩處,於兩季節(冬末/夏末) 採集兩種台灣常見石珊瑚–萼柱珊瑚 (Stylophora pistillata) 及鐘形微孔珊瑚 (Porites lutea)其中綠島更針對不同深度 (10/40米)進行比較,抽取珊瑚體上的DNA,針對細菌之16S rDNA分析珊瑚共棲菌相組成差異,並結合各項環境參數和珊瑚生理數值,分析與菌相差異的關聯性。根據結果得知珊瑚宿主差異是最主要影響共棲菌相組成的因子,其次為地點之差異,接著是季節與深度等因素。從本研究的結果了解,石珊瑚的共棲菌組成結構可能是由珊瑚宿主主導。另外,藉由了解台灣東北角及綠島珊瑚共棲菌相的差異性,可幫助日後研究不同季風帶之珊瑚共棲體適應環境變動時的策略。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-23T08:58:47Z (GMT). No. of bitstreams: 1 U0001-2610202111312400.pdf: 3258669 bytes, checksum: 12e4abc6f1f03a6bf734e1d38f302d6e (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 口試委員會審定書………………………………………………………ii 致謝……………………………………………………………………...iii 中文摘要....……………………………………………………………...iv Abstract……………………………………………………………….......v 表目錄………………………………………………………………….viii 圖目錄…………………………………………………………………....x 前言………………………………………………………………………1 材料與方法………………………………………………………………6 採樣地點及樣本保存……………………………………………….6 去氧核醣核酸萃取………………………………………………….6 DNA品質測量………………………………………………………7 聚合酶連鎖反應……………………….……………………………7 DNA純化……………………………………………………………8 條碼標記之聚合酶連鎖反應……………………………………….9 DNA純化及濃縮……………………………………………………9 DNA定序以及分析處理……………………………………………9 統計分析…………………………………………………………...10 結果……………………………………………………………………..12 珊瑚共棲菌相分析………………………………………………...12 菌相與環境因子之關聯性分析………….. ………………………14 菌相與生理資料之關聯性分析…………………………………...16 討論……………………………………………………………………..18 菌相由珊瑚宿主主導其次為環境差異…………………………...18 珊瑚菌相的結構…………………………………………………...21 珊瑚菌相與環境因子的關係……………………………………...22 珊瑚菌相與生理資料的關係……………………………………...23 結論……………………………………………………………………..25 參考資料………………………………………………………………..84 附錄……………………………………………………………………..92 | |
| dc.language.iso | zh-TW | |
| dc.title | 探討地點、季節與深度對鐘形微孔珊瑚及萼形柱珊瑚的共棲菌相影響 | zh_TW |
| dc.title | "Influences of location, season and depth on coral-associated bacterial composition in Porites lutea and Stylophora pistillata" | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 楊姍樺(Shan-Hua Yang) | |
| dc.contributor.oralexamcommittee | 湯森林(Hsin-Tsai Liu),(Chih-Yang Tseng) | |
| dc.subject.keyword | 珊瑚共棲菌,萼柱珊瑚,鐘形微孔珊瑚,16S rDNA,環境因子,宿主專一, | zh_TW |
| dc.subject.keyword | coral microbiome,Stylophora pistillata,Porites lutea,16S rDNA,environmental factors,host specificity, | en |
| dc.relation.page | 114 | |
| dc.identifier.doi | 10.6342/NTU202104211 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2021-10-28 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| dc.date.embargo-lift | 2024-10-26 | - |
| 顯示於系所單位: | 漁業科學研究所 | |
文件中的檔案:
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| U0001-2610202111312400.pdf | 3.18 MB | Adobe PDF | 檢視/開啟 |
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