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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李昆達(Kung-Ta Lee) | |
| dc.contributor.author | Han-Jung Kuo | en |
| dc.contributor.author | 郭翰蓉 | zh_TW |
| dc.date.accessioned | 2022-11-23T08:58:22Z | - |
| dc.date.available | 2021-11-04 | |
| dc.date.available | 2022-11-23T08:58:22Z | - |
| dc.date.copyright | 2021-11-04 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-10-29 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79336 | - |
| dc.description.abstract | 根毛農桿菌會將T-DNA插入植物染色體並誘導植物生成毛狀根，T-DNA上的rolB與rolC基因在毛狀根誘導與側根生長中扮演重要角色。RolC之交互作用蛋白篩選無法在現成之酵母菌雙雜合系統中進行，因為RolC蛋白本身會自活化影響篩選，因而建立一系列的RolC突變，篩選具有較低自活化能力之突變。A119K+P120D突變有最低的自活化能力，N91K與G76K亦具有不錯之效果，將A119K+P120D突變RolC置換回根毛農桿菌中，其生長與野生型農桿菌相同，另外也以突變RolC農桿菌誘導菸草毛狀根，其根生長形態與野生型相近。接著以A119K+P120D突變RolC進行酵母菌雙雜合篩選，發現兩個可能與RolC有交互作有的蛋白質，分別為quinolinate合成酶與延伸因子1a (elongation factor 1-a)，在酵母菌單雜交實驗中發現延伸因子1可能與RolC有交互作用，後續將進一步驗證此交互作用之真偽。 在先前研究中本實驗室發現一群可能受rolB或rolC調控之脂質轉移蛋白(LTP)，找出這些LTP全長cDNA並檢驗這些LTP、rolB與rolC在不同荷爾蒙處理之毛狀根中的表現，我們發現LTP之表現與rolB或rolC之表現關聯性並不大，顯示LTP可能並不直接受到rolB或rolC之調控。而LTP6與LTP7表現量調降之毛狀根，與rolB或rolC缺失之毛狀根有類似的側根生長抑制之現象。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-23T08:58:22Z (GMT). No. of bitstreams: 1 U0001-2710202102504400.pdf: 2901113 bytes, checksum: d55475badbd65806a19cee78bbf1933b (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 摘要 i Abstract ii Content iv List of Figures vii List of Table ix Abbreviations x Introduction 1 Agrobacterium rhizogenes caused hairy root syndrome in plants 1 rolC plays crucial role in hairy root formation and growth 3 Lipid transfer proteins are complicated and widely existed in plants 5 Materials and Methods 8 Bacteria and plant materials 8 Tobacco hairy root induction 8 Confirmation of the genotypes of the hairy roots 9 Phytohormone treatments on hairy root 10 Mutant RolC selection 10 Construction of A. rhizogenes containing mutant RolC 11 Yeast transformation 12 Yeast two-hybrid screening 13 Confirmation of interaction between RolC and candidate proteins 14 Identification of the full-length sequences of tobacco lipid transfer proteins 14 Results 16 Leaf discs in the same space during infection may influence hairy root induction 16 RolC mutants for yeast two-hybrid screening 18 Screening of mutant RolC 18 The growth of A. rhizogenes containing rolC mutants was not influenced 20 The growth of tobacco hairy root containing rolC mutants 21 RolC yeast two-hybridization screening 22 The interaction between candidate proteins and RolC confirmation by single mating 23 Lipid transfer proteins in tobacco hairy root 25 LTPs sequence analysis 25 The expression patterns of LTPs were not correlated with rolB/rolC 26 LTP6/LTP7 knock-down hairy root showed lateral root growth inhibition which was similar to rolB/rolC deficient mutant 28 Discussion 30 References 68 Appendix 76 | |
| dc.language.iso | en | |
| dc.title | 菸草毛狀根形成機制之探討 | zh_TW |
| dc.title | The Study on Formation Mechanism of Tobacco Hairy Root | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 博士 | |
| dc.contributor.oralexamcommittee | 楊健志(Hsin-Tsai Liu),劉啟德(Chih-Yang Tseng),林乃君,陳賢明 | |
| dc.subject.keyword | 根毛農桿菌,毛狀根,rolC,酵母菌雙雜合系統,脂質轉移蛋白, | zh_TW |
| dc.subject.keyword | Agrobacterium rhizogenes,hairy root,rolC,yeast two-hybrid,lipid transfer proteins, | en |
| dc.relation.page | 79 | |
| dc.identifier.doi | 10.6342/NTU202104293 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2021-10-29 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| 顯示於系所單位： | 生化科技學系 | |
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