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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78313完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張智芬(Zee-Fen Chang) | |
| dc.contributor.author | Shin-Mei Liu | en |
| dc.contributor.author | 劉炘莓 | zh_TW |
| dc.date.accessioned | 2021-07-11T14:50:43Z | - |
| dc.date.available | 2025-08-06 | |
| dc.date.copyright | 2020-09-07 | |
| dc.date.issued | 2020 | |
| dc.date.submitted | 2020-08-06 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78313 | - |
| dc.description.abstract | 核苷二磷酸激酶3(NME3) 是屬於苷二磷酸激酶 (NDPK) 家族的其中一員，已知 NME3存在粒線體外膜上，也是一個潛在的組氨酸激酶 (Histidinekinase)。從乳癌 患者臨床數據中發現核苷二磷酸激酶 3 的表現量與患者的預後呈負相關。我們發 現在高度轉移性乳癌細胞中，例如 MDA-MB-231 細胞，具有較低的 NME3 表現 量。在 MDA-MB-231 細胞中增加完整的 NME3 表現量，會抑制 MDA-MB-231 細 胞的移動、侵襲與轉移，然而增加 N 端去除的 NME3 表現量卻無效果。我們證明 了 NME3 會降低粒線體的短桿狀 (fragmentation)、絲狀偽足 (filopodia) 的生成 以及肌凝蛋白輕鏈 2 (MLCII) 的磷酸化。為了釐清 NME3 如何同時影響這些分子 事件，我們比較表現不同型的 NME3 在細胞中，包含野生型 (wild-type) NME3、 酵 素 活 性 抑 制 型 (catalytic-dead) NME3 oligomerization defective) NME3。在 MDA-MB-231 中，酵素活性抑制的 NME3 會 filopodia 的生成，但維持了 RhoA-ROCK 所調控的 MLCII 磷酸化。在 NME3 表現的細胞中，阻止粒線體融合 (fusion) 而干擾 NME3 調 控的粒線體延長可以恢復細胞的移動和侵襲能力，但在野生型 NME3 表現的細胞 中則無此現象。相反地， 組氨酸磷酸化 (histidine phosphorylation) 的 filopodia 的生 成和 MLCII 磷酸化皆下降，進而抑制細胞移動及侵襲的能力。根據這些結果，我 們推測 NME3 存在兩種不同的功能以抑制細胞移動及侵襲所需的 filopodia 的生 成，其一是藉由 NME3 的聚合促進粒線體的延長，另外則是藉由 NME3 的組氨酸 激酶功能抑制 RhoA-ROCK-MLC II 路徑。 | zh_TW |
| dc.description.abstract | NME3, termed as non-metastasis enzyme (NME), is a nucleoside diphosphate kinase (NDPK). Given the presence of high-energy-phosphate intermediate at the catalytic histidine residue, NME3 is also a potential histidine kinase located on mitochondrial outer membrane. Data mining from databases of clinical specimens of breast cancer patients demonstrated that the expression level of NME3 was correlated with poor prognosis. We found that highly metastatic breast cancer cell lines such as MDA-MB-231 cells express lower level of NME3. Increased expression of full-length of NME3 inhibited MDA-MB- 231 cells in migration, invasion and in vivo metastasis, while NME3 deleted of N- terminus region had no effect. We showed NME3-mediated reduction in mitochondrial fragmentation, filopodia formation, and myosin light chain II (MLCII) phosphorylation. Expression of wild-type, catalytic-dead, and oligomerization defective of NME3 all caused the loss of invasion capability in MDA-MB231. The expression of catalytic-dead mutant of NME3 led to mitochondrial elongation with decreased amount of filopodia while retaining RhoA-ROCK-mediated MLCII phosphorylation in MDA-MB-231 cells. Blocking mitochondrial fusion to interfere NME3-mediated mitochondrial elongation was able to restore migration and invasion in cells expressing catalytic-dead mutant but not wild-type. As for the cells expressing oligomerization mutant of NME3 defective in stimulation of mitochondrial elongation, the abilities in migration and invasion were still suppressed with reduction in filopodia formation and MLCII phosphorylation . According to these results, we proposed that NME3 exerts two different functions to suppress filopodia formation for migration and invasion, one by stimulating mitochondrial elongation via oligomerization and the other by suppressing RhoA-ROCK-MLCII pathway via the histidine kinase function. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-11T14:50:43Z (GMT). No. of bitstreams: 1 U0001-0508202017371200.pdf: 11372974 bytes, checksum: 65203ecdd923b4805024598edd0fac46 (MD5) Previous issue date: 2020 | en |
| dc.description.tableofcontents | 口試委員會審定書................................................................. i 謝誌.......................................................................... ii 論文摘要......................................................................iii Abstract .....................................................................iv Table of contents .............................................................1 Introduction ................................................................. 2 1.Nucleoside diphosphate kinase (NDPK, NME, Nm23) family ..................... 2 1.1 NME isoforms...............................................................2 1.2 NME1 and NME2 as metastasis suppressors....................................3 1.3 The function of NME3 in mitochondria.......................................4 1.4 The function of NME3 in cell motility .....................................5 Breast cancer ................................................................ 6 2.1 Breast cancer molecular subtype and treatments ............................6 2.2 Breast cancer metastasis...................................................8 2.3 Mitochondria dynamics regulate cancer cell metastasis .....................9 Materials and Methods.........................................................12 Result .......................................................................22 Increase expression of NME3 suppresses invasion process in MDA-MB-231 ....... 22 NME3 promotes mitochondrial elongation which is independent of catalytic function to suppress filopodia formation and invasion process in MDA-MB-231 ..24 Two separate routes in migration suppression by NME3..........................26 Enzymatic function of NME3 suppresses Myosin light chain phosphorylation .... 27 Total RhoA activity is not affected by NME3...................................28 NME3 locates on mitochondria and plasmamembrane via its N-terminal sequence ..29 Flag-NME3 interacts with NME1 ............................................... 30 Discussion....................................................................31 Figures ......................................................................36 Reference ....................................................................52 Appendix .................................................................... 61 | |
| dc.language.iso | en | |
| dc.subject | 粒線體 | zh_TW |
| dc.subject | 核苷二磷酸激酶3 | zh_TW |
| dc.subject | 乳癌 | zh_TW |
| dc.subject | 轉移 | zh_TW |
| dc.subject | ROCK | en |
| dc.subject | NME3 | en |
| dc.subject | Breast Cancer | en |
| dc.subject | Metastasis | en |
| dc.subject | mitochondria | en |
| dc.subject | RhoA | en |
| dc.title | 核苷二磷酸激酶 3 藉由雙重功能抑制乳癌細胞的轉移 | zh_TW |
| dc.title | The Dual Function of NME3 Suppresses Breast Cancer Metastasis | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 108-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 周祖述(Tzuu-Shuh Jou),蔡丰喬(Feng-Chiao Tsai),劉雅雯(Ya-Wen Liu),李曉暉(Hsiao-Hui Lee) | |
| dc.subject.keyword | 核苷二磷酸激酶3,乳癌,轉移,粒線體, | zh_TW |
| dc.subject.keyword | NME3,Breast Cancer,Metastasis,mitochondria,RhoA,ROCK, | en |
| dc.relation.page | 64 | |
| dc.identifier.doi | 10.6342/NTU202002489 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2020-08-07 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 分子醫學研究所 | zh_TW |
| dc.date.embargo-lift | 2025-08-06 | - |
| 顯示於系所單位： | 分子醫學研究所 | |
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