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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 楊台鴻(Tai-Horng Young) | |
dc.contributor.author | Yu-Ting Wang | en |
dc.contributor.author | 王聿廷 | zh_TW |
dc.date.accessioned | 2021-07-11T15:49:42Z | - |
dc.date.available | 2023-08-02 | |
dc.date.copyright | 2018-08-02 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-07-30 | |
dc.identifier.citation | 1. Bommart, S., et al., Imaging of postoperative complications following surgery for lung cancer. Diagn Interv Imaging, 2017. 98(1): p. 11-20.
2. Reza John Mehran, M., Lung Cancer- An Update on Current Surgical Strategies. Texas Heart Institute Journal, 2012. 39(6). 3. Brocki, B.C., et al., Decrease in pulmonary function and oxygenation after lung resection. ERJ Open Res, 2018. 4(1). 4. Mueller, M.R. and B.A. Marzluf, The anticipation and management of air leaks and residual spaces post lung resection. J Thorac Dis, 2014. 6(3): p. 271-84. 5. Yoo, A., et al., Burden of air leak complications in thoracic surgery estimated using a national hospital billing database. Clinicoecon Outcomes Res, 2017. 9: p. 373-383. 6. Dugan, K.C., et al., Management of Persistent Air Leaks. Chest, 2017. 152(2): p. 417-423. 7. John C. Wain, M., et al., Trial of a Novel Synthetic Sealant in Preventing Air Leaks After Lung Resection. The Society of Thoracic Surgeons, 2001. 71. 8. Cagirici, U., et al., Experimental use of N-butyl cyanoacrylate tissue adhesive on lung parenchyma after pulmonary resection. Thorac Cardiovasc Surg, 2007. 55(3): p. 180-1. 9. J urgen P. A., et al., Bioglue: A Review of The Use of This New Surgical Adhesuve In Thoracic Surgery. ANZ Journal of Surgery, 2005. 75: p. 315-318. 10. Kit Wong, a.P.G., Effect of Fibrin Glue in the Reduction of Postthoracotomy Alveolar Air Leak. The Society of Thoracic Surgeons, 1997. 64: p. 979-981. 11. Murray, K.D., et al., The Influence of Pulmonary Staple Line Reinforcement on Air Leaks. Chest, 2002. 122(6): p. 2146-2149. 12. Nasim Annabi, e.a., Engineering a highly elastic human protein - based sealant for surgical applications. Science Translational Medcine, 2017. 9. 13. Kjaergard, H.K., et al., Prevention of Air Leakage by Spraying Vivostat Fibrin Sealant After Lung Resection in Pigs. Chest, 2000. 117(4): p. 1124-1127. 14. Thomas Fabian, M., et al., Fibrin Glue in Pulmonary Resection: A Prospective, Randomized, Blinded Study. The Society of Thoracic Surgeons, 2003. 75: p. 1587-1592. 15. Kosar, A., et al., The experimental use of N-butyl cyanoacrylate tissue adhesive in pulmonary wedge resections. Heart Lung Circ, 2012. 21(11): p. 711-4. 16. Klijian, A., A novel approach to control air leaks in comples lung surgery: a retrospective review. Journal of Cardiothoracic Surgery, 2012. 7(49). 17. Fuller, C., Reduction of intraoperative air leaks with Progel in pulmonary resection: a comprehensive review. Journal of Cardiothoracic Surgery, 2013 9(90). 18. Ibrahim, M., et al., Intraoperative bronchial stump air leak control by Progel(R) application after pulmonary lobectomy. Interact Cardiovasc Thorac Surg, 2016. 22(2): p. 222-4. 19. Despoudi, K., et al., Effects of albumin/glutaraldehyde glue on healing of colonic anastomosis in rats. World J Gastroenterol, 2017. 23(31): p. 5680-5691. 20. Spotnitz, W.D., Fibrin Sealant: The Only Approved Hemostat, Sealant, and Adhesive-a Laboratory and Clinical Perspective. ISRN Surg, 2014. 2014: p. 203943. 21. Anegg, U., et al., Efficiency of fleece-bound sealing (TachoSil) of air leaks in lung surgery: a prospective randomised trial. Eur J Cardiothorac Surg, 2007. 31(2): p. 198-202. 22. Mizrahi, B., C. Weldon, and D.S. Kohane, Tissue Adhesives as Active Implants. 2010. 8: p. 39-56. 23. Mehdizadeh, M. and J. Yang, Design strategies and applications of tissue bioadhesives. Macromol Biosci, 2013. 13(3): p. 271-88. 24. Petter-Puchner, A.H., et al., A comparison of a cyanoacrylate glue (Glubran) vs. fibrin sealant (Tisseel) in experimental models of partial pulmonary resection and lung incision in rabbits. J Invest Surg, 2010. 23(1): p. 40-7. 25. David F. Torchiana, M.D., Polyethylene Glycol Based Synthetic Sealants: Potential Uses in Cardiac Surgery. Journal of Cardiac Surgery 2003. 18: p. 504-506. 26. Hiroaki Nomori, M., et al., Gelatin-Resorcinol–Formaldehyde-Glutaraldehyde Glue for Sealing Pulmonary Air Leaks During Thoracoscopic Operation. The Society of Thoracic Surgeons, 1997. 67: p. 212-216. 27. Maximilian, B., et al., Albumin-glutaraldehyde glue for repair of superficial lung defect: an in vitro experiment. Journal of Cardiothoracic Surgery, 2016. 11(63). 28. Hung-Hsing C. and David, F.T., BioGlue: Albumin/Glutaraldehyde Sealant in Cardiac Surgery. Journal of Cardiac Surgery, 2003. 18: p. 500-503. 29. Kaushik, N.K., et al., Biomedical and Clinical Importance of Mussel-Inspired Polymers and Materials. Mar Drugs, 2015. 13(11): p. 6792-817. 30. Waite, J.H., Mussel adhesion - essential footwork. J Exp Biol, 2017. 220(Pt 4): p. 517-530. 31. Kord Forooshani, P. and B.P. Lee, Recent approaches in designing bioadhesive materials inspired by mussel adhesive protein. J Polym Sci A Polym Chem, 2017. 55(1): p. 9-33. 32. Kim, B.J., et al., Mussel-mimetic protein-based adhesive hydrogel. Biomacromolecules, 2014. 15(5): p. 1579-85. 33. Scognamiglio, F., et al., Enhanced bioadhesivity of dopamine-functionalized polysaccharidic membranes for general surgery applications. Acta Biomater, 2016. 44: p. 232-42. 34. Hou, J., et al., Enzymatically crosslinked alginate hydrogels with improved adhesion properties. Polymer Chemistry, 2015. 6(12): p. 2204-2213. 35. Bilic, G., et al., Injectable candidate sealants for fetal membrane repair: bonding and toxicity in vitro. Am J Obstet Gynecol, 2010. 202(1): p. 85 e1-9. 36. Zhang, H., et al., Mussel-inspired hyperbranched poly(amino ester) polymer as strong wet tissue adhesive. Biomaterials, 2014. 35(2): p. 711-9. 37. Bruce P. Lee, J.L.D., and Phillip B. Messersmith Synthesis and Gelation of DOPA-Modified Poly(ethylene glycol) Hydrogels. Biomacromolecules, 2002. 3: p. 1038-1047. 38. Wang, R., et al., A Biomimetic Mussel-Inspired ε-Poly-l-lysine Hydrogel with Robust Tissue-Anchor and Anti-Infection Capacity. Advanced Functional Materials, 2017. 27(8): p. 1604894. 39. Yang, J., M.A. Cohen Stuart, and M. Kamperman, Jack of all trades: versatile catechol crosslinking mechanisms. Chem Soc Rev, 2014. 43(24): p. 8271-98. 40. Rahimnejad, M. and W. Zhong, Mussel-inspired hydrogel tissue adhesives for wound closure. RSC Adv., 2017. 7(75): p. 47380-47396. 41. Koob, T.J., Biomimetic approaches to tendon repair. Comparative Biochemistry and Physiology Part A, 2002. 133: p. 1171-1192. 42. Fan, C., et al., A mussel-inspired double-crosslinked tissue adhesive intended for internal medical use. Acta Biomater, 2016. 33: p. 51-63. 43. Mehdizadeh, M., et al., Injectable citrate-based mussel-inspired tissue bioadhesives with high wet strength for sutureless wound closure. Biomaterials, 2012. 33(32): p. 7972-83. 44. Bohari, S.P., D.W. Hukins, and L.M. Grover, Effect of calcium alginate concentration on viability and proliferation of encapsulated fibroblasts. Biomed Mater Eng, 2011. 21(3): p. 159-70. 45. Barbetta, A., et al., Polysaccharide based scaffolds obtained by freezing the external phase of gas-in-liquid foams. Soft Matter, 2010. 6(20): p. 5213. 46. Fenn, S.L., P.N. Charron, and R.A. Oldinski, Anticancer Therapeutic Alginate-Based Tissue Sealants for Lung Repair. ACS Appl Mater Interfaces, 2017. 9(28): p. 23409-23419. 47. Christian J. Kastrupa, b., et al., Painting blood vessels and atherosclerotic plaques with an adhesive drug depot. PNAS, 2012. 109: p. 21444-21449. 48. Lee, C., et al., Bioinspired, calcium-free alginate hydrogels with tunable physical and mechanical properties and improved biocompatibility. Biomacromolecules, 2013. 14(6): p. 2004-13. 49. Zhang, S., et al., Mussel-inspired alginate gel promoting the osteogenic differentiation of mesenchymal stem cells and anti-infection. Mater Sci Eng C Mater Biol Appl, 2016. 69: p. 496-504. 50. Hyun-Joon Kong, K.Y.L., David J. Mooney, Decoupling the dependence of rheological/mechanical properties of hydrogels from solids concentration. Polymer Chemistry, 2002. 43: p. 6239-46. 51. Carlos Alberto Martínez-Huitlea, et al., Electrochemical Behaviour of Dopamine at Covalent Modified Glassy Carbon Electrode with L-Cysteine: Preliminary Results. Materials Research, 2009. 12(4): p. 375-384. 52. Cencer, M., et al., Effect of pH on the rate of curing and bioadhesive properties of dopamine functionalized poly(ethylene glycol) hydrogels. Biomacromolecules, 2014. 15(8): p. 2861-9. 53. Kawai, N., et al., Sealing Effect of Cross-Linked Gelatin Glue in the Rat Lung Air Leak Model. Ann Thorac Surg, 2016. 102(1): p. 282-6. 54. Mizuta, R. and T. Taguchi, Enhanced Sealing by Hydrophobic Modification of Alaska Pollock-Derived Gelatin-Based Surgical Sealants for the Treatment of Pulmonary Air Leaks. Macromol Biosci, 2017. 17(4). 55. K.Vijay, D.S.a.I., A Method for the High Efficiency of Water-Soluble Carbodiimide-Mediated Amidation. Anayltical, Biochemistry, 1994. ,218: p. 87-91 56. Wang, X., et al., Dopamine-Modified Alginate Beads Reinforced by Cross-Linking via Titanium Coordination or Self-Polymerization and Its Application in Enzyme Immobilization. Industrial & Engineering Chemistry Research, 2013. 52(42): p. 14828-14836. 57. Alegre-Requena, J.V., et al., Regulatory parameters of self-healing alginate hydrogel networks prepared via mussel-inspired dynamic chemistry. New J. Chem., 2016. 40(10): p. 8493-8501. 58. Meng, H., et al., Hydrogen peroxide generation and biocompatibility of hydrogel-bound mussel adhesive moiety. Acta Biomater, 2015. 17: p. 160-9. 59. Meng, H., Y. Liu, and B.P. Lee, Model polymer system for investigating the generation of hydrogen peroxide and its biological responses during the crosslinking of mussel adhesive moiety. Acta Biomater, 2017. 48: p. 144-156. 60. Marco, R.G.P., et al., Cytotoxicity of catechol towards human glioblastoma cells via superoxide and reactive quinones generation. Jornal Brasileiro de Patologia e Medicina Laboratorial, 2004. 4: p. 280-285. 61. Marie-Veronique Clement, et al., The cytotoxicity of dopamine may be an artefact of cell culture. Journal of Neurochemistry, 2002. 81: p. 414–421. 62. Feng, J., et al., Mechanically Reinforced Catechol-Containing Hydrogels with Improved Tissue Gluing Performance. Biomimetics, 2017. 2(4): p. 23. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79168 | - |
dc.description.abstract | 肺臟切除術是針對肺臟腫瘤、肺囊腫或是感染性疾病所使用的治療方式,切除後的肺葉或氣管會以自動吻合器進行術後釘合。諸多併發症中,以肺臟持續性漏氣最為常見,可能引發肺部感染、膿胸或是引流時間延長等問題。為減少漏氣現象,現今會使用組織膠或支撐材來補強釘腳孔隙或是癒合不完全形成的傷口。然而,這些商品價格普遍昂貴,且部分具細胞毒性或黏性不足等問題。因此我們合成出一種新材料,希望能降低以上情況並達到減少肺漏氣的效果。材料的設計引自仿生的概念,以褐藻酸鈉做為基底合成一種可注射式水膠,作為新的組織膠。近年研究發現,貽貝類的觸手可於含水環境中有效黏著於有機與無機物表面,原因來自於其成分中大量的鄰苯二酚(catechol)。這種官能基能與不同表面鍵結產生共價鍵及非共價鍵,提供高度的黏性。因此,我們透過EDC/NHS反應將多巴胺(dopamine),接枝在高分子褐藻酸鈉上,形成鄰苯二酚-褐藻酸鈉(catechol-alginate)。將此材料溶成水溶液,與不同濃度高碘酸鈉混和,使之於短時間內形成高黏著性的水膠。透過成膠時間、組織拉伸、爆破壓力、機械性質以及細胞活性等測試,確認我們合成的材料,確實可有效在肺部傷口外形成保護層,並且不具明顯細胞毒性。以現階段實驗結果發現,以2%鄰苯二酚-褐藻酸鈉,與高碘酸鈉離子對鄰苯二酚莫爾數比為0.75的高碘酸鈉溶液形成之水膠,能提供組織最佳的黏著力,並且負荷285.7 ± 36.0 mmH2O的肺部壓力。 | zh_TW |
dc.description.abstract | Prolonged air leak is one of the most common complications after lung parenchyma resection. To deal with the problem, surgeons might use tissue adhesives or buttressing material to wrap around the anastomosis of lung incision after stapling. However, the commercial bioadhesives have drawbacks such as low adhesive properties and cytotoxicity. Hence, we conceived an idea of using mussel protein-based material to make a potential bioadhesive since mussel exhibits strong bulk wet tissue adhesion due to the function of abundant catechol groups in their byssus. In this paper, we conjugated dopamine, a small molecule with catechol group, onto the backbone of sodium alginate to produce catechol-alginate; and then successfully fabricated adhesive hydrogel in the presence of sodium periodate (NaIO4). To investigate the effect of this adhesive, several methods were used –vial tilting assay, cell viability assay, mechanical properties, lap shear stress test and ex vivo test of bursting pressure. Based on the results, 2% C-Alg and NaIO4 with IO4/catechol ratio 0.75 was found as optimum, having the best adhesive properties and enabling a bursting pressure of rat lung to 285.7 ± 36.0 mmH2O. | en |
dc.description.provenance | Made available in DSpace on 2021-07-11T15:49:42Z (GMT). No. of bitstreams: 1 ntu-107-R05548007-1.pdf: 2920841 bytes, checksum: 0de4eab13b22cf30420f504c19fa56d9 (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 誌謝 i
中文摘要 ii ABSTRACT iii CONTENTS iv LIST OF FIGURES vi LIST OF TABLES vii Chapter 1 Introduction 1 1.1 Pulmonary Air Leaks 1 1.1.1 Lung parenchyma resection 1 1.1.2 ProlongedAirLeaks (PAL) 1 1.2 Tissue adhesive used on pulmonary resection 2 1.2.1 Fibringlues (FG) 3 1.2.2 Cyanoacrylate adhesives (CA) 4 1.2.3 Polyethylene glycol (PEG) adhesives 5 1.2.4 Protein based adhesive 7 1.3 Mussel-inspired adhesive 8 1.3.1 Catechol chemistry 10 1.3.2 Catechol-based tissue adhesives 12 1.3.3 Sodium Alginate 13 1.3.4 Catechol-conjugated Alginate 14 1.4 Objectives 15 Chapter 2 Materials and methods 16 2.1 Experimental Structure 16 2.2 Materials 17 2.3 Equipment 18 2.4 Methods 19 2.4.1 Synthesis of catechol-modified alginates (C-Alg) 19 2.4.2 Characterization methods 19 2.4.3 Adhesive hydrogel formation 20 2.4.4 Gelation time 20 2.4.5 Cell culture 21 2.4.6 Cell viability 21 2.4.7 Mechanical characteristics 22 2.4.8 Degradation 23 2.4.9 Lap shear stress 23 2.4.10 Bursting pressure 24 2.5 Statistical Analysis 25 Chapter 3 Results and Discussion 26 3.1 Synthesis of C-Alg 26 3.2 The Substitution degree (SD) 27 3.3 Gelation time 28 3.4 Cell viability 29 3.5 Mechanical characteristics 32 3.6 Degradation 33 3.7 Lap shear stress 33 3.8 Bursting Pressure 34 Chapter 4 Conclusion 36 References 49 | |
dc.language.iso | en | |
dc.title | 以鄰苯二酚接枝褐藻酸鈉水膠減少肺漏氣之效果 | zh_TW |
dc.title | Effects of reducing pulmonary air leaks by catechol-modified sodium alginate hydrogel | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蔡偉博(Wei-bor Tsai),陳克誠(Ke-Cheng Chen) | |
dc.subject.keyword | 褐藻酸鈉,鄰苯二酚,組織膠,肺漏氣,高碘酸鈉, | zh_TW |
dc.subject.keyword | sodium alginate,catechol,bioadhesive,air leak,sodium periodate, | en |
dc.relation.page | 53 | |
dc.identifier.doi | 10.6342/NTU201802061 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-07-31 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
dc.date.embargo-lift | 2023-08-02 | - |
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