請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79355完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 王永松(Yung-Song Wang) | |
| dc.contributor.author | Tai-Yuan Chen | en |
| dc.contributor.author | 陳泰元 | zh_TW |
| dc.date.accessioned | 2022-11-23T08:58:45Z | - |
| dc.date.available | 2021-11-03 | |
| dc.date.available | 2022-11-23T08:58:45Z | - |
| dc.date.copyright | 2021-11-03 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-10-26 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79355 | - |
| dc.description.abstract | 金目鱸，又稱尖吻鱸，為一種廣鹽性魚類，分佈於印度-西太平洋、臺灣、東南亞、澳洲一帶，在臺灣廣泛養殖於南部地區，作為臺灣重要的經濟魚種，目前缺少其系群管理、受精卵及魚苗來源等相關的研究，為改善並篩選優勢種苗以長期維持養殖品質，評估養殖金目鱸的族群遺傳資訊為首要步驟，本研究使用粒線體DNA中Cyt b、COI以及核DNA中的微衛星基因座作為遺傳標記分析臺灣金目鱸三養殖族群（嘉義養殖場A池、嘉義養殖場B池、臺南養殖場）及淡水河口族群的遺傳相關資訊，其中以粒線體DNA物種鑑定結果顯示現存臺灣的金目鱸中存在兩物種(Lates calcarifer與Lates uwisara)，且此兩物種無法在形質特徵上區別。本研究分析樣本中L. calcarifer的遺傳多樣性及遺傳結構，粒線體DNA分析結果中顯示，除了嘉義B養殖族群外，各族群的單倍型多樣性高以及核苷酸多樣性低的情形，單倍型網絡圖中，各族群共享祖先單倍型的同時仍各自具有特有單倍型，遺傳分化指數(ΦST)結果顯示族群間具一定遺傳差異；而微衛星基因座分析結果中，各族群均具相當程度的遺傳多樣性，此外，以微衛星作為標記進行主座標軸分析、貝氏分類指派、遺傳分化指數解析各族群間的遺傳結構關係，結果顯示各族群僅有低度分化程度，部分族群呈現彼此混合但仍具一定的遺傳差異，基於養殖場間理應不具基因交流，本研究推測各養殖場間可能具交換種苗的行為。 本研究檢視淡水河口捕獲的金目鱸胃內容物並分析耳石穩定性碳及氧同位素以探討養殖與野生金目鱸的生活史。淡水河口捕獲的樣本其同位素值範圍較為廣泛，而養殖的樣本於生命各階段皆呈現相近且具規律的同位素值變化，此外，以氧同位素分化公式估算魚體經歷的鹽度變化，結果顯示部分淡水野生個體在各時期呈現規律性的遷移，但個體間經歷的鹽度差異大，表示金目鱸棲地利用具個體間差異，從仔稚魚期的鹽度研判，海水水域以及淡水環境都可能是其產卵場。二次判別分析顯示各養殖場間在耳石同位素值具顯著差異，且此差異可於判別分析中達到至高95%的準確率。本研究以碳氧穩定性同位素能有效建立金目鱸的棲地利用並辨別養殖與野生個體，可供未來金目鱸漁業資源管理及評估相關政策時做為參考依據。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-23T08:58:45Z (GMT). No. of bitstreams: 1 U0001-2510202112121100.pdf: 5739683 bytes, checksum: d8d024897273374b8ea49c184683aa11 (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 摘要 i Abstract ii 表目錄 v 圖目錄 vi 一、 前言 7 1.1. 金目鱸(Lates calcarifer) 7 1.2. 養殖現況與問題 7 1.3. COI、Cyt b及微衛星(microsatellite) 8 1.4. 耳石及魚類生活史 9 1.5. 研究動機與目的 10 二、 材料方法 11 2.1 樣本採集 11 2.2 物種鑑定 11 2.3 胃內容物分析 12 2.3.1 出現率法(Occurrence method) 12 2.3.2 豐富度法(Numerical method) 12 2.3.3 重量法(Gravimetric method) 12 2.4 DNA萃取及遺傳標記(Genetic marker)篩選 12 2.5 聚合酶鏈鎖反應(Polymerase Chain Reaction) 13 2.6 親緣關係及族群遺傳結構分析 13 2.6.1 粒線體DNA(COI cyt b) 13 2.6.2 微衛星基因座(Microsatellite loci) 16 2.7 耳石樣本處理及分析 17 2.7.1. 耳石包埋與研磨 17 2.7.2. 穩定碳、氧同位素分析 18 2.7.3. 鹽度估計時序變化圖 18 2.7.4. 統計與二次判別分析 19 三、 結果 21 3.1 物種鑑定 21 3.2 胃內容物分析 21 3.3 DNA萃取及遺傳標記篩選 22 3.4 粒線體DNA (COI Cyt b) 22 3.4.1. 親緣關係分析 22 3.4.2. 遺傳多樣性 22 3.4.3. 族群遺傳結構 23 3.4.4. AMOVA 23 3.4.5. 族群狀態分析 24 3.5 微衛星 24 3.5.1. 基因型分型(Genotyping)及資料分析 24 3.5.2. 族群遺傳結構 24 3.6 耳石同位素值分析 25 3.6.1. 同位素值—生長時序變化圖 25 3.6.2. 耳石碳、氧同位素值統計分析 26 3.6.3. 估計鹽度時序變化圖 28 3.6.4. 二次判別分析 28 四、 討論 29 4.1. 隱蔽種的存在 29 4.2. 各族群遺傳多樣性及族群遺傳結構探討 30 4.3. 族群狀態探討 32 4.4. 耳石碳氧同位素 33 4.5. 鹽度史重建 35 4.6. 養殖與野生個體之判別 36 五、 結論 38 六、 參考文獻 39 | |
| dc.language.iso | zh-TW | |
| dc.title | 臺灣金目鱸屬(Lates)物種鑑定、族群遺傳結構分析及辨別養殖與野生個體 | zh_TW |
| dc.title | "Lates species identification, population genetic structure analysis and discrimination from wild and cultured populations in Taiwan" | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 蕭仁傑(Jen-Chieh Shiao) | |
| dc.contributor.oralexamcommittee | 陳韋仁(Hsin-Tsai Liu),林洪君(Chih-Yang Tseng) | |
| dc.subject.keyword | 金目鱸,微衛星,耳石穩定性同位素,族群遺傳結構, | zh_TW |
| dc.subject.keyword | Lates calcarifer,Lates uwisara,mircrosatellite,otolith stable isotope,population genetic strcture, | en |
| dc.relation.page | 93 | |
| dc.identifier.doi | 10.6342/NTU202104129 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2021-10-27 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
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