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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 楊台鴻(Tai-Horng Young) | |
| dc.contributor.author | Sheng-Hao Tseng | en |
| dc.contributor.author | 曾盛豪 | zh_TW |
| dc.date.accessioned | 2021-07-11T15:47:25Z | - |
| dc.date.available | 2023-08-06 | |
| dc.date.copyright | 2018-08-06 | |
| dc.date.issued | 2018 | |
| dc.date.submitted | 2018-08-03 | |
| dc.identifier.citation | 1. Oliveira, Joaquim Miguel., and Reis Rui Luís. Regenerative Strategies for the Treatment of Knee Joint Disabilities. Springer International Publishing, 2017.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79142 | - |
| dc.description.abstract | 臨床上前十字韌帶重建手術雖可以由病人自體之髕骨肌腱或腿後肌肌腱，取代受傷之韌帶重建韌帶組織，但由於缺乏血管移植的肌腱組織在低氧和低營養的環境，延長了移植組織和肌腱-骨頭間(tendon-bone)癒合的修復時間，而有移植失敗之風險。因此，若能在手術過程中搭配有效的促進療法，必能提高重建手術的效果。
血小板濃厚液(platelet-rich plasma, PRP)富含生長因子，已被大量使用於退化性關節炎之病患，應可作為加速前十字韌帶重建時之韌帶癒合、tendon-bone修復的方法。但流體狀的PRP注入於患部時，會流散開來無法停滯於患部，因此，以生醫材料作為PRP包覆之載體，使PRP包覆於顆粒內，除減緩其流動性，能有助於緩慢釋出PRP，使PRP能在患部有較長時間釋出生長因子，達到較佳的治療效果。 故本研究探討生醫材料結合PRP後，前十字韌帶纖維母細胞於低血清與低氧氣環境中的活性( cell viability )、增生( proliferation )及遷移( migration )能力，並建立兔子的tendon-bonem癒合模型，進行PRP微粒在體外和體內實驗的評估。 | zh_TW |
| dc.description.abstract | Clinical anterior cruciate ligament reconstruction surgery often use autologous patellar tendon or hamstring (semitendinosus) graft to replace the ruptured ligament. However, since the transplanted tissue does not have an intact blood supply and is in low oxygen and nutrient environment, the graft and tendon-bone healing time will be extended with a high risk of failed surgery. Therefore, the purpose of the project is to provide a medical innovation combined with biomaterial and platelet-rich plasma (PRP) to improve the result of reconstructive surgery.
PRP, rich in growth factors, has been extensively used in patients with degenerative arthritis so it is available as a tool to enhance ligament and tendon-bone healing at ligament reconstruction surgery. To control the fluid-like property of PRP, which is not able to stagnate in the affected part, this study will encapsulate PRP by an appropriate biomaterial to sluggish its fluidity and to control growth factors release from PRP beads. It is anticipated that PRP can be maintained in the affected part for a longer time and the better therapeutic effect is achieved. Therefore, this study explored the cell viability、proliferation ability and migration ability of the anterior cruciate ligament cells by combining biomaterials and PRP in low serum and low oxygen environment and to establish rabbit ligament healing model. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-11T15:47:25Z (GMT). No. of bitstreams: 1 ntu-107-R05548006-1.pdf: 4618362 bytes, checksum: de2901085a8d0dfc7a559df9af6c18e1 (MD5) Previous issue date: 2018 | en |
| dc.description.tableofcontents | 口試委員會審定書 #
誌謝 i 中文摘要 ii 英文摘要 iii 目錄 iv 圖目錄 vii 表目錄 ix 第一章 文獻回顧 1 1.1 膝關節 ( knee joint )……………………… 1 1.2 前十字韌帶 ( anterior cruciate ligament, ACL ) 2 1.2.1 前十字韌帶損傷 ( ACL injury ) 3 1.2.2 前十字韌帶重建術 ( ACL reconstruction ) 4 1.2.3 前十字韌帶復原情況 ( ACL rehabilitation ) 5 1.3 血小板生物學 ( platelet biology ) 6 1.3.1 血小板功能 ( platelet functions ) 6 1.3.2 血小板活化 ( platelet activation ) 8 1.3.3 血小板中的生長因子 ( growth factors present in platelet ) 9 1.4 血小板濃厚液 ( platelet - rich plasma, PRP ) 11 1.4.1 血小板濃厚液製備 ( PRP preparation ) 11 1.4.2 血小板濃厚液應用 ( PRP applications ) 13 1.5 褐藻酸 ( alginate ) 14 1.6 研究動機 15 第二章 實驗材料與方法 16 2.1 實驗架構……………………………. 16 2.2 實驗材料 18 2.2.1 化學藥品 18 2.2.2 細胞培養 19 2.2.3 實驗儀器 19 2.3 試劑、藥品製備 21 2.4 實驗方法 23 2.4.1 血小板濃縮液製備與鑑定 23 2.4.2 微粒製作 24 2.4.3 膨潤度測試 24 2.4.4 蛋白釋放測試 25 2.4.5 細胞分離和培養 26 2.4.6 MTT assay 26 2.4.7 細胞增生實驗 27 2.4.8 細胞遷移實驗 28 2.4.9 統計分析 29 第三章 實驗結果與討論 30 3.1 血小板濃縮效益 30 3.2 微粒膨潤度 30 3.3 蛋白釋放量 31 3.4 前十字韌帶纖維母細胞 31 3.5 ACLs於PRP培養下的細胞型態 31 3.6 ACLs於PRP培養下的細胞活性 31 3.7 ACLs於PRP培養下的細胞增生情形 31 3.8 ACLs於PRP培養下的細胞遷移情形 35 第四章 結論 37 圖 38 表 47 文獻回顧 48 | |
| dc.language.iso | zh-TW | |
| dc.subject | 肌腱-骨介面癒合 | zh_TW |
| dc.subject | 血小板濃厚液 | zh_TW |
| dc.subject | 生長因子 | zh_TW |
| dc.subject | 生醫材料 | zh_TW |
| dc.subject | 控制釋放 | zh_TW |
| dc.subject | growth factors | en |
| dc.subject | tendon-bone healing | en |
| dc.subject | controlled release | en |
| dc.subject | biomaterials | en |
| dc.subject | PRP | en |
| dc.title | 以交聯型生醫材料開發緩釋血小板濃縮液之醫材促進肌腱韌帶修補組織介面之癒合 | zh_TW |
| dc.title | Development and evaluation of cross-linked PRP-loaded granules for sustained-release in the healing of orthopedic tissue interfaces by repairing tendons and ligaments | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 106-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 王至弘(Jyh-Horng Wang) | |
| dc.contributor.oralexamcommittee | 李亦宸(Yi-Chen Li) | |
| dc.subject.keyword | 血小板濃厚液,生長因子,生醫材料,控制釋放,肌腱-骨介面癒合, | zh_TW |
| dc.subject.keyword | PRP,growth factors,biomaterials,controlled release,tendon-bone healing, | en |
| dc.relation.page | 52 | |
| dc.identifier.doi | 10.6342/NTU201802338 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2018-08-03 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
| dc.date.embargo-lift | 2023-08-06 | - |
| 顯示於系所單位： | 醫學工程學研究所 | |
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