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Chemical Modification of Chitosan with Furan and Imidazole Functional Groups for the Application of Photo-Curable Adhesive Agents
Chitosan,Methylene blue,Photo-crosslink,Imidazole,Furan,658nm Red Laser,Adhesion,
|Publication Year :||2016|
Current technologies for reconnecting and sealing tissues after surgical procedures are most mechanical type fixed, such as sutures, wires, and staples. Those methods have several disadvantages. First, they are invasive procedures, create secondary damage surrounding tissue of wound, and let patients suffer more pain. More complicated processes make the use of those ways for wound closure is time consuming. It cannot seal wound absolutely, having the problem of infection, and do not stop body fluid and air leakages. Consequently, the novel tissue adhesion glue, surgical adhesive biomaterials, instead sutures, wires, and staples.
We hope to make tissue adhesive agent that can control curing time, select chitosan as basic substrate, and utilize EDC/NHS reaction to introduce furan/imidazole functional groups, which are sensitive to radiation energy. Choose Methylene blue as photosensitizer, and use red laser diode (658nm, 100mW). The modified-chitosan is radiated by red laser and initiate photo-curable reaction.
The modified-chitosan was analyzed by Nuclear Magnetic Resonance Spectroscopy (NMR), Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), and UV-vis absorbance Spectrum to confirm chemical structure, found its physical strength and stability by tension test and repeatable swelling test. Finally, We examined cytotoxicity of the modified-chitosan by MTT assay.
The result showed that introducing furan/imidazole functional groups into chitosan actually made chitosan become photo-crosslinkable. We can exploit photo radiation to control the initial time of crosslink and acquire a stable linkage. Through the MTT assay, the result proved that the modified-chitosan is bio-acceptable. While, the tension test showed it has weak strength, which was not suitable for tissue adherence, it may be improved by using chitosan with lower molecular weights or higher degree of deacetylation ratio in the future or we can apply this material into different field, such as wound healing, tissue engineering. In conclusion, this photo-curable chitosan still has its potential for future development.
|Appears in Collections:||高分子科學與工程學研究所|
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