利用雙光子螢光顯微鏡之影像分析肺腺癌細胞核質比

Abstract

肺腺癌屬於非小細胞癌，主要的來源是肺部外層的表皮細胞變異，因為早期癌變的狀態並非塊狀或腫瘤，亦無症狀，x-ray檢測發現的時機通常已是癌末。將癌末細胞從人體取出體外複製培養成具有穩定繼代特性細胞株(cell line)，再依據其轉移能力與惡化程度分成CL1-0與CL1-5。根據病理學，癌細胞的細胞核大小與細胞成熟度有關聯，在本研究中，我們利用螢光染劑的特性與雙光子螢光顯微鏡拍攝平面與不同深度的影像。藉由ImageJ分析這些影像資料，圈選細胞核與細胞輪廓得到面積的比例關係。發現CL1-5的細胞核面積較CL1-0大，NC比例則是CL1-0的大於CL1-5。

Adenocarcinoma cancer, a type of non-small cell lung cancer, is caused by the variation of the epithelial cells in the outer layer of lung. Because in the early state, the adenocarcinoma cancer does not form blocks nor cancerous tumor, may also be asymptomatic, the x-ray detectable cancer is usually in terminal state. The cancer cells derived from patients were replicated in vitro and cultured into cell lines. According to their invasive ability and degree of deterioration, the cell lines were divided into CL1-0 and CL1-5. Based on pathology, the cell nuclei size is associated with the maturity of the cancer cells. In this study, we used fluorescent dye properties and two-photon fluorescence microscopy to image these two types of cells at different depths. By using ImageJ, we analyzed the image data, circling the nucleus and cell outline to obtain the proportion of nucleus to cytoplasm. In my research the nuclei area of CL1-5 is bigger than CL1-0; the nuclear-to-cytoplasmic ratio (NC ratio) of CL1-0 is greater than CL1-5.