請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79125
標題: | 開發平行化奈米預濃縮技術整合奈米狹縫表面電漿共振晶片用於免標定抗體抗原反應之研究 Development of Parallel Nanofluidic Preconcentrator and Nanoslit Surface Plasmon Resonance Sensor for Immunoassay |
作者: | Wun-Chang Kuo 郭文昌 |
指導教授: | 沈弘俊(Horn-Jiunn Sheen) |
關鍵字: | 深反應離子蝕刻,奈米壓印技術,電驅動奈米流體預濃縮,週期性奈米金屬表面電漿共振,生物感測器,免標定免疫分析,實驗室晶片, Parallel Nanofluidic Preconcentration,Deep Reactive Ion Etching,Nanoimprint Lithography,Nanoslit-based Surface Plasmon Resonance,Lab on a chip, |
出版年 : | 2018 |
學位: | 碩士 |
摘要: | 本研究之目的為開發新型免疫分析檢測裝置,欲整合週期性奈米金屬表面電漿共振(Periodic Metallic Surface Plasmon Resonance)感測器與平行化奈米流體預濃縮機制(Nanofluidic Preconcentration Mechanism)在一塑膠基板上。將待測活性分子在平行多流道中同時進行預濃縮,再藉由調整電位差來控制濃縮區塊,使其移動於各流道中的表面電漿共振晶片上方,最後量測其光譜訊號紅移(Redshift)以進行後續免疫分析,達到多流道同時濃縮並進行檢測的效果。
本研究係利用電子束微影(E-beam Lithography)和反應離子蝕刻(Reactive Ion Etching, RIE)在矽晶圓基材上定義出週期性奈米狹縫結構,接著以此結構製作出可用於自動壓印的鎳鈷材質沖壓模具,之後以PC(Polycarbonate)塑膠模作為晶片基板材料,利用自動化熱壓印將鎳鈷材質模具上之結構轉印至此高分子聚合物上,並經由遮罩濺鍍完成局部鍍金,再使用奈米多孔性材料Nafion®作為奈米流道鋪設於表面電漿共振晶片旁邊,確立奈米預濃縮結構。最後使用由傳統黃光微影製程和軟微影(Soft Lithography)製程製作以聚二甲基矽氧烷(Polydimethylsiloxane, PDMS) 為材料的微流道。經由化學表面修飾及氧電漿表面改質接合PC基板與PDMS微流道,即完成本免標定免疫分析晶片。 本研究檢測之樣本為人體免疫球蛋白(Immunoglobulin,Ig),實驗時,先各別將Anti-IgA、Anti-IgG以及Anti-IgM修飾於奈米狹縫晶片上,接著將IgA、IgG以及IgM通入於濃縮流道內進行濃縮,藉由操控電壓使濃縮區塊限定於奈米狹縫晶片上,接著比對濃縮前後的穿透特性光譜,經由紅移量的差異來驗證濃縮效果。 自動化奈米壓印技術成功達到量產(Mass Production)、低材料成本(Low-cost)和低時間成本的快速製程,且奈米流體預濃縮降低了檢測下限,加上表面電漿共振具有高靈敏度、即時性、和免標定的優勢,我們藉由簡便的量測系統完成了一個快速、免標定且微量的檢測平台。 The purpose of this study was to integrate parallel nanofluidic preconcentrator with periodic metallic surface plasmon resonance sensor in an immunoassay platform. The concentrated protein was trapped in the sensing area of SPR in a microfluidic channel by electrical potential difference. At last, measured the spectral signal red-shift of antibody-antigen interaction on periodic metallic slits for subsequent immunoassay. The periodic nano-grating structure were clarified and fabricated on a silicon wafer by E-beam lithography and reactive ion etching, then the structure was fabricated on a cobalt, nickel based metal stamping die by foundry. The nanostructure was transferred onto a polycarbonate (PC) by automated hot stamping. The gold was deposited on the grating structure of PC by sputter. A porous material, Nafion, used as the ion-selective channel, was aligned to the grating structure on PC. Microchannel were made by polydimethylsiloxane (PDMS) using soft lithography process. After the chemically modified surface treatment, the PC was bond with PDMS by oxygen plasma. The metallic SPR sensor was located in microfluidic channel, which were located cross over the Nafion channel. In this study, Immunoglobulins (Ig) were used as the testing samples. Immunoglobulin A, immunoglobulin G and immunoglobulin M was introduced into the microchannel. Then, by adjusting electrical potential, the antibodies were condensed on the surface plasmon resonance chip. The concentrated magnification can be sufficiently derived by the red-shifted value deference and the reference curves of immunoglobulins concentration. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79125 |
DOI: | 10.6342/NTU201802580 |
全文授權: | 有償授權 |
電子全文公開日期: | 2023-08-13 |
顯示於系所單位: | 應用力學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-107-R05543037-1.pdf 目前未授權公開取用 | 5.08 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。