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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 胡孟君(Meng-Chun Hu) | |
| dc.contributor.author | Yi-Jing Chen | en |
| dc.contributor.author | 陳怡靜 | zh_TW |
| dc.date.accessioned | 2021-05-19T17:56:37Z | - |
| dc.date.available | 2026-12-31 | |
| dc.date.available | 2021-05-19T17:56:37Z | - |
| dc.date.copyright | 2016-08-26 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-08-18 | |
| dc.identifier.citation | Atanasov AG, Leiser D, Roesselet C, Noti M, Corazza N, Schoonjans K, Brunner T. 2008. Cell cycle-dependent regulation of extra-adrenal glucocorticoid synthesis in murine intestinal epithelial cells. FASEB J 22: 4117-4125.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/7868 | - |
| dc.description.abstract | Cyp11a1基因在類固醇荷爾蒙生成過程中扮演著關鍵角色，在性腺控制性荷爾蒙生成，調控生殖作用。本論文利用Cyp11a1基因剔除小鼠（Cyp11a1-/-）探討類固醇對小鼠睪丸發育的影響。我們發現相較於野生型小鼠，8週大Cyp11a1-/-的睪丸重量小且無法正常沉降；此外，左側睪丸位置較右側高，重量也較小。Cyp11a1-/-睪丸細精管管徑小且觀察不到成熟精子，Leydig cell數量增加有肥大現象。免疫螢光染色分析結果顯示，Cyp11a1-/-細精管中沒有長型精細胞（elongated spermatid），僅在極少數細精管觀察到少量的圓形精細胞（round spermatid），另有大量生殖母細胞（spermatocyte）累積的現象，主要停留在粗絲期（pachytene）階段，少數則停在偶線（zygotene）階段。似乎有 較多未分化的精原幹細胞（undifferentiated spermatogonia stem cell）數量在Cyp11a1-/-細精管中，出生3天的Cyp11a1-/-生殖母細胞（gonocyte）的數量也多於Cyp11a1+/+。TUNEL實驗顯示，8週Cyp11a1-/-生殖細胞的凋亡明顯地增加許多。這些結果說明，Cyp11a1基因對精子生成過程的減數分裂時期是很重要的。
有些週邊組織亦有自行生成類固醇荷爾蒙的能力，已知Cyp11a1基因會表現在小腸，因此我們也觀察Cyp11a1-/-剔除對小腸的影響。在8週Cyp11a1-/-小腸絨毛，我們看到一些異常現象，包括，腸上皮細胞增厚為多層，上皮細胞與lamina propria脫離，微血管充血以及疑似免疫細胞浸潤。經BrdU注射在3天和8週小鼠小腸觀察到Cyp11a1-/-crypt細胞增生明顯少於Cyp11a1+/+。這些結果說明Cyp11a1基因在小鼠小腸發育可能扮演著重要的調控角色。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-05-19T17:56:37Z (GMT). No. of bitstreams: 1 ntu-105-R03441001-1.pdf: 4124749 bytes, checksum: 1bdcae0d13a6a49b0359aedaddafa72d (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 目錄 II
表次 IV 圖次 V 中文摘要 VI 英文摘要 VII 第一章 導論 1 一、 類固醇荷爾蒙之生成 1 二、 睪丸 1 1. 睪丸結構 1 2. 精子生成（spermatogenesis） 2 3. 雄性素（Androgen）對精子生成的重要性 3 三、 小腸 3 1. 醣皮質類固醇（glucocorticoid）對小腸的影響 4 四、 研究動機與目的 5 第二章 材料與方法 7 一、 基因轉殖小鼠 7 1. Cyp11a1+/-異合子 7 2. Cyp11a1-/- 基因剔除小鼠 7 3. Cyp11a1-/- 腎上腺移植 7 二、 小鼠基因型鑑定（Genotyping） 8 三、 BrdU（5-Bromo-2-deoxyuridine）處理 9 四、 灌流 9 五、 冷凍切片 10 1. 睪丸 10 2. 小腸組織 10 六、 石蠟組織切片（Paraffin Section） 10 七、 蘇木素-伊紅（hematoxylin-eosin，HE）染色 11 八、 Periodic acid-Schiff（PAS）染色 11 九、 免疫組織螢光染色 11 十、 BrdU螢光染色 13 十一、 細胞凋亡（apoptosis）分析 13 十二、 統計分析 13 第三章 結果 14 一、 Cyp11a1-/-小鼠產生 14 二、 Cyp11a1基因剔除對睪丸發育的影響 14 三、 Cyp11a1基因剔除對精子生成（spermatogenesis）的影響 15 1. 精細胞（spermatid） 15 2. 精母細胞（spermatocyte） 16 3. 未分化的精原幹細胞（Undifferentiated spermatogonia stem cell） 17 4. 生殖母細胞（Gonocyte） 17 5. 賽氏細胞（Sertoli cell） 17 四、 Cyp11a1基因剔除影響睪丸細胞增生與凋亡 18 五、 Cyp11a1基因剔除對小鼠小腸型態的影響 18 六、 Cyp11a1基因剔除對小腸細胞增生與凋亡的影響 19 第四章 討論 20 一、 Cyp11a1基因剔除小鼠睪丸左右兩側不正常沉降 20 二、 比較雄性Cyp11a1基因剔除與Androgen receptor剔除小鼠 21 三、 Cyp11a1基因剔除小鼠睪丸中Leydig cell型態肥大 22 四、 Cyp11a1在小腸中所扮演的角色 22 參考文獻 24 | |
| dc.language.iso | zh-TW | |
| dc.title | Cyp11a1基因剔除對小鼠睪丸與小腸發育之影響 | zh_TW |
| dc.title | Effects of Cyp11a1 knockout on the development of mouse testis and intestine | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 鍾邦柱(Bon-chu Chung),林盈宏(Ying-Hung Lin),李立仁(Li-Jen Lee) | |
| dc.subject.keyword | Cyp11a1,睪丸,生殖細胞,小腸, | zh_TW |
| dc.subject.keyword | Cyp11a1,testis,germ cells,intestine, | en |
| dc.relation.page | 55 | |
| dc.identifier.doi | 10.6342/NTU201603243 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2016-08-18 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 生理學研究所 | zh_TW |
| dc.date.embargo-lift | 2026-12-31 | - |
| 顯示於系所單位： | 生理學科所 | |
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