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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 李水盛(Shoei-Sheng Lee) | |
dc.contributor.author | Tsun-Yu Lu | en |
dc.contributor.author | 呂尊昱 | zh_TW |
dc.date.accessioned | 2021-07-11T15:14:49Z | - |
dc.date.available | 2022-08-28 | |
dc.date.copyright | 2019-08-28 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-07-29 | |
dc.identifier.citation | 1. Yuen, P. Y.; Bing, L. S.; Huang, Y. L. A revision of Elatostema (Urticaceae of Taiwan). Botanical Bulletin of Academia Sinica 1995, 36, 259-279.
2. Monro, A. K. The revision of species-rich genera: a phylogenetic framework for the strategic revision of Pilea (Urticaceae) based on cpDNA, nrDNA, and morphology. American Journal of Botany 2006, 93, 426-441. 3. Liu, Y. P.; Bing, L. S.; Hong, Y. Flora of Taiwan, second edition. Taiwan, Endemic species research institute 1996. 4. Liu, S. T. Flora of Taiwan, first edition. Taiwan, Endemic species research institute 1976. 5. Chun, W. Y.; Tsung, S. C. Flora of China. China, China science 1994. 6. Miyazawa, M.; Utsumi, Y.; Kawata, J. Aroma-active compounds of Elatostema laetevirens and Elatostema umbellatum var. majus. Journal of Oleo Science 2009, 58, 163-169. 7. Liao, C. S.; Tang, C. P.; Yao, S.; Ye, Y. Humulane-type sesquiterpenoids from Pilea cavaleriei subsp. crenata. Organic & Biomolecular Chemistry 2013, 11, 4840-4846. 8. Tang, G. H.; Sun, C. S.; Long, C. L.; Li, M. L.; Wang, Y. H.; Luo, M.; Wang, H. S.; Shi, Y. N. Sesquiterpenoids from Pilea cavaleriei subsp. crenata. Bioorganic & Medicinal Chemical Letters 2009, 19, 5737-5740. 9. Zhou, Y.; Li, L. Y.; Ren, H. C.; Qin, R. D.; Li, Q.; Tu, P. F.; Dou, G. F.; Zhang, Q. Y.; Liang, H. Chemical constituents from the whole plants of Pilea cavaleriei Levi. subsp. cavaleriei. Fitoterapia 2017, 119, 100-107. 10. Yan, H.; Xie, W.; Jian, W. W.; Ming, S. C.; Kai, J. H.; Bing, M. L. Chemical constituents of Pilea sinofasciata. Chinese Traditional and Herbal Drugs 2016, 47, 3159-3163. 11. Bansal, P.; Paul, P.; Nayak, P. G.; Pannakal, S. T.; Zou, J. H.; Laatsch, H.; Priyadarsini, K. I.; Unnikrishnan, M. K. Phenolic compounds isolated from Pilea microphylla prevent radiation-induced cellular DNA damage. Acta Pharmaceutica Sinica B 2011, 1, 226-235. 12. Chakravarty, A. K.; Das, B.; Masuda, K.; Ageta, H. Glycoric acid possessing a new 10-normegastigmane skeleton from Glycosmis arborea. Chemical and Pharmaceutical Bulletin 1996, 44, 1421-1423. 13. Hou, Y. Z.; Chen, K. K.; Deng, X. L.; Fu, Z. L.; Chen, D. F.; Wang, Q. Anti-complementary constituents of Anchusa italica. Natural Product Research 2017, 31, 2572-2574. 14. Erdelmeier, C. A. J.; Sticher, O. A cyclohexenone and a cyclohexadienone glycoside from Eryngium campestre. Phytochemistry 1986, 25, 741-743. 15. Major Jourden, J. L.; Cohen, S. M. Enzymatic activation of a matrix metalloproteinase inhibitor. Chemical Communications (Cambridge) 2010, 46, 1241-1243. 16. Sala, A.; Recio, M. C.; Schinella, G. R.; Manez, S.; Giner, R. M.; Rios, J. L. A new dual inhibitor of arachidonate metabolism isolated from Helichrysum italicum. European Journal Pharmacology 2003, 460, 219-226. 17. Jin, Y. P.; Wei, X. N.; Shi, Y. P. Chemical constituents from Cynoglossum gansuense. Helvetica Chimica Acta 2007, 90, 776-782. 18. Kolak, U.; Ari, S.; Birman, H.; Hasancebi, S.; Ulubelen, A. Cardioactive diterpenoids from the roots of Salvia amplexicaulis. Planta Medica 2001, 67, 761-763. 19. De Kimpe, N.; Sulmon, P.; Brunet, P.; Lambein, F.; Schamp, N. Synthesis of 2,2-dialkyl-L-aminocyclopropanecarboxylic acids from α-chloroimines. Tetrahedron Letters 1989, 30, 1863-1866. 20. Kim, C. S.; Subedi, L.; Kwon, O. W.; Park, H. B.; Kim, S. Y.; Choi, S. U.; Lee, K. R. Wasabisides A-E, lignan glycosides from the roots of Wasabia japonica. Journal of Natural Product 2016, 79, 2652-2657. 21. Sang, S. M.; Kikuzaki, H.; Lapsley, K.; Rosen, R. T.; Nakatani, N.; Ho, C. T. Sphingolipid and other constituents from almond nuts (Prunus amygdalus Batsch). Journal of Agricultural and Food Chemistry 2002, 50, 4709-4712. 22. Kitajima, J.; Ishikawa, T.; Tanaka, T.; Ida, Y. Water-soluble constitutents of fennel IX. glucides and nucleosides. Chemical and Pharmaceutical Bulletin 1999, 47, 988-992. 23. Hooper, A. M.; Caulfield, J. C.; Hao, B.; Pickett, J. A.; Midega, C. A. O.; Khan, Z. R. Isolation and identification of Desmodium root exudates from drought tolerant species used as intercrops against Striga hermonthica. Phytochemistry 2015, 117, 380-387. 24. Baruah, T. J.; Sharan, R. N.; Kma, L. Vicenin-2: a potential radiosensitizer of non-small cell lung cancer cells. Molecular Biology Reports 2018, 45, 1219-1225. 25. Yang, D.; Zhang, X.; Zhang, W.; Rengarajan, T. Vicenin-2 inhibits Wnt/beta-catenin signaling and induces apoptosis in HT-29 human colon cancer cell line. Drug Design Develop and Therapy 2018, 12, 1303-1310. 26. Marrassini, C.; Davicino, R.; Acevedo, C.; Anesini, C.; Gorzalczany, S.; Ferraro, G. Vicenin-2, a potential anti-inflammatory constituent of Urtica circularis. Journal of Natural Product 2011, 74, 1503-1507. 27. Casanova, L. M.; Gu, W.; Costa, S. S.; Jeppesen, P. B. Phenolic substances from Ocimum species enhance glucose-stimulated insulin secretion and modulate the expression of key insulin regulatory genes in mice pancreatic islets. Journal of Natural Product 2017, 80, 3267-3275. 28. Hooper, A. M.; Tsanuo, M. K.; Chamberlain, K.; Tittcomb, K.; Scholes, J.; Hassanali, A.; Khan, Z. R.; Pickett, J. A. Isoschaftoside, a C-glycosylflavonoid from Desmodium uncinatum root exudate, is an allelochemical against the development of Striga. Phytochemistry 2010, 71, 904-908. 29. Du, S. S.; Zhang, H. M.; Bai, C. Q.; Wang, C. F.; Liu, Q. Z.; Liu, Z. L.; Wang, Y. Y.; Deng, Z. W. Nematocidal flavone-C-glycosides against the root-knot nematode (Meloidogyne incognita) from Arisaema erubescens tubers. Molecules 2011, 16, 5079-5086. 30. de Oliveira, A. P.; Coppede, J. S.; Bertoni, B. W.; Crotti, A. E. M.; Franca, S. C.; Pereira, A. M. S.; Taleb-Contini, S. H. Costus spiralis (Jacq.) Roscoe: a novel source of flavones with alpha-glycosidase inhibitory activity. Chemical Biodivers 2018, 15, 296-300. 31. Xie, C.; Veitch, N. C.; Houghton, P. J.; Simmonds, M. S. J. Flavone C-glycosides from Viola yedoensis Makino. Chemical and Pharmaceutical Bulletin 2003, 51, 1204-1207. 32. Zhou, K.; Wu, J.; Chen, J.; Zhou, Y.; Chen, X.; Wu, Q.; Xu, Y.; Tu, W.; Lou, X.; Yang, G.; Jiang, S. Schaftoside ameliorates oxygen glucose deprivation-induced inflammation associated with the TLR4/Myd88/Drp1-related mitochondrial fission in BV2 microglia cells. Journal of Pharmacological Science 2019, 139, 15-22. 33. Kim, P. S.; Shin, J. H.; Jo, D. S.; Shin, D. W.; Choi, D. H.; Kim, W. J.; Park, K.; Kim, J. K.; Joo, C. G.; Lee, J. S.; Choi, Y.; Shin, Y. W.; Shin, J. J.; Jeon, H. B.; Seo, J. H.; Cho, D. H. Anti-melanogenic activity of schaftoside in Rhizoma Arisaematis by increasing autophagy in B16F1 cells. Biochemical and Biophysical Research Communications 2018, 503, 309-315. 34. Luyen, B. T.; Tai, B. H.; Thao, N. P.; Cha, J. Y.; Lee, H. Y.; Lee, Y. M.; Kim, Y. H. Anti-inflammatory components of Chrysanthemum indicum flowers. Bioorganic & Medicinal Chemical Letters 2015, 25, 266-269. 35. Wang, X.; Yuan, T.; Yin, N.; Ma, X.; Zhang, Z.; Zhu, Z.; Shaukat, A.; Deng, G. Luteoloside protects the uterus from Staphylococcus aureus-induced inflammation, apoptosis, and injury. Inflammation 2018, 41, 1702-1716. 36. Nagy, M.; Krizkova, L.; Mucaji, P.; Kontsekova, Z.; Sersen, F.; Krajcovic, J. Antimutagenic activity and radical scavenging activity of water infusions and phenolics from Ligustrum plants leaves. Molecules 2009, 14, 509-518. 37. Besson, E.; Chopin, J.; Markham, K. R.; Mues, R.; Wong, H.; Bouillant, M. L. Identification of neoschaftoside as 6-C-β-D-glucopyranosyl-8-C-β-L-arabinopyranosylapigenin. Phytochemistry 1984, 23, 159-161. 38. Schroder, D.; Budesinsky, M.; Roithova, J. Deprotonation of p-hydroxybenzoic acid: does electrospray ionization sample solution or gas-phase structures? Journal of the American Chemical Society 2012, 134, 15897-15905. 39. Martin, T. S.; Kikuzaki, H.; Hisamoto, M.; Nakatani, N. Constituents of Amomum tsao-ko and their radical scavenging and antioxidant activities. Journal of the American Oil Chemists' Society 2000, 77, 667-673. 40. Hong, S. S.; Choi, C. W.; Choi, Y. H.; Oh, J. S. Coixlachryside A: a new lignan glycoside from the roots of Coix lachryma-jobi L. var. ma-yuen Stapf. Phytochemistry Letters 2016, 17, 152-157. 41. Thompson, J.; Robrish, S. A.; Pikis, A.; Brust, A.; Lichtenthaler, F. W. Phosphorylation and metabolism of sucrose and its five linkage-isomeric alpha-D-glucosyl-D-fructoses by Klebsiella pneumoniae. Carbohydrate Research 2001, 331, 149-161. 42. Lampert, H.; Mikenda, W.; Karpfen, A.; Kahlig, H. NMR shieldings in benzoyl and 2-hydroxybenzoyl compounds. Experimental versus GIAO calculated data. Journal of Physical Chemistry A 1997, 101, 9610-9617. 43. Lavoie, S.; Legault, J.; Simard, F.; Chiasson, E.; Pichette, A. New antibacterial dihydrochalcone derivatives from buds of Populus balsamifera. Tetrahedron Letters 2013, 54, 1631-1633. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78724 | - |
dc.description.abstract | 狹葉赤車使者Elatostema lineolatum Wight var. majus Wedd. (Urticaceae)為蕁麻科樓梯草屬之多年生草本植物,分佈於日本、中國及台灣中低海拔之潮濕山區。經由文獻查詢,目前尚無相關成分之研究。而由於同屬植物之低極性成分已有報告,本研究遂著重於中高極性成分之探討,以窺探其潛在的醫療用途。利用Sephadex LH-20管柱、離心分配層析、矽膠管柱、低壓逆相層析管柱及半製備高壓液相層析管柱進行分離,自本植物地上部乙醇萃取物之乙酸乙酯與正丁醇可溶二部分分別分離並鑑定出6個(1~6)及10個(7~16)化合物。
大冷水麻Pilea melastomoides (Poir.) Wedd. (Urticaceae)為蕁麻科冷水麻屬之多年生草本植物,分佈於印度、中國及台灣中低海拔之闊葉林區。本部分主要研究該植物地上部之中高極性成分,探討其潛在的醫療用途。大冷水麻之乙醇萃取物以極性劃分為2%乙酸可溶與不可溶二部分,2%乙酸可溶部分進一步以乙酸乙酯和水進行分配,再劃分成乙酸乙酯和水可溶二部分,其中水可溶部分利用氨水鹼化後再以三氯甲烷抽提,得三氯甲烷可溶部分。水層以XAD-II管柱層析後,得到甲醇沖提物。經由各種層析,分別自乙酸乙酯可溶部分、甲醇沖提物、酸不可溶部分分離並鑑定出2個(17 and 18)、4個(11, 12, 19 and 20)及1個(21)化合物。 上述成分之結構主要係藉由解析核磁共振譜(氫、碳-13與二維圖譜)並搭配質譜資料而得到。其中化合物2為新化合物。化合物1~16, 19, 20及21首次自同屬植物中分離得到。且Wasabiside (3)具有神經保護的作用;Vicenin-2 (13)可促進胰島素分泌;Maltol glucoside (19)具有抑制發炎之生物活性;Stigmast-4-en-3-one (21)於大鼠試驗中具明顯降低血壓之效果。 | zh_TW |
dc.description.abstract | Elatostema lineolatum Wight var. majus Wedd. (Urticaceae) is a perennial herbaceous plant distributed in the humid forests from low to medium altitudes in Japan, China and Taiwan. As its chemical constituents of low polarity have been reported and its potential medical use remains explored, its chemical constituents of moderate and higher polarity were investigated in this study. The 95% ethanol extract of the aerial part of this plant was divided into fractions soluble in CHCl3, EtOAc, n-BuOH, and H2O via liquid-liquid partitioning process. EtOAc and n-BuOH soluble fractions were separated by Sephadex LH-20 column, cetrifugul partition chromatography, silica gel column, ODS reverse phase column , semi-preperative RP-18 HPLC. These efforts led to isolation of six compounds (1~6) from the EtOAc-soluble fraction and ten compounds (7~16) from the n-BuOH-soluble fraction.
Pilea melastomoides (Poir.) Wedd. (Urticaceae) is a perennial herbaceous plant, distributed in the broad-leaved forests from low to medium altitudes in India, China and Taiwan. As this plant is quite abundant in mountain area of northern Taiwan and its potential medical use remains explored, its chemical constituents were investigated in this study. The 95% ethanol extract of the aerial part of this plant was divided into 2% acetic acid-soluble fraction and residue. Acid-soluble part was extracted by EtOAc to give an EtOAc-soluble fraction. The aqueous fraction was basified by ammonia water and then extracted by CHCl3 to give CHCl3-soluble fraction. The aqueous layer was then passed through XAD-II column to give a MeOH-eluted fraction. By various chromatographic methods, two compounds (17 and 18) were isolated and identified from the EtOAc-soluble fraction, four compounds (11, 12, 19 and 20) from the MeOH-eluted fraction, and one compound (21) from the acid-insoluble residue. The strutures of these isolated compounds were elucidated mainly by spectroscopic analysis of 1D and 2D NMR assisted by MS data. Among them compound 2 is new, and compound 1~16, were first found in the Elatostema plants; compound 19, 20 and 21 were first found in the Pilea plants. Amomg these compounds, compound 3 can protect neurons, compound 13 can increase insulin level; compound 19 has anti-inflammatory activity; compound 21 can decrease blood pressure in rats. | en |
dc.description.provenance | Made available in DSpace on 2021-07-11T15:14:49Z (GMT). No. of bitstreams: 1 ntu-108-R06423004-1.pdf: 8135108 bytes, checksum: 1c6b99d1293aa66bff30c29dc6d9884a (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 中文摘要 I
Abstract II 總目錄 III 流程圖目錄(List of Schemes) VI 表目錄(List of Tables) VII 圖目錄(List of Figures) IX 專有名詞縮寫(Abbreviatoin of Terminology) XIII 壹、植物簡介與研究目的 1 1.1植物簡介 1 1.2 研究目的 3 貳、實驗結果與討論 16 2.1 狹葉赤車使者及大冷水麻地上部成分之結構解析 18 2.1.1 萜類 (1、8、9、19 and 21) 18 2.1.1.1 (7E,3R,5S,6R)-3,6-Dihydroxy-10-normegastigm-7-en-9-oic acid (1) 18 2.1.1.2 Citroside B (8) 20 2.1.1.3 3-(β-D-Glucopyranosyloxymethyl)-2,4,4-trimethyl-2-cyclohexen-1-one (9) 24 2.1.1.4 Maltol glucoside (19) 26 2.1.1.5 Stigmast-4-en-3-one (21) 28 2.1.2 胺基酸衍生物類 (2) 30 2.1.2.1 Elalinine (2) 30 2.1.3 木脂苷類 34 2.1.3.1 Wasabiside A (3) 34 2.1.4 核苷類 (11 and 12) 38 2.1.4.1 Uridine (11) 38 2.1.4.2 Uracil (12) 39 2.1.5 黃酮苷類 (13~16、18 and 20) 40 2.1.5.1 Vicenin-2 (13) 40 2.1.5.2 Isoschaftoside (14) 42 2.1.5.3 Schaftoside (15) 45 2.1.5.4 Apigenin 6,8-di-C-α-L-arabinopyranoside (16) 49 2.1.5.5 Luteolin 7-O-glucoside (18) 51 2.1.5.6 Luteolin 7-O-rutinoside (20) 53 2.1.6 其它化合物 (4~7、10 and 17) 55 2.1.6.1 4-Hydroxybenzoic acid (4) 55 2.1.6.2 Vanillic acid (5) 56 2.1.6.3 Syringic acid (6) 57 2.1.6.4 Sucrose (7) 58 2.1.6.5 Salicylic acid (10) 60 2.1.6.6 (E)-p-Coumaric acid (17) 61 參、實驗部分 62 3.1儀器與材料 62 3.1.1理化性質測定儀器 62 3.1.2成分分離之儀器及材料 62 3.1.3試劑與溶媒 64 3.2植物來源 65 3.3狹葉赤車使者地上部成分萃取與純化 65 3.3.1 乙酸乙酯可溶部分之分離 66 3.3.1.1 化合物1和2之分離 67 3.3.1.2 化合物3之分離 67 3.3.1.3 化合物4~6和10之分離 67 3.3.2 正丁醇可溶部分之分離 68 3.3.2.1 化合物7~9之分離 69 3.3.2.2 化合物10~12之分離 69 3.3.2.3 化合物13~16之分離 70 3.4大冷水麻地上部成分萃取與純化 72 3.4.1 乙酸乙酯可溶部分之分離 73 3.4.1.1 化合物17、18之分離 73 3.4.2 甲醇沖提物之分離 73 3.4.2.1 化合物12、19、20之分離 73 3.4.3 2%乙酸不可溶部分之分離 74 3.4.3.1 化合物21之分離 74 3.5 化合物之物理數據 75 附圖 84 | |
dc.language.iso | zh-TW | |
dc.title | 狹葉赤車使者及大冷水麻地上部化學成分之研究 | zh_TW |
dc.title | Chemical constituents from the aerial parts of Elatostema lineolatum and Pilea melastomoides | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 陳益昇(Ih-Sheng Chen),林雲蓮(Yun-Lian Lin),張嘉銓(Chia-Chuan Chang) | |
dc.subject.keyword | 狹葉赤車使者,大冷水麻,化學成分, | zh_TW |
dc.subject.keyword | Elatostema lineolatum Wight var. majus Wedd.,Pilea melastomoides (Poir.) Wedd.,chemical constituent, | en |
dc.relation.page | 148 | |
dc.identifier.doi | 10.6342/NTU201902101 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2019-07-30 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 藥學研究所 | zh_TW |
顯示於系所單位: | 藥學系 |
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