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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 胡凱康(Kae-Kang Hwu) | |
| dc.contributor.author | Meng-Ting Hsieh | en |
| dc.contributor.author | 謝孟婷 | zh_TW |
| dc.date.accessioned | 2022-11-23T08:56:36Z | - |
| dc.date.available | 2022-02-21 | |
| dc.date.available | 2022-11-23T08:56:36Z | - |
| dc.date.copyright | 2022-02-21 | |
| dc.date.issued | 2022 | |
| dc.date.submitted | 2022-01-19 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79246 | - |
| dc.description.abstract | "過去在水稻秈稉雜交F2族群第三條與第六條染色體中觀察到不平衡分離的現象，推測各存在至少一個不平衡分離基因座 (segregation distortion locus, SDL)，並利用正反回交族群確認為雄配子體效應，又透過花粉染色實驗發現沒有花粉不稔的現象，推測其效應為雄配子體競爭 (male gamete competition)，命名為Mgc3與Mgc6。本研究使用水稻秈稉雜交重組自交系 (recombinant inbred lines, RILs) F8:9世代基因型資料初步定位Mgc3與Mgc6及選拔家系建立回交族群，利用分子標誌逐步縮小預期區間，最終將Mgc3與Mgc6精細定位於第三條染色體13,260,366-13,774,613 bp與第六條染色體30,399,261-30,957,282 bp處，分別長514,247 bp與558,021 bp。透過混合花粉對偶基因分離比檢測排除染色體驅動的可能，推論Mgc3與Mgc6效應為花粉競爭，並對效應進行探討。" | zh_TW |
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| dc.description.tableofcontents | 目錄 誌謝 i 摘要 ii Abstract iii 目錄 iv 圖目錄 vi 表目錄 vii 第一章 前言 1 第二章 前人研究 4 2-1、 不平衡分離 4 2-2、 花粉競爭 5 2-3、 不平衡分離與族群結構 6 2-4、 定位不平衡分離基因座 7 2-5、 水稻秈稉雜交族群 10 第三章 材料與方法 12 3-1、 遺傳材料與精細定位 12 3-2、 DNA萃取與定量 15 3-3、 定序資料 16 3-4、 異質結合個體花粉對偶基因頻度測定 18 3-5、 分子標誌設計與基因型鑑定 18 3-6、 族群模擬 20 第四章 結果 22 4-1、 試驗族群推進 22 4-2、 Mgc3精細定位 23 4-3、 Mgc6精細定位 27 4-4、 兩SDL同為異質結合家系 30 4-5、 混合花粉對偶基因分離比 32 4-6、 Mgc3與Mgc6的效應 34 第五章 討論 35 5-1、 Mgc3與Mgc6的精細定位 35 5-2、 Mgc3與Mgc6的效應與交互作用 37 參考文獻 40 附錄一、預期區間內的候選基因 48 附錄二、分子標誌序列Custom Assay ID 52 圖目錄 圖一、 試驗族群建立流程圖 14 圖二、 家系譜系表 22 圖三、 Mgc3精細定位過程 26 圖四、 Mgc6精細定位過程 29 圖五、 026-4-01-004-11花粉與對照樣品以S3_ 13928023檢驗結果 33 表目錄 表一、 回交家系與回交種子檢驗 23 表二、 Mgc3自交後裔檢定結果 25 表三、 Mgc6自交後裔檢定結果 28 表四、 Mgc3與Mgc6基因型同為ij家系分離比 31 表五、 族群擬結果 34 | |
| dc.language.iso | zh-TW | |
| dc.title | 精細定位水稻秈稉雜交雄配子體競爭Mgc3與Mgc6基因 | zh_TW |
| dc.title | Fine mapping male gamete competition Mgc3 and Mgc6 gene in rice (Oryza sativa L.) | en |
| dc.date.schoolyear | 110-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳凱儀(Wen-Yau Liang),黃永芬(Wei-Chung Hsu) | |
| dc.subject.keyword | 不平衡分離,精細定位,雄配子體競爭,花粉競爭,族群模擬, | zh_TW |
| dc.subject.keyword | segregation distortion,fine mapping,male gamete competition,pollen competition,simulation, | en |
| dc.relation.page | 55 | |
| dc.identifier.doi | 10.6342/NTU202200087 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2022-01-19 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 農藝學研究所 | zh_TW |
| 顯示於系所單位： | 農藝學系 | |
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