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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 吳益群 | zh_TW |
| dc.contributor.advisor | Yi-Chun Wu | en |
| dc.contributor.author | 謝宜岑 | zh_TW |
| dc.contributor.author | Yi-Cen Xie | en |
| dc.date.accessioned | 2021-07-11T15:01:48Z | - |
| dc.date.available | 2024-08-19 | - |
| dc.date.copyright | 2019-08-26 | - |
| dc.date.issued | 2019 | - |
| dc.date.submitted | 2002-01-01 | - |
| dc.identifier.citation | Arda, H.E., Taubert, S., MacNeil, L.T., Conine, C.C., Tsuda, B., Van Gilst, M., Sequerra, R., Doucette-Stamm, L., Yamamoto, K.R., and Walhout, A.J. (2010). Functional modularity of nuclear hormone receptors in a Caenorhabditis elegans metabolic gene regulatory network. Mol Syst Biol 6, 367.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78522 | - |
| dc.description.abstract | 飲食對於維持生物體脂肪恆定的影響甚鉅，因為食物不僅提供能量，其中所含的營養物質亦能調控脂肪之代謝過程。生物體分解脂肪能力下降造成了肥胖，進而引發糖尿病、心血管疾病及相關癌症的發生。雖然已知許多胞器如油滴、過氧化物酶體、溶酶體與粒線體能參與脂肪的降解，但是食物如何調控這些胞器去啟動分解脂肪的機制目前並不清楚。我們先前的實驗表明，將線蟲餵食在不同細菌食物下，線蟲體內脂肪含量並不相同。藉由光學顯微鏡觀察染色之胞器和具有特定胞器的螢光標記之線蟲，並輔以電子顯微鏡合併觀察，結果顯示攝取不同的細菌食物會對特定胞器之數量及型態產生影響。另外，我們亦發現部分酵素會在該特定胞器中影響與飲食相關的脂肪堆積。概括上述結果，本研究顯示飲食可主導特定胞器以調節宿主的脂肪代謝。 | zh_TW |
| dc.description.abstract | Diets have a great impact on lipid homeostasis of an organism. In addition to providing energy, nutrients of diets regulate lipid metabolism. Imbalance in lipid homeostasis has been associated with obesity, resulting in many health issues, including diabetes, heart diseases, and certain cancers. While several organelles such as lipid droplet, peroxisome, lysosome and mitochondria, participate in lipid breakdown, how diets modulate lipid degradation in relation to these organelles remains to be elucidated. We fed C. elegans different bacterial diets and found C. elegans had different levels of fat in the intestine. Using vital dyes and specific organelle markers in combination with light and electron microscopy, we showed that bacterial diets affect specific organelle numbers and morphology. In addition, we identified a few enzymes localized to the specific organelles in diet-dependent fat storage. Altogether, our results present a model that diets affect fat metabolism through specific enzymes in specific organelles. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-11T15:01:48Z (GMT). No. of bitstreams: 1 ntu-108-R06b43010-1.pdf: 4551066 bytes, checksum: e113c30a006434e9a9880e50d760b2a8 (MD5) Previous issue date: 2019 | en |
| dc.description.tableofcontents | 致謝 i
摘要 ii Abstract iii Introduction 1 Materials and Methods 11 Worm strains and husbandry 11 Bacteria strains 11 Worm synchronization 11 Oil Red O staining and quantification 12 Western blotting 13 Pulse-chase experiment 14 Fatty acid supplement 15 LysoTracker staining and quantification 15 Transmission electron microscopy 16 Vitamin B12 supplement on killed or lived bacteria 17 Results 18 DA1877 is dominant for reduced lipid content in the triacylglycerol form in C. elegans compared to OP50-fed ones 18 Fatty acids accumulate in lipid droplets (LDs) and lysosome-related organelles (LROs) to different extent in response to different diets 20 DA1877 diet-fed worms show more LROs in the intestine 21 Loss of LROs increases lipid content in DA1877-fed worms 22 Diets-mediated LRO density difference might be associated with autophagy activity 23 mdt-15 regulates LRO biogenesis in response to DA1877 25 Mitochondrial morphology is distinct under different bacterial diets while morphology is not correlated to lipid content 26 mmcm-1 is required for vitamin B12 induced lipid reduction 27 Discussion 30 Pulse-chase experiment of BODIPY –C12 30 MDT-15 functions as a regulator of lipid homeostasis 31 Organelle-organelle interaction in cells 32 The dietary effects of Vitamin B12 and other molecules on C. elegans 34 Mitochondrial dynamics and the activity 35 Figures 36 Fig.1 Pulse-chase assay of BODIPY-C12 present fatty acids appear in LDs and LROs 42 Fig.2 DA1877-fed worms had numerous LROs in the intestine 45 Fig.3 Loss of LRO causes lipid increasing under DA1877 feeding 48 Fig. 4 Endosomal trafficking is not altered in different diets fed worms 50 Fig. 5 Mutations in autophagy genes increased LRO density in OP50-fed animals 52 Fig. 6 DA1877 induced LRO biogenesis is regulated by mdt-15 53 Fig. 7 OP50 and DA1877 brought out distinct mitochondrial morphologies in the intestine of worms 55 Fig. 8 Nematodes reduced fat content in response to vitamin B12 through mmcm-1 dependent pathway 57 Fig. 9 The schematic diagram of fatty acid mobilization and its relation to organelles 58 Supplementary 59 Supplementary figure. 1 59 Supplementary figure. 2 60 Supplementary table. 1 61 Supplementary table. 2 62 Supplementary table. 3 62 Supplementary table. 4 63 Supplementary table. 5 64 Supplementary table. 6 65 Supplementary table. 7 66 Supplementary table. 8 67 Supplementary table. 9 68 Supplementary table. 10 69 Supplementary table. 11 70 Supplementary table. 12 71 References 72 | - |
| dc.language.iso | en | - |
| dc.title | 飲食調節線蟲脂肪之移動及其與相關胞器的關係 | zh_TW |
| dc.title | Lipid mobilization regulated by diets and its relation to organelles | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 107-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 王昭雯;陳昌熙;金翠庭 | zh_TW |
| dc.contributor.oralexamcommittee | Chao-Wen Wang;Chang-Shi Chen;Tsiu-Ting Ching | en |
| dc.subject.keyword | 秀麗隱桿線蟲,脂肪,肥胖,胞器,飲食, | zh_TW |
| dc.subject.keyword | C. elegans,lipid,fat,organelle,diets, | en |
| dc.relation.page | 75 | - |
| dc.identifier.doi | 10.6342/NTU201904032 | - |
| dc.rights.note | 未授權 | - |
| dc.date.accepted | 2019-08-19 | - |
| dc.contributor.author-college | 生命科學院 | - |
| dc.contributor.author-dept | 分子與細胞生物學研究所 | - |
| dc.date.embargo-lift | 2024-08-26 | - |
| 顯示於系所單位： | 分子與細胞生物學研究所 | |
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