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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79323完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳示國(Shih-Kuo CHen) | |
| dc.contributor.author | Chieh-Wen Ho | en |
| dc.contributor.author | 何介文 | zh_TW |
| dc.date.accessioned | 2022-11-23T08:58:07Z | - |
| dc.date.available | 2021-11-04 | |
| dc.date.available | 2022-11-23T08:58:07Z | - |
| dc.date.copyright | 2021-11-04 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-10-30 | |
| dc.identifier.citation | [1] Hansen J. Increased breast cancer risk among women who work predominantly at night. Epidemiology. 2001 Jan;12(1):74-7. [2] Noh J. The Effect of Circadian and Sleep Disruptions on Obesity Risk. J Obes Metab Syndr. 2018 Jun 30;27(2):78-83. [3] Dennis C Whitehead, Harold Thomas, Debra Roberts Slapper, A rational approach to shift work in emergency medicine, Annals of Emergency Medicine, Volume 21, Issue 10, 1992, Pages 1250-1258 [4] Shimizu K, Fukada Y. Stereotaxic surgery for suprachiasmatic nucleus lesions in mice. Bio Protoc. 2017 Jun 20;7:e2346. [5] Rosenwasser, Alan. Functional neuroanatomy of sleep and circadian rhythms. Brain Research Reviews. 61. 281-306. 10.1016/j.brainresrev.2009.08.001. [6] Hafner M, Koeppl H, Gonze D, Effect of Network Architecture on Synchronization and Entrainment Properties of the Circadian Oscillations in the Suprachiasmatic Nucleus. PLOS Computational Biology 8(3): e1002419, 2012 [7] Ecker JL, Dumitrescu ON, Wong KY, Alam NM, Chen SK, LeGates T, Renna JM, Prusky GT, Berson DM, Hattar S. Melanopsin-expressing retinal ganglion-cell photoreceptors: cellular diversity and role in pattern vision. Neuron. 2010 Jul 15;67(1):49-60. [8] Fernandez DC, Chang YT, Hattar S, Chen SK. Architecture of retinal projections to the central circadian pacemaker. Proc Natl Acad Sci U S A. 2016 May 24;113(21):6047-52. [9] Colwell CS. NMDA-evoked calcium transients and currents in the suprachiasmatic nucleus: gating by the circadian system. Eur J Neurosci. 2001;13(7):1420-1428. [10] M.E. Flores-Soto et al., 'Structure and function of NMDA-type glutamate receptor subunits', Neurología (English Edition), Volume 27, Issue 5, Pages 301-310, 2012 June [11] Paoletti, P., Bellone, C. Zhou, Q. NMDA receptor subunit diversity: impact on receptor properties, synaptic plasticity and disease. Nat Rev Neurosci 14, 383–400 (2013). [12] ISH Data :: Allen Brain Atlas: Mouse Brain, https://mouse.brain-map.org/ [13] Hatori M, Le H, Vollmers C, Keding SR, Tanaka N, et al. 'Inducible Ablation of Melanopsin-Expressing Retinal Ganglion Cells Reveals Their Central Role in Non-Image Forming Visual Responses.', PLOS ONE 3(6): e2451, 2008 [14] Brancaccio, Marco et al. “Astrocytes Control Circadian Timekeeping in the Suprachiasmatic Nucleus via Glutamatergic Signaling.” Neuron vol. 93,6 (2017): 1420-1435.e5. (2017) | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79323 | - |
| dc.description.abstract | "視交叉上核(suprachiasmatic nucleus, SCN)是動物生理週期的調控核心，在光週期下會接收外來刺激，經內生感光視神經節細胞(intrinsically photosensitive retinal ganglion cell，ipRGC)藉glutamate送到SCN而產生生理週期。SCN細胞分為腹側的VIP和GRP，及背側的AVP細胞。過去認為ipRGC主要將訊息送至VIP，然而2016年發現，ipRGC也可以與AVP細胞產生synapse，且連結在AVP細胞本體上突觸數量較VIP和GRP細胞更多，和過去的假說並不一致。另外，先前研究顯示SCN的背側特別表現了NMDA receptor subunit的NR2C。因此，本研究為探討ipRGC在SCN背側訊息傳導對於生理時鐘調控的影響，利用CRISPR包裹於AAV藉由steriotaxis micro-injection打入wild type以及cre-dependent老鼠SCN中knock-out該處的NR2C，再觀察老鼠進行wheel-running時jet-lag、masking、phase shift，以及在skeleton photo period下生理時鐘的改變。我們發現，在SCN的NMDA受器被knock-out的老鼠在光masking效果變差，與phase shift增加。而在AVP細胞上專一性knock-out掉NMDA受器的老鼠的改變則不明顯，推測非AVP cell表現之NR2C與photoentrainment是有關係的。" | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-23T08:58:07Z (GMT). No. of bitstreams: 1 U0001-2710202112234100.pdf: 39600408 bytes, checksum: 62b904b8a5bfd7b3f8644e823a0d8a54 (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 口試委員審定書 ………………………………………………….. i 致謝 ……………………………………………………………….. ii 中文摘要 ………………………………………………………….. iii Abstract …………………………………………………………… iv Content ……………………………………………………………. v Chapter 1. Introduction …………………………………………... 1 1.1 Statement of purpose……………………………….…... 3 Chapter 2. Material and methods ………………………………. 4 2.1 CRISPR plasmid cloning and AAV packaging ………….. 4 2.2 Animals ..................…………………………...…………. 4 2.3 Stereotactic micro-injection surgery ……………………. 5 2.4 Wheel-running data recording …………………………. 5 2.5 Brain tissue harvesting and frozen section …………….... 6 2.6 Immunohistochemistry …………………………….... 6 2.7 Statistic ……………………………………………….... 6 Chapter 3. Results ……………………………………………….... 7 3.1 NR2C KO in mice dorsal SCN ……………………….. 7 3.2 Jetlag entrainment is not changed in nonspecific KO mice …………………………………...…………………. 8 3.3 Masking is altered in non-specific KO mice …………..... 8 3.4 Phase shift is altered in non-specific KO mice under constant dark ……………………………………………..... 9 3.5 Entrainment of non-specific KO mice under skeleton photoperiod is not changed ………………………………… 10 3.6 Jetlag entrainment is not changed in specific knock-out mice ………………………………………………………… 10 3.7 Masking is not altered in specific KO mice …………… 11 3.8 Phase shift is not altered in specific KO mice ………… 11 3.9 Entrainment of specific KO mice under skeleton ……… 11 Chapter 4. Discussions …………………………………………… 13 4.1 Significance of the work…………………………….…… 15 Figures ……………………………………………………………... 17 References …………………………………………………………. 27 Appendix ………………………………………………………….. 29 | |
| dc.language.iso | en | |
| dc.title | 麩胺酸受器次單元NR2C在視交叉上核對於光線調控生理週期所扮演的角色 | zh_TW |
| dc.title | The role of NMDA receptor 2C subunit in suprachiasmatic nucleus for circadian photoentrainment | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 明智煥(Hsin-Tsai Liu),閔明源(Chih-Yang Tseng),張芳嘉 | |
| dc.subject.keyword | 生理時鐘,光,麩胺酸受器, | zh_TW |
| dc.subject.keyword | Circadian rhythm,NMDAR2C,photoentrainment, | en |
| dc.relation.page | 60 | |
| dc.identifier.doi | 10.6342/NTU202104326 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2021-10-30 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生命科學系 | zh_TW |
| 顯示於系所單位： | 生命科學系 | |
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| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| U0001-2710202112234100.pdf | 38.67 MB | Adobe PDF | 檢視/開啟 |
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